The present study provides a comparative understanding of florfenicol deposition kinetics following oral administration at a single dose of 30 mg/kg body weight to Japanese eel （ Anguilla japonica） at the temperatur...The present study provides a comparative understanding of florfenicol deposition kinetics following oral administration at a single dose of 30 mg/kg body weight to Japanese eel （ Anguilla japonica） at the temperature of 20℃, 24℃ and 28℃, respectively. Approximate 0.3 mL of blood sample from each eel in these three groups was collected in a row at the time 0.5, 1, 2, 4, 8, 12, 24 and 36 h after medicated-feed gavage. The concentrations of florfenicol in eel plasma were detected by reversed-phase high performance liquid chromatography （ RP - HPLC ）. The plasma concentration-time data of each eel were analyzed by non-compartmental methods based on statistical moment theory using DAS software. Pharmacokinetics parameters of different groups were tested by one-way analysis of variance using SPSS software. It was found that most of parameters were different significantly among groups （ P 〈 0.05 ）. In the three groups at 20℃, 24℃ and 28℃, pharmacokinetics parameters of peak plasma concentration （ Cmax ） were （7. 839 ± 1. 125）, （ 13. 010 ± 2. 334） and （ 18. 267 ± 3. 717 ） μg/mL, and the time to reach the Cmax（Tmax） were （6.500± 2.070）, （4.500 ± 1.414） and （3.429 ± 0.926） h, respectively. These suggested that eels at higher temperature absorbed more drug and more quickly. The volumes of distribution （Vz/F） were （3. 964 ± 0. 594）, （2. 466 ± 0. 672） and （1. 841 ± 0.485） IZkg, respectively. The difference deduced that more florfenicol was bound to tissue in eels at lower temperature. The mean residence time （ MRT0-∞ ） and the half-life of drug （ t1/2, ） in the three respective groups were （31.503 ±7.117）, （22.881 ± 4.940） and （22.134 ± 6.204） h, and （21.243 ±5.166）, （14.994±4.293） and （14. 656± 5. 061 ） h. These parameters showed that the elimination rate of florfenicol in eels at 24℃ or 28℃ was more quickly than that at 20℃. The areas under the concentration -time curve （AUC0-∞ ） were （235.580 ±62.013）, （271.983±75.023） and （353. 192 ± 92.491） μg h/mL, respectively. It indicated that the relative bioavailability of florfenicol was higher significantly in eels at higher temperature.展开更多
基金Fund on Special Fund for Agro-scientific Research in the Public Interest(201203085)Natural Science Foundation Project of Fujian(2008J0069)
文摘The present study provides a comparative understanding of florfenicol deposition kinetics following oral administration at a single dose of 30 mg/kg body weight to Japanese eel （ Anguilla japonica） at the temperature of 20℃, 24℃ and 28℃, respectively. Approximate 0.3 mL of blood sample from each eel in these three groups was collected in a row at the time 0.5, 1, 2, 4, 8, 12, 24 and 36 h after medicated-feed gavage. The concentrations of florfenicol in eel plasma were detected by reversed-phase high performance liquid chromatography （ RP - HPLC ）. The plasma concentration-time data of each eel were analyzed by non-compartmental methods based on statistical moment theory using DAS software. Pharmacokinetics parameters of different groups were tested by one-way analysis of variance using SPSS software. It was found that most of parameters were different significantly among groups （ P 〈 0.05 ）. In the three groups at 20℃, 24℃ and 28℃, pharmacokinetics parameters of peak plasma concentration （ Cmax ） were （7. 839 ± 1. 125）, （ 13. 010 ± 2. 334） and （ 18. 267 ± 3. 717 ） μg/mL, and the time to reach the Cmax（Tmax） were （6.500± 2.070）, （4.500 ± 1.414） and （3.429 ± 0.926） h, respectively. These suggested that eels at higher temperature absorbed more drug and more quickly. The volumes of distribution （Vz/F） were （3. 964 ± 0. 594）, （2. 466 ± 0. 672） and （1. 841 ± 0.485） IZkg, respectively. The difference deduced that more florfenicol was bound to tissue in eels at lower temperature. The mean residence time （ MRT0-∞ ） and the half-life of drug （ t1/2, ） in the three respective groups were （31.503 ±7.117）, （22.881 ± 4.940） and （22.134 ± 6.204） h, and （21.243 ±5.166）, （14.994±4.293） and （14. 656± 5. 061 ） h. These parameters showed that the elimination rate of florfenicol in eels at 24℃ or 28℃ was more quickly than that at 20℃. The areas under the concentration -time curve （AUC0-∞ ） were （235.580 ±62.013）, （271.983±75.023） and （353. 192 ± 92.491） μg h/mL, respectively. It indicated that the relative bioavailability of florfenicol was higher significantly in eels at higher temperature.
