AIM To study the reversing effect of Chinese drug tanshinone on malignant phenotype of cancer cells.METHODS Human hepatocarcinoma cell line (SMMC-7721) was treated in vitro with 0.5mg/L tanshinone for 4 days, and vari...AIM To study the reversing effect of Chinese drug tanshinone on malignant phenotype of cancer cells.METHODS Human hepatocarcinoma cell line (SMMC-7721) was treated in vitro with 0.5mg/L tanshinone for 4 days, and variation in cell differentiation was detected.RESULTS The morphology of cancer cells was tended toward well differentiation and cell growth was markedly inhibited. BrdU uptake assay and immunohistochemical stain of PCNA showed that the BrdU labeling rate and PCNA positive rate were lower than the controls, but no difference was found statistically as compared with all transretinoic acid. Flow cytometric assay demonstrated that S phase cells decreased and G0/G1 phase cells increased. Expression of c-myc oncogene protein decreased but the c-fos oncogene protein markedly increased.CONCLUSION Tanshinone could reverse the inducing differentiation in human hepatocarcinoma cells (SMMC-7721). It may become a new prospective inducer of cell differentiation to treat cancers.展开更多
目的探讨雌激素在胃癌发生、发展中的作用及临床意义.方法应用流式细胞术检测31例胃癌组织、10例正常胃组织雌激素受体(estrogen receptor,ER)和细胞周期蛋白 D_1(cyclinD_1)表达量;应用免疫组织化学法检测31例胃肠癌组织、10例正常胃...目的探讨雌激素在胃癌发生、发展中的作用及临床意义.方法应用流式细胞术检测31例胃癌组织、10例正常胃组织雌激素受体(estrogen receptor,ER)和细胞周期蛋白 D_1(cyclinD_1)表达量;应用免疫组织化学法检测31例胃肠癌组织、10例正常胃肠组织中 ER 和增殖细胞核抗原(proliferatingcell nuclear,PCNA)蛋白表达.结果流式细胞术定量检测结果表明,胃癌组织 ER 表达量(FI,1.19±0.23)明显高于正常胃组织(FI,1.00±0.04,P<0.01);胃癌组织 cyclinD_1表达量(FI,1.33±0.17)明显高于正常胃组织(FI,1.00±0.09,P<0.01).胃癌组织 ER 表达量与 cyclinD_1表达量存在正相关关系(r_s=0.40,P<0.05).免疫组织化学染色结果表明,胃癌组织 ER 表达阳性率为45%(14/31),明显高于正常胃组织0%(0/10,P<0.01);胃癌组织 PCNA 蛋白表达阳性率为97%(30/31),明显高于正常胃组织0%(0/10,P<0.01),且胃癌组织 ER 表达等级与 PCNA 蛋白表达等级间存在正相关关系(r_s=0.44,P<0.05).结论雌激素在胃癌发生、发展中有促进细胞增殖的作用,部分胃癌很有可能是雌激素依赖性肿瘤.展开更多
目的了解供肝复流后细胞增殖周期的变化,明确药物预处理和缺血预处理对其的影响.方法建立大鼠三袖套法原位肝移植模型,观察移植肝细胞复流前后不同时间增殖细胞核抗原的表达情况;对供肝分别作药物预处理和缺血预处理,比较正常肝、预处...目的了解供肝复流后细胞增殖周期的变化,明确药物预处理和缺血预处理对其的影响.方法建立大鼠三袖套法原位肝移植模型,观察移植肝细胞复流前后不同时间增殖细胞核抗原的表达情况;对供肝分别作药物预处理和缺血预处理,比较正常肝、预处理供肝和未预处理供肝复流2 h 后的增殖细胞核抗原、肝酶谱及肝细胞凋亡状况.结果移植肝细胞复流2 h 后增殖细胞核抗原表达显著增高,12 h 后恢复正常水平;正常肝、药物预处理供肝、缺血预处理供肝、未预处理供肝复流2 h 后的增殖细胞核抗原表达和肝酶谱依次升高,PCNA(%):2.1±0.1,4.3±0.2,7.0±0.4,9.4±0.4,AST(nKat·L^(-1)):3.5±1.6,28.1±11.9,53.8±12.3,75.2±24.1,ALT(nKat·L^(-1)):2.8±2.7,46.1±17.6,61.7±22.9,90.5±30.1,LDH(nKat·L^(-1)):49.6±7.6,61.4±15.3,95.4±23.2,148.3±30.1.结论供肝复流后肝细胞 G_1期缩短至2 h 以内,整个细胞周期时间缩短至20 h 左右.正常肝、药物预处理供肝、缺血预处理供肝、未预处理供肝肝损害程度依次加重,整个细胞周期时间依次缩短.展开更多
基金卫生部科研项目,Grant of China Medical Board of New York,INC。
文摘AIM To study the reversing effect of Chinese drug tanshinone on malignant phenotype of cancer cells.METHODS Human hepatocarcinoma cell line (SMMC-7721) was treated in vitro with 0.5mg/L tanshinone for 4 days, and variation in cell differentiation was detected.RESULTS The morphology of cancer cells was tended toward well differentiation and cell growth was markedly inhibited. BrdU uptake assay and immunohistochemical stain of PCNA showed that the BrdU labeling rate and PCNA positive rate were lower than the controls, but no difference was found statistically as compared with all transretinoic acid. Flow cytometric assay demonstrated that S phase cells decreased and G0/G1 phase cells increased. Expression of c-myc oncogene protein decreased but the c-fos oncogene protein markedly increased.CONCLUSION Tanshinone could reverse the inducing differentiation in human hepatocarcinoma cells (SMMC-7721). It may become a new prospective inducer of cell differentiation to treat cancers.
文摘目的探讨雌激素在胃癌发生、发展中的作用及临床意义.方法应用流式细胞术检测31例胃癌组织、10例正常胃组织雌激素受体(estrogen receptor,ER)和细胞周期蛋白 D_1(cyclinD_1)表达量;应用免疫组织化学法检测31例胃肠癌组织、10例正常胃肠组织中 ER 和增殖细胞核抗原(proliferatingcell nuclear,PCNA)蛋白表达.结果流式细胞术定量检测结果表明,胃癌组织 ER 表达量(FI,1.19±0.23)明显高于正常胃组织(FI,1.00±0.04,P<0.01);胃癌组织 cyclinD_1表达量(FI,1.33±0.17)明显高于正常胃组织(FI,1.00±0.09,P<0.01).胃癌组织 ER 表达量与 cyclinD_1表达量存在正相关关系(r_s=0.40,P<0.05).免疫组织化学染色结果表明,胃癌组织 ER 表达阳性率为45%(14/31),明显高于正常胃组织0%(0/10,P<0.01);胃癌组织 PCNA 蛋白表达阳性率为97%(30/31),明显高于正常胃组织0%(0/10,P<0.01),且胃癌组织 ER 表达等级与 PCNA 蛋白表达等级间存在正相关关系(r_s=0.44,P<0.05).结论雌激素在胃癌发生、发展中有促进细胞增殖的作用,部分胃癌很有可能是雌激素依赖性肿瘤.
文摘目的了解供肝复流后细胞增殖周期的变化,明确药物预处理和缺血预处理对其的影响.方法建立大鼠三袖套法原位肝移植模型,观察移植肝细胞复流前后不同时间增殖细胞核抗原的表达情况;对供肝分别作药物预处理和缺血预处理,比较正常肝、预处理供肝和未预处理供肝复流2 h 后的增殖细胞核抗原、肝酶谱及肝细胞凋亡状况.结果移植肝细胞复流2 h 后增殖细胞核抗原表达显著增高,12 h 后恢复正常水平;正常肝、药物预处理供肝、缺血预处理供肝、未预处理供肝复流2 h 后的增殖细胞核抗原表达和肝酶谱依次升高,PCNA(%):2.1±0.1,4.3±0.2,7.0±0.4,9.4±0.4,AST(nKat·L^(-1)):3.5±1.6,28.1±11.9,53.8±12.3,75.2±24.1,ALT(nKat·L^(-1)):2.8±2.7,46.1±17.6,61.7±22.9,90.5±30.1,LDH(nKat·L^(-1)):49.6±7.6,61.4±15.3,95.4±23.2,148.3±30.1.结论供肝复流后肝细胞 G_1期缩短至2 h 以内,整个细胞周期时间缩短至20 h 左右.正常肝、药物预处理供肝、缺血预处理供肝、未预处理供肝肝损害程度依次加重,整个细胞周期时间依次缩短.
