类风湿性关节炎患者T细胞亚群免疫荧光流式细胞分析

目的：探讨Ｔ细胞亚群与类风湿性关节炎（Ｒｈｅｕｍａｔｏｉｄａｒｔｈｒｉｔｉｓ，ＲＡ）的关系及类风湿性关节炎的发生发展变化与细胞免疫功能的关系。方法：采用免疫荧光染色法和流式细胞仪检测５６例ＲＡ患者及４５例正常人外周血ＣＤ４＋、ＣＤ８＋细胞数。结果：正常人血浆ＣＤ４＋细胞数（４３．５２±２．１６）％，ＣＤ８＋细胞数（２２．３８±１．９８）％，ＲＡ患者ＣＤ４＋（４５．１８±３．２７）％，ＣＤ８＋（１５．０５±３．１５）％。ＣＤ４＋／ＣＤ８＋比值较正常人高（Ｐ＜０．０５），且ＲＡ患者中活动期与非活动期相比亦有显著性差异（Ｐ＜０．０５）。结论：本研究提示细胞免疫状态参与了ＲＡ的发病过程；进一步了解其作用机理，对ＲＡ的防治具有重要意义。

应用流式细胞仪检测大肠癌患者外周血淋巴细胞IL一2受体的表达

应用流式细胞仪检测２２例大肠癌患者外周血淋巴细胞ＩＬ一２受体（ＩＬ一２Ｒ）表达。结果表明：大肠癌患者ＩＬ一２Ｒ表达能力明显低下，低下程度与肿瘤病期早晚有关，而与肿瘤部位无关。早期大肠癌（ＤｕｋｅｓＡ，Ｂ期）ＩＬ一２Ｒ表达基本正常，而中晚期大肠癌（ＤｕｋｅｓＣ，Ｄ期）ＩＬ一２Ｒ表达显著低下。ＩＬ一２Ｒ表达低下可能会削弱肿瘤宿主对ＩＬ一２治疗的反应。

Neural cell adhesion molecule-180 expression as a prognostic criterion in colorectal carcinoma:Feasible or not?

AIM： To evaluate the frequency of neural cell adhesion molecule （NCAM）-180 expression in fresh tumor tissue samples and to discuss the prognostic value of NCAM-180 in routine clinical practice.METHODS： Twenty-six patients （16 men, 10 women） with colorectal cancer were included in the study. Fresh tumor tissue samples and macroscopically healthy proximal margins of each specimen were subjected to flow-cytometric analysis for NCAM-180 expression.RESULTS： Flow-cytometric analysis determined NCAM-180 expression in whole tissue samples of macroscopically healthy colorectal tissues. However, NCAM-180 expression was positive in only one case （3.84%） with well-differentiated Stage Ⅱ disease who experienced no active disease at 30 months follow-up. CONCLUSION： As a consequence of the limited number of cases in our series, it might not be possible to make a generalisation, nevertheless the routine use of NCAM-180 expression as a prognostic marker for colorectal carcinoma seems to be unfeasible and not cost-effective in clinical practice due to its very low incidence.

Hot Spring Hydro Therapy Regulates Peripheral Leukocyte Together with Emotional Hormone and Receptor Positive Lymphocytes According to Each Constitution/Condition

Two primary defense systems develop in vertebrates, innate and adaptive. Despite those defense systems, the overwhelming problems of possessing this dual system, the innate or adoptive do not seem to guard or even prevent the development of one internal threat to survival, moreover sometimes direct to autoimmunity accompanying hypersensitivity. Further, every individual exposes to the risk of immunodeficiency in daily life with both internal and external stimuli. In this report, we tried to report the suitable menu for regulating the immune system modulate and discuss how we can compare and select each menu by evidence-based manner more than VAS. In a series of investigation, we have assessed the various health promoting menus such as hot spring hydrotherapy, acupuncture & moxibustion, light walking, independent to the western medicine. In this report, we try to summarize the regulatory effect of hot spring hydrotherapy as our representative menu under the influence of hypothalamus system. Their effects of elements were evaluated by the total number of peripheral leukocyte, its subsets, granulocyte and lymphocyte ratio, emotional hormones, receptor positive lymphoid cells. The regulative effects were confirmed also by other menu than hydrotherapy, acupuncture, walking etc. However, just by the simple comparison between the group before and after the hydrotherapy, we cannot exhibit the real regulatory effect by each menu. So we tried to show the effect by trying to indicate the constitution/condition dependent manner. Because the effect was not uniformly to each individual but it was dependent on each condition/constitution before the start of the menu. This is the one of the main proposes of this report for summaries that it is important to regulate the number and levels of each factor as ideal value. In other words, the regulation of each factor affected under the hypothalamus system is regulated with each constitution, under the influence of daily circumstances. The mode of regulation was the same in each menu, indicating that the higher leveler was down regulated and lower leveler was up regulated. Moreover, each vector of change was reversely correlated to the value of the day before. The other purpose of this study was designed to establish the regulatory effect of this hydrotherapy being not limited in the number of leukocyte in peripheral but in the emotional hormones. They were adrenaline and dopamine but not found another hormone for healthy individuals. Hot spring hydrotherapy for a short duration was expected to influence the immune system combining with hormone levels in the blood quantitatively. These hormones were evidenced by adrenalin and dopamine as well as adrenergic receptor positive lymphocytes. The final subject was to confirm and compare the effect of hot spring hydrotherapy and other menu. The reproducible effects were expressed by the linear slant and could compare each other with the value of slope. We tried to discuss the advantage and demerit, how we can compare each medicine with the peripheral leukocyte in number and function.

Anopheles gambiae strain(Ag55)cultured cells originated from Anopheles coluzzii and are phagocytic with hemocyte-like gene expression

Anopheles gambiae and Anopheles coluzzii are closely related species that are predominant vectors of malaria in Africa.Recently,A.gambiae form M was renamed A.coluzzii and we now conclude on the basis of a diagnostic PCR-restriction fragment length polymorphism assay that Ag55 cells were derived from A.coluzzii.We established an Ag55 cell transcriptome,and KEGG pathway analysis showed that Ag55 cells are enriched in phagosome pathway transcripts.The Ag55 transcriptome has an abundance of specific transcripts characteristic of mosquito hemocytes.Functional E.coli bioparticle uptake experiments visualized by fluorescence microscopy and confocal microscopy and quantified by flow cytometry establish the phagocytic competence of Ag55 cells.Results from this investigation of Ag55 cell properties will guide researchers in the use and engineering of the Ag55 cell line to better enable investigations of Plasmodium,other microbes,and insecticidal toxins.