Plankton size structure is crucial for understanding marine ecosystem dynamics and the associated biogeochemical processes.A fixation step by acid Lugol’s solution has been commonly employed to preserve plankton samp...Plankton size structure is crucial for understanding marine ecosystem dynamics and the associated biogeochemical processes.A fixation step by acid Lugol’s solution has been commonly employed to preserve plankton samples in the field.However,the acid Lugol’s solution can bias the estimation of size structure and the preserved plankton size structure can vary with time.Here,we explore the impact of sample storage time on the size-structure of the plankton community preserved by Lugol’s solution.Two short-term experiments and one long-term experiment were conducted to explore the change of plankton community size structure with the storage time:covering from a week to a month,and to nearly seven months based on particle-size data obtained by continuous Flow Cytometer and Microscope(FlowCAM)measurements.We found a linear change of plankton size with the storage time in short-term periods(less than 3 months)with a decrease of the slope but an increase of the intercept for the normalized biomass size spectrum(NBS S).However,there were opposite trends for NBSS with increasing slope but decreasing intercept after3 months.The potential causes of the distinct patterns of the NBSS parameters are addressed in terms of the interplay between particle aggregation and fragmentation.We found large changes in plankton biovolume and abundance among different size classes,which may indicate a distinct effect of acid Lugol’s solution on various plankton size classes.The mechanism driving temporal change in the size-structure of the Lugolfixed plankton community was further discussed in terms of particle aggregation and fragmentation.Finally,we emphasize that the effect of storage time should be taken into account when interpreting or comparing data of plankton community acquired from samples with various storage durations.展开更多
采用流式细胞摄像系统(Flow Cytometer And Microscope,FlowCAM)技术,构建了福建南部海域的赤潮生物图谱数据库,并利用该数据库分别用VisualSpread软件自动识别法和描述子分析算法(MATLAB)来鉴定样品,对两种方法的识别精确度进行了比较...采用流式细胞摄像系统(Flow Cytometer And Microscope,FlowCAM)技术,构建了福建南部海域的赤潮生物图谱数据库,并利用该数据库分别用VisualSpread软件自动识别法和描述子分析算法(MATLAB)来鉴定样品,对两种方法的识别精确度进行了比较。VisualSpreadsheet专业软件分析能力达到98.2%的平均精度,自主开发的特征描述子分类器达到94.1%的平均精度,拓展了实验设备的应用范围。展开更多
基金Supported by the Guangdong Province Special Support Plan for Leading Talents(No.2019TX05H216)the Key Special Project for Introduced Talents Team of Southern Marine Science and Engineering Guangdong Laboratory(Guangzhou)(No.GML2019ZD0305)+1 种基金the National Natural Science Foundation of China(No.41906132)the Science and Technology Program of Guangzhou(No.202102021229)。
文摘Plankton size structure is crucial for understanding marine ecosystem dynamics and the associated biogeochemical processes.A fixation step by acid Lugol’s solution has been commonly employed to preserve plankton samples in the field.However,the acid Lugol’s solution can bias the estimation of size structure and the preserved plankton size structure can vary with time.Here,we explore the impact of sample storage time on the size-structure of the plankton community preserved by Lugol’s solution.Two short-term experiments and one long-term experiment were conducted to explore the change of plankton community size structure with the storage time:covering from a week to a month,and to nearly seven months based on particle-size data obtained by continuous Flow Cytometer and Microscope(FlowCAM)measurements.We found a linear change of plankton size with the storage time in short-term periods(less than 3 months)with a decrease of the slope but an increase of the intercept for the normalized biomass size spectrum(NBS S).However,there were opposite trends for NBSS with increasing slope but decreasing intercept after3 months.The potential causes of the distinct patterns of the NBSS parameters are addressed in terms of the interplay between particle aggregation and fragmentation.We found large changes in plankton biovolume and abundance among different size classes,which may indicate a distinct effect of acid Lugol’s solution on various plankton size classes.The mechanism driving temporal change in the size-structure of the Lugolfixed plankton community was further discussed in terms of particle aggregation and fragmentation.Finally,we emphasize that the effect of storage time should be taken into account when interpreting or comparing data of plankton community acquired from samples with various storage durations.
文摘采用流式细胞摄像系统(Flow Cytometer And Microscope,FlowCAM)技术,构建了福建南部海域的赤潮生物图谱数据库,并利用该数据库分别用VisualSpread软件自动识别法和描述子分析算法(MATLAB)来鉴定样品,对两种方法的识别精确度进行了比较。VisualSpreadsheet专业软件分析能力达到98.2%的平均精度,自主开发的特征描述子分类器达到94.1%的平均精度,拓展了实验设备的应用范围。
