Eucommia ulmoides male flowers are rich in secondary metabolite,which have anti-tumor,sedative hypnotic,hypotensive,hypolipidemic,anti-fatigue,bacteriostatic,antioxidant and anti-aging effects.The conventional process...Eucommia ulmoides male flowers are rich in secondary metabolite,which have anti-tumor,sedative hypnotic,hypotensive,hypolipidemic,anti-fatigue,bacteriostatic,antioxidant and anti-aging effects.The conventional processing of E.ulmoides male flowers leads to the loss of nutrients and active ingredients.In recent years,cell wall breaking technology has been developed and utilized in various fields such as traditional Chinese medicine,good,chemical industry and biology.In order to promote the development of the E.ulmoides industry,the cell wall breaking technology and its characteristics are reviewed,and the application advantages of the cell wall breaking technology in the male flowers of E.ulmoides are discussed,and the prospect of the cell wall breaking of E.ulmoides is proposed in this article.展开更多
Objective:Nonalcoholic fatty liver disease(NAFLD)has become a common chronic liver disease that is harmful to human health.Moreover,there is currently no FDA-approved first-line drug for the treatment of nonalcoholic ...Objective:Nonalcoholic fatty liver disease(NAFLD)has become a common chronic liver disease that is harmful to human health.Moreover,there is currently no FDA-approved first-line drug for the treatment of nonalcoholic steatohepatitis(NASH)or NAFLD.Traditional Chinese medicine(TCM)is widely used to ameliorate liver diseases,such as the traditional ancient recipe called Three Flower Tea(TFT),which consists of double rose(Rosa rugosa),white chrysanthemum(Chrysanthemum morifolium),and Daidaihua(Citrus aurantium).However,the mechanisms of the action of TFT are not clear.Therefore,this study aimed to elucidate the mechanisms of TFT against NAFLD in high-fat diet(HFD)-induced rats.Methods:This study utilized bioinformatics and network pharmacology to establish the active and potential ingredient-target networks of TFT.Furthermore,a protein–protein interaction(PPI)network was constructed,and enrichment analysis was performed to determine the key targets of TFT against NAFLD.Furthermore,an animal experiment was conducted to evaluate the therapeutic effect and confirm the key targets of TFT against NAFLD.Results:A total of 576 NAFLD-related genes were searched in Gene Cards,and under the screening criteria of oral bioavailability(OB)≥30%and drug-likeness(DL)≥0.18,a total of 19 active ingredients and 210 targets were identified in TFT.Network pharmacology analysis suggested that 55 matching targets in PPIs were closely associated with roles for NAFLD treatment.Through the evaluation of network topology parameters,four key central genes,PPARγ,SREBP,AKT,and RELA,were identified.Furthermore,animal experiments indicated that TFT could reduce plasma lipid profiles,hepatic lipid profiles and hepatic fat accumulation,improve liver function,suppress inflammatory factors,and reduce oxidative stress.Through immunoblotting and immunofluorescence analysis,PPARγ,SREBP,AKT,and RELA were confirmed as targets of TFT in HFD-induced rats.Conclusion:In summary,our results indicate that TFT can prevent and treat NAFLD via multiple targets,including lipid accumulation,antioxidation,insulin sensitivity,and inflammation.展开更多
Polysaccharide production from tea flower(TFPS) was carried out using supercritical fluid extraction(SFE).Response surface methodology(RSM),based on a five level,four variable small central composite design,was employ...Polysaccharide production from tea flower(TFPS) was carried out using supercritical fluid extraction(SFE).Response surface methodology(RSM),based on a five level,four variable small central composite design,was employed to obtain the best possible combination of extraction time,pressure,temperature and ethanol content of modifier for maximum production.The optimum conditions were as follows:extraction time of 170 min,pressure of45 MPa,temperature of 75 ℃,and 50% aqueous ethanol solution as modifier.Under these conditions,the experimental yield was 6.56 ± 0.37%,which was similar to the value predicted by the model.Monosaccharide composition of TFPS was fucose,rhamnose,arabinose,xylose,galactose,glucose,mannose,fructose,ribose,galacturonic acid and glucuronic acid in a molar percent of 31.69,0.21,0.49,1.29,35.82,0.97,1.63,18.34,7.88,1.06 and 0.63.Compared to other extraction methods,SFE could achieve higher yield and gain more types of monosaccharide.展开更多
Seasonal variations in environmental conditions can have significant influence on flowering pattern and yield of roses. Evaluation studies were conducted to determine the flowering pattern and yield determinants of tw...Seasonal variations in environmental conditions can have significant influence on flowering pattern and yield of roses. Evaluation studies were conducted to determine the flowering pattern and yield determinants of two Hybrid Tea rose cultivars in response to seasonal variations under rain-fed conditions. The 2 × 2 × 4 factorial experiment arranged in split plot design at three replications was conducted at two seasons (dry and wet) using two cultivars (cv. “Immaculate” and “P.H. Baby”, white and red flowers respectively) supplied with poultry manure (PM) application rates at 5, 10 and 20 t/ha where the unfertilized (0 t/ha) served as control. The result showed that the year two environmental conditions of both experiment I and II significantly improved plant height, number of leaves, number of buds (CNB) and opened flowers (CNF) and flower yield (FYD). “Immaculate” was significantly (p ≤ 0.05) taller with more leaves but thinner girth. Although generally lower in NF/NB, and not different in FYD, the “P.H. Baby” had more flowers (CNB and CNF) in experiment I. Except for no significant difference in FYD and lower NF/NB recorded by all manure rates compared to control in experiment II, plants treated with 5 t/ha PM recorded more flowers (CNB and CNF). Significant first and second order interaction effects (p ≤ 0.05) showed that “Immaculate” had taller height and more leaves but “P.H. Baby” had more CNB and CNF, although both were not different in FYD. In addition, plants supplied with 5 t/ha PM rates were better in plant height, number of leaves, CNB and CNF compared to other manure rates. In conclusion, year two environmental conditions supported better crop growth and yield, “Immaculate” grew better vegetatively but both were not different in floral yield, while the 5 t/ha PM was considered the optimum manure application rate under the rain-fed condition.展开更多
基金Supported by Project of Department of Education of Hunan Province(13CY017)
文摘Eucommia ulmoides male flowers are rich in secondary metabolite,which have anti-tumor,sedative hypnotic,hypotensive,hypolipidemic,anti-fatigue,bacteriostatic,antioxidant and anti-aging effects.The conventional processing of E.ulmoides male flowers leads to the loss of nutrients and active ingredients.In recent years,cell wall breaking technology has been developed and utilized in various fields such as traditional Chinese medicine,good,chemical industry and biology.In order to promote the development of the E.ulmoides industry,the cell wall breaking technology and its characteristics are reviewed,and the application advantages of the cell wall breaking technology in the male flowers of E.ulmoides are discussed,and the prospect of the cell wall breaking of E.ulmoides is proposed in this article.
基金supported by the fund for the National Natural Science Foundation of China(Grant No.81903878)the Natural Science Foundation of Shandong Province,China(Grant No.ZR2019BH049)Major Science and Technology Innovation Project of Shandong Province(Grant No.2019JZZY020612)。
文摘Objective:Nonalcoholic fatty liver disease(NAFLD)has become a common chronic liver disease that is harmful to human health.Moreover,there is currently no FDA-approved first-line drug for the treatment of nonalcoholic steatohepatitis(NASH)or NAFLD.Traditional Chinese medicine(TCM)is widely used to ameliorate liver diseases,such as the traditional ancient recipe called Three Flower Tea(TFT),which consists of double rose(Rosa rugosa),white chrysanthemum(Chrysanthemum morifolium),and Daidaihua(Citrus aurantium).However,the mechanisms of the action of TFT are not clear.Therefore,this study aimed to elucidate the mechanisms of TFT against NAFLD in high-fat diet(HFD)-induced rats.Methods:This study utilized bioinformatics and network pharmacology to establish the active and potential ingredient-target networks of TFT.Furthermore,a protein–protein interaction(PPI)network was constructed,and enrichment analysis was performed to determine the key targets of TFT against NAFLD.Furthermore,an animal experiment was conducted to evaluate the therapeutic effect and confirm the key targets of TFT against NAFLD.Results:A total of 576 NAFLD-related genes were searched in Gene Cards,and under the screening criteria of oral bioavailability(OB)≥30%and drug-likeness(DL)≥0.18,a total of 19 active ingredients and 210 targets were identified in TFT.Network pharmacology analysis suggested that 55 matching targets in PPIs were closely associated with roles for NAFLD treatment.Through the evaluation of network topology parameters,four key central genes,PPARγ,SREBP,AKT,and RELA,were identified.Furthermore,animal experiments indicated that TFT could reduce plasma lipid profiles,hepatic lipid profiles and hepatic fat accumulation,improve liver function,suppress inflammatory factors,and reduce oxidative stress.Through immunoblotting and immunofluorescence analysis,PPARγ,SREBP,AKT,and RELA were confirmed as targets of TFT in HFD-induced rats.Conclusion:In summary,our results indicate that TFT can prevent and treat NAFLD via multiple targets,including lipid accumulation,antioxidation,insulin sensitivity,and inflammation.
基金supported by a key research grant "The application and Utilization of Tea (Camellia sinensis) Flowers" from the 2013 Zhejiang Province
文摘Polysaccharide production from tea flower(TFPS) was carried out using supercritical fluid extraction(SFE).Response surface methodology(RSM),based on a five level,four variable small central composite design,was employed to obtain the best possible combination of extraction time,pressure,temperature and ethanol content of modifier for maximum production.The optimum conditions were as follows:extraction time of 170 min,pressure of45 MPa,temperature of 75 ℃,and 50% aqueous ethanol solution as modifier.Under these conditions,the experimental yield was 6.56 ± 0.37%,which was similar to the value predicted by the model.Monosaccharide composition of TFPS was fucose,rhamnose,arabinose,xylose,galactose,glucose,mannose,fructose,ribose,galacturonic acid and glucuronic acid in a molar percent of 31.69,0.21,0.49,1.29,35.82,0.97,1.63,18.34,7.88,1.06 and 0.63.Compared to other extraction methods,SFE could achieve higher yield and gain more types of monosaccharide.
文摘Seasonal variations in environmental conditions can have significant influence on flowering pattern and yield of roses. Evaluation studies were conducted to determine the flowering pattern and yield determinants of two Hybrid Tea rose cultivars in response to seasonal variations under rain-fed conditions. The 2 × 2 × 4 factorial experiment arranged in split plot design at three replications was conducted at two seasons (dry and wet) using two cultivars (cv. “Immaculate” and “P.H. Baby”, white and red flowers respectively) supplied with poultry manure (PM) application rates at 5, 10 and 20 t/ha where the unfertilized (0 t/ha) served as control. The result showed that the year two environmental conditions of both experiment I and II significantly improved plant height, number of leaves, number of buds (CNB) and opened flowers (CNF) and flower yield (FYD). “Immaculate” was significantly (p ≤ 0.05) taller with more leaves but thinner girth. Although generally lower in NF/NB, and not different in FYD, the “P.H. Baby” had more flowers (CNB and CNF) in experiment I. Except for no significant difference in FYD and lower NF/NB recorded by all manure rates compared to control in experiment II, plants treated with 5 t/ha PM recorded more flowers (CNB and CNF). Significant first and second order interaction effects (p ≤ 0.05) showed that “Immaculate” had taller height and more leaves but “P.H. Baby” had more CNB and CNF, although both were not different in FYD. In addition, plants supplied with 5 t/ha PM rates were better in plant height, number of leaves, CNB and CNF compared to other manure rates. In conclusion, year two environmental conditions supported better crop growth and yield, “Immaculate” grew better vegetatively but both were not different in floral yield, while the 5 t/ha PM was considered the optimum manure application rate under the rain-fed condition.
