Interspecific alien addition lines have played significant roles in gene mapping, intergenomic gene transfer and chromosomal homoeological identification between closely related species. Selection of alien addition li...Interspecific alien addition lines have played significant roles in gene mapping, intergenomic gene transfer and chromosomal homoeological identification between closely related species. Selection of alien addition lines was conducted by karyotype analysis and morphological observation with the reference of parents. Triploid interspecies hybrid （AAC, 2n = 3x = 29） was obtained from Brassica campestris ssp. chinensis var. parachinensis Qinglu 9601 （tetraploid, AAAA, 2n = 4x = 40） x B. oleracea var. alboglabra Baihua 9705 （diploid, CC, 2n = 2x = 18） by immature hybrid embryo culture in vitro. Five different alien monosomic addition lines （AA ＋ C2, AA ＋ C3, AA ＋ C4, AA ＋ C6, AA ＋ C7） were obtained from the backcross progenies of AAC x AA. Each alien monosomic addition line has some specific morphological characters. It is feasible to obtain alien addition lines from the progenies of AAC × AA by karyotype analysis and morphological observation based on the reference of parents展开更多
WRKY is a plant-specific transcription factor(TF) involved in the regulation of many biological processes; however, its role in leaf senescence of leafy vegetables remains unknown. In the present work, a WRKY TF, term...WRKY is a plant-specific transcription factor(TF) involved in the regulation of many biological processes; however, its role in leaf senescence of leafy vegetables remains unknown. In the present work, a WRKY TF, termed Br WRKY75 was isolated from Chinese flowering cabbage [Brassica rapa L. ssp. chinensis(L.) Mokino var. utilis Tsen et Lee]. Analysis of deduced amino acid sequence and the phylogenetic tree showed that Br WRKY75 has high homology with WRKY75 from Brassica oleracea and Arabidopsis thaliana, and belongs to the II c sub-group. Sub-cellular localization and transcriptional activity analysis revealed that Br WRKY75 is a nuclear protein with transcriptional repression activity, and was up-regulated during leaf senescence. Electrophoretic mobility shift assay confirmed that Br WRKY75 directly bound to the W-box(TTGAC) cis-element. Collectively,these results provide a basis for further investigation of the transcriptional regulation of Chinese flowering cabbage leaf senescence.展开更多
基金funded by the Natural Science Foundation of Hebei Province,China(C2006000450).
文摘Interspecific alien addition lines have played significant roles in gene mapping, intergenomic gene transfer and chromosomal homoeological identification between closely related species. Selection of alien addition lines was conducted by karyotype analysis and morphological observation with the reference of parents. Triploid interspecies hybrid （AAC, 2n = 3x = 29） was obtained from Brassica campestris ssp. chinensis var. parachinensis Qinglu 9601 （tetraploid, AAAA, 2n = 4x = 40） x B. oleracea var. alboglabra Baihua 9705 （diploid, CC, 2n = 2x = 18） by immature hybrid embryo culture in vitro. Five different alien monosomic addition lines （AA ＋ C2, AA ＋ C3, AA ＋ C4, AA ＋ C6, AA ＋ C7） were obtained from the backcross progenies of AAC x AA. Each alien monosomic addition line has some specific morphological characters. It is feasible to obtain alien addition lines from the progenies of AAC × AA by karyotype analysis and morphological observation based on the reference of parents
基金supported by the ‘The Twelfth Five-Year Plan’ National Science and Technology Support Program (2013BAD19B07)
文摘WRKY is a plant-specific transcription factor(TF) involved in the regulation of many biological processes; however, its role in leaf senescence of leafy vegetables remains unknown. In the present work, a WRKY TF, termed Br WRKY75 was isolated from Chinese flowering cabbage [Brassica rapa L. ssp. chinensis(L.) Mokino var. utilis Tsen et Lee]. Analysis of deduced amino acid sequence and the phylogenetic tree showed that Br WRKY75 has high homology with WRKY75 from Brassica oleracea and Arabidopsis thaliana, and belongs to the II c sub-group. Sub-cellular localization and transcriptional activity analysis revealed that Br WRKY75 is a nuclear protein with transcriptional repression activity, and was up-regulated during leaf senescence. Electrophoretic mobility shift assay confirmed that Br WRKY75 directly bound to the W-box(TTGAC) cis-element. Collectively,these results provide a basis for further investigation of the transcriptional regulation of Chinese flowering cabbage leaf senescence.
