The steroid hormone brassinosteroid (BR) plays a broad role in plant growth and development. As the retarded growth in BR-insensitive and BR-deficient mutants causes a strong delay in days to flowering, BR signaling...The steroid hormone brassinosteroid (BR) plays a broad role in plant growth and development. As the retarded growth in BR-insensitive and BR-deficient mutants causes a strong delay in days to flowering, BR signaling has been thought to promote the floral transition inArabidopsis. In this study, using a developmental measure of flowering time, we show that BR signaling inhibits the floral transition and promotes vegetative growth in the Arabidopsis accessions Columbia and Enkheim-2. We found that BR signaling promotes the expression of the potent floral repressor FLOWERING LOCUS C (FLC) and three FLC homologs to inhibit flow- ering. In the presence of BR, the transcription factor BRASSINAZOLE-RESISTANT1 (BZR1), together with BES1 -INTERACTING MYC-like proteins (BIMs), specifically binds a BR- responsive element in the first intron of FLC and further recruits a histone 3 lysine 27 (H3K27) demethylase to downregulate levels of the repressive H3K27 trimethylation mark and thus antagonize Polycomb silencing at FLC, leading to its activation. Taken together, our findings demonstrate that BR signaling inhibits the floral transition inArabidopsis by a novel molecular mechanism in which BR signals are transduced into FLC activation and consequent floral repression.展开更多
【目的】探明当归(Angelica sinensis)春化作用相关SOSEKI(SOK)基因的生物学功能,分析其对春化作用和冷冻温度的表达响应。【方法】基于当归种苗春化作用(0℃)和冷冻贮藏(-3℃)后的全长转录组测序,挖掘春化作用基因FLOWERING LOCUS C(FL...【目的】探明当归(Angelica sinensis)春化作用相关SOSEKI(SOK)基因的生物学功能,分析其对春化作用和冷冻温度的表达响应。【方法】基于当归种苗春化作用(0℃)和冷冻贮藏(-3℃)后的全长转录组测序,挖掘春化作用基因FLOWERING LOCUS C(FLC),利用在线生物信息学工具分析其生物学功能,并对表达模式进行qRT-PCR验证。【结果】从当归全长转录组中挖掘到一个含1005个碱基的AsSOK基因,包括FLC及其侧翼基因UPSTREAM OF FLC(UFC)和DOWNSTREAM OF FLC(DFC),亚细胞定位于细胞核;保守基序和系统进化树分析显示,该基因编码的蛋白质含有4个保守基序(motif-2、-4、-5和-6),与黄胡萝卜(Daucus carota subsp.Sativus)中的LOC Dc108213152关系最近,具有相似的结构和位置。基因表达检测发现,AsSOK基因在种苗春化作用过程中表达量显著下降,而在冷冻贮藏过程中显著增加。【结论】第一次挖掘到并全面分析了当归春化作用相关基因AsSOK的生物学功能,为利用基因编辑AsSOK调控当归抽薹开花等研究提了供理论基础。展开更多
The developmental transition to flowering in many plants is timed by changing seasons,which enables plants to flower at a season that is favorable for seed production.Many plants grown at high latitudes perceive the s...The developmental transition to flowering in many plants is timed by changing seasons,which enables plants to flower at a season that is favorable for seed production.Many plants grown at high latitudes perceive the seasonal cues of changing day length and/or winter cold(prolonged cold exposure),to regulate the expression of flowering-regulatory genes through the photoperiod pathway and/or vernalization pathway,and thus align flowering with a particular season.Recent studies in the model flowering plant Arabidopsis thaliana have revealed that diverse transcription factors engage various chromatin modifiers to regulate several key flowering-regulatory genes including FLOWERING LOCUS C(FLC)and FLOWERING LOCUS T(FT)in response to seasonal signals.Here,we summarize the current understanding of molecular and chromatin-regulatory or epigenetic mechanisms underlying the vernalization response and photoperiodic control of flowering in Arabidopsis.Moreover,the conservation and divergence of regulatory mechanisms for seasonal flowering in crops and other plants are briefly discussed.展开更多
基金This work was supported in part by funding from the National Natural Science Foundation of China (grant number 31721001), the National Key Research and Development Program of China (2017YFA0503803), and the Chinese Academy of Sciences (XDPB0404).
文摘The steroid hormone brassinosteroid (BR) plays a broad role in plant growth and development. As the retarded growth in BR-insensitive and BR-deficient mutants causes a strong delay in days to flowering, BR signaling has been thought to promote the floral transition inArabidopsis. In this study, using a developmental measure of flowering time, we show that BR signaling inhibits the floral transition and promotes vegetative growth in the Arabidopsis accessions Columbia and Enkheim-2. We found that BR signaling promotes the expression of the potent floral repressor FLOWERING LOCUS C (FLC) and three FLC homologs to inhibit flow- ering. In the presence of BR, the transcription factor BRASSINAZOLE-RESISTANT1 (BZR1), together with BES1 -INTERACTING MYC-like proteins (BIMs), specifically binds a BR- responsive element in the first intron of FLC and further recruits a histone 3 lysine 27 (H3K27) demethylase to downregulate levels of the repressive H3K27 trimethylation mark and thus antagonize Polycomb silencing at FLC, leading to its activation. Taken together, our findings demonstrate that BR signaling inhibits the floral transition inArabidopsis by a novel molecular mechanism in which BR signals are transduced into FLC activation and consequent floral repression.
文摘【目的】探明当归(Angelica sinensis)春化作用相关SOSEKI(SOK)基因的生物学功能,分析其对春化作用和冷冻温度的表达响应。【方法】基于当归种苗春化作用(0℃)和冷冻贮藏(-3℃)后的全长转录组测序,挖掘春化作用基因FLOWERING LOCUS C(FLC),利用在线生物信息学工具分析其生物学功能,并对表达模式进行qRT-PCR验证。【结果】从当归全长转录组中挖掘到一个含1005个碱基的AsSOK基因,包括FLC及其侧翼基因UPSTREAM OF FLC(UFC)和DOWNSTREAM OF FLC(DFC),亚细胞定位于细胞核;保守基序和系统进化树分析显示,该基因编码的蛋白质含有4个保守基序(motif-2、-4、-5和-6),与黄胡萝卜(Daucus carota subsp.Sativus)中的LOC Dc108213152关系最近,具有相似的结构和位置。基因表达检测发现,AsSOK基因在种苗春化作用过程中表达量显著下降,而在冷冻贮藏过程中显著增加。【结论】第一次挖掘到并全面分析了当归春化作用相关基因AsSOK的生物学功能,为利用基因编辑AsSOK调控当归抽薹开花等研究提了供理论基础。
基金Research in the Plant Environmental Epigenetics laboratory is supported in part by the National Natural Science Foundation of China(31830049)the National Key Research and Development Program of China(2017YFA0503803)the Chinese Academy of Sciences(XDB27030202)。
文摘The developmental transition to flowering in many plants is timed by changing seasons,which enables plants to flower at a season that is favorable for seed production.Many plants grown at high latitudes perceive the seasonal cues of changing day length and/or winter cold(prolonged cold exposure),to regulate the expression of flowering-regulatory genes through the photoperiod pathway and/or vernalization pathway,and thus align flowering with a particular season.Recent studies in the model flowering plant Arabidopsis thaliana have revealed that diverse transcription factors engage various chromatin modifiers to regulate several key flowering-regulatory genes including FLOWERING LOCUS C(FLC)and FLOWERING LOCUS T(FT)in response to seasonal signals.Here,we summarize the current understanding of molecular and chromatin-regulatory or epigenetic mechanisms underlying the vernalization response and photoperiodic control of flowering in Arabidopsis.Moreover,the conservation and divergence of regulatory mechanisms for seasonal flowering in crops and other plants are briefly discussed.