Studying on the genetic diversity and genetic relationship of flowering cherry cultivars is extremely important for germplasm conservation, cultivar identification and breeding. Flowering cherry is widely cultivated a...Studying on the genetic diversity and genetic relationship of flowering cherry cultivars is extremely important for germplasm conservation, cultivar identification and breeding. Flowering cherry is widely cultivated as an important woody ornamental plant in worldwide, especially Japan, China. However, owning to the morphological similarity, many cultivars are distinguished hardly in non-flowering season. Here, we evaluated the genetic diversity and genetic relationship of 40 flowering cherry cultivars, which are mainly cultivated in China. We selected 13 polymorphicprimers to amplify to allele fragments with fluorescent-labeled capillary electrophoresis technology. The population structure analysis results show that these cultivars could be divided into 4 subpopulations. At the population level, N<sub>a</sub> and N<sub>e</sub> were 6.062, 4.326, respectively. H<sub>o</sub> and H<sub>e</sub> were 0.458 and 0.670, respectively. The Shannon’s information index (I) was 1.417. The Pop3, which originated from P. serrulata, had the highest H<sub>o</sub>, H<sub>e</sub>, and I among the 4 subpopulations. AMOVA showed that only 4% of genetic variation came from populations, the 39% variation came from individuals and 57% (p < 0.05) came from intra-individuals. 5 polymorphic SSR primers were selected to construct molecular ID code system of these cultivars. This analysis on the genetic diversity and relationship of the 40 flowering cherry cultivars will help to insight into the genetic background, relationship of these flowering cherry cultivars and promote to identify similar cultivars.展开更多
[Objectives] This study was conducted to analyze the current status of soil quality in Chongqing and provide scientific data for the rejuvenation of flowering cherry in the botanical park of the city. [Methods] With t...[Objectives] This study was conducted to analyze the current status of soil quality in Chongqing and provide scientific data for the rejuvenation of flowering cherry in the botanical park of the city. [Methods] With the flowering cherry soil in Chongqing Botanical Park as the research object, the typical soil in this area was investigated and improved. [Results] After applying soil conditioners, the pH value of the surface soil decreased from 7.32 to 6.34, and the pH value of the bottom soil decreased from 7.52 to 6.77;and the surface soil organic matter increased from 35.2 to 54.1 g/kg, and the bottom soil organic matter increased from 15.7 to 26.3 g/kg. [Conclusions] The soil has strong buffering capacity, and it is difficult to achieve rapid adjustment of soil pH and organic matter in actual work. Soil improvement needs to be carried out step by step. After targeted improvement, further maintenance is required in the later stage.展开更多
文摘Studying on the genetic diversity and genetic relationship of flowering cherry cultivars is extremely important for germplasm conservation, cultivar identification and breeding. Flowering cherry is widely cultivated as an important woody ornamental plant in worldwide, especially Japan, China. However, owning to the morphological similarity, many cultivars are distinguished hardly in non-flowering season. Here, we evaluated the genetic diversity and genetic relationship of 40 flowering cherry cultivars, which are mainly cultivated in China. We selected 13 polymorphicprimers to amplify to allele fragments with fluorescent-labeled capillary electrophoresis technology. The population structure analysis results show that these cultivars could be divided into 4 subpopulations. At the population level, N<sub>a</sub> and N<sub>e</sub> were 6.062, 4.326, respectively. H<sub>o</sub> and H<sub>e</sub> were 0.458 and 0.670, respectively. The Shannon’s information index (I) was 1.417. The Pop3, which originated from P. serrulata, had the highest H<sub>o</sub>, H<sub>e</sub>, and I among the 4 subpopulations. AMOVA showed that only 4% of genetic variation came from populations, the 39% variation came from individuals and 57% (p < 0.05) came from intra-individuals. 5 polymorphic SSR primers were selected to construct molecular ID code system of these cultivars. This analysis on the genetic diversity and relationship of the 40 flowering cherry cultivars will help to insight into the genetic background, relationship of these flowering cherry cultivars and promote to identify similar cultivars.
基金Supported by Chongqing City Administration Bureau Science and Technology Planning Project(YKZ 2017 05)Science and Technology Platform and Base Construction of Chongqing City(cstc2014gjhz20001)Chongqing Science and Technology Planning Project(cstc2011pt-gc80019)
文摘[Objectives] This study was conducted to analyze the current status of soil quality in Chongqing and provide scientific data for the rejuvenation of flowering cherry in the botanical park of the city. [Methods] With the flowering cherry soil in Chongqing Botanical Park as the research object, the typical soil in this area was investigated and improved. [Results] After applying soil conditioners, the pH value of the surface soil decreased from 7.32 to 6.34, and the pH value of the bottom soil decreased from 7.52 to 6.77;and the surface soil organic matter increased from 35.2 to 54.1 g/kg, and the bottom soil organic matter increased from 15.7 to 26.3 g/kg. [Conclusions] The soil has strong buffering capacity, and it is difficult to achieve rapid adjustment of soil pH and organic matter in actual work. Soil improvement needs to be carried out step by step. After targeted improvement, further maintenance is required in the later stage.
