Gastric ulcer is a widespread disease caused by various etiologies.Dendrobium offi cinale flowers exert several health benefits owing to their rich flavonoid content.In this study,protective effects and possible actio...Gastric ulcer is a widespread disease caused by various etiologies.Dendrobium offi cinale flowers exert several health benefits owing to their rich flavonoid content.In this study,protective effects and possible action mechanisms of D.offi cinale flowers’flavonoid enriched extract(DOFF)were assessed against gastric ulcer.The result of sodium nitrite-aluminum nitrate colorimetry showed that 52.34%of the total extractive was flavonoid,and ultra-high performance liquid chromatography time of flight mass spectrometer(UPLC-QTOF/MS)revealed the presence of 28 components in DOFF of which 14 belonged to flavonoids.In addition,in vivo assay revealed DOFF potential in reducing the formation of ethanol-induced gastric mucosal lesions,with drop-in ulcer index from 64.33±8.76 to 32.00±4.47.Similar results were revealed in human gastric mucosal epithelia cells,with cells viability to increase from 27.2%to 61.6%post DOFF administration.To analyzed the protect effect of DOFF,we used Western blotting and immunofluorometric assay to revealed the expression levels of key proteins in cell pathways.The results showed that DOFF(320μg/mL)could increase the level of oxidation marker protein(HO-1),apoptosis regulatory protein(Bcl-2)and autophagy marker(LC3β)by 50.84%,43.85%,and 59.21%compared with ethanol-treated group respectively.Further analyzed of the mitochondrial activity and apoptosis pathway,we found that DOFF appeared to mitigate against ethanolinduced gastric mucosal injury via AMPK/mTOR/ULK1 and PI3K/AKT autophagy signaling pathways.展开更多
Poetry,as a crucial form of literary expression,often employs metaphor as a common rhetorical device.According to the perspective of cognitive metaphor theory,metaphor transcends its traditional linguistic boundaries ...Poetry,as a crucial form of literary expression,often employs metaphor as a common rhetorical device.According to the perspective of cognitive metaphor theory,metaphor transcends its traditional linguistic boundaries and is recognized as a profound cognitive mechanism,manifesting as a broader cognitive phenomenon.This article is based on metaphorical examples of“flowers”in Chinese and English poetry,carefully selecting representative cases for in-depth analysis.The aim is to compare the imagery of“flowers”in Chinese and English poetry,observe their similarities and differences,thereby fostering a better understanding of poetry in both languages.Through this study,we not only delve into the intricacies of metaphor within poetry but also shed light on the distinct interpretations of the symbol of“flowers”in different cultural contexts,expanding our appreciation for the cultural diversity inherent in poetry.展开更多
In urban road greening,the planting of ground cover flowers is essential,and herbaceous flowers are an important part.Through the investigation on the diversity of herbaceous flowers in urban road green spaces of Hefe...In urban road greening,the planting of ground cover flowers is essential,and herbaceous flowers are an important part.Through the investigation on the diversity of herbaceous flowers in urban road green spaces of Hefei City,the types,colors,application frequency and planting of herbaceous flowers in urban road green spaces were statistically analyzed,and the application forms of herbaceous flowers in the road greening of Hefei City were studied.The characteristics and problems of herbaceous flowers in the road greening of Hefei City were found,and some development suggestions were put forward.展开更多
Spatial narrative,as a unique literary narrative technique,focuses on advancing the plot and shaping characters through meticulous portrayal of space,providing readers with rich visual and perceptual experiences.This ...Spatial narrative,as a unique literary narrative technique,focuses on advancing the plot and shaping characters through meticulous portrayal of space,providing readers with rich visual and perceptual experiences.This paper examines the spatial narrative utilized in Jin Yucheng’s novel,Flowers of Shanghai.Through an in-depth analysis of the spatial narrative techniques in the novel,this paper asserts that such an exploration has far-reaching consequences in broadening research perspectives and fostering interdisciplinary collaborations between literature and geography.Furthermore,the paper explores the unique aspects of narrative perspective,narrative structure,and narrative time in Flowers of Shanghai,and how these elements perfectly combine with spatial narrative to create a fascinating literary world for readers.The aim of this study is to deeply explore the artistic value and literary charm of the novel through a comprehensive interpretation of the spatial narrative in Flowers of Shanghai.展开更多
The active ingredients of Camellia japonica flowers(CJF)at high and low altitudes,as well as their skin care efficacy were compared.The dried red CJF at high and low altitudes were ultrasonically extracted with 70%eth...The active ingredients of Camellia japonica flowers(CJF)at high and low altitudes,as well as their skin care efficacy were compared.The dried red CJF at high and low altitudes were ultrasonically extracted with 70%ethanol,and these extracts were concentrated and then diluted to a constant volume.The content of total flavonoids,total polyphenols and total proteins was tested and analyzed.In addition,DPPH free radical scavenging,inhibition of the formation of advanced glycation end products,and inhibitory activity against elastase was measured to compare their skin care efficacy in anti-oxidation,anti-glycation,anti-wrinkling and firming.The results showed that,based on the dry weight of CJF,the content of total flavonoids and total proteins of the CJF at high altitude was approximately 1.5 times of the CJF at low altitude,while the content of total polyphenols was approximately 2.4 times that of the CJF at low altitude.The skin care efficacy of CJF at high altitude was significantly better than that of the CJF at low altitude.This work could provide theoretical basis for the selection and application of Camellia japonica flowers in the field of cosmetics.展开更多
Flower plants are popular all over the world and important sources of ornamental plants,bioactive molecules and nutrients.Flowers have a wide range of biological activities and beneficial pharmacological effects.Flowe...Flower plants are popular all over the world and important sources of ornamental plants,bioactive molecules and nutrients.Flowers have a wide range of biological activities and beneficial pharmacological effects.Flowers and their active ingredients are becoming more and more popular in the preparation of food,drugs and industrial products.This paper summarizes the active ingredients,pharmacological activities and applications in the pharmaceutical and food industries of flower plants in recent years.In addition,the possible molecular mechanism of pharmacological effects of flower plants were also discussed.302 active constituents from 55 species of flower plants were summarized,including flavonoids(115),terpenoids(90),phenylpropanoids(20),alkaloids(13),organic acids(27)and others(37).The pharmacological effects of flower plants are very extensive,mainly including antioxidant,anti-inflammatory,anti-tumor,anti-virus,and hypoglycemic.The mechanisms of anti-inflammatory,anti-tumor and hypoglycemic activities present the characteristics of multi-way and multi-target.Because of its rich nutrients,bioactive ingredients and plant essential oils,and its wide sources,flower plants are widely used in food,beverage,cosmetics and drug research.Flower plants also play an important role in pharmaceutical industry,food industry and other fields.展开更多
Low temperature is among the most restrictive factors to limit the yield and distribution of pear. Pyrus hopeiensis is a valuable wild resource.PCA showed that P. hopeiensis had strong cold resistance. In this study, ...Low temperature is among the most restrictive factors to limit the yield and distribution of pear. Pyrus hopeiensis is a valuable wild resource.PCA showed that P. hopeiensis had strong cold resistance. In this study, the mRNA and metabolome sequencing of P. hopeiensis flower organs exposed to different low temperatures were performed to identify changes of genes and metabolites in response to low-temperature stress. A total of 4 851 differentially expressed genes(DEGs) were identified. Trend analysis showed that these DEGs were significantly enriched in profiles 19, 18, 7, 14, 1, 4 and 11. And the KEGG enrichment analysis showed that the DEGs in profile 18 were significantly enriched in flavone and flavonol biosynthesis. Besides, the expressed trends as well as GO and KEGG functional enrichment analyses of DEGs under cold and freezing stress showed significantly difference. Analyses of flavonoid-related pathways indicated that flavonoid structural genes had undergone significant changes. Correlation analysis showed that b HLH and MYB TFs may affect flavonoid biosynthesis by regulating structural gene expression. And PhMYB308 and PhMYB330 were likely candidate repressors of flavonoid biosynthesis by binding to a specific site in bHLH proteins. In total, 92 differentially accumulated metabolites(DAMs) were identified in P. hopeiensis flowers including 12 flavonoids. WGCNA results showed that coral 1, pink and brown 4 modules were closely associated with flavonoids and 11 MYBs and 15 bHLHs among the three modules may activate or inhibit the expression of 23 structural genes of flavonoid biosynthesis. Taken together, the results of this study provided a theoretical basis for further exploration of the molecular mechanisms of flavonoid biosynthesis and cold resistance of P. hopeiensis flower organs and our findings laid a foundation for further molecular breeding in cold-resistant pear varieties.展开更多
The juvenile-to-adult phase change with first flowering as the indicator plays a crucial role in the lifecycle of fruit trees. However, the molecular mechanisms underlying phase change in fruit trees remain largely un...The juvenile-to-adult phase change with first flowering as the indicator plays a crucial role in the lifecycle of fruit trees. However, the molecular mechanisms underlying phase change in fruit trees remain largely unknown. Shikimic acid (ShA) pathway is a main metabolic pathway closely related to the synthesis of hormones and many important secondary metabolites participating in plant phase change. So,whether ShA regulates phase change in plants is worth clarifying. Here, the distinct morphological characteristics and the underlying mechanisms of phase change in jujube (Ziziphus jujuba Mill.), an important fruit tree native to China with nutritious fruit and outstanding tolerance abiotic stresses, were clarified. A combined transcriptome and metabolome analysis found that ShA is positively involved in jujube(Yuhong’×Xing 16’) phase change. The genes in the upstream of ShA synthesis pathway (ZjDAHPS, ZjDHQS and ZjSDH), the contents of ShA and the downstream secondary metabolites like phenols were significantly upregulated in the phase change period. Further, the treatment of spraying exogenous ShA verified that ShA at a very low concentration (60 mg·L^(-1)) can substantially speed up the phase change and flowering of jujube and other tested plants including Arabidopsis, tomato and wheat. The exogenous ShA (60 mg·L^(-1)) treatment in jujube seedlings could increase the accumulation of endogenous ShA, enhance leaf photosynthesis and the synthesis of phenols especially flavonoids and phenolic acids, and promote the expression of genes (ZjCOs, ZjNFYs and ZjPHYs) involved in flowering pathway. Basing on above results, we put forward a propose for the underlying mechanism of ShA regulating phase change, and a hypothesis that ShA could be considered a phytohormone-like substance because it is endogenous, ubiquitous, movable and highly efficient at very low concentrations. This study highlights the critical role of ShA in plant phase change and its phytohormone-like properties.展开更多
Nymphaea is a key genus of the ANA grade(Amborellales,Nymphaeales,and Austrobaileyales)of basal flowering plants,which serve as a key model to study the early evolution of floral traits.In this study,we comprehensivel...Nymphaea is a key genus of the ANA grade(Amborellales,Nymphaeales,and Austrobaileyales)of basal flowering plants,which serve as a key model to study the early evolution of floral traits.In this study,we comprehensively investigated the emission,biosynthesis,and biological function of the floral scent in a night-blossoming waterlily Nymphaea prolifera.The headspace volatile collection combined with GC-MS analysis showed that the floral scent of N.prolifera is predominately comprised by methylated benzenoids including anisole,veratrole,guaiacol,and methoxyanisole.Moreover,the emission of these floral benzenoids in N.prolifera exhibited temporal and spatial pattern with circadian rhythm and tissue specificity.By creating and mining transcriptomes of N.prolifera flowers,12 oxygen methyltransferases(NpOMTs)were functionally identified.By in vitro enzymatic assay,NpOMT3,6,and 7 could produce anisole and NpOMT5,7,9,produce guaiacol,whereas NpOMT3,6,9,11 catalyzed the formation of veratrole.Methoxyanisole was identified as the universal product of all NpOMTs.Expression patterns of NpOMTs provided implication for their roles in the production of the respective benzenoids.Phylogenetic analysis of OMTs suggested a Nymphaea-specific expansion of the OMT family,indicating the evolution of lineage-specific functions.In bioassays,anisole,veratrole,and guaiacol in the floral benzenoids were revealed to play the critical role in repelling waterlily aphids.Overall,this study indicates that the basal flowering plant N.prolifera has evolved a diversity and complexity of OMT genes for the biosynthesis of methylated benzenoids that can repel insects from feeding the flowers.These findings provide new insights into the evolutional mechanism and ecological significance of the floral scent from early-diverged flowering plants.展开更多
Early flowering promotes early maturity, production, and the capacity to counteract biotic and abiotic stresses, making it an important agronomic trait in zucchini. The present study demonstrated that the zucchini inb...Early flowering promotes early maturity, production, and the capacity to counteract biotic and abiotic stresses, making it an important agronomic trait in zucchini. The present study demonstrated that the zucchini inbred line ‘19’ consistently flowered early, taking significantly fewer days to bloom the first female flower(DFF) than the inbred line ‘113’. Genetic analysis revealed that DFF, an inheritable quantitative trait, is controlled by multiple genes. Based on the strategy of quantitative trait locus(QTL) sequencing(QTL-seq) combined with linkage analysis, three QTLs for DFF were identified on chromosomes 4, 11, and 20. This study used additional F2 populations grown under different environmental conditions for QTL mapping analysis of DFF with insertion/deletion(InDel) markers to validate these results. Using the composite interval mapping(CIM) method of R/qtl software, we only identified one major locus under all environmental conditions, located in a 117-kb candidate region on chromosome 20. Based on gene annotation, gene sequence alignment, and qRT-PCR analysis, we found that the Cp4.1LG20g08050 gene encoding a RING finger protein may be a candidate gene for the opposite regulation of early flowering in zucchini. In summary, these results lay a foundation for a better understanding of early flowering and improving early flowering-based breeding strategies in zucchini.展开更多
This review summarizes the main chemical composition of Aloe vera flowers and introduces their antioxidant,anti-aging,skin whitening,skin moisturizing,anti-skin irritation,and anti-skin allergy effects.Suggestions for...This review summarizes the main chemical composition of Aloe vera flowers and introduces their antioxidant,anti-aging,skin whitening,skin moisturizing,anti-skin irritation,and anti-skin allergy effects.Suggestions for the application of Aloe v.flowers in cosmetics are provided.展开更多
The article presents the biology of flowering and the daily dynamics of flowering of two species from the Lamiaceae family: ph. anisochila va ph. sogdiana of the distribution out in the Nuratau Mountains range.
Soybean(Glycine max)is a short-day crop whose flowering time is regulated by photoperiod.The longjuvenile trait extends its vegetative phase and increases yield under short-day conditions.Natural variation in J,the ma...Soybean(Glycine max)is a short-day crop whose flowering time is regulated by photoperiod.The longjuvenile trait extends its vegetative phase and increases yield under short-day conditions.Natural variation in J,the major locus controlling this trait,modulates flowering time.We report that the three J-family genes influence soybean flowering time,with the triple mutant Guangzhou Mammoth-2 flowering late under short days by inhibiting transcription of E1-family genes.J-family genes offer promising allelic combinations for breeding.展开更多
Properly regulated flowering time is pivotal for successful plant reproduction.The floral transition from vegetative growth to reproductive growth is regulated by a complex gene regulatory network that integrates envi...Properly regulated flowering time is pivotal for successful plant reproduction.The floral transition from vegetative growth to reproductive growth is regulated by a complex gene regulatory network that integrates environmental signals and internal conditions to ensure that flowering takes place under favorable conditions.Brassica rapa is a diploid Cruciferae species that includes several varieties that are cultivated as vegetable or oil crops.Flowering time is one of the most important agricultural traits of B.rapa crops because of its influence on yield and quality.The transition to flowering in B.rapa is regulated by several environmental and developmental cues,which are perceived by several signaling pathways,including the vernalization pathway,the autonomous pathway,the circadian clock,the thermosensory pathway,and gibberellin(GA)signaling.These signals are integrated to control the expression of floral integrators BrFTs and BrSOC1s to regulate flowering.In this review,we summarized current research advances on the molecular mechanisms that govern flowering time regulation in B.rapa and compare this to what is known in Arabidopsis.展开更多
Basic helix-loop-helix(bHLH)transcription factor gene family in plants controls various growth and development aspects;however,the actual roles of these genes in flowering plants are not well known.In this study,a nov...Basic helix-loop-helix(bHLH)transcription factor gene family in plants controls various growth and development aspects;however,the actual roles of these genes in flowering plants are not well known.In this study,a novel bHLH protein CmbHLH110 was found to interact with CmERF110 by in vitro and in vivo experiments,a chrysanthemum ERF110 homolog that acts as a positive flowering regulator.In addition,CmbHLH110 was also found to regulate the flowering of chrysanthemums,overexpression of CmbHLH110 causes chrysanthemums to flower earlier,and suppressed CmbHLH110 leads to delayed flowering.Furthermore,the loss-of-function Arabidopsis mutant of its homologue PERICYCLE FACTOR TYPE-A 5(PFA5)had a noticeable late flowering phenotype,and CmbHLH110 completely complemented the late flowering phenotype of the pfa5 mutant,whereas heterologous overexpression of CmbHLH110 in Arabidopsis Col-0 caused early flowering.Transcriptome sequencing revealed significant differential expression of flowering-related and circadian clock-related genes in transgenic chrysanthemum.Therefore,we concluded that CmbHLH110,as a novel flowering regulator,could interact with CmERF110 to regulate flowering in chrysanthemum.展开更多
Flowering time is important for adaptation of soybean(Glycine max)to different environments.Here,we conducted a genome-wide association study of flowering time using a panel of 1490 cultivated soybean accessions.We id...Flowering time is important for adaptation of soybean(Glycine max)to different environments.Here,we conducted a genome-wide association study of flowering time using a panel of 1490 cultivated soybean accessions.We identified three strong signals at the qFT02-2 locus(Chr02:12037319–12238569),which were associated with flowering time in three environments:Gongzhuling,Mengcheng,and Nanchang.By analyzing linkage disequilibrium,gene expression patterns,gene annotation,and the diversity of variants,we identified an AP1 homolog as the candidate gene for the qFT02-2 locus,which we named GmAP1d.Only one nonsynonymous polymorphism existed among 1490 soybean accessions at position Chr02:12087053.Accessions carrying the Chr02:12087053-T allele flowered significantly earlier than those carrying the Chr02:12087053-A allele.Thus,we developed a cleaved amplified polymorphic sequence(CAPS)marker for the SNP at Chr02:12087053,which is suitable for marker-assisted breeding of flowering time.Knockout of GmAP1d in the‘Williams 82’background by gene editing promoted flowering under long-day conditions,confirming that GmAP1d is the causal gene for qFT02-2.An analysis of the region surrounding GmAP1d revealed that GmAP1d was artificially selected during the genetic improvement of soybean.Through stepwise selection,the proportion of modern cultivars carrying the Chr02:12087053-T allele has increased,and this allele has become nearly fixed(95%)in northern China.These findings provide a theoretical basis for better understanding the molecular regulatory mechanism of flowering time in soybean and a target gene that can be used for breeding modern soybean cultivars adapted to different latitudes.展开更多
An analytic hierarchy process(AHP)was employed to establish a landscape value evaluation model for 20 common species of perennial flowers in the flower border of Hefei City.With the overarching objective of determinin...An analytic hierarchy process(AHP)was employed to establish a landscape value evaluation model for 20 common species of perennial flowers in the flower border of Hefei City.With the overarching objective of determining the application value of the flower border,10 evaluation factors were identified and subsequently classified into 3 distinct grades.Grade I(L>2.5)was defined as perennial flower resources with the highest development and utilization value,including only one species of Salvia farinacea.Grade II(2.0≤L≤2.5)was defined as perennial flower varieties suitable for most areas,encompassing 9 species of plants,such asTaraxacummongolicum.Grade III(L<2.0)was defined as perennial flower varieties with low application value,encompassing 10 species of plants,such asRuellia brittoniana,but lacking the value of further popularization and application.Consequently,the proportion of their application in the flower border should be reduced.The evaluation results can serve as a theoretical foundation for the implementation of perennial flowers in urban flower borders.展开更多
Anthocyanins play crucial roles in pollen protection and pollinator attraction in flowering plants.However,the mechanisms underlying flower color determination and whether floral anthocyanin regulators participate in ...Anthocyanins play crucial roles in pollen protection and pollinator attraction in flowering plants.However,the mechanisms underlying flower color determination and whether floral anthocyanin regulators participate in other processes remain largely unresolved in soybeans(Glycine max).In this study,we investigated the genetic components and mechanisms governing anthocyanin biosynthesis in soybean flowers.Molecular and genetic studies have characterized two antagonistic regulators,the positive activator GmMYBA3 and the negative repressor GmMYBR1,that modulate the gene expression of anthocyanin biosynthesis in soybean flowers.Further findings revealed a regulatory interplay between GmMYBA3 and GmMYBR1 bridged by GmTT8a,highlighting the complexity of anthocyanin regulation in different soybean organs.Exploration of additional soybean cultivars demonstrated the universality of GmMYBA3 and GmMYBR1 in regulating floral anthocyanin biosynthesis-related genes,with GmF3’5’H identified as a crucial determinant of white flower color.This study provides a molecular mechanism underlying soybean flower color determination,paving the way for the molecular modification of soybean flowers to probably enhance their resistance to abiotic stresses and attractiveness to pollinators.展开更多
The timing of flowering is an important driver of species distribution and community assembly patterns.However,we still have much to learn about the factors that shape flowering diversity(i.e.,number of species flower...The timing of flowering is an important driver of species distribution and community assembly patterns.However,we still have much to learn about the factors that shape flowering diversity(i.e.,number of species flowering per period) in plant communities.One potential explanation of flowering diversity is the mid-domain effect,which states that geometric constraints on species ranges within a bounded domain(space or time) will yield a mid-domain peak in diversity regardless of ecological factors.Here,we determine whether the mid-domain effect explains peak flowering time(i.e.,when most species of communities are flowering) across China.We used phenological data of 16,267 herbaceous and woody species from the provincial Flora in China and species distribution data from the Chinese Vascular Plant Distribution Database to determine relationships between the observed number of species flowering and the number of species flowering as predicted by the mid-domain effect model,as well as between three climatic variables(mean minimum monthly temperature,mean monthly precipitation,and mean monthly sunshine duration).We found that the mid-domain effect explained a significant proportion of the temporal variation in flowering diversity across all species in China.Further,the mid-domain effect explained a greater proportion of variance in flowering diversity at higher latitudes than at lower latitudes.The patterns of flowering diversity for both herbaceous and woody species were related to both the mid-domain effect and environmental variables.Our findings indicate that including geometric constraints in conjunction with abiotic and biotic predictors will improve predictions of flowering diversity patterns.展开更多
基金supported by National Key Research and Development Program for Intergovernmental International Science and Technology Innovation Cooperation(2024YFE0109500)National Natural Science Foundation of China(32072149).
文摘Gastric ulcer is a widespread disease caused by various etiologies.Dendrobium offi cinale flowers exert several health benefits owing to their rich flavonoid content.In this study,protective effects and possible action mechanisms of D.offi cinale flowers’flavonoid enriched extract(DOFF)were assessed against gastric ulcer.The result of sodium nitrite-aluminum nitrate colorimetry showed that 52.34%of the total extractive was flavonoid,and ultra-high performance liquid chromatography time of flight mass spectrometer(UPLC-QTOF/MS)revealed the presence of 28 components in DOFF of which 14 belonged to flavonoids.In addition,in vivo assay revealed DOFF potential in reducing the formation of ethanol-induced gastric mucosal lesions,with drop-in ulcer index from 64.33±8.76 to 32.00±4.47.Similar results were revealed in human gastric mucosal epithelia cells,with cells viability to increase from 27.2%to 61.6%post DOFF administration.To analyzed the protect effect of DOFF,we used Western blotting and immunofluorometric assay to revealed the expression levels of key proteins in cell pathways.The results showed that DOFF(320μg/mL)could increase the level of oxidation marker protein(HO-1),apoptosis regulatory protein(Bcl-2)and autophagy marker(LC3β)by 50.84%,43.85%,and 59.21%compared with ethanol-treated group respectively.Further analyzed of the mitochondrial activity and apoptosis pathway,we found that DOFF appeared to mitigate against ethanolinduced gastric mucosal injury via AMPK/mTOR/ULK1 and PI3K/AKT autophagy signaling pathways.
文摘Poetry,as a crucial form of literary expression,often employs metaphor as a common rhetorical device.According to the perspective of cognitive metaphor theory,metaphor transcends its traditional linguistic boundaries and is recognized as a profound cognitive mechanism,manifesting as a broader cognitive phenomenon.This article is based on metaphorical examples of“flowers”in Chinese and English poetry,carefully selecting representative cases for in-depth analysis.The aim is to compare the imagery of“flowers”in Chinese and English poetry,observe their similarities and differences,thereby fostering a better understanding of poetry in both languages.Through this study,we not only delve into the intricacies of metaphor within poetry but also shed light on the distinct interpretations of the symbol of“flowers”in different cultural contexts,expanding our appreciation for the cultural diversity inherent in poetry.
基金Sponsored by the Innovation Training Project for College Students in Anhui Province(S202212216130)Key Research Project of Natural Science in Universities of Anhui Province(2023AH051816)General Teaching Research Project of Anhui Province(2022jyxm665).
文摘In urban road greening,the planting of ground cover flowers is essential,and herbaceous flowers are an important part.Through the investigation on the diversity of herbaceous flowers in urban road green spaces of Hefei City,the types,colors,application frequency and planting of herbaceous flowers in urban road green spaces were statistically analyzed,and the application forms of herbaceous flowers in the road greening of Hefei City were studied.The characteristics and problems of herbaceous flowers in the road greening of Hefei City were found,and some development suggestions were put forward.
文摘Spatial narrative,as a unique literary narrative technique,focuses on advancing the plot and shaping characters through meticulous portrayal of space,providing readers with rich visual and perceptual experiences.This paper examines the spatial narrative utilized in Jin Yucheng’s novel,Flowers of Shanghai.Through an in-depth analysis of the spatial narrative techniques in the novel,this paper asserts that such an exploration has far-reaching consequences in broadening research perspectives and fostering interdisciplinary collaborations between literature and geography.Furthermore,the paper explores the unique aspects of narrative perspective,narrative structure,and narrative time in Flowers of Shanghai,and how these elements perfectly combine with spatial narrative to create a fascinating literary world for readers.The aim of this study is to deeply explore the artistic value and literary charm of the novel through a comprehensive interpretation of the spatial narrative in Flowers of Shanghai.
文摘The active ingredients of Camellia japonica flowers(CJF)at high and low altitudes,as well as their skin care efficacy were compared.The dried red CJF at high and low altitudes were ultrasonically extracted with 70%ethanol,and these extracts were concentrated and then diluted to a constant volume.The content of total flavonoids,total polyphenols and total proteins was tested and analyzed.In addition,DPPH free radical scavenging,inhibition of the formation of advanced glycation end products,and inhibitory activity against elastase was measured to compare their skin care efficacy in anti-oxidation,anti-glycation,anti-wrinkling and firming.The results showed that,based on the dry weight of CJF,the content of total flavonoids and total proteins of the CJF at high altitude was approximately 1.5 times of the CJF at low altitude,while the content of total polyphenols was approximately 2.4 times that of the CJF at low altitude.The skin care efficacy of CJF at high altitude was significantly better than that of the CJF at low altitude.This work could provide theoretical basis for the selection and application of Camellia japonica flowers in the field of cosmetics.
基金funded by National Key R&D Program of China(2022)Research on Precision Nutrition and Health Food,Department of Science and Technology of Henan Province(CXJD2021006)Key Project in Science and Technology Agency of Henan Province(212102310355).
文摘Flower plants are popular all over the world and important sources of ornamental plants,bioactive molecules and nutrients.Flowers have a wide range of biological activities and beneficial pharmacological effects.Flowers and their active ingredients are becoming more and more popular in the preparation of food,drugs and industrial products.This paper summarizes the active ingredients,pharmacological activities and applications in the pharmaceutical and food industries of flower plants in recent years.In addition,the possible molecular mechanism of pharmacological effects of flower plants were also discussed.302 active constituents from 55 species of flower plants were summarized,including flavonoids(115),terpenoids(90),phenylpropanoids(20),alkaloids(13),organic acids(27)and others(37).The pharmacological effects of flower plants are very extensive,mainly including antioxidant,anti-inflammatory,anti-tumor,anti-virus,and hypoglycemic.The mechanisms of anti-inflammatory,anti-tumor and hypoglycemic activities present the characteristics of multi-way and multi-target.Because of its rich nutrients,bioactive ingredients and plant essential oils,and its wide sources,flower plants are widely used in food,beverage,cosmetics and drug research.Flower plants also play an important role in pharmaceutical industry,food industry and other fields.
基金supported by the National Key Research and Development Plan“Research on protection and restoration of typical small populations of wild plants”(Grant No.2016YFC0503106)。
文摘Low temperature is among the most restrictive factors to limit the yield and distribution of pear. Pyrus hopeiensis is a valuable wild resource.PCA showed that P. hopeiensis had strong cold resistance. In this study, the mRNA and metabolome sequencing of P. hopeiensis flower organs exposed to different low temperatures were performed to identify changes of genes and metabolites in response to low-temperature stress. A total of 4 851 differentially expressed genes(DEGs) were identified. Trend analysis showed that these DEGs were significantly enriched in profiles 19, 18, 7, 14, 1, 4 and 11. And the KEGG enrichment analysis showed that the DEGs in profile 18 were significantly enriched in flavone and flavonol biosynthesis. Besides, the expressed trends as well as GO and KEGG functional enrichment analyses of DEGs under cold and freezing stress showed significantly difference. Analyses of flavonoid-related pathways indicated that flavonoid structural genes had undergone significant changes. Correlation analysis showed that b HLH and MYB TFs may affect flavonoid biosynthesis by regulating structural gene expression. And PhMYB308 and PhMYB330 were likely candidate repressors of flavonoid biosynthesis by binding to a specific site in bHLH proteins. In total, 92 differentially accumulated metabolites(DAMs) were identified in P. hopeiensis flowers including 12 flavonoids. WGCNA results showed that coral 1, pink and brown 4 modules were closely associated with flavonoids and 11 MYBs and 15 bHLHs among the three modules may activate or inhibit the expression of 23 structural genes of flavonoid biosynthesis. Taken together, the results of this study provided a theoretical basis for further exploration of the molecular mechanisms of flavonoid biosynthesis and cold resistance of P. hopeiensis flower organs and our findings laid a foundation for further molecular breeding in cold-resistant pear varieties.
基金partially supported by the National Natural Science Foundation of China (Grant No.31772285)the National Key R&D Program Project Funding (Grant No.2018YFD1000607)Foundation for 100 Innovative Talents of Hebei Province(Grant No.SLRC2019031)。
文摘The juvenile-to-adult phase change with first flowering as the indicator plays a crucial role in the lifecycle of fruit trees. However, the molecular mechanisms underlying phase change in fruit trees remain largely unknown. Shikimic acid (ShA) pathway is a main metabolic pathway closely related to the synthesis of hormones and many important secondary metabolites participating in plant phase change. So,whether ShA regulates phase change in plants is worth clarifying. Here, the distinct morphological characteristics and the underlying mechanisms of phase change in jujube (Ziziphus jujuba Mill.), an important fruit tree native to China with nutritious fruit and outstanding tolerance abiotic stresses, were clarified. A combined transcriptome and metabolome analysis found that ShA is positively involved in jujube(Yuhong’×Xing 16’) phase change. The genes in the upstream of ShA synthesis pathway (ZjDAHPS, ZjDHQS and ZjSDH), the contents of ShA and the downstream secondary metabolites like phenols were significantly upregulated in the phase change period. Further, the treatment of spraying exogenous ShA verified that ShA at a very low concentration (60 mg·L^(-1)) can substantially speed up the phase change and flowering of jujube and other tested plants including Arabidopsis, tomato and wheat. The exogenous ShA (60 mg·L^(-1)) treatment in jujube seedlings could increase the accumulation of endogenous ShA, enhance leaf photosynthesis and the synthesis of phenols especially flavonoids and phenolic acids, and promote the expression of genes (ZjCOs, ZjNFYs and ZjPHYs) involved in flowering pathway. Basing on above results, we put forward a propose for the underlying mechanism of ShA regulating phase change, and a hypothesis that ShA could be considered a phytohormone-like substance because it is endogenous, ubiquitous, movable and highly efficient at very low concentrations. This study highlights the critical role of ShA in plant phase change and its phytohormone-like properties.
基金This work was supported by the National Natural Science Foundation of China(31872140,31470693,31100503)the Natural Science Foundation of Zhejiang Province(LY18C160006)+1 种基金the Zhejiang Provincial Key Laboratory of Characteristic Aquatic Vegetable Breeding and Cultivation Open Fund Project(KL-2022-01,KL-2022-02)the Project for Science and Technology in Jinhua City(Grant No.2019-2-002).The authors thank TopEdit(www.topeditsci.com)for its linguistic assistance during the preparation of this manuscript.
文摘Nymphaea is a key genus of the ANA grade(Amborellales,Nymphaeales,and Austrobaileyales)of basal flowering plants,which serve as a key model to study the early evolution of floral traits.In this study,we comprehensively investigated the emission,biosynthesis,and biological function of the floral scent in a night-blossoming waterlily Nymphaea prolifera.The headspace volatile collection combined with GC-MS analysis showed that the floral scent of N.prolifera is predominately comprised by methylated benzenoids including anisole,veratrole,guaiacol,and methoxyanisole.Moreover,the emission of these floral benzenoids in N.prolifera exhibited temporal and spatial pattern with circadian rhythm and tissue specificity.By creating and mining transcriptomes of N.prolifera flowers,12 oxygen methyltransferases(NpOMTs)were functionally identified.By in vitro enzymatic assay,NpOMT3,6,and 7 could produce anisole and NpOMT5,7,9,produce guaiacol,whereas NpOMT3,6,9,11 catalyzed the formation of veratrole.Methoxyanisole was identified as the universal product of all NpOMTs.Expression patterns of NpOMTs provided implication for their roles in the production of the respective benzenoids.Phylogenetic analysis of OMTs suggested a Nymphaea-specific expansion of the OMT family,indicating the evolution of lineage-specific functions.In bioassays,anisole,veratrole,and guaiacol in the floral benzenoids were revealed to play the critical role in repelling waterlily aphids.Overall,this study indicates that the basal flowering plant N.prolifera has evolved a diversity and complexity of OMT genes for the biosynthesis of methylated benzenoids that can repel insects from feeding the flowers.These findings provide new insights into the evolutional mechanism and ecological significance of the floral scent from early-diverged flowering plants.
基金supported by the grants from the National Natural Science Foundation of China (32072590 and 32002051)the China Postdoctoral Science Foundation (2019M661244)the Academic Backbone Foundation of Northeast Agricultural University, China (20XG03)。
文摘Early flowering promotes early maturity, production, and the capacity to counteract biotic and abiotic stresses, making it an important agronomic trait in zucchini. The present study demonstrated that the zucchini inbred line ‘19’ consistently flowered early, taking significantly fewer days to bloom the first female flower(DFF) than the inbred line ‘113’. Genetic analysis revealed that DFF, an inheritable quantitative trait, is controlled by multiple genes. Based on the strategy of quantitative trait locus(QTL) sequencing(QTL-seq) combined with linkage analysis, three QTLs for DFF were identified on chromosomes 4, 11, and 20. This study used additional F2 populations grown under different environmental conditions for QTL mapping analysis of DFF with insertion/deletion(InDel) markers to validate these results. Using the composite interval mapping(CIM) method of R/qtl software, we only identified one major locus under all environmental conditions, located in a 117-kb candidate region on chromosome 20. Based on gene annotation, gene sequence alignment, and qRT-PCR analysis, we found that the Cp4.1LG20g08050 gene encoding a RING finger protein may be a candidate gene for the opposite regulation of early flowering in zucchini. In summary, these results lay a foundation for a better understanding of early flowering and improving early flowering-based breeding strategies in zucchini.
文摘This review summarizes the main chemical composition of Aloe vera flowers and introduces their antioxidant,anti-aging,skin whitening,skin moisturizing,anti-skin irritation,and anti-skin allergy effects.Suggestions for the application of Aloe v.flowers in cosmetics are provided.
文摘The article presents the biology of flowering and the daily dynamics of flowering of two species from the Lamiaceae family: ph. anisochila va ph. sogdiana of the distribution out in the Nuratau Mountains range.
基金supported by the National Key Research and Development Program of China(2023YFD1200600 to Xiaoya Lin)National Natural Science Foundation of China(32090060 to Fanjiang Kong,32001568 to Xiaoya Lin,31930083 to Baohui Liu,and 31901500 to Tiantian Bu)China Postdoctoral Science Foundation(2019 M652839 to Liyu Chen)。
文摘Soybean(Glycine max)is a short-day crop whose flowering time is regulated by photoperiod.The longjuvenile trait extends its vegetative phase and increases yield under short-day conditions.Natural variation in J,the major locus controlling this trait,modulates flowering time.We report that the three J-family genes influence soybean flowering time,with the triple mutant Guangzhou Mammoth-2 flowering late under short days by inhibiting transcription of E1-family genes.J-family genes offer promising allelic combinations for breeding.
基金supported by National Natural Science Foundation of China(Grant Nos.32372733,32172594)Natural Science Foundation of Hebei(Grant No.C2020204111)+2 种基金S&T Program of Hebei(Grant No.21326344D)State Key Laboratory of North China Crop Improvement and Regulation(Grant No.NCCIR2023ZZ-1)the Starting Grant from Hebei Agricultural University(Grant No.YJ201920).
文摘Properly regulated flowering time is pivotal for successful plant reproduction.The floral transition from vegetative growth to reproductive growth is regulated by a complex gene regulatory network that integrates environmental signals and internal conditions to ensure that flowering takes place under favorable conditions.Brassica rapa is a diploid Cruciferae species that includes several varieties that are cultivated as vegetable or oil crops.Flowering time is one of the most important agricultural traits of B.rapa crops because of its influence on yield and quality.The transition to flowering in B.rapa is regulated by several environmental and developmental cues,which are perceived by several signaling pathways,including the vernalization pathway,the autonomous pathway,the circadian clock,the thermosensory pathway,and gibberellin(GA)signaling.These signals are integrated to control the expression of floral integrators BrFTs and BrSOC1s to regulate flowering.In this review,we summarized current research advances on the molecular mechanisms that govern flowering time regulation in B.rapa and compare this to what is known in Arabidopsis.
基金supported by the National Natural Science Foundation of China(Grant No.32072609)National Key Research and Development Program of China(Grant No.2018YFD1000400)+3 种基金National Natural Science Foundation of China(Grant No.32002075)the China Postdoctoral Science Foundation(Grant No.2019M661871)the Fundamental Research Funds for the Central Universities(Grant No.KJQN202126)a project funded by the Priority Academic Program Development of Jiangsu Higher Education Institutions。
文摘Basic helix-loop-helix(bHLH)transcription factor gene family in plants controls various growth and development aspects;however,the actual roles of these genes in flowering plants are not well known.In this study,a novel bHLH protein CmbHLH110 was found to interact with CmERF110 by in vitro and in vivo experiments,a chrysanthemum ERF110 homolog that acts as a positive flowering regulator.In addition,CmbHLH110 was also found to regulate the flowering of chrysanthemums,overexpression of CmbHLH110 causes chrysanthemums to flower earlier,and suppressed CmbHLH110 leads to delayed flowering.Furthermore,the loss-of-function Arabidopsis mutant of its homologue PERICYCLE FACTOR TYPE-A 5(PFA5)had a noticeable late flowering phenotype,and CmbHLH110 completely complemented the late flowering phenotype of the pfa5 mutant,whereas heterologous overexpression of CmbHLH110 in Arabidopsis Col-0 caused early flowering.Transcriptome sequencing revealed significant differential expression of flowering-related and circadian clock-related genes in transgenic chrysanthemum.Therefore,we concluded that CmbHLH110,as a novel flowering regulator,could interact with CmERF110 to regulate flowering in chrysanthemum.
基金supported by the National Natural Science Foundation of China(U22A20473)the National Key Research and Development Program of China(2021YFD1201600)+2 种基金the China Agriculture Research System(CARS-04-PS01)the Agricultural Science and Technology Innovation Program(ASTIP)of Chinese Academy of Agricultural Sciences,Scientific Innovation 2030 Project(2022ZD0401703)the Platform of National Crop Germplasm Resources of China。
文摘Flowering time is important for adaptation of soybean(Glycine max)to different environments.Here,we conducted a genome-wide association study of flowering time using a panel of 1490 cultivated soybean accessions.We identified three strong signals at the qFT02-2 locus(Chr02:12037319–12238569),which were associated with flowering time in three environments:Gongzhuling,Mengcheng,and Nanchang.By analyzing linkage disequilibrium,gene expression patterns,gene annotation,and the diversity of variants,we identified an AP1 homolog as the candidate gene for the qFT02-2 locus,which we named GmAP1d.Only one nonsynonymous polymorphism existed among 1490 soybean accessions at position Chr02:12087053.Accessions carrying the Chr02:12087053-T allele flowered significantly earlier than those carrying the Chr02:12087053-A allele.Thus,we developed a cleaved amplified polymorphic sequence(CAPS)marker for the SNP at Chr02:12087053,which is suitable for marker-assisted breeding of flowering time.Knockout of GmAP1d in the‘Williams 82’background by gene editing promoted flowering under long-day conditions,confirming that GmAP1d is the causal gene for qFT02-2.An analysis of the region surrounding GmAP1d revealed that GmAP1d was artificially selected during the genetic improvement of soybean.Through stepwise selection,the proportion of modern cultivars carrying the Chr02:12087053-T allele has increased,and this allele has become nearly fixed(95%)in northern China.These findings provide a theoretical basis for better understanding the molecular regulatory mechanism of flowering time in soybean and a target gene that can be used for breeding modern soybean cultivars adapted to different latitudes.
基金Sponsored by Landscape Value Evaluation of Perennial Flower Border Application for Open Field Cultivation in Hefei City(S202212216134)Natural Science Key Research Program for Colleges and Universities in Anhui Province(2023AH051816)Anhui General Teaching Research Project(2022jyxm665).
文摘An analytic hierarchy process(AHP)was employed to establish a landscape value evaluation model for 20 common species of perennial flowers in the flower border of Hefei City.With the overarching objective of determining the application value of the flower border,10 evaluation factors were identified and subsequently classified into 3 distinct grades.Grade I(L>2.5)was defined as perennial flower resources with the highest development and utilization value,including only one species of Salvia farinacea.Grade II(2.0≤L≤2.5)was defined as perennial flower varieties suitable for most areas,encompassing 9 species of plants,such asTaraxacummongolicum.Grade III(L<2.0)was defined as perennial flower varieties with low application value,encompassing 10 species of plants,such asRuellia brittoniana,but lacking the value of further popularization and application.Consequently,the proportion of their application in the flower border should be reduced.The evaluation results can serve as a theoretical foundation for the implementation of perennial flowers in urban flower borders.
基金supported by the National Natural Science Foundation of China(32201781,32100211)the Department of Science and Technology of Jilin Province(20220508112RC,20210101005JC)+1 种基金the Fundamental Research Fund for the Central Universities(2412023YQ005)China Agriculture Research System(CARS04)。
文摘Anthocyanins play crucial roles in pollen protection and pollinator attraction in flowering plants.However,the mechanisms underlying flower color determination and whether floral anthocyanin regulators participate in other processes remain largely unresolved in soybeans(Glycine max).In this study,we investigated the genetic components and mechanisms governing anthocyanin biosynthesis in soybean flowers.Molecular and genetic studies have characterized two antagonistic regulators,the positive activator GmMYBA3 and the negative repressor GmMYBR1,that modulate the gene expression of anthocyanin biosynthesis in soybean flowers.Further findings revealed a regulatory interplay between GmMYBA3 and GmMYBR1 bridged by GmTT8a,highlighting the complexity of anthocyanin regulation in different soybean organs.Exploration of additional soybean cultivars demonstrated the universality of GmMYBA3 and GmMYBR1 in regulating floral anthocyanin biosynthesis-related genes,with GmF3’5’H identified as a crucial determinant of white flower color.This study provides a molecular mechanism underlying soybean flower color determination,paving the way for the molecular modification of soybean flowers to probably enhance their resistance to abiotic stresses and attractiveness to pollinators.
基金supported by the National Natural Science Foundation of China (Grant number 42261004)supported by the Jiangsu Social Development Project (BE2022792)。
文摘The timing of flowering is an important driver of species distribution and community assembly patterns.However,we still have much to learn about the factors that shape flowering diversity(i.e.,number of species flowering per period) in plant communities.One potential explanation of flowering diversity is the mid-domain effect,which states that geometric constraints on species ranges within a bounded domain(space or time) will yield a mid-domain peak in diversity regardless of ecological factors.Here,we determine whether the mid-domain effect explains peak flowering time(i.e.,when most species of communities are flowering) across China.We used phenological data of 16,267 herbaceous and woody species from the provincial Flora in China and species distribution data from the Chinese Vascular Plant Distribution Database to determine relationships between the observed number of species flowering and the number of species flowering as predicted by the mid-domain effect model,as well as between three climatic variables(mean minimum monthly temperature,mean monthly precipitation,and mean monthly sunshine duration).We found that the mid-domain effect explained a significant proportion of the temporal variation in flowering diversity across all species in China.Further,the mid-domain effect explained a greater proportion of variance in flowering diversity at higher latitudes than at lower latitudes.The patterns of flowering diversity for both herbaceous and woody species were related to both the mid-domain effect and environmental variables.Our findings indicate that including geometric constraints in conjunction with abiotic and biotic predictors will improve predictions of flowering diversity patterns.