BACKGROUND:The genetic diversity of chemokines and chemokine receptors has been associated with the outcome of hepatitis B virus infection.The aim of this study was to evaluate whether the copy number variation in the...BACKGROUND:The genetic diversity of chemokines and chemokine receptors has been associated with the outcome of hepatitis B virus infection.The aim of this study was to evaluate whether the copy number variation in the CCL3L1 gene and the polymorphisms of CCR5Δ32 and CCR5-2459A→G (rs1799987) are associated with recurrent hepatitis B in liver transplantation for hepatitis B virus infection-related end stage liver disease.METHODS:A total of 185 transplant recipients were enrolled in this study.The genomic DNA was extracted from whole blood,the copy number of the CCL3L1 gene was determined by a quantitative real-time PCR based assay,CCR5Δ32 was detected by a sizing PCR method,and a single-nucleotide polymorphism in CCR5-2459 was detected by restriction fragment length polymorphism PCR.RESULTS:No CCR5Δ32 mutation was detected in any of the individuals from China.Neither copy number variation nor polymorphism in CCR5-2459 was associated with post-transplant reinfection with hepatitis B virus.However,patients with fewer copies (<4) of the CCL3L1 gene compared with the population median in combination with the CCR5G allele had a significantly higher risk for recurrent hepatitis B (odds ratio=1.93,95% CI:1.00-3.69;P=0.047).CONCLUSION:Patients possessing the compound decreased functional genotype of both CCL3L1 and CCR5 genes might be more likely to have recurrence of hepatitis B after transplantation.展开更多
基金supported by grants from the National Basic Research Program of China (973 Program) (2009CB522403)the National S&T Major Project (2008ZX10002-026)
文摘BACKGROUND:The genetic diversity of chemokines and chemokine receptors has been associated with the outcome of hepatitis B virus infection.The aim of this study was to evaluate whether the copy number variation in the CCL3L1 gene and the polymorphisms of CCR5Δ32 and CCR5-2459A→G (rs1799987) are associated with recurrent hepatitis B in liver transplantation for hepatitis B virus infection-related end stage liver disease.METHODS:A total of 185 transplant recipients were enrolled in this study.The genomic DNA was extracted from whole blood,the copy number of the CCL3L1 gene was determined by a quantitative real-time PCR based assay,CCR5Δ32 was detected by a sizing PCR method,and a single-nucleotide polymorphism in CCR5-2459 was detected by restriction fragment length polymorphism PCR.RESULTS:No CCR5Δ32 mutation was detected in any of the individuals from China.Neither copy number variation nor polymorphism in CCR5-2459 was associated with post-transplant reinfection with hepatitis B virus.However,patients with fewer copies (<4) of the CCL3L1 gene compared with the population median in combination with the CCR5G allele had a significantly higher risk for recurrent hepatitis B (odds ratio=1.93,95% CI:1.00-3.69;P=0.047).CONCLUSION:Patients possessing the compound decreased functional genotype of both CCL3L1 and CCR5 genes might be more likely to have recurrence of hepatitis B after transplantation.
