[Objectives]The purpose was to determine the contents of quercetin and kaempferol in Flueggea virosa(Roxb.ex Wild.)Baill.,thereby providing reference data for the research and application of F.virosa.[Methods]Querceti...[Objectives]The purpose was to determine the contents of quercetin and kaempferol in Flueggea virosa(Roxb.ex Wild.)Baill.,thereby providing reference data for the research and application of F.virosa.[Methods]Quercetin and kaempferol in F.virosa were extracted with ultrasonic method,using dilute hydrochloric acid-methanol(1∶10)solution as the extraction solvent,and their contents were determined with RP-HPLC.[Results]The contents of quercetin and kaempferol in F.virosa were 0.2336 and 0.3272 mg/g,respectively.[Conclusions]The method has high speed,high resolution,high sensitivity,accurate and reliable detection results,and can be used as a method for the determination of quercetin and kaempferol in F.virosa.展开更多
基金Natural Science Research Project of Guangxi University of Chinese Medicine(P2012025)Guangxi University Laboratory of Extraction,Purification and Quality Analysis of Traditional Chinese Medicine(J1606701).
文摘[Objectives]The purpose was to determine the contents of quercetin and kaempferol in Flueggea virosa(Roxb.ex Wild.)Baill.,thereby providing reference data for the research and application of F.virosa.[Methods]Quercetin and kaempferol in F.virosa were extracted with ultrasonic method,using dilute hydrochloric acid-methanol(1∶10)solution as the extraction solvent,and their contents were determined with RP-HPLC.[Results]The contents of quercetin and kaempferol in F.virosa were 0.2336 and 0.3272 mg/g,respectively.[Conclusions]The method has high speed,high resolution,high sensitivity,accurate and reliable detection results,and can be used as a method for the determination of quercetin and kaempferol in F.virosa.