The interaction of trypsin with camptothecin(CPT) in vitro was studied by ultraviolet(UV) absorption spectral and fluorescence spectral methods.Making out value of Ki according to the ratio between 1/v and the amount ...The interaction of trypsin with camptothecin(CPT) in vitro was studied by ultraviolet(UV) absorption spectral and fluorescence spectral methods.Making out value of Ki according to the ratio between 1/v and the amount of inhibitor contributes to the conclusion that CPT is a noncompetitive inhibitor.The interaction between CPT and trypsin is quite strong.CPT can affect the conformation of trypsin in some degree.Fluorescence quenching contributes to nonradiative energy-transfer,which results a static quenching of CPT to trypsin.Their binding constants and the binding sites of CPT were determined.展开更多
采用三维荧光分析方法,结合芳烃馏分分析结果,对胡庆油田油藏中原油、油砂和烃源岩展开了对比研究。研究结果表明,油藏中原油和油砂的三维荧光光谱具有5种类型,烃源岩存在3种类型光谱特征,所有油藏的主波长低于 Type C类烃源岩,而高于 T...采用三维荧光分析方法,结合芳烃馏分分析结果,对胡庆油田油藏中原油、油砂和烃源岩展开了对比研究。研究结果表明,油藏中原油和油砂的三维荧光光谱具有5种类型,烃源岩存在3种类型光谱特征,所有油藏的主波长低于 Type C类烃源岩,而高于 Type A 和 Type B 类烃源岩。因此确定,广泛分布于胡庆油田二台阶不同断块带的原油是早期成烃旋回期间低成熟、成熟原油混合的结果。展开更多
基金Supported by the Project of Cultivating Innovation inGraduate Student of Jiangsu Province (2004) the Teaching and Research Award Program for OutstandingYoung Professor in Higher Education Institute (2003-2008)
文摘The interaction of trypsin with camptothecin(CPT) in vitro was studied by ultraviolet(UV) absorption spectral and fluorescence spectral methods.Making out value of Ki according to the ratio between 1/v and the amount of inhibitor contributes to the conclusion that CPT is a noncompetitive inhibitor.The interaction between CPT and trypsin is quite strong.CPT can affect the conformation of trypsin in some degree.Fluorescence quenching contributes to nonradiative energy-transfer,which results a static quenching of CPT to trypsin.Their binding constants and the binding sites of CPT were determined.
文摘采用三维荧光分析方法,结合芳烃馏分分析结果,对胡庆油田油藏中原油、油砂和烃源岩展开了对比研究。研究结果表明,油藏中原油和油砂的三维荧光光谱具有5种类型,烃源岩存在3种类型光谱特征,所有油藏的主波长低于 Type C类烃源岩,而高于 Type A 和 Type B 类烃源岩。因此确定,广泛分布于胡庆油田二台阶不同断块带的原油是早期成烃旋回期间低成熟、成熟原油混合的结果。
