Clinical application of indocyanine green fluorescence imaging in laparoscopic cholecystectomy with common bile duct exploration and J-Tube drainage

BACKGROUND Intraoperative and postoperative biliary injuries remain significant complications of laparoscopic common bile duct exploration(LCBDE).Indocyanine green(ICG)has been shown to significantly reduce injuries caused by intraoperative operational errors.We found that the J-tube can reduce postoperative strictures and injuries to the common bile duct.At this moment,we aim to analyze and compare the complications,efficacy,short-term outcomes,and feasibility of these two adjunctive tools for LCBDE.AIM To evaluate the efficacy of ICG fluorescence imaging In LCBDE and J-tube drainage for patients with common bile duct stones.METHODS We retrospectively collected the clinical case data of patients who were treated at the Hepatobiliary Surgery Department of the Third People’s Hospital of Nantong,affiliated with Nantong University,from January 2016 to January 2021 due to gallbladder stones with choledocholithiasis and who underwent LCBDE combined with a primary suture and either J-tube or T-tube drainage.The patients were divided into groups:Traditional white-light laparoscopy+T-tube group(WL+T-tube),traditional WL+J-tube group,fluorescent laparoscopy+T-tube group(ICG+T-tube)and fluorescent laparoscopy+J-tube group(ICG+J-tube).The preoperative and postoperative clinical case data,laboratory examination data,and intraoperative and postoperative complications(including postoperative bile leakage,electrolyte disturbances,biliary peritonitis,and postoperative infections)and other relevant indicators were compared.RESULTS A total of 198 patients(112 males and 86 females)were included in the study,with 74 patients in the WL+T-tube,47 in the WL+J-tube,42 in the ICG+T-tube,and 35 in the ICG+J-tube.Compared with the other groups,the ICG+J had significantly shorter operation time(114 minutes,P=0.001),less blood loss(42 mL,P=0.02),shorter postoperative hospital stays(7 days,P=0.038),and lower surgical costs(China yuan 30178,P=0.001).Furthermore,patients were subdivided into two groups based on whether a T-tube or J-tube was placed during the surgery.By the third postoperative day,the aspartate transaminase,glutamic pyruvic transaminase,total bilirubin,and direct bilirubin levels were lower in the J-tube group than in the T-tube group(P<0.001).At last,follow-up observations showed that the incidence of biliary strictures at three months postoperatively was significantly lower in the J-tube group than in the T-tube group(P=0.002).CONCLUSION ICG fluorescence imaging in laparoscopic cholecystectomy with common bile duct exploration and J-tube drainage facilitates rapid identification of biliary anatomy and variations,reducing intraoperative bile duct injury,blood loss,surgery duration,and postoperative bile duct stenosis rates,supporting its clinical adoption.

STUDY ON THE MECHANISM OF FLUORESCENCE QUENCHING OF NITROCOMPOUNDS FOR OXAZOLES *

The mechanism of nitrocompounds quenching of the fluorescence of 5, 5′ dipheny1 2, 2′ bisoxazole (POOP) and trans 1, 2 bis [2 (5 phenyloxazolyl)] ethene (POEOP) has been studied. It was found that the fluorescence of oxazoles was quenched mainly by the absorption competition and radiationless energy transfer of nitrocompounds. The fluorescence quenching rate constants of nitrobenzene and nitromethane are 3.0×10 10 L·mol -1 ·s -1 and 1.5×10 8 L·mol -1 ·s -1 respectively for POEOP. This remarkable difference was explicated.

Aggregation-dependence of Optical Properties and Fluorescence Quenching of a Water-soluble Poly(phenylene ethynylene)

The solvent and surfactant-dependent aggregation behaviors of the anionic poly（phenylene ethynylene） were studied and used to tune optical properties and fluorescence quenching efficiency.

Ultrafast Fluorescence Quenching in LnFe(CN)_6·4H_2O

对ＬｎＦｅ（ＣＮ）６·４Ｈ２Ｏ（Ｌｎ＝Ｅｕ，Ｔｂ）体系的吸收光谱和荧光光谱的研究表明，两配合物的中Ｅｕ３＋和Ｔｂ３＋离子的荧光发生强烈猝灭，即使是用高功率激光直接激发Ｅｕ３＋的７Ｆ０→５Ｄ０跃迁也是如此，表明该体系中稀土离子的荧光寿命非常短，有可能满足室温超快相干控制的要求．

Fluorescence quenching method for the determination of catechol with gold nanoparticles and tyrosinase hybrid system

The determination method of catechol by fluorescence quenching was developed.The assay was based on the combination of the unique property of gold nanoparticles with tyrosinase enzymatic reaction.In the presence of tyrosinase,the fluorescence of gold nanoparticles was quenched by catechol which can be employed to detect catechol.Under the optimal conditions,a linear range 5.0×10^(-7)-1.0×10^(-3) mol L^(-1) and a detection limit 1.0×10^(-7) mol L^(-1) of catechol were obtained.o-Quinone intermediate produced...

Fluorescence Quenching of a Novel Eu(Ⅲ) Coordination Compound for the Detection of Trace Amounts of Water： Synthesis, Structure and Photoluminescent Property

A novel luminescent coordination compound Eu（TTA）3（DEDAF）（1, TTA = 1,1,1-trifluoro-3-（2-thenoyl）acetone, DEDAF = 9,9-diethyl-4,5-diazafluoren） has been synthesized and fully characterized by infrared spectrum, elemental analysis, UV-vis spectrum, etc. X-ray single-crystal diffraction analysis reveals that compound 1 shows a mononuclear structure with the europium atom in coordinating to one DAF and three TTA ligands. The mononuclear structure units are assembled into a 3-D polymer by hydrogen bonds and π-π interactions. Photoluminescent property of 1 was investigated in detail at room temperature. Complex 1 emits strong red luminescence. However, it could be quenched even by small amount of water. The fluorescence intensity at 614 nm decreases linearly with the water content increasing（vol% in acetonitrile） in the range of 0.025~0.2% under 278 nm excitation. Thermogravimetric analysis has also been studied, which demonstrates good thermal stability of 1.

Fluorescence Quenching of Anthracene by N,N-Diethylaniline and Phenothiazine in Triton X-100/n-C_(10)H_(21) OH/H_2O Microemulsion

The photo-induced electron transfer reactions of anthracene with N,N -diethylaniline(DEA) and phenothiazine(PTZ) occur in the membrane phase of a Triton X-100/ n -C 10 H 21 OH(1-decanol)/H 2O microemulsion. DEA and PTZ exist in the membrane phase of the microemulsion. Anthracene exists in the oil continuous phase of the W/O microemulsion and in the oil core and membrane phase of the O/W microemulsion.

FLUORESCENCE AND ITS ENHANCEMENT OR QUENCHING OF Eu(TTA)_3 Phen LB FILMS

For the first time, LB films containing Eu(TTA)3Phen are obtained by using composite subphase. Eu(TTA)3Phen itself is dimcult to be transferred onto solid substrate.mixed or alternate LB films are prepared. Hydrogen bonds form between Eu(TTA)3Phen and AA or TPP(-COOH). TOPO and SB have a fluorcscence enhanGement effect on Eu(TTA)3Phen. TPP(-COOH) and Fc have a fluorescence quenching effect on it.

Determination of epristeride by its quenching effect on the fluorescence of L-tryptophan

A rapid, novel spectrofluorimetric method to determine epristeride （EP） in biological fluids and a pharmaceutical formulation was developed, based on the fact that fluorescence intensity of L-tryptophan could be quenched by EP in the medium of pH=9.0. The various factors influencing fluorescence quenching were discussed. The quenching mechanism was investigated with the quenching type, synchronous fluorescence spectra and quantum efficiency. Under the optimized conditions, fluorescence quenching value （AF^---FL_tryptophan--FEP_L_tryptophan） showed a good linear relationship with the EP concentration ranging from 0.4 to 12.0 lag/mL. The linearity, recovery and limit of detection demonstrated that the proposed method was suitable for EP determination in biological fluids and EP tablets. The method was successfully applied to the analysis of EP in real samples and the obtained results were in good agreement with the results of the official method.

Spectroscopic Characterization of Eu-Enoxacin-Phen Complexes and Determination of ATP by Fluorescence Quenching

The complex of Eu 3+ and Enoxacin(ENX) and Phen was synthesized. Its compositions is Eu(ENX)_3(phen)Cl_3·2H_2O. Its fluorescence spectra were studied. The results show that the strong fluorescence emitting of 592 and 616 nm for Eu 3+ in the Eu(ENX)_3(phen)Cl_3·2H_2O complex is related to the transitions 5D_0-7F_1 and 5D_0-7F_2 respectively. A sensitive method for the determination of adenosine triphosphate(ATP) by fluorescence quenching is proposed. The method is based on the ability of ATP to inhibit the formation of a strong fluorescent complex of Eu(ENX)_3(phen)Cl_3·2H_2O. In conditions of pH 6.7 in HAc-NaAc buffer and λ_ ex=340 nm and λ_ em=616 nm, the linear range of the determination is 0.4～ 10 μg·ml -1 and the detection limit is 0.034 μg·ml -1 for ATP. The method was applied to determine ATP in adenosine disodium triphosphate injection samples with relative standard deviation of 212% and recovery of 94.8%～1037%.

Absorption,quenching,and enhancement by tracer in acetone/toluene laser-induced fluorescence

To measure the equivalent ratio distribution of the two-stage lean premixed(DLP)flame,we propose using acetone/toluene planar laser-induced fluorescence(PLIF)technology to simultaneously measure the concentrations of the two components.Appropriate excitation laser wavelength and filters are used to assess the influence of acetone and toluene on each other’s fluorescence signal at room temperature.Experimental results show that acetone has a strong absorption effect on toluene’s fluorescence signal,the effective absorption cross-section is 5.77×10-20 cm-2.Acetone has an obvious quenching effect on the toluene fluorescence signal,and the Stern–Volmer coefficient is 0.50 kPa-1.The collisions between the molecules of toluene and acetone will lead to the enhancement of the fluorescence signal of acetone,and the enhancement coefficient is exponential with the acetone’s concentration.The quantitative relationship between the fluorescence intensity and the concentrations of the two tracers is obtained by establishing the photophysical model of toluene and acetone’s fluorescence signals.

Synthesis and Fluorescence of Eu-Ibuprofenum-Phen Complex and Determination of ATP by Fluorescence Quenching of Complex

The complex Eu(IB)_3(phen)·2H_2O was synthesized. The results show that the strong fluorescence emitting of 593 and 615 nm for Eu^(3+) in the Eu(IB)_3(phen) complex is related to the transitions (()~5D_0-()~7F_1) and (()~5D_0-()~7F_2,) respectively. A sensitive method for the determination of adenosine disodium triphosphate (ATP) by fluorescence quenching is proposed. The method is based on the ability of ATP to inhibit the formation of a strong fluorescent complex of Eu(IB)_3(phen). In conditions of pH 6.7 HAc-NaAc buffer, λ_(ex)=273 nm and λ_(em)=615 nm, the linear range of the determination was 0.1～10 μg·ml^(-1) and the detection limit was 0.03 μg·ml^(-1) for ATP. The method has been applied to determine ATP in adenosine disodium triphosphate tablets samples with relative standard deviation of 2.18% and recovery of (98.4%～)104.0%.

Mechanisms of Fluorescence Quenching of Pr^(3+)-Doped PbWO_4 Crystal

Fluorescence decay curves of the ^3P0 and ^1D2 manifolds in Pr^3＋ doped PbWO4 crystal were measured at room temperature and fluorescence lifetimes of both manifolds were estimated. Combining with the radiative lifetimes of the manifolds calculated on the basis of the modified J-O theory, the main mechanisms for the fluorescence quenching of the manifolds were analyzed. The multi-phonon relaxation and the cross-relaxation energy transfer are the major reasons for the fluorescence quenching of the ^3P0 and ^1D2 manifolds, respectively. The Inokuti-Hirayama model was used to analyze the fluorescence decay curve of the ^1D2 manifold and the cross-relaxation of dlpole-dipole interaction was confirmed. Consequently, the ^3p0 manifold is more favorable as an upper laser level than the ^1D2 manifold.

Amplified Detection of Iron Ion Based on Plasmon Enhanced Fluorescence and Subsequently Fluorescence Quenching

A facile and rapid approach for detecting low concentration of iron ion(Fe3+) with improved sensitivity was developed on the basis of plasmon enhanced fluorescence and subsequently amplified fluorescence quenching.Au1Ag4@Si O2 nanoparticles were synthesized and dispersed into fluorescein isothiocyanate(FITC) solution. The fluorescence of the FITC solution was improved due to plasmon enhanced fluorescence. However, efficient fluorescence quenching of the FITC/Au1Ag4@Si O2 solution was subsequently achieved when Fe3+, with a concentration ranging from17 n M to 3.4 l M, was added into the FITC/Au1Ag4@Si O2 solution, whereas almost no fluorescence quenching was observed for pure FITC solution under the same condition. FITC/Au1Ag4@Si O2 solution shows a better sensitivity for detecting low concentration of Fe3+compared to pure FITC solution. The quantized limit of detection toward Fe3+was improved from 4.6 l M for pure FITC solution to 20 n M for FITC/Au1Ag4@Si O2 solution.

Laser-Induced Recoverable Fluorescence Quenching of Perovskite Films at a Microscopic Grain Scale

Understanding the fundamental properties of metal-halide perovskite materials is driving the development of novel optoelectronic applications.Here,we report the observation of a recoverable laser-induced fluorescence quenching phenomenon in perovskite films with a microscopic grain-scale restriction,accompanied by spectral variations.This fluorescence quenching depends on the laser intensity and the dwell time under Auger recombination dominated conditions.These features indicate that the perovskite lattice deformation may take the main responsibility for the transient and show a new aspect to understand halide perovskite photostability.We further modulate this phenomenon by adjusting the charge carrier recombination and extraction,revealing that efficient carrier transfer can improve the bleaching resistance of perovskite grains.Our results provide future opportunities to attain high-performance devices by tuning the perovskite lattice disorder and harvesting the energetic carriers.

Synthesis and Fluorescence Quenching of Copolymer Containing3, 4, 9, 10-Perylenetetracarboxyl Diimide Side Chains

A photoluminescence material, copolymer of N-vinylcarbazole (VCz) and N,N'- bisallyl-3, 4. 9, 10-ferylenetetracarboxyl diimide (APTC) chromophores P(VCz-APTC) was synthesized. The fluorescence quenching of P(VCz-APTC) by fullerene (C-60) and triethylamine was also studied.

Study on State of Terbium Ion in Liposome by One order Derivative Fluorescence Quenching Method

The state of Tb3+ is investigated in liposome. When the concentration of PC is below CMC (critical micell concentration), most of Tb3+ is associated with PC, the binding constant is about 3.35×103 L/mol. When the concentration of PC is beyond CMC, most of Tb3+ is dimerized, the dimerization constant is about 3.92×104L/mol. In PC?CH?H2O system, the binding constant of Tb3+?CH complex 2.93×104L/mol is obtained.

A FLUORESCENCE QUENCHING IMNUNOASSAY METHOD FOR DETERMINATION OF TRACE ALBUMIN USING UNLABELED EUROPIUM CHELATE

A fluoroimmunoassay method using unlabeled europium chelate is described.The principle is similar to that of fluoroimmunoassay method using lanthanide chelate as labels.The procedure is simple because labeling process is omitted.The detection limit is about 10^(10) mol/L antigen.The relative standard deviation of immunoassay is less than 10%.The recoveries of human serum albumin and estradiol protein conjugate are 96-105% and 111% respectively.

STUDY ON THE SOLUTION FLUORESCENCE QUENCHING FOR POLYSILANES

he solutionfluorescence quenching mechanisms of poly(methylcyclohexylsilane),poly(methylphenethylsilane), poly(methylcyclohexylsilanecomethylphenethylsilane) and poly(methylphenylsilane) are studied by means of steadystate fluorescence,timeresolved fluorescence and UV absorption. The results show that the fluorescence quenching for polysilanes by carbon tetrachloride is the static quenching with energy migration, which is also supported by the qualitative results of the fluorescence quenching behaviors of polysilane by nitromethane (CH3NO2) and 2, 4, 7trinitrofluorenone(TNF).

A NEW KIND OF SURFACTANT:LONG-CHAIN ALKYLTRIPHENYLPHOSPHONIUM HALIDES Ⅲ.AN“ABNORMAL”QUENCHING OF PYRENE FLUORESCENCE BY PHOSPHONIUMS WITH DIFFERENT LENGTHS OF ALKYL CHAIN

A study of the quenching of pyrene fluorescence by a series of alkyltriphenylphosphonium bromides indi- cated the coiling conformation of ionic surfactant in aqueous solution.Formation of pyrene excimer in cetyltriph- enylphosphonium solution strengthened the conclusion.