Construction of Cox7a2 fluorescent vector and its effect on cytochrome C oxidase activity in mouse Sertoli cell line TM4

Objective To construct Cox7a2 fluorescent vector and study its effect on cytochrome C oxidase ( COX) activity in mouse Sertoli cell line TM4. Methods The coding region of CoxTa2 was amplified from mouse Sertoli cell line TM4 by RT-PCR. PCR product was

STUDIES ON FLUORESCENCE ENHANCING EFFECT AND ALKALINE DEGRADATION MECHANISM OF DOXYCYCLINE AND METHACYCLINE

Degradation reaction of doxycycline or methacycline was carried out in KOH solution and intense fluorescence was obtained.A degradation mechanism of doxycycline or methacycline was suggested.

All-fiber Based Er^(3+)∶Yb^(3+) Co-doped Fiber Laser

An all-fiber based Er~ 3+ ∶Yb~ 3+ co-doped double clad fiber laser operating at 1550nm is demonstrated. By using 9m long Er~ 3+ ∶Yb~ 3+ co-doped fiber(EYDF) as the gain medium, and using a pair of fiber Bragg gratings as wavelength filters, the line-width of the output laser is as narrow as 0.2nm and the output power is more than 6mW. The fluorescent effect of the laser before its emission is also studied. And it is found that the Er~ 3+ ∶Yb~ 3+ co-doped double-clad fiber laser also exhibits a high gain for Yb~ 3+ transition near 1080nm.

Confinement fluorescence effect of an aggregation-induced emission luminogen in crystalline polymer

Despite the impressive progress of stimuli-responsive fluorescent materials,little is known about the influence of confinement created by crystalline polymer over the fluorescence properties of fluorescent molecules.The effects of confinement on the fluorescence of an aggregation-induced emission luminogen(AIEgen)are investigated using computational simulations,which reveal that the confined space induces the AIEgens to take a more planar conformation,resulting in a red-shifted emission spectrum.With this property,the study is extended to explore the confinement generated by various polymer crystalline forms,and it is shown that different fluorescence colors are activated.This confinement fluorescence effect is attributed to the different spatial dimensions of the polymer amorphous layer between lamellar crystals where the AIEgens are located.These results indicate the immediate association between crystalline structure and fluorescence signals,activating unprecedented photophysical properties of luminescent materials,and also providing the possibility for crystalline structure visualization,it is important for the many polymer crystallization processes occurring in the materials processing.

Water-soluble Porphyrin as Temperature Sensor Based on Fluorescent Enhancement

A novel water-soluble porphyrin[5,10,15,20-tetra（3-ethoxy-4-hydroxy-5-sulfonate）phenyl porphyrin, H2TEHPPS] was designed and synthesized, which could be used as a potential fluorescence sensor to detect temperature changes. The studies were performed in solution phase and the concentration of H2TEHPPS was 2.0×10^-5 mol/L. The optical properties of H2TEHPPS were investigated based on the UV and fluorescence spectra. The results show that the fluorescence intensity of H2TEHPPS is directly proportional to temperature in the range of 293-353 K So H,TEHPPS can be used as a molecular temoerature sensor in biomedical and other fields.

Communication Chain-foldability effect on coaggregation Evidence derived from time-resolved fluorescence spectroscopy

Very recently,we have proposed that all culprit molecules of arteriosclerosis mightpossess a common and measurable inherent property,namely,a coaggregating ten-dency.Extensive kinetic measurements of these tendencies in terms of △CAgC valueshave led to a new and surprising observation,i.e.,the cholesteryl ester with a longsaturated 18-carbon chain(CE-18)actually has a much smaller tendency towardcoaggregation than that of the ester with a“short”8-carbon chain(CE-8).Thisstate of affairs has been coined as the“chain-foldability effect”,i.e.,the 18-carbon

A new fluorescence probing strategy for the detection of parathion-methyl based on N-doped carbon dots and methyl parathion hydrolase

A new facile fluorescence probing strategy, which was based on N-doped carbon dots（NCDs） and methyl parathion hydrolase（MPH）, was developed for the determination of parathion-methyl（PM）. The fluorescence intensity of NCDs-MPH system was proportional to PM concentration in the range of 2.38–73.78 mmol/L, with a detection limit of 0.338 mmol/L. Moreover, the present simple and facile method could be used to determine methyl parathion in environmental and agricultural samples successfully.Furthermore, the detection mechanism of this system is inner filter effect and molecular interactions between NCDs and p-nitrophenol, which is the hydrolysis product of PM catalyzed by methyl parathion hydrolase.

Syntheses and structures of tetranuclear Zn(Ⅱ) complexes with in situ generated macrocyclic Schiff base ligands:Applications in Zn^(2+) sensing

Three novel Zn（II） complexes,[Zn4L1Cl4]-3H2O（1）,[Zn4L2Cl4]-2DMF（2） and[Zn4L^3Cl4]H2O（3）,have been synthesized and structurally characterized.In these complexes,interesting 32-membered dodecadentate macrocyclic ligands were generated in situ by ＇2 ＋ 2＇ type condensation reactions between a tetraamine and various dialdehydes.All the complexes are isostructurally tetranuclear Zn（Ⅱ） complexes,containing endogenous alkoxo and phenoxo bridges.Applications of the macrocyclic ligands as Zn^2＋ sensors have been investigated.Take H4L^1 for example,it exhibits a 4-fold fluorescence enhancement upon the addition of 2 equiv.of Zn^2＋ in MeOH.