The Eu-Tetracycline(TC)-TOPO-sodium dodecyl sulfonate(SDS)system was studied,Experiments showed that the maximum fluorescence intensity was obtained in the pH range of 7.5-8.7 and the concentrations of Eu^(a+),TOPO an...The Eu-Tetracycline(TC)-TOPO-sodium dodecyl sulfonate(SDS)system was studied,Experiments showed that the maximum fluorescence intensity was obtained in the pH range of 7.5-8.7 and the concentrations of Eu^(a+),TOPO and SDS are 1.0×10^(-6)mol/L,1.0×10^(-8)mol/L and 1.0×10^(-8)mol/L,respectively,This fluorescence system can be used for the determination of TC in serum and urine,Beer's Law is obeyed in the range of 2.0×10^(-8)mol/L=1.0×10^(-6)mol/L for the concentration of TC. The determination limit is 1.2×10^(-8)mol/L,The composition and the luminescence mechanism were discussed.展开更多
A fluorimetric method for the determination of tyrosine in serum was proposed. The fluoriscence intensity is a linear function of tyrosine content in the range 0-1. 44ug/ml. The percentage of recovery was satisfactory.
A dehydration reaction of ADM was carried out in sulfuric acid solution and an intensely fluorescent product was formed.This reaction was applied to the determination of ADM in body fluids.
Methylmercury is the most toxic one among the mercury species. Many methods including atomic absorption spectrometry, atomic emission spectrometry, atomic fluorescence spectrometry, gas chromatography, high\|performa...Methylmercury is the most toxic one among the mercury species. Many methods including atomic absorption spectrometry, atomic emission spectrometry, atomic fluorescence spectrometry, gas chromatography, high\|performance liquid chromatography, ion chromatography, and capillary electrophoresis, have been published for the determination of methylmercury. These methods have been reviewed (Li, 1997; 1993; Niu, 1991). These methods require expensive equipment and highly qualified analysts. Although fluorimetric method is sensitive, inexpensive, easy to operate, no data about determination of methylmercury by fluorimetry can be found in the literature. In order to obtain a better method for the determination of methylmercury by fluorimetry, several reagents such as 8\| hydroxyquinoline, 8\|aminoquinoline, 8\|mercaptoquinoline, 2,3\| diaminonaphthalene, 2, 3\|dimercaptonaphthalene were tested. The 2, 3\|dimercaptonaphthalene gave better result than the other reagents. Under the optimal conditions, the concentration of 2, 3\| dimercaptonaphthalene was 5.6×10 -5 mol/L and the pH value of the solution was adjusted to 4.0 with acetate buffer solution. The excitation and emission fluorescence wavelengths of 2,3\| dimercaptonaphthalene-methylmercury complex were 512.5 nm and 500 nm(antistokes), respectively. The linear range was 0.3—80 ng/ml and the detection limit was 0.02 ng/ml. The relative standard deviation was 2.6%. The method may be applied to determine methylmercury in environmental samples, and detailed investigation is in progress.展开更多
It is found that in the presence of sulfuric acid carbohydrates condense withurea to afford the condensation products, which emit fluorescence. Under optimum conditions, thefluorescence intensities of system are propo...It is found that in the presence of sulfuric acid carbohydrates condense withurea to afford the condensation products, which emit fluorescence. Under optimum conditions, thefluorescence intensities of system are proportional to the concentrations of carbohydrates. Based onthis linear relationship, quantitative determination of kinds of carbohydrates has been made. Amongall the carbohydrates tested, the sensitivity of α-rhamnose is the highest and its limits ofdetection reaches 3. 5 * 10^(-8) mol/L. So α-rhamnose can be selectively determined in the presenceof other carbohydrates. A interaction mechanism is also discussed.展开更多
基金Project Supported by the National Natural Science Fundation of China.
文摘The Eu-Tetracycline(TC)-TOPO-sodium dodecyl sulfonate(SDS)system was studied,Experiments showed that the maximum fluorescence intensity was obtained in the pH range of 7.5-8.7 and the concentrations of Eu^(a+),TOPO and SDS are 1.0×10^(-6)mol/L,1.0×10^(-8)mol/L and 1.0×10^(-8)mol/L,respectively,This fluorescence system can be used for the determination of TC in serum and urine,Beer's Law is obeyed in the range of 2.0×10^(-8)mol/L=1.0×10^(-6)mol/L for the concentration of TC. The determination limit is 1.2×10^(-8)mol/L,The composition and the luminescence mechanism were discussed.
文摘A fluorimetric method for the determination of tyrosine in serum was proposed. The fluoriscence intensity is a linear function of tyrosine content in the range 0-1. 44ug/ml. The percentage of recovery was satisfactory.
文摘A dehydration reaction of ADM was carried out in sulfuric acid solution and an intensely fluorescent product was formed.This reaction was applied to the determination of ADM in body fluids.
文摘Methylmercury is the most toxic one among the mercury species. Many methods including atomic absorption spectrometry, atomic emission spectrometry, atomic fluorescence spectrometry, gas chromatography, high\|performance liquid chromatography, ion chromatography, and capillary electrophoresis, have been published for the determination of methylmercury. These methods have been reviewed (Li, 1997; 1993; Niu, 1991). These methods require expensive equipment and highly qualified analysts. Although fluorimetric method is sensitive, inexpensive, easy to operate, no data about determination of methylmercury by fluorimetry can be found in the literature. In order to obtain a better method for the determination of methylmercury by fluorimetry, several reagents such as 8\| hydroxyquinoline, 8\|aminoquinoline, 8\|mercaptoquinoline, 2,3\| diaminonaphthalene, 2, 3\|dimercaptonaphthalene were tested. The 2, 3\|dimercaptonaphthalene gave better result than the other reagents. Under the optimal conditions, the concentration of 2, 3\| dimercaptonaphthalene was 5.6×10 -5 mol/L and the pH value of the solution was adjusted to 4.0 with acetate buffer solution. The excitation and emission fluorescence wavelengths of 2,3\| dimercaptonaphthalene-methylmercury complex were 512.5 nm and 500 nm(antistokes), respectively. The linear range was 0.3—80 ng/ml and the detection limit was 0.02 ng/ml. The relative standard deviation was 2.6%. The method may be applied to determine methylmercury in environmental samples, and detailed investigation is in progress.
基金theNationalNaturalScienceFoundationsofChina (No .2 9875 0 15 ),theNaturalScienceFoundationofShandongProvince (No .Z97B0 10 0 2 ),andtheVisitingScholarFoundationofKeyLaboratoryinShandongUniversity
文摘It is found that in the presence of sulfuric acid carbohydrates condense withurea to afford the condensation products, which emit fluorescence. Under optimum conditions, thefluorescence intensities of system are proportional to the concentrations of carbohydrates. Based onthis linear relationship, quantitative determination of kinds of carbohydrates has been made. Amongall the carbohydrates tested, the sensitivity of α-rhamnose is the highest and its limits ofdetection reaches 3. 5 * 10^(-8) mol/L. So α-rhamnose can be selectively determined in the presenceof other carbohydrates. A interaction mechanism is also discussed.
