BACKGROUND Cerebral apoplexy patients are prone to cognitive impairment,and it is very important to choose appropriate treatment methods to improve their cognitive impairment after stroke.AIM To evaluate the effects o...BACKGROUND Cerebral apoplexy patients are prone to cognitive impairment,and it is very important to choose appropriate treatment methods to improve their cognitive impairment after stroke.AIM To evaluate the effects of enhanced external counterpulsation(EECP)in con-junction with atorvastatin on cognitive function,neurotransmitter levels,and the repair of brain tissue damage in patients with cognitive impairment due to stroke.METHODS In this retrospective study,data from 60 patients with poststroke cognitive impairment due to stroke who were treated in our hospital from February 2021 to July 2022 were analyzed and divided into a treatment group(n=30)and a control group(n=30)according to the different nursing methods applied.Patients in the treatment group received EECP in addition to atorvastatin,while those in the control group received atorvastatin alone.Mini-Mental State Examination(MMSE),Montreal Cognitive Assessment(MoCA)and activities of daily living(ADL)scale scores were compared between the two groups.Additionally,the two groups were compared in terms of serum acetylcholine(ACh),acetylcholin-esterase(AChE),nitric oxide(NO),endothelin-1(ET-1),β2-microglobulin(β2-MG),glial fibrillary acidic protein(GFAP),and visinin-like protein 1(VILIP-1)in the serum.Blood flow measurements from the anterior cerebral artery(ACA),middle cerebral artery(MCA)and posterior cerebral artery(PCA)were compared between the two groups before and after treatment,and the pulsatility index(PI)and resistance index(RI)of each artery were determined.RESULTS MMSE,MoCA,and ADL scores all improved in both groups following treatment,with the study group showing more improvement than the control group(P<0.05).After treatment,there were statistically significant increases in both ACh and NO levels,whereas decreases occurred in AChE,ET-1,β2-MG,VILIP-1,and GFAP,levels and the PI and RI of the left-ACA,right-ACA,left-MCA,right-MCA,left-PCA,and right-PCA.The study group showed greater gains in all metrics than the control group(P<0.05).CONCLUSION EECP combined with atorvastatin is effective in the treatment of cognitive impairment after stroke and can effectively improve the cognitive function,neurotransmitter levels,and brain tissue damage status of patients.展开更多
建立了超高效液相色谱-四极杆飞行时间质谱法（UPLC-Q-TOF-MS）对小鼠大脑中的多巴胺（DA）、5-羟色氨（5-HT）、二羟基苯乙酸（DOPAC）和高香草酸（HVA）4种单胺类神经递质的含量进行同时测定。样品以30%乙醇溶液（甲酸调至p H 5.0）...建立了超高效液相色谱-四极杆飞行时间质谱法（UPLC-Q-TOF-MS）对小鼠大脑中的多巴胺（DA）、5-羟色氨（5-HT）、二羟基苯乙酸（DOPAC）和高香草酸（HVA）4种单胺类神经递质的含量进行同时测定。样品以30%乙醇溶液（甲酸调至p H 5.0）匀浆处理后,冷冻离心,过滤。在优化色谱-质谱条件下,采用ESI和APCI复合源,正/负离子扫描方式进行数据采集。4种单胺类神经递质在0.5-1 000μg/L范围内呈良好线性关系,相关系数（r）大于0.999 3,方法的检出限为0.5-5.0μg/L,平均回收率为93.3%-101.8%,相对标准偏差为1.1%-3.3%。该方法具有操作简便、高效快速、回收率良好、灵敏度高、重现性好和准确度高等优点,可用于小鼠脑组织内4种单胺类神经递质的含量测定。展开更多
目的观察长期低剂量邻苯二甲酸二(2-乙基己基)酯[di(2-ethylhexyl)phthalate,DEHP]暴露及高脂喂养对小鼠脑组织氨基酸类神经递质和神经元的影响。方法选择体质量16~18 g SPF级雌性C57BL/6J小鼠,适应性喂养1周,随机分为对照组(Con组)、D...目的观察长期低剂量邻苯二甲酸二(2-乙基己基)酯[di(2-ethylhexyl)phthalate,DEHP]暴露及高脂喂养对小鼠脑组织氨基酸类神经递质和神经元的影响。方法选择体质量16~18 g SPF级雌性C57BL/6J小鼠,适应性喂养1周,随机分为对照组(Con组)、DEHP组(DEHP组)、高脂组(HFD组)、DEHP暴露联合HFD喂养组(HFD+DEHP组)。DEHP组和HFD+DEHP组小鼠经口给予50μg/kg DEHP,Con组、HFD组小鼠给予等体积溶剂,Con组、DEHP小鼠喂饲普通维持饲料,HFD组、HFD+DEHP组小鼠喂饲高脂饲料。第24周末,每组随机取8只小鼠脱臼处死,取脑组织制备匀浆,并采用高效液相色谱法检测脑组织氨基酸类神经递质水平;每组剩余4只小鼠经体内灌注固定,制备大脑冰冻切片,采用尼氏染色观察神经元的变化。结果HFD+DEHP组大脑皮层GABA高于Con组、DEHP组,HFD组、HFD+DEHP组Glu/GABA低于Con组(P<0.05);各组其他指标比较差异无统计学意义(P>0.05)。各组中脑中氨基酸类神经递质水平比较差异无统计学意义(P>0.05)。DEHP组、HFD组、HFD+DEHP组小脑Gly均高于Con组,HFD+DEHP组GABA高于Con组、DEHP组、HFD组(P<0.05);各组其他指标比较差异无统计学意义(P>0.05)。与Con组相比,DEHP组、HFD组及HFD+DEHP组小鼠大脑神经元中尼氏体颜色变淡、着色颗粒稀疏,以HFD+DEHP组最为明显。结论DEHP长期暴露和高脂饮食可破坏小鼠脑内氨基酸类神经递质的内稳态,导致神经元损伤。展开更多
基金This study was approved by the Ethics Committee of Shengjing Hospital of China Medical University.
文摘BACKGROUND Cerebral apoplexy patients are prone to cognitive impairment,and it is very important to choose appropriate treatment methods to improve their cognitive impairment after stroke.AIM To evaluate the effects of enhanced external counterpulsation(EECP)in con-junction with atorvastatin on cognitive function,neurotransmitter levels,and the repair of brain tissue damage in patients with cognitive impairment due to stroke.METHODS In this retrospective study,data from 60 patients with poststroke cognitive impairment due to stroke who were treated in our hospital from February 2021 to July 2022 were analyzed and divided into a treatment group(n=30)and a control group(n=30)according to the different nursing methods applied.Patients in the treatment group received EECP in addition to atorvastatin,while those in the control group received atorvastatin alone.Mini-Mental State Examination(MMSE),Montreal Cognitive Assessment(MoCA)and activities of daily living(ADL)scale scores were compared between the two groups.Additionally,the two groups were compared in terms of serum acetylcholine(ACh),acetylcholin-esterase(AChE),nitric oxide(NO),endothelin-1(ET-1),β2-microglobulin(β2-MG),glial fibrillary acidic protein(GFAP),and visinin-like protein 1(VILIP-1)in the serum.Blood flow measurements from the anterior cerebral artery(ACA),middle cerebral artery(MCA)and posterior cerebral artery(PCA)were compared between the two groups before and after treatment,and the pulsatility index(PI)and resistance index(RI)of each artery were determined.RESULTS MMSE,MoCA,and ADL scores all improved in both groups following treatment,with the study group showing more improvement than the control group(P<0.05).After treatment,there were statistically significant increases in both ACh and NO levels,whereas decreases occurred in AChE,ET-1,β2-MG,VILIP-1,and GFAP,levels and the PI and RI of the left-ACA,right-ACA,left-MCA,right-MCA,left-PCA,and right-PCA.The study group showed greater gains in all metrics than the control group(P<0.05).CONCLUSION EECP combined with atorvastatin is effective in the treatment of cognitive impairment after stroke and can effectively improve the cognitive function,neurotransmitter levels,and brain tissue damage status of patients.
文摘建立了超高效液相色谱-四极杆飞行时间质谱法（UPLC-Q-TOF-MS）对小鼠大脑中的多巴胺（DA）、5-羟色氨（5-HT）、二羟基苯乙酸（DOPAC）和高香草酸（HVA）4种单胺类神经递质的含量进行同时测定。样品以30%乙醇溶液（甲酸调至p H 5.0）匀浆处理后,冷冻离心,过滤。在优化色谱-质谱条件下,采用ESI和APCI复合源,正/负离子扫描方式进行数据采集。4种单胺类神经递质在0.5-1 000μg/L范围内呈良好线性关系,相关系数（r）大于0.999 3,方法的检出限为0.5-5.0μg/L,平均回收率为93.3%-101.8%,相对标准偏差为1.1%-3.3%。该方法具有操作简便、高效快速、回收率良好、灵敏度高、重现性好和准确度高等优点,可用于小鼠脑组织内4种单胺类神经递质的含量测定。
文摘目的观察长期低剂量邻苯二甲酸二(2-乙基己基)酯[di(2-ethylhexyl)phthalate,DEHP]暴露及高脂喂养对小鼠脑组织氨基酸类神经递质和神经元的影响。方法选择体质量16~18 g SPF级雌性C57BL/6J小鼠,适应性喂养1周,随机分为对照组(Con组)、DEHP组(DEHP组)、高脂组(HFD组)、DEHP暴露联合HFD喂养组(HFD+DEHP组)。DEHP组和HFD+DEHP组小鼠经口给予50μg/kg DEHP,Con组、HFD组小鼠给予等体积溶剂,Con组、DEHP小鼠喂饲普通维持饲料,HFD组、HFD+DEHP组小鼠喂饲高脂饲料。第24周末,每组随机取8只小鼠脱臼处死,取脑组织制备匀浆,并采用高效液相色谱法检测脑组织氨基酸类神经递质水平;每组剩余4只小鼠经体内灌注固定,制备大脑冰冻切片,采用尼氏染色观察神经元的变化。结果HFD+DEHP组大脑皮层GABA高于Con组、DEHP组,HFD组、HFD+DEHP组Glu/GABA低于Con组(P<0.05);各组其他指标比较差异无统计学意义(P>0.05)。各组中脑中氨基酸类神经递质水平比较差异无统计学意义(P>0.05)。DEHP组、HFD组、HFD+DEHP组小脑Gly均高于Con组,HFD+DEHP组GABA高于Con组、DEHP组、HFD组(P<0.05);各组其他指标比较差异无统计学意义(P>0.05)。与Con组相比,DEHP组、HFD组及HFD+DEHP组小鼠大脑神经元中尼氏体颜色变淡、着色颗粒稀疏,以HFD+DEHP组最为明显。结论DEHP长期暴露和高脂饮食可破坏小鼠脑内氨基酸类神经递质的内稳态,导致神经元损伤。
