The abnormal aggregation of amyloid-beta(Aβ)has been widely believed to play an important role in the pathogenesis of Alz heimer’s disease(AD),which is also recognized as one of the main biomarkers for AD diagnosis....The abnormal aggregation of amyloid-beta(Aβ)has been widely believed to play an important role in the pathogenesis of Alz heimer’s disease(AD),which is also recognized as one of the main biomarkers for AD diagnosis.The peptide sequence Lys-Leu-Val-Phe-Phe(KLVFF)is considered as the main driver of the fibrillation of Aβ,which also can be utilized to target Aβand inhibit its aggregation.In this study,KLVFF and Fmoc-KLVFF fluorescent nanoparticles were self-assembled through zinc coordination andπ-πstacking.The recognition of Aβaggregates including oligomers and fibrils by fluorescent nanoparticles can be realized through aromatic,hydrophobic,and hydrogen-bond interactions.The fluorescent nanoprobes can distinguish Aβaggregation formats and detect Aβat the limit of 1 pg/mL(S/N=3).Hence,the detection of Aβaggregates by fluorescent peptide nanoparticles has great potential for AD diagnosis and progression prediction.展开更多
基金supported by the National Natural Science Foundation of China(Nos.31900984 and 31971315)the Natural Science Basic Research Plan in Shaanxi Province of China(Nos.2019JQ-231)+3 种基金the China Postdoctoral Science Foundation(No.2018M631197)the Shaanxi Postdoctoral Science Foundation(No.2018BSHQYXMZZ42)the Fundamental Research Funds for the Central Universities(No.31020180QD063)National Undergraduate Training Programs for Innovation and Entrepreneurship(No.201810699343)。
文摘The abnormal aggregation of amyloid-beta(Aβ)has been widely believed to play an important role in the pathogenesis of Alz heimer’s disease(AD),which is also recognized as one of the main biomarkers for AD diagnosis.The peptide sequence Lys-Leu-Val-Phe-Phe(KLVFF)is considered as the main driver of the fibrillation of Aβ,which also can be utilized to target Aβand inhibit its aggregation.In this study,KLVFF and Fmoc-KLVFF fluorescent nanoparticles were self-assembled through zinc coordination andπ-πstacking.The recognition of Aβaggregates including oligomers and fibrils by fluorescent nanoparticles can be realized through aromatic,hydrophobic,and hydrogen-bond interactions.The fluorescent nanoprobes can distinguish Aβaggregation formats and detect Aβat the limit of 1 pg/mL(S/N=3).Hence,the detection of Aβaggregates by fluorescent peptide nanoparticles has great potential for AD diagnosis and progression prediction.
