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西花蓟马抗氧化酶基因FoCAT对寄主转换的响应 被引量:3
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作者 刘利 郅军锐 +2 位作者 岳文波 谢文 张涛 《山地农业生物学报》 2019年第3期22-26,共5页
为明确西花蓟马体内抗氧化酶基因FoCAT在适应不同寄主植物中的作用,利用实时荧光定量PCR技术测定了西花蓟马从菜豆豆荚分别转换到菜豆植株和蚕豆植株后,F1、F2和F3代2龄若虫和成虫体内FoCAT表达量的异同。结果表明:西花蓟马从菜豆豆荚... 为明确西花蓟马体内抗氧化酶基因FoCAT在适应不同寄主植物中的作用,利用实时荧光定量PCR技术测定了西花蓟马从菜豆豆荚分别转换到菜豆植株和蚕豆植株后,F1、F2和F3代2龄若虫和成虫体内FoCAT表达量的异同。结果表明:西花蓟马从菜豆豆荚转换到菜豆植株和蚕豆植株后,除取食蚕豆植株的F2代成虫体内FoCAT表达量和取食豆荚的没有明显区别外,其余情况下都显著降低。同一世代下FoCAT表达量在2龄若虫与成虫间差异较大。取食蚕豆植株的西花蓟马体内FoCAT的表达量均显著高于取食菜豆植株的。结果说明西花蓟马转换到新的寄主后,FoCAT迅速产生响应,其表达量与寄主的嗜食性、自身的虫态及取食的世代相关。 展开更多
关键词 西花蓟马 寄主转换 继代适应 过氧化氢酶 focat
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西洋参茎叶皂苷对局灶性脑缺血后大鼠血清中GFAP表达的影响 被引量:5
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作者 王怀刚 关利新 +4 位作者 赵鑫 鲁美君 朱丹 朱孝娟 苏云明 《中医药学报》 CAS 2011年第4期17-19,共3页
目的:观察西洋参茎叶皂苷对脑缺血大鼠血清中GFAP蛋白的影响,并进一步探讨其作用机制。方法:用线栓法制备大鼠局灶性脑缺血模型,采用酶联免疫法检测西洋参茎叶皂苷(PQS)对大鼠血清中GFAP蛋白表达的影响。结果:PQS能改善神经功能缺损的症... 目的:观察西洋参茎叶皂苷对脑缺血大鼠血清中GFAP蛋白的影响,并进一步探讨其作用机制。方法:用线栓法制备大鼠局灶性脑缺血模型,采用酶联免疫法检测西洋参茎叶皂苷(PQS)对大鼠血清中GFAP蛋白表达的影响。结果:PQS能改善神经功能缺损的症状,降低血液中GFAP蛋白的表达情况。结论:西洋参茎叶皂苷对实验动物脑缺血组织具有保护作用,其作用机理可能与星形胶质细胞的内源性保护作用有关。 展开更多
关键词 西洋参茎叶皂苷 局灶性脑缺血 GFAP
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医用诊断X线管焦点辐射对焦点MTF的影响
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作者 王淑云 王鹏程 +1 位作者 陈永创 谢银勋 《泰山医学院学报》 CAS 1990年第1期9-14,共6页
医用诊断X线管焦点的调制传递函数MTF值为评价X线管焦点成像性能的一重要参数。对具有焦点外辐射的焦点MTF计算方法以及焦点外辐射对焦点MTF的影响尚未见报道。本文结合实验研究和理论推导得到了焦点外辐射的焦点MTF计算公式,并对两X线... 医用诊断X线管焦点的调制传递函数MTF值为评价X线管焦点成像性能的一重要参数。对具有焦点外辐射的焦点MTF计算方法以及焦点外辐射对焦点MTF的影响尚未见报道。本文结合实验研究和理论推导得到了焦点外辐射的焦点MTF计算公式,并对两X线管的四个焦点进行了测量研究。 展开更多
关键词 医用X线管 焦点外辐射 焦点
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Unstable mallet fractures: a comparison between three different techniques in a multicenter study 被引量:3
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作者 Stefano Lucchina Alejandro Badia +1 位作者 Vlad Dornean Cesare Fusetti 《Chinese Journal of Traumatology》 CAS 2010年第4期195-200,共6页
Objective: Management of mallet fractures is still a matter of discussion throughout the literature. For some authors, mallet fractures involving more than 1/3 of the articular surface and palmar subluxation of the d... Objective: Management of mallet fractures is still a matter of discussion throughout the literature. For some authors, mallet fractures involving more than 1/3 of the articular surface and palmar subluxation of the distal phalanx require surgical treatment. In this study we retrospectively compared three different techniques for mallet fractures: Kirschner wire fixation with extension block pinning (EBP) of the distal interphalangeal joint, Kirschner wires used as joysticks (KWJ) and interfragmentary miniscrews for open reduction and internal fixation (ORIF). Methods: Fifty-eight mallet fractures with palmar subluxation in 58 patients were treated with the aforementioned surgical techniques. Twenty mallet fractures in 20 patients 18 to 70 years old (average 42 years) were operated upon by EBP, 16 patients 22 to 56 years old (average 56 years) were operated upon using KWJ and 22 patients 22 to 54 years old (average 36 years) received OR/F. Follow-up time was 6 to 58 months (average 21 months). The following intraoperative parameters were considered: intraoperative time, number of Kirschner wires/screws and technical problems. Postoperative parameters included work absence and complications. The radiological evaluation was based on A-P and lateral views preoperatively and interviews at follow-up time. Bone union was defined by radiological evidence of bone trabeculae crossing the fracture site on at least one view. Clinical evaluation involved range of motion (ROM) test with a goniometer. Based on these measurements, a functional Crawford score was established. Results: All fractures healed. In the KWJ group, intraoperative time was shorter and total ROM was wider (72° vs 58° and 54 °); in the ORIF group, return to work was faster (2.7 weeks vs 7.2 weeks and 6 weeks) but a little higher complication rate due to screw positioning has been found. Functional results as to total ROM, distal interphalangeal lag extension and Crawford classification were similar. Conclusions: We demonstrate the advantages of the use of the three techniques and bone consolidation in all cases with no signs ofosteoarthritis. Screw fixation is more technically demanding (longer intraoperative time and more complications) but allows earlier mobilization and faster returning to work. EBP and KWJ techniques are faster to perform with no complications but require a careful management of the pin tracts. There is no statistically significant difference as to functional results. 展开更多
关键词 Fractures bone Finger injuries Fracture focation internal Internal fixators Bone wires
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