Objective:To evaluate the effects of Crassocephalum(C.)bauchiense(Hutch)leaf aqueous extract on the biochemical markers of toxicity,oxidative stress indicators and reproductive parameters in rabbit does(Oryctolagus cu...Objective:To evaluate the effects of Crassocephalum(C.)bauchiense(Hutch)leaf aqueous extract on the biochemical markers of toxicity,oxidative stress indicators and reproductive parameters in rabbit does(Oryctolagus cuniculus)exposed to potassium dichromate.Methods:A total of 36 nulliparous and sexually mature female rabbit does,aged 8 months and weighing 2.8-3.0 kg,were divided into 6 groups of 6 animals.After mating,group T0 received distilled water(1 mL/kg body weight),while groups T0-,VC100,AE100,AE200 and AE400 were treated with 40 mg/mL/kg body weight of potassium dichromate.In addition,group VC100 received 100 mg/mL/kg body weight of vitamin C,while groups AE100,AE200 and AE400 received aqueous extract of C.bauchiense leaves at 100,200 and 400 mg/mL/kg body weight,by oral gavage for 27 days,respectively.On the 28th daypost-co?tum,animals were sacrificed.Biochemical indicators such as creatinine,urea,alanine amino tranferase,aspartate amino transferase,total protein and total cholesterol were measured by using a spectrophotometer.Follicle stimulating hormone,luteinizing hormone and progesterone were measured by enzyme-linked immuno sorbent assay.Oxidative stress markers like malondialdehyde,catalase,superoxide dismutase and total peroxidase in ovary homogenates were measured by spectrophotometer.Results:Serum concentrations of urea,creatinine,alanine aminotransferase,aspartate aminotransferase and total cholesterol were significantly higher(P<0.05),and total protein level was significantly lower in T0-group(receiving potassium dichromate only)compared to other groups.Administration of ethanolic extract of C.bauchiense significantly decreased serum concentrations of urea,creatinine,alanine aminotransferase,aspartate aminotransferase and total cholesterol,and significantly increased total protein level.However,ethanolic extract of C.bauchiensehad no significant effect on the foetotoxic characteristics.The serum concentrations of follicle stimulating hormone and luteinizing hormone were significantly(P<0.05)lower in T0-group as compared to the control group while progesterone were comparable(P>0.05)among all groups;C.bauchiense leaf extracts significantly(P<0.05)increased the level of follicle stimulating hormone,but the increase in luteinizing hormone was not significant(P>0.05).Catalase and total peroxidase activities significantly decreased(P<0.05),and malondialdehyde significantly increased in T0-group compared to other groups.The administration of C.bauchiense leaf extracts significantly(P<0.05)increased the activities of catalase and peroxidase.Conclusions:Potassium dichromate-induced oxidative stress involves in hepatotoxicity,nephrotoxicity and reprotoxicity.The aqueous extract of C.bauchiense leaves could mitigate these adverse effectsvia antioxidant properties.展开更多
文摘Objective:To evaluate the effects of Crassocephalum(C.)bauchiense(Hutch)leaf aqueous extract on the biochemical markers of toxicity,oxidative stress indicators and reproductive parameters in rabbit does(Oryctolagus cuniculus)exposed to potassium dichromate.Methods:A total of 36 nulliparous and sexually mature female rabbit does,aged 8 months and weighing 2.8-3.0 kg,were divided into 6 groups of 6 animals.After mating,group T0 received distilled water(1 mL/kg body weight),while groups T0-,VC100,AE100,AE200 and AE400 were treated with 40 mg/mL/kg body weight of potassium dichromate.In addition,group VC100 received 100 mg/mL/kg body weight of vitamin C,while groups AE100,AE200 and AE400 received aqueous extract of C.bauchiense leaves at 100,200 and 400 mg/mL/kg body weight,by oral gavage for 27 days,respectively.On the 28th daypost-co?tum,animals were sacrificed.Biochemical indicators such as creatinine,urea,alanine amino tranferase,aspartate amino transferase,total protein and total cholesterol were measured by using a spectrophotometer.Follicle stimulating hormone,luteinizing hormone and progesterone were measured by enzyme-linked immuno sorbent assay.Oxidative stress markers like malondialdehyde,catalase,superoxide dismutase and total peroxidase in ovary homogenates were measured by spectrophotometer.Results:Serum concentrations of urea,creatinine,alanine aminotransferase,aspartate aminotransferase and total cholesterol were significantly higher(P<0.05),and total protein level was significantly lower in T0-group(receiving potassium dichromate only)compared to other groups.Administration of ethanolic extract of C.bauchiense significantly decreased serum concentrations of urea,creatinine,alanine aminotransferase,aspartate aminotransferase and total cholesterol,and significantly increased total protein level.However,ethanolic extract of C.bauchiensehad no significant effect on the foetotoxic characteristics.The serum concentrations of follicle stimulating hormone and luteinizing hormone were significantly(P<0.05)lower in T0-group as compared to the control group while progesterone were comparable(P>0.05)among all groups;C.bauchiense leaf extracts significantly(P<0.05)increased the level of follicle stimulating hormone,but the increase in luteinizing hormone was not significant(P>0.05).Catalase and total peroxidase activities significantly decreased(P<0.05),and malondialdehyde significantly increased in T0-group compared to other groups.The administration of C.bauchiense leaf extracts significantly(P<0.05)increased the activities of catalase and peroxidase.Conclusions:Potassium dichromate-induced oxidative stress involves in hepatotoxicity,nephrotoxicity and reprotoxicity.The aqueous extract of C.bauchiense leaves could mitigate these adverse effectsvia antioxidant properties.
