Background: Malassezia folliculitis is an infection of the hair follicle, which commonly affects young adult, and is often misdiagnosed as truncal acne. Objectives: To shed light on different clinical, histopathologic...Background: Malassezia folliculitis is an infection of the hair follicle, which commonly affects young adult, and is often misdiagnosed as truncal acne. Objectives: To shed light on different clinical, histopathological, and microbiological aspects of Malassezia folliculitis and to be compared with truncal acne. Patients and Methods: This is a descriptive comparative cross sectional, out patient based study, which was conducted at the Department of Dermatology, Basrah Teaching Hospital from October 2008 to October 2009. Total number of 113 patients with papular and/or pustular follicular skin lesions on the trunk were enrolled in this study. They were divided according to the clinical features, Wood’s light examination, direct microscopic examination, PAS stained skin biopsy and the response to a trial of antifungal drugs into two groups: group 1: patient with Malassezia folliculitis and group 2: patient with truncal acne vulgaris. Results: Group 1: They were 53(46.9%) out of a total 113, 33(62.3%) males and 20(37.7%) females, their ages ranged from 19 - 40 years. Group 2 consisted of 60(53.1%) patients, 25(41.6%) males and 35(58.4%) females, whose ages ranged from 14 - 29 years. The ages were earlier in the acne group in comparison with MF group (p Malassezia folliculitis was more common among males than females, while in truncal acne, females predominated males. The center of the back was the predominant site among MF group affecting (90.6%) of patients with sparing the face, in contrast to truncal acne where the periphery of the back was involved in (91.7%) and the face in (75%) (p 0.0001). Wood’s light examination, skin scrapping and PAS stained skin biopsies were also positive in all patients with MF in contrast to acne group where all these tests were negative. All MF patients showed marked response to a two weeks trial of antifungal while the reverse is true for the acne patients, whereas the antibiotic was the second most common leading aggravating factor in 20 (37.7%) of cases with MF, which was significantly different from acne group (p 0.0001). Conclusion: Malassezia folliculitis should be considered in young adults with an itchy papulopustulr follicular eruptions affecting the trunk that should be differentiated from truncal acne by their characteristic clinical, histopathological features and its remarkable response to antifungal therapy.展开更多
Psoriasis is a common clinical disease, and its pathogenesis is closely related to infecting microorganisms. Studies in recent years indicated that Malassezia plays an important role in occurrence and development of p...Psoriasis is a common clinical disease, and its pathogenesis is closely related to infecting microorganisms. Studies in recent years indicated that Malassezia plays an important role in occurrence and development of psoriasis. This study provides an overview on the role of Malassezia in development of psoriasis based on three aspects, namely, clinical observation,antifungal therapy, and immunology research.展开更多
Introduction: The genus Malassezia comprises 17 species of commensal and pathogenic yeasts of homeotherms animal skin. The most common species are M. furfur, M. globosa, and M. sympodialis in humans and M. pachydermat...Introduction: The genus Malassezia comprises 17 species of commensal and pathogenic yeasts of homeotherms animal skin. The most common species are M. furfur, M. globosa, and M. sympodialis in humans and M. pachydermatis in animals. However, some publications have reported potentially serious human infections by M. pachydermatis in individuals with risk factors and the isolation of human species from domestic animals. Given the scarcity of information about their capacity for transmission between hosts and zoonotic potential, the aim of the present study was to physiologically and molecularly characterize Malassezia spp. isolates obtained from canines and their human owners. Materials and Methods: An experimental study was conducted at the Veterinary Clinic of Universidad de Ciencias Aplicadas y Ambientales of Bogotá (Colombia) from July 2015 to December 2016. Phenotypic identification and molecular characterization via the amplification of the 5.8S rDNA- ITS2 and 26S rDNA gene regions, nucleic acid sequencing, and phylogenetic analyses were performed on isolates originating from canines with otitis externa and from the skin of healthy owners compatible with Malassezia spp. Results: Eighty samples were cultured, of which 32 (40%) were suggestive of Malassezia spp. A total of 29 out of 46 (63%) isolates in canines and 3 out of 34 (9%) isolates in humans corresponded entirely with M. pachydermatis. Isolates from the canines and their owners presented similar behavior in biochemical and phospholipase activity tests, 100% molecular sequence identities, and close proximity in the phylogenetic trees. Conclusion: The isolation of M. pachydermatis from humans and their dogs with identity based on biochemical, physiological, molecular, and phylogenetic perspectives indicate the ability of this species to adapt to new hosts and its potential for zoonotic transmission. These findings contribute to knowledge of the ecology of this important fungus in human and veterinary medicine.展开更多
Background: Malassezia pachydermatis is the main causative agent of canine otitis and also of a myriad of dermatological problems in companion animals;its interaction mechanisms with host cells are still unclear. Obje...Background: Malassezia pachydermatis is the main causative agent of canine otitis and also of a myriad of dermatological problems in companion animals;its interaction mechanisms with host cells are still unclear. Objectives: To establish an in vitro infection model of M. pachydermatis-exposed RK13 cells in order to evaluate cell morphological changes as well as changes in the structure of actin filaments. Methods: Cultures of RK13 cells were infected with M. pachydermatis, alterations caused by the yeast were evaluated by optical and fluorescence microscopy. Results: M. pachydermatis adheres itself to the cell and produces the formation of multiple agglomerates that cause changes in cell morphology, formation of cell aggregates in overlays, presence of syncytia and destruction of cell culture structure. The damaged cells presented changes in the actin filaments consisting of thickening of the cell cortex and loss of stress fibers. On the other hand, the formation of perinuclear actin rings in the yeasts was observed. Conclusions: An in vitro infection model was established with M. pachydermatis and alterations in cell morphology were observed consisting of changes in the structure of the actin filaments, overgrowth of the cells and the presence of syncytia.展开更多
The authors report the clinical case of a 29-year-old Caucasian woman who presented with clinicopathological findings and a previous outbreak all suggestive of actinic superficial folliculitis, a rarely reported and p...The authors report the clinical case of a 29-year-old Caucasian woman who presented with clinicopathological findings and a previous outbreak all suggestive of actinic superficial folliculitis, a rarely reported and probably misdiagnosed phototoxic sun-induced dermatosis first described by Nieboer in 1985. Despite the exuberance of this cutaneous eruption, it is usually auto-limited, reinforcing the importance of its knowledge, for eviction of unnecessary diagnostic tests and therapies. Mechanisms of pathogenesis postulated include ultraviolet A radiation and local heat. This photodermatosis presents as monomorphic, superficial, pustular, and non-pruritic folliculitis affecting the upper body but not the face, usually arising on neck, back, shoulders and upper trunk. The follicular pustules emerge 24 - 72 h after intense exposure to heat and/or sunlight and fade spontaneously in 5 - 10 days, without scarring. This patient showed a 48-hour latency period;the number of pustules and area of the body affected were proportional to the duration of the sunlight exposure;the eruption lasted approximately 10 days. Actinic superficial folliculitis has a specific histology with follicular subcorneal sterile pustules and a mixed inflammatory infiltrate around hair follicles, probably secondary to keratinocytes and Langerhans cells involvement in the immunomodulatory actions of ultraviolet radiation. Recurrence under identical conditions may occur, after a latency period of at least 4 weeks, but usually about 1 year. Actinic superficial folliculitis and related follicular conditions are probably underdiagnosed and subsequently there is insufficient scientific information available to clinicians. Being familiar with these entities is of the utmost importance, since it can be crucial for their management.展开更多
AIM:To verify curative effect of He Ne laser on folliculitis keloidalis nuchae.METHODS:103 cases with folliculitis keloidalis nuchae were divided into the treatment group(73 cases) and the control group(30 cases). Loc...AIM:To verify curative effect of He Ne laser on folliculitis keloidalis nuchae.METHODS:103 cases with folliculitis keloidalis nuchae were divided into the treatment group(73 cases) and the control group(30 cases). Local application and oral antiauxins was used in the two groups,He Ne laser radiation was used in the treatment group and then curative effect 15 days later were compared.RESULTS:The cure rats in the treatment group and control group were respectively 71.2%(52/73) and 30%(9/30)(P< 0.01) while curative days were respectively 9.2 days and 11.9 days (P< 0.01).CONCLUSION:In treatment of folliculitis Keloidalis nuchae,the application of He Ne Laser radiation can effectively reduce the course,pain and side effect of treatment,and raise the curative effect.展开更多
The prevalence rate of acne in China is continuously increasing in adolescents, young and middle-aged adults. In many cases, those who have refractory inflammatory papules and pustules on the face and mandibular have ...The prevalence rate of acne in China is continuously increasing in adolescents, young and middle-aged adults. In many cases, those who have refractory inflammatory papules and pustules on the face and mandibular have received long-term antibiotic therapy. Some severe cases have had isotretinoin treatment and even corficosteroid application by either topical or oral administration. The curative effect has not been prominent in these cases, even with combined treatment including antimicrobials and/or isotretinoin and Chinese patent medicines.展开更多
To the Editor： A 64-year-old Chinese man had a 12-month history ofnonmetastatic primary scalp angiosarcoma [Figure 1a] that had been confirmed by skin biopsy [Figure I el. Immunohistochemical examination showed CD31-...To the Editor： A 64-year-old Chinese man had a 12-month history ofnonmetastatic primary scalp angiosarcoma [Figure 1a] that had been confirmed by skin biopsy [Figure I el. Immunohistochemical examination showed CD31-, CD34-, and D2-40-positive tumor cells [Figure lf-lh]. Extensive local excision and placement of a free skin graft was performed in December 2016.展开更多
Introduction Folliculitis decalvans (FD) was first described by Quinquaud in 1888, and its clinical features consist of follicular pustules and papules with hemorrhagic crusting and resultant alopecic scarring patches...Introduction Folliculitis decalvans (FD) was first described by Quinquaud in 1888, and its clinical features consist of follicular pustules and papules with hemorrhagic crusting and resultant alopecic scarring patches(1)The early histopathologic findings of this disease show that dense perifollicular inflammatory infiltrates are mostly consisted of neutrophils, and in later stages with follicular rupture, lymphocytes, histiocytes, and plasma cells are seen, as well as perifollicular and interstitial dermal fibrosis(1)In 1978, Smith and Sanderson first described a rare folliculitis on scalp that patches of scarring alopecia with multiple hair tufts emerging from dilated follicular orifices(2)Tufting of hair was caused by clustering of adjacent follicular units due to a fibrosing process and to retention of telogen hairs within a dilated follicular orifice(3)Here, we descripe this rare FD case with keloid and tufted hair, which may demonstrate the effects of keloid in the process of FD with tufted hair.展开更多
文摘Background: Malassezia folliculitis is an infection of the hair follicle, which commonly affects young adult, and is often misdiagnosed as truncal acne. Objectives: To shed light on different clinical, histopathological, and microbiological aspects of Malassezia folliculitis and to be compared with truncal acne. Patients and Methods: This is a descriptive comparative cross sectional, out patient based study, which was conducted at the Department of Dermatology, Basrah Teaching Hospital from October 2008 to October 2009. Total number of 113 patients with papular and/or pustular follicular skin lesions on the trunk were enrolled in this study. They were divided according to the clinical features, Wood’s light examination, direct microscopic examination, PAS stained skin biopsy and the response to a trial of antifungal drugs into two groups: group 1: patient with Malassezia folliculitis and group 2: patient with truncal acne vulgaris. Results: Group 1: They were 53(46.9%) out of a total 113, 33(62.3%) males and 20(37.7%) females, their ages ranged from 19 - 40 years. Group 2 consisted of 60(53.1%) patients, 25(41.6%) males and 35(58.4%) females, whose ages ranged from 14 - 29 years. The ages were earlier in the acne group in comparison with MF group (p Malassezia folliculitis was more common among males than females, while in truncal acne, females predominated males. The center of the back was the predominant site among MF group affecting (90.6%) of patients with sparing the face, in contrast to truncal acne where the periphery of the back was involved in (91.7%) and the face in (75%) (p 0.0001). Wood’s light examination, skin scrapping and PAS stained skin biopsies were also positive in all patients with MF in contrast to acne group where all these tests were negative. All MF patients showed marked response to a two weeks trial of antifungal while the reverse is true for the acne patients, whereas the antibiotic was the second most common leading aggravating factor in 20 (37.7%) of cases with MF, which was significantly different from acne group (p 0.0001). Conclusion: Malassezia folliculitis should be considered in young adults with an itchy papulopustulr follicular eruptions affecting the trunk that should be differentiated from truncal acne by their characteristic clinical, histopathological features and its remarkable response to antifungal therapy.
文摘Psoriasis is a common clinical disease, and its pathogenesis is closely related to infecting microorganisms. Studies in recent years indicated that Malassezia plays an important role in occurrence and development of psoriasis. This study provides an overview on the role of Malassezia in development of psoriasis based on three aspects, namely, clinical observation,antifungal therapy, and immunology research.
文摘Introduction: The genus Malassezia comprises 17 species of commensal and pathogenic yeasts of homeotherms animal skin. The most common species are M. furfur, M. globosa, and M. sympodialis in humans and M. pachydermatis in animals. However, some publications have reported potentially serious human infections by M. pachydermatis in individuals with risk factors and the isolation of human species from domestic animals. Given the scarcity of information about their capacity for transmission between hosts and zoonotic potential, the aim of the present study was to physiologically and molecularly characterize Malassezia spp. isolates obtained from canines and their human owners. Materials and Methods: An experimental study was conducted at the Veterinary Clinic of Universidad de Ciencias Aplicadas y Ambientales of Bogotá (Colombia) from July 2015 to December 2016. Phenotypic identification and molecular characterization via the amplification of the 5.8S rDNA- ITS2 and 26S rDNA gene regions, nucleic acid sequencing, and phylogenetic analyses were performed on isolates originating from canines with otitis externa and from the skin of healthy owners compatible with Malassezia spp. Results: Eighty samples were cultured, of which 32 (40%) were suggestive of Malassezia spp. A total of 29 out of 46 (63%) isolates in canines and 3 out of 34 (9%) isolates in humans corresponded entirely with M. pachydermatis. Isolates from the canines and their owners presented similar behavior in biochemical and phospholipase activity tests, 100% molecular sequence identities, and close proximity in the phylogenetic trees. Conclusion: The isolation of M. pachydermatis from humans and their dogs with identity based on biochemical, physiological, molecular, and phylogenetic perspectives indicate the ability of this species to adapt to new hosts and its potential for zoonotic transmission. These findings contribute to knowledge of the ecology of this important fungus in human and veterinary medicine.
文摘Background: Malassezia pachydermatis is the main causative agent of canine otitis and also of a myriad of dermatological problems in companion animals;its interaction mechanisms with host cells are still unclear. Objectives: To establish an in vitro infection model of M. pachydermatis-exposed RK13 cells in order to evaluate cell morphological changes as well as changes in the structure of actin filaments. Methods: Cultures of RK13 cells were infected with M. pachydermatis, alterations caused by the yeast were evaluated by optical and fluorescence microscopy. Results: M. pachydermatis adheres itself to the cell and produces the formation of multiple agglomerates that cause changes in cell morphology, formation of cell aggregates in overlays, presence of syncytia and destruction of cell culture structure. The damaged cells presented changes in the actin filaments consisting of thickening of the cell cortex and loss of stress fibers. On the other hand, the formation of perinuclear actin rings in the yeasts was observed. Conclusions: An in vitro infection model was established with M. pachydermatis and alterations in cell morphology were observed consisting of changes in the structure of the actin filaments, overgrowth of the cells and the presence of syncytia.
文摘The authors report the clinical case of a 29-year-old Caucasian woman who presented with clinicopathological findings and a previous outbreak all suggestive of actinic superficial folliculitis, a rarely reported and probably misdiagnosed phototoxic sun-induced dermatosis first described by Nieboer in 1985. Despite the exuberance of this cutaneous eruption, it is usually auto-limited, reinforcing the importance of its knowledge, for eviction of unnecessary diagnostic tests and therapies. Mechanisms of pathogenesis postulated include ultraviolet A radiation and local heat. This photodermatosis presents as monomorphic, superficial, pustular, and non-pruritic folliculitis affecting the upper body but not the face, usually arising on neck, back, shoulders and upper trunk. The follicular pustules emerge 24 - 72 h after intense exposure to heat and/or sunlight and fade spontaneously in 5 - 10 days, without scarring. This patient showed a 48-hour latency period;the number of pustules and area of the body affected were proportional to the duration of the sunlight exposure;the eruption lasted approximately 10 days. Actinic superficial folliculitis has a specific histology with follicular subcorneal sterile pustules and a mixed inflammatory infiltrate around hair follicles, probably secondary to keratinocytes and Langerhans cells involvement in the immunomodulatory actions of ultraviolet radiation. Recurrence under identical conditions may occur, after a latency period of at least 4 weeks, but usually about 1 year. Actinic superficial folliculitis and related follicular conditions are probably underdiagnosed and subsequently there is insufficient scientific information available to clinicians. Being familiar with these entities is of the utmost importance, since it can be crucial for their management.
文摘AIM:To verify curative effect of He Ne laser on folliculitis keloidalis nuchae.METHODS:103 cases with folliculitis keloidalis nuchae were divided into the treatment group(73 cases) and the control group(30 cases). Local application and oral antiauxins was used in the two groups,He Ne laser radiation was used in the treatment group and then curative effect 15 days later were compared.RESULTS:The cure rats in the treatment group and control group were respectively 71.2%(52/73) and 30%(9/30)(P< 0.01) while curative days were respectively 9.2 days and 11.9 days (P< 0.01).CONCLUSION:In treatment of folliculitis Keloidalis nuchae,the application of He Ne Laser radiation can effectively reduce the course,pain and side effect of treatment,and raise the curative effect.
文摘The prevalence rate of acne in China is continuously increasing in adolescents, young and middle-aged adults. In many cases, those who have refractory inflammatory papules and pustules on the face and mandibular have received long-term antibiotic therapy. Some severe cases have had isotretinoin treatment and even corficosteroid application by either topical or oral administration. The curative effect has not been prominent in these cases, even with combined treatment including antimicrobials and/or isotretinoin and Chinese patent medicines.
文摘To the Editor： A 64-year-old Chinese man had a 12-month history ofnonmetastatic primary scalp angiosarcoma [Figure 1a] that had been confirmed by skin biopsy [Figure I el. Immunohistochemical examination showed CD31-, CD34-, and D2-40-positive tumor cells [Figure lf-lh]. Extensive local excision and placement of a free skin graft was performed in December 2016.
文摘Introduction Folliculitis decalvans (FD) was first described by Quinquaud in 1888, and its clinical features consist of follicular pustules and papules with hemorrhagic crusting and resultant alopecic scarring patches(1)The early histopathologic findings of this disease show that dense perifollicular inflammatory infiltrates are mostly consisted of neutrophils, and in later stages with follicular rupture, lymphocytes, histiocytes, and plasma cells are seen, as well as perifollicular and interstitial dermal fibrosis(1)In 1978, Smith and Sanderson first described a rare folliculitis on scalp that patches of scarring alopecia with multiple hair tufts emerging from dilated follicular orifices(2)Tufting of hair was caused by clustering of adjacent follicular units due to a fibrosing process and to retention of telogen hairs within a dilated follicular orifice(3)Here, we descripe this rare FD case with keloid and tufted hair, which may demonstrate the effects of keloid in the process of FD with tufted hair.
