Design and Research of High-throughput Automation System for Food Testing Laboratory

In the process of food testing,human operation is an important variable affecting the experimental results.In order to reasonably avoid the influence of human subjective operation behavior on the accuracy of detection results,the laboratory information management system was used as the information platform to design a high-throughput laboratory automation pre-treatment system based on the deep integration of mechanical principles,visual analysis,high-speed conduction,intelligent storage and other technical systems.The experimental results showed that the system could shorten the sample circulation cycle,effectively improve the laboratory biosafety,and meet the requirements of high-throughput processing of samples.

Rapid testing methods for food contaminants and toxicants

Food safety is one of the major concerns in every country regardless of the economic and social development. The frequent occurrence of food scandals in the world has led the Chinese government to implement several strategies to fortify the food supply system to a high food safety standard. This relies heavily on laboratory testing services but conventional methods for detection of food contaminants and toxicants are limited by sophisticated sample preparation procedures, long analysis time, large instruments and professional personnel to meet the increasing demands. In this review, we have incorporated most of the current and potential rapid detection methods for many notorious food contaminants and toxicants including microbial agents, toxic ions, pesticides, veterinary drugs and preservatives, as well as detection of genetically modified food genes and adulterated edible oil. Development of rapid, accurate, easy-to-use and affordable testing methods could urge food handlers and the public to actively screen for food contaminants and toxicants instead of passively relying on monitoring by the government examination facility. This review also provides several recommendations including how to encourage the public to engage in the food safety management system and provide optimal education and financial assistance that may improve the current Chinese food safety control system.

Mode Innovation of School-Enterprise Cooperation on Food Safety Testing Laboratory Reform

In view of the difficulties about school-enterprise cooperation on food safety testing laboratory reform,the current training program,teaching methods and teaching forms are not suitable for the new platform.This paper provides new ideas and modes to solve these problems,such as integrating the resource elements between school and enterprise,establishing new platforms with the help of external force and innovating the cooperation modes to improve the laboratory,which can fully serve teaching,scientific research and enterprise production.

Food intolerance and skin prick test in treated and untreated irritable bowel syndrome

AIM： To correlate the clinical features of treated and untreated patients with irritable bowel syndrome （IBS） to the results of skin prick test （SPT） for food and inhalant allergens. METHODS： We recruited 105 subjects to form three different target groups： treated group （n=44） undergoing treatment for IBS, untreated group （n =31） meeting the Rome Ⅱ criteria without treatment for IBS, control group （n = 30） with no IBS symptoms. RESULTS： SPT results were different among the three groups in which SPT was positive in 17 （38.6%） treated patients, in 5 （16.1%） untreated patients and in 1 （3.3%） control （P〈0.01）. The number of positive SPTs was greater in the IBS group than in the control group （P〈 0.001）. The number of positive food SPTs was higher in the treated IBS group than in the untreated IBS group （P= 0.03）. CONCLUSION： Positive food SPT is higher in IBS patients than in controls.

Feasibility Study for Applying Spectral Imaging for Wheat Grain Authenticity Testing in Pasta

Authentication of pasta is currently determined using molecular biology-based techniques focusing on DNA as the target analyte. Whilst proven to be effective, these approaches can be criticised as being destructive, time consuming, and requiring specialist instrument training. Advances in the field of multispectral imaging (MSI) and hyperspectral imaging (HSI) have facilitated the development of compact imaging platforms with the capability to rapidly differentiate a range of materials (inclusive of grains and seeds) based on surface colour, texture and chemical composition. This preliminary investigation evaluated the applicability of spectral imaging for identification and quantitation of durum wheat grain samples in relation to pasta authenticity. MSI and HSI were capable of rapidly distinguishing between durum wheat and adulterant common wheat cultivars and assigning percentage adulteration levels characterised by low biases and good repeatability estimates. The results demonstrated the potential for spectral imaging based seed/grain adulteration testing to augment existing standard molecular approaches for food authenticity testing.

Improving quality of life in self-reported gastrointestinal disorders: An open trial of a food elimination diet guided by the ImmunoBloodprint food sensitivity test

Purpose: Irritable bowel syndrome (IBS) and gastrointestinal (GI) disorders are genuine health problems that have serious consequences on a patient’s quality of life (QoL). IBS is a functional disorder characterized by chronically recurring symptoms, including abdominal pain or discomfort, altered stool frequency and consistency, and abdominal bloating in the absence of structural or biochemical abnormalities. Studies have indicated that symptoms and disability related to IBS and GI disorders correlate with QoL measures. Additionally, those who respond to therapy have an improvement in QoL. Therefore, we explored an open trial of a food elimination diet guided by the ImmunoBloodprint food sensitivity test and its effect on QoL in patients self-reporting an unresolved GI disorder. Methods: Eighty-four subjects aged 18 and above participated in the study. Subjects eliminated all IgG-mediated reactive foods from their diet for 90 days. QoL was assessed with the Irritable Bowel Syndrome-36 (IBS-36) and the Medical Outcomes Study Short Form-36 (SF-36) at baseline and 30-, 60-, and 90-day follow-up. The data were analyzed with linear mixed models. Results: Subjects had statistically significant improvements in all indicators of QoL according to the IBS-36 and SF-36 from baseline to 90- day follow-up. Conclusions: Subjects with unresolved self-reported GI disorders were able to improve all indicators of QoL in response to eliminating IgG- reactive foods from the diet. Given the inconvenience of keeping track of foods and symptoms to eliminate intolerant foods, a food sensitivity test combined with an elimination diet may be an effective method for improving subjective markers of QoL and health.

Food Additives-Ascorbic Acid Quality Assay Test Intelligent Management

In this paper, based on the analysis and test methods of national standards （GB 14754-2010） and chemical analysis and test items carried out by chemical enterprises, a set of automatic processing of quality analysis test data of ascorbic acid products was developed by using access database technology and Visual Basic programming language system, and its stability was investigated. The results show that the software can manage intelligently all aspects of the quality analysis and test of ascorbic acid products, uploading timely the data and results of the analysis and inspection to the network and saving it for users, enterprises and quality management, which set up a network of information sharing platform to ensure the authenticity and reliability of measurement results, improving greatly the speed of data processing, saving valuable time, reducing production costs with good economic efficiency and social benefit. It has practical value for ascorbic acid quality analysis test data processing automatically the results of the implementation of intelligent management.

Personal glucose meters coupled with signal amplification technologies for quantitative detection of non-glucose targets:Recent progress and challenges in food safety hazards analysis

Ensuring food safety is paramount worldwide.Developing effective detection methods to ensure food safety can be challenging owing to trace hazards,long detection time,and resource-poor sites,in addition to the matrix effects of food.Personal glucose meter(PGM),a classic point-of-care testing device,possesses unique application advantages,demonstrating promise in food safety.Currently,many studies have used PGM-based biosensors and signal amplification technologies to achieve sensitive and specific detection of food hazards.Signal amplification technologies have the potential to greatly improve the analytical performance and integration of PGMs with biosensors,which is crucial for solving the challenges associated with the use of PGMs for food safety analysis.This review introduces the basic detection principle of a PGM-based sensing strategy,which consists of three key factors:target recognition,signal transduction,and signal output.Representative studies of existing PGM-based sensing strategies combined with various signal amplification technologies(nanomaterial-loaded multienzyme labeling,nucleic acid reaction,DNAzyme catalysis,responsive nanomaterial encapsulation,and others)in the field of food safety detection are reviewed.Future perspectives and potential opportunities and challenges associated with PGMs in the field of food safety are discussed.Despite the need for complex sample preparation and the lack of standardization in the field,using PGMs in combination with signal amplification technology shows promise as a rapid and cost-effective method for food safety hazard analysis.

电化学技术在食品检测中的应用研究进展

电化学技术利用待测物质发生反应产生的电化学信号实现其检测,因其操作简单、成本低、精密度及灵敏度高等优点在食品检测分析中的应用备受关注,并取得了许多研究进展。本文从电化学检测方法、类型、基本原理和在食品检测领域的研究现状方面进行了综述和分析,并对其应用发展前景进行展望,为促进电化学技术在食品检测分析领域的应用提供参考,以满足食品快速检测分析的发展需求。

应用于食品检测领域的分子即时检测技术研究进展

食品安全问题关系人类民生。为有效预防和控制食品安全风险,减少食品安全经济损失,迫切需要开发出针对食源性病原体、食物掺假、转基因作物的高特异性、高敏感性、快速的核酸检测技术。即时检测(point-of-care testing,POCT)作为一种新兴的快速检测手段,由于其检测速度快、操作简单、现场检查等特点,在食品检测领域应用广泛。本文首先介绍了POCT技术的发展历史,而后根据食品安全事件的特点,从食源性致病菌、食物掺假、转基因作物3个方向分析了食品检测的主要对象,阐述了分子POCT技术在食品检测领域的应用现状,最后对应用于食品检测领域的分子POCT技术存在的问题和解决办法进行了分析总结,对食品检测分子POCT技术的发展方向进行了展望。本文有助于进一步了解分子POCT技术在食品检测中的应用,并为分子POCT技术在食品快速检测中的研究和应用提供参考。

微生物快速检测技术在食品安全检测中的应用

在食品安全领域,微生物检测是确保食品质量和消费者健康的关键环节。随着食品工业的快速发展和消费者对食品安全要求的提高,传统的微生物检测方法已难以满足现代食品安全检测的要求。因此,快速检测技术成为了食品微生物检测领域的重要发展趋势。微生物快速检测技术因操作简便、检测速度快、灵敏度高和准确性好等特点在食品安全检测中表现出了巨大的应用潜力。文章对微生物快速检测技术在食品安全检测中的应用展开探讨,以期为相关人员提供参考。

高职“食品理化检验技术”课程思政建设研究

“课程思政”作为一种新兴的教学理念成为近年来高校课程改革的热点。针对“食品理化检验技术”课程建设中存在的问题,结合课程特点和食品检测岗位职业素养需求,从提升专业课教师思政意识和育人能力、课程思政元素的挖掘与融入、课程思政教学融合信息技术及课程思政评价四个方面阐述“食品理化检验技术”课程思政的实施途径,以此发挥专业课堂教学协同育人的作用,实现价值塑造、知识传授和能力培养三者相融,落实立德树人的根本任务,为更多的职业院校开展同类课程教学改革提供建议和参考。

2020—2023年山东省泰安市豆腐中重金属含量调查分析

目的:了解山东省泰安市豆腐中重金属含量,为预防控制重金属的急慢性中毒提供科学依据。方法:采用电感耦合等离子体质谱法检测2020—2023年山东省泰安市480份豆腐中6种元素含量,分析不同元素含量水平和趋势。结果:480份豆腐中均检出了一种或多种元素,但超标率相对较低,为8.54%(41/480)。不同年份、不同采样点之间各元素的超标率差异均无统计学意义(P>0.05)。不同种类豆腐之间各元素的超标率差异具有统计学意义(P<0.05),卤水豆腐和石膏豆腐超标率高于酸浆豆腐和内酯豆腐。不同元素的检出率和超标率有明显差别,铅和镉的检出率和超标率高于其他4种元素。结论:2020—2023年泰安市市售豆腐中,卤水豆腐和石膏豆腐中铅和镉的超标率较高。

浅析食品微生物检验技术在食品安全中的应用

随着分子生物学、生物信息学和纳米技术等现代科技的发展,食品微生物检验技术正朝着更高的灵敏度、更快的检测速度迅速发展,大幅提高了食品安全监管的效率,为食品工业的可持续发展提供了技术支撑。基于此,本文分析了食品微生物检验技术在食品安全中的应用,以供参考。

食物过敏诊断过程中存在问题及对策

由于发病机制复杂、临床表现多样化,且缺乏特异性生物标志物,使得食物过敏的诊断极具挑战性。口服食物激发试验是确诊食物过敏的金标准。食物过敏的规范诊治需要临床医生理解过敏相关的基础知识,便于正确选择诊断方法并合理解释检查结果。

食品检测中近红外光谱分析技术的应用研究

为了实现对食品成分、品质等方面的准确检测,为食品安全监管和质量控制提供有效的技术支持,引入近红外光谱分析技术,开展了该项技术在食品检测中的应用研究。通过样品制备、选择光谱采集设备、设置采集参数,采集食品近红外光谱,引入标准正态变量变换算法和自适应滤波算法,预处理采集的光谱数据,基于预处理后的数据,结合主成分分析法,构建食品成分特征与近红外光谱数据之间的数学模型,实现近红外光谱分析技术在食品检测的应用设计。实验结果表明,提出的研究应用后,食品成分检测结果与真实值更加接近,检测均方根误差较小,食品检测的准确性得到了显著提升。

食品中菌落总数的测定分析

随着我国社会经济的持续发展,国民的物质生活水平显著提升,对食品安全的关注度也日益提高。在此背景下,相关职能部门越来越重视食品中微生物的检测,并将食品中菌落总数测定确立为一项强制性任务,以保障公众的食品安全。对此,文章首先阐述了菌落总数测定的定义和方法,然后分析了食品菌落总数测定中常见的问题,并探讨了主要的测定思路及质量控制措施,以期为保障食品中菌落总数测定工作的顺利开展提供有益参考。

基于改进ELM和计算机视觉的核桃缺陷检测

目的:解决现有食品生产企业在核桃缺陷检测中存在的准确性低和效率差等问题。方法:提出一种结合改进极限学习机和计算机视觉的核桃缺陷快速无损检测方法。通过计算机视觉采集核桃大部分表面图像信息,通过高斯滤波对图像进行预处理,通过迭代和保留信息变量法对颜色和纹理特征进行优化,最后,通过改进蝴蝶算法对极限学习机参数(随机权重和偏差)进行优化,实现核桃缺陷快速无损检测,并对所提缺陷检测方法的性能进行验证。结果:试验方法可以实现核桃多种缺陷的有效判别。与常规方法相比,试验方法在核桃缺陷检测中具有更优的检测准确率和效率,检测准确率>98.00%,平均检测时间<9.00 ms。结论:将智能算法和机器视觉技术相结合可以实现核桃缺陷的快速无损检测。

食品检验检测中样品管理和控制存在的问题及对策

规范食品检验检测流程,加强样品管理和控制,不仅能够保证检测结果的准确性和可靠性,还有助于提高食品安全监管的效率和水平。食品安全问题一直是社会关注的焦点,食品检验检测作为食品安全监管的关键技术手段,其结果的准确性和可靠性至关重要。本文在分析样品选择、样品保存、检测设备及方法、样品污染和样品追溯性等问题的基础上,提出针对性的解决对策,旨在提高检验检测结果可信度,守护民众饮食安全。

一起不明原因食源性疾患流行病调查及实验室结果分析

目的:查明一起食物中毒事件暴发的原因和可疑危险因素,提出有针对性的防控措施和建议,防止类似事件再次发生。方法:收集整理暴发疫情调查处理资料,包括病例搜索及个案调查、现场卫生学调查、实验室检测等资料,通过实验室检测数据和流行病学特征综合分析得出事件原因。结果:现场流行病学调查发现有储存不当的食品,剩余食品和环境涂抹样本中检出有金黄色葡萄球菌和蜡样芽孢杆菌,二层男厕所厕坑及门把手检出金黄色葡萄球菌,肠毒素A阳性,患病学生桌面及座椅、二层和三层男厕所的厕坑及门把手检出I型诺如病毒。结论:本次食源性腹泻疫情为不洁饮食和诺如病毒叠加作用引起。