The present work aims to study the influence of antioxidants activity of lion’s foot (Alchemilla vulgaris) leaves at different concentrations to give more protection against chronic liver disease. Results indicated t...The present work aims to study the influence of antioxidants activity of lion’s foot (Alchemilla vulgaris) leaves at different concentrations to give more protection against chronic liver disease. Results indicated that dried lion’s foot leaves had rich in total polyphenolic and flavonoids content (395.65 and 183.10 mg/100g, respectively). These results were reflected to the antioxidant activity (DPPH);it’s noticed that the antioxidant activity of dried lion’s foot leaves was high (131.74%). The major polyphenolic components were benzoic acid (1084.63 ppm) followed by ellagic acid, catechol, and catechin (614.16, 580.54, and 566.53 ppm, respectively) then salicylic acid and protocatechuic acid (479.71 and 444.43 ppm, respectively). On the same trend, flavonoids fractions indicated the highest content in luteo-6-arabinase 8-glucose, apig. 6-rhamnase 8-glucose, acatein, narengin and luteolin (40.01;15.04;8.07;6.64 and 6.42 ppm, respectively). Fifty-six male albino rats were used in biological experiments. Rats fed on basal diet for two weeks before the performance of the experiment. At the beginning, rats divided into eight main group were fed on diets for 45 days as follows: Negative control group (first group) was fed on basal diet. Forty nine rats were fed on basal diet and induced by CCl4, in paraffin oil (50% v/v, 2 ml/Kg) twice weeks subcutaneous injection to induce chronic damage in the liver, then divided into 7 groups numbered from group 2 to group 8. Positive control group rats fed on basal diet till final experiment (second group). Group 3 and 4 rats treated with 50 and 100 ppm ethanolic leaves extracts, respectively. Also, group 5 and 6 treated with 50 and 100 ppm aqueous leaves extracts, respectively. All extracts were fed on orally every day. While, rats in group 7 treated with 1% and 2% dried lion’s foot leaves. At the end of the experimental period, serums were collected to determine liver and renal functions. The liver was removed surgically for histopathological observation. The results revealed that CCl4 intoxication impaired liver function. Serum AST, ALT, ALP and total bilirubin levels were elevated by CCl4 administration, while significant decreasing was noticed in serum albumin in CCl4 group. Histopathologically, CCl4 caused congestion of central vain, fatty change of hepatocytes, and focal inflammatory cells in filtration. Treatment with lion’s foot with different forms and concentration attenuated these adverse effects and markedly ameliorated histopathological and biochemical alterations caused by CCl4 especially with 2% powder and 100 ppm ethanol extract administration. Therefore, the results of this study concluded that lion’s foot can be proposed to protect hepatotoxicity induced by CCl4 in rats. The results also revealed that the hepatoprotection effect of lion’s foot may be attributed to its antioxidant contents and free radical scavenger effect.展开更多
目的探究高压氧联合硫辛酸治疗糖尿病足的临床疗效。方法选取2020年5月至2022年5月河南省人民医院豫东分院收治的92例糖尿病足患者作为研究对象,按照不同治疗方法将其分为观察组(32例)、对照组(30例)和空白组(30例)。观察组患者在常规...目的探究高压氧联合硫辛酸治疗糖尿病足的临床疗效。方法选取2020年5月至2022年5月河南省人民医院豫东分院收治的92例糖尿病足患者作为研究对象,按照不同治疗方法将其分为观察组(32例)、对照组(30例)和空白组(30例)。观察组患者在常规治疗的基础上行高压氧联合硫辛酸治疗,对照组患者在常规治疗的基础上单纯行硫辛酸治疗,空白组患者单纯行常规治疗,对比观察3组患者临床疗效、血糖相关指标及血管功能相关因子水平与踝肱指数。结果治疗4周后,3组患者临床疗效对比,观察组明显优于空白组(Z=-2.429,P=0.015),而观察组与对照组以及对照组与空白组间无明显差异(Z=-1.415、-1.299,P=0.157、0.194)。治疗4周后,3组患者空腹血糖(FBG)、餐后2 h血糖(2 h PBG)、糖化血红蛋白(HbAlc)水平对比,观察组均明显低于空白组(q=6.574、6.162、6.339,P均<0.001),而观察组与对照组以及对照组与空白组间无明显差异(FBG:q=3.310、3.213,P=0.055、0.065;2 h PBG:q=2.840、3.270,P=0.116、0.059;HbAlc:q=3.230、3.059,P=0.063、0.083);3组患者血清内皮素(ET)水平对比,观察组明显低于对照组与空白组(q=3.443、6.630,P=0.044、P<0.001),而对照组与空白组间无明显差异(q=3.137,P=0.074);3组患者血清降钙素基因相关肽(CGRP)、血管内皮生长因子(VEGF)水平对比,观察组均明显高于对照组与空白组(CGRP:q=3.491、6.883,P=0.041、P<0.001;VEGF:q=3.467、6.636,P=0.042、P<0.001),而对照组与空白组间无明显差异(q=3.339、2.851,P=0.053、0.114)。治疗4周后,3组患者踝肱指数对比,观察组明显高于对照组与空白组(q=3.947、7.330,P=0.018、P<0.001),而对照组与空白组间无明显差异(q=3.330,P=0.054)。结论高压氧联合硫辛酸治疗糖尿病足,有助于控制血糖水平、改善血管内皮功能及下肢血管病变程度,提高治疗效果,临床推广价值较高。展开更多
No recommended guidelines currently exist for the therapeutic concentration or dose of botulinum toxin type A （BTXA） injected into the muscle to treat limb spasticity. Therefore, in this randomized controlled trial,...No recommended guidelines currently exist for the therapeutic concentration or dose of botulinum toxin type A （BTXA） injected into the muscle to treat limb spasticity. Therefore, in this randomized controlled trial, we explored the safety and efficacy of two concentrations and two doses of BTXA in the treatment of spastic toot after stroke to optimize this treatment in these patients. Eligible patients （n = 104） were randomized into four groups. The triceps surae and tibialis posterior on the affected side were injected with BTXA at one of two doses （200 U or 400 U） and two concentrations （50 U/mL or 100 U/mL）. 2he following assessments were conducted before as well as 4 days and 1, 2, 4, and 12 weeks after treatment： spasticity, assessed using the modified Ashworth scale; basic functional mobility, assessed using a timed up and go test; pace, assessed using a 10-meter timed walking test; and the ability to walk, assessed using Holden＇s graded scale and a visual analog scale. The reported results are based on the 89 patients that completed the study. We found significant differences for the two doses and concentrations of BTXA to improve the ability of patients to walk independently, with the high-dose/low-concentration combination providing the best effect. Onset and duration of the ameliorating effects of BTXA were 4-7 days and 12 weeks, respectively. Thus, BTXA effectively treated foot spasms after stroke at an optimal dose of 400 U and concentration of 50 U/mL.展开更多
文摘The present work aims to study the influence of antioxidants activity of lion’s foot (Alchemilla vulgaris) leaves at different concentrations to give more protection against chronic liver disease. Results indicated that dried lion’s foot leaves had rich in total polyphenolic and flavonoids content (395.65 and 183.10 mg/100g, respectively). These results were reflected to the antioxidant activity (DPPH);it’s noticed that the antioxidant activity of dried lion’s foot leaves was high (131.74%). The major polyphenolic components were benzoic acid (1084.63 ppm) followed by ellagic acid, catechol, and catechin (614.16, 580.54, and 566.53 ppm, respectively) then salicylic acid and protocatechuic acid (479.71 and 444.43 ppm, respectively). On the same trend, flavonoids fractions indicated the highest content in luteo-6-arabinase 8-glucose, apig. 6-rhamnase 8-glucose, acatein, narengin and luteolin (40.01;15.04;8.07;6.64 and 6.42 ppm, respectively). Fifty-six male albino rats were used in biological experiments. Rats fed on basal diet for two weeks before the performance of the experiment. At the beginning, rats divided into eight main group were fed on diets for 45 days as follows: Negative control group (first group) was fed on basal diet. Forty nine rats were fed on basal diet and induced by CCl4, in paraffin oil (50% v/v, 2 ml/Kg) twice weeks subcutaneous injection to induce chronic damage in the liver, then divided into 7 groups numbered from group 2 to group 8. Positive control group rats fed on basal diet till final experiment (second group). Group 3 and 4 rats treated with 50 and 100 ppm ethanolic leaves extracts, respectively. Also, group 5 and 6 treated with 50 and 100 ppm aqueous leaves extracts, respectively. All extracts were fed on orally every day. While, rats in group 7 treated with 1% and 2% dried lion’s foot leaves. At the end of the experimental period, serums were collected to determine liver and renal functions. The liver was removed surgically for histopathological observation. The results revealed that CCl4 intoxication impaired liver function. Serum AST, ALT, ALP and total bilirubin levels were elevated by CCl4 administration, while significant decreasing was noticed in serum albumin in CCl4 group. Histopathologically, CCl4 caused congestion of central vain, fatty change of hepatocytes, and focal inflammatory cells in filtration. Treatment with lion’s foot with different forms and concentration attenuated these adverse effects and markedly ameliorated histopathological and biochemical alterations caused by CCl4 especially with 2% powder and 100 ppm ethanol extract administration. Therefore, the results of this study concluded that lion’s foot can be proposed to protect hepatotoxicity induced by CCl4 in rats. The results also revealed that the hepatoprotection effect of lion’s foot may be attributed to its antioxidant contents and free radical scavenger effect.
文摘目的探究高压氧联合硫辛酸治疗糖尿病足的临床疗效。方法选取2020年5月至2022年5月河南省人民医院豫东分院收治的92例糖尿病足患者作为研究对象,按照不同治疗方法将其分为观察组(32例)、对照组(30例)和空白组(30例)。观察组患者在常规治疗的基础上行高压氧联合硫辛酸治疗,对照组患者在常规治疗的基础上单纯行硫辛酸治疗,空白组患者单纯行常规治疗,对比观察3组患者临床疗效、血糖相关指标及血管功能相关因子水平与踝肱指数。结果治疗4周后,3组患者临床疗效对比,观察组明显优于空白组(Z=-2.429,P=0.015),而观察组与对照组以及对照组与空白组间无明显差异(Z=-1.415、-1.299,P=0.157、0.194)。治疗4周后,3组患者空腹血糖(FBG)、餐后2 h血糖(2 h PBG)、糖化血红蛋白(HbAlc)水平对比,观察组均明显低于空白组(q=6.574、6.162、6.339,P均<0.001),而观察组与对照组以及对照组与空白组间无明显差异(FBG:q=3.310、3.213,P=0.055、0.065;2 h PBG:q=2.840、3.270,P=0.116、0.059;HbAlc:q=3.230、3.059,P=0.063、0.083);3组患者血清内皮素(ET)水平对比,观察组明显低于对照组与空白组(q=3.443、6.630,P=0.044、P<0.001),而对照组与空白组间无明显差异(q=3.137,P=0.074);3组患者血清降钙素基因相关肽(CGRP)、血管内皮生长因子(VEGF)水平对比,观察组均明显高于对照组与空白组(CGRP:q=3.491、6.883,P=0.041、P<0.001;VEGF:q=3.467、6.636,P=0.042、P<0.001),而对照组与空白组间无明显差异(q=3.339、2.851,P=0.053、0.114)。治疗4周后,3组患者踝肱指数对比,观察组明显高于对照组与空白组(q=3.947、7.330,P=0.018、P<0.001),而对照组与空白组间无明显差异(q=3.330,P=0.054)。结论高压氧联合硫辛酸治疗糖尿病足,有助于控制血糖水平、改善血管内皮功能及下肢血管病变程度,提高治疗效果,临床推广价值较高。
基金supported by a grant from the Shandong Science and Technology Development Plan Project in China,No.2012YD18031
文摘No recommended guidelines currently exist for the therapeutic concentration or dose of botulinum toxin type A （BTXA） injected into the muscle to treat limb spasticity. Therefore, in this randomized controlled trial, we explored the safety and efficacy of two concentrations and two doses of BTXA in the treatment of spastic toot after stroke to optimize this treatment in these patients. Eligible patients （n = 104） were randomized into four groups. The triceps surae and tibialis posterior on the affected side were injected with BTXA at one of two doses （200 U or 400 U） and two concentrations （50 U/mL or 100 U/mL）. 2he following assessments were conducted before as well as 4 days and 1, 2, 4, and 12 weeks after treatment： spasticity, assessed using the modified Ashworth scale; basic functional mobility, assessed using a timed up and go test; pace, assessed using a 10-meter timed walking test; and the ability to walk, assessed using Holden＇s graded scale and a visual analog scale. The reported results are based on the 89 patients that completed the study. We found significant differences for the two doses and concentrations of BTXA to improve the ability of patients to walk independently, with the high-dose/low-concentration combination providing the best effect. Onset and duration of the ameliorating effects of BTXA were 4-7 days and 12 weeks, respectively. Thus, BTXA effectively treated foot spasms after stroke at an optimal dose of 400 U and concentration of 50 U/mL.
