EV71-type virus is one of the main pathogens causing the occurrence of hand,foot and mouth disease(HFMD),and VP1 protein,a factor that directly determines the antigenicity of the virus,has been isolated.The tomato was...EV71-type virus is one of the main pathogens causing the occurrence of hand,foot and mouth disease(HFMD),and VP1 protein,a factor that directly determines the antigenicity of the virus,has been isolated.The tomato was selected as a bioreactor for the production of an edible EV71 vaccine designed for the VP1 capsid protein.Using molecular biology techniques,the fusion gene EV71-VP1 was cut from vector PGEX-4T-2,a vector containing the p2300-EV71 gene with CaMV35S promoter and TL regulatory elements was constructed,and the hypocotyl and cotyledons of tomato were transformed using Agrobacterium(EHA105)-mediated method,screened,elongated and rooted,and finally 20 resistant tomato plants were obtained.Five transgenic positive seedlings were obtained by digestion and PCR assay,among which three plants were detected by RT-PCR to be capable of transcriptional translation at the RNA level.The experimental results aimed to explore new material support for the preparation of transgenic plant oral vaccines against EV71 infection and provide a theoretical basis for accelerating the development of transgenic plant vaccines in the future.展开更多
对动物免疫成功与否,要看能否将易感群体减少到传染病可流行的阈值以下,因此免疫接种的广泛性是控制人畜疫病流行的重要因素之一。尽管医药科学已有飞速发展,但是由于疫苗的生产、配送等方面的限制,全球推广免疫接种仍然面临着巨大的困...对动物免疫成功与否,要看能否将易感群体减少到传染病可流行的阈值以下,因此免疫接种的广泛性是控制人畜疫病流行的重要因素之一。尽管医药科学已有飞速发展,但是由于疫苗的生产、配送等方面的限制,全球推广免疫接种仍然面临着巨大的困难。开发使用方便、廉价、高效疫苗,对控制全球各种疾病的爆发和流行有着深远的意义。目前,广泛研究的可食疫苗(Ed ib le Vacc ine)为解决这一难题提供了新的思路和方法。可食疫苗不仅有使用方便、安全的优点,而且一旦在转基因植物、动物或微生物中研究推广,可以立即为落后贫困地区人畜疫病防疫提供廉价可行的可食疫苗。本文主要综述目前广泛研究的几种可食疫苗的研究进展,主要包括:转基因植物可食疫苗、沙门氏菌携带的重组和DNA核酸可食疫苗、其他微生物携带或重组的可食疫苗,以及转基因动物生产的可食疫苗。展开更多
Elevated expression of the rotavirus VP6 antigen in transgenic plants is a critical factor in the development of a safe and effective rotavirus vaccine. Using codon optimization, a gene that encodes the inner capsid p...Elevated expression of the rotavirus VP6 antigen in transgenic plants is a critical factor in the development of a safe and effective rotavirus vaccine. Using codon optimization, a gene that encodes the inner capsid protein VP6 of the human group A rotavirus was synthesized （sVP6）. The VP6 and sVp6 genes were transformed into tobacco （Nicotiana tabacum L.） plants using Agrobacterium tumefaciens. The expression level of the sVP6 gene in transgenic plants was 3.8-34-fold higher than that of controls containing the non-modified VP6 gene, accounting for up to 0.34% of the total soluble protein （TSP）. Then, BALB/ c female mice that had been gavaged weekly with 10 mg TSP containing 34 p.g VP6 protein, in which VP6-specific serum IgG and mucosal IgA antibodies were investigated. The severity and duration of diarrhea caused by simian rotavirus SA-11 challenge were reduced significantly in passively immunized pups, which indicates that anti-VP6 antibodies generated in orally immunized female mice can be passed onto pups and provide heterotypic protection. An edible vaccine based on the VP6 of human rotavirus group A could provide a means to protect children and young animals from severe acute diarrhea.展开更多
基金Supported by the Natural Science Foundation of Heilongjiang Province(LH2021C032)。
文摘EV71-type virus is one of the main pathogens causing the occurrence of hand,foot and mouth disease(HFMD),and VP1 protein,a factor that directly determines the antigenicity of the virus,has been isolated.The tomato was selected as a bioreactor for the production of an edible EV71 vaccine designed for the VP1 capsid protein.Using molecular biology techniques,the fusion gene EV71-VP1 was cut from vector PGEX-4T-2,a vector containing the p2300-EV71 gene with CaMV35S promoter and TL regulatory elements was constructed,and the hypocotyl and cotyledons of tomato were transformed using Agrobacterium(EHA105)-mediated method,screened,elongated and rooted,and finally 20 resistant tomato plants were obtained.Five transgenic positive seedlings were obtained by digestion and PCR assay,among which three plants were detected by RT-PCR to be capable of transcriptional translation at the RNA level.The experimental results aimed to explore new material support for the preparation of transgenic plant oral vaccines against EV71 infection and provide a theoretical basis for accelerating the development of transgenic plant vaccines in the future.
文摘对动物免疫成功与否,要看能否将易感群体减少到传染病可流行的阈值以下,因此免疫接种的广泛性是控制人畜疫病流行的重要因素之一。尽管医药科学已有飞速发展,但是由于疫苗的生产、配送等方面的限制,全球推广免疫接种仍然面临着巨大的困难。开发使用方便、廉价、高效疫苗,对控制全球各种疾病的爆发和流行有着深远的意义。目前,广泛研究的可食疫苗(Ed ib le Vacc ine)为解决这一难题提供了新的思路和方法。可食疫苗不仅有使用方便、安全的优点,而且一旦在转基因植物、动物或微生物中研究推广,可以立即为落后贫困地区人畜疫病防疫提供廉价可行的可食疫苗。本文主要综述目前广泛研究的几种可食疫苗的研究进展,主要包括:转基因植物可食疫苗、沙门氏菌携带的重组和DNA核酸可食疫苗、其他微生物携带或重组的可食疫苗,以及转基因动物生产的可食疫苗。
文摘Elevated expression of the rotavirus VP6 antigen in transgenic plants is a critical factor in the development of a safe and effective rotavirus vaccine. Using codon optimization, a gene that encodes the inner capsid protein VP6 of the human group A rotavirus was synthesized （sVP6）. The VP6 and sVp6 genes were transformed into tobacco （Nicotiana tabacum L.） plants using Agrobacterium tumefaciens. The expression level of the sVP6 gene in transgenic plants was 3.8-34-fold higher than that of controls containing the non-modified VP6 gene, accounting for up to 0.34% of the total soluble protein （TSP）. Then, BALB/ c female mice that had been gavaged weekly with 10 mg TSP containing 34 p.g VP6 protein, in which VP6-specific serum IgG and mucosal IgA antibodies were investigated. The severity and duration of diarrhea caused by simian rotavirus SA-11 challenge were reduced significantly in passively immunized pups, which indicates that anti-VP6 antibodies generated in orally immunized female mice can be passed onto pups and provide heterotypic protection. An edible vaccine based on the VP6 of human rotavirus group A could provide a means to protect children and young animals from severe acute diarrhea.
