Former Botanical Garden of ORSTOM(Côte d’Ivoire):What Remain after Thirty Years of Abandonment and Forest Restoration

Botanical gardens represent important places for ex situ conservation.One of these botanical gardens has been abandoned in Côte d’Ivoire for 30 years.This is the former botanical garden of ORSTOM.This study was conducted to determine the level of diversity of this former garden in order to assess the opportunity for its rehabilitation.The authors carried out inventories in 18 quadrats of 500 m2 through the vegetation to collect woody species.Dendrometric measurements(height,diameter)were also recorded to assess the structure of the site.A total of 190 species have been identified.They belong to 141 genera and 47 families.This former botanical garden contains important species because of their origin,status or particularity(threatened,endemic,rarity,etc.).A total of 19 threatened species including 2 endangered and west African endemic(Chrysophyllum azaguieanum J.Miège,Placodiscus pseudostipularis)were found at the site.Also,Chrysophyllum azaguieanum is declared extinct from Côte d’Ivoire.Four species are rare in the flora of Côte d’Ivoire:Balanites wilsoniana,Chrysophyllum azaguieanum,Gilletiodendron kisantuense and Loesenera kalantha.The most abundant species is Hopea odorata.Although this introduced species is considered globally vulnerable,it presents a risk of invasion in the forest of Côte d’Ivoire.The diameter and height structures show that all the stages of development are presented indicating a good regeneration on the site.Ultimately,this botanical garden deserves to be rehabilitated and especially urgent management of Hopea odorata is needed to prevent an invasion of this species.

Soil organic carbon storage in forest restoration models and environmental conditions

The scale of forest and landscape restoration is expected to increase during the UN Decade on Ecosystem Restoration and its contribution to the provision of critical ecosystem services to society.Several models of forest restoration may improve ecosystem services,including soil organic carbon(SOC)storage.A review was carried out to access:(1)the variability of SOC storage between worldwide forest restoration models and,(2)the effects of climate,soil class,soil texture,and vegetation type on SOC storage.We reviewed 119 primary studies with information on SOC and soil texture for different forest restoration models.The restoration models were grouped into four categories:natural regeneration,monocultures,agroforestry,and mixed forest.SOC data was extracted from these four restoration models,other land uses(cropland,bare land,grassland,and natural forest),climate regimes,and soil properties.The SOC storage in the forest restoration models and other land uses at a global scale ranged between 0.1 to 514 Mg ha^(-1).The overall mean value for SOC storage was higher for natural regeneration(112 Mg ha^(-1)),followed by agroforestry(74 Mg ha^(-1)),mixed forest(73 Mg ha^(-1)),and monocultures(68 Mg ha^(-1)).However,the average SOC storage was similar among the four restoration models in the moist warm climate zone.The SOC storage mean value in the moist cool zone was 23%higher than the dry cool zone(81 and 62 Mg ha^(-1),respectively),and 50%higher for the moist warm zone when compared to the dry warm climatic zone(74 and 38 Mg ha^(-1),respectively).The SOC storage of the restoration models was positively related to soil depth(0.59;p<0.01),clay content(0.29;p<0.01),and stand age(0.17;p<0.01).Globally,the mean values of SOC storage were 26,66,and 139 Mg ha^(-1) at zero-10,zero-30,and zero-100 cm depths,respectively.In addition,sandy soils showed smaller mean values of SOC storage than medium to clay soils,especially in deeper soil layers.Furthermore,SOC storage was positively related between restoration models and other land uses(0.93;p<0.01),suggesting a prominent effect of climate and soil properties on SOC.Forest restoration models showed larger SOC storage when compared to croplands and bare land,but in general it was smaller or similar when compared to pasture and natural forest.

Integrating species and successional classes for wood production in a mixed forest restoration system in a neotropical region

As forests in neotropical regions,particularly in developing countries,are devastated,interventions to restore biodiversity and its ecological functions are needed.Rural producers have thus been encouraged to grow trees for wood production as an economic activity.The objective of this study was to quantify the increment of wood density of four forest species from different successional classes of a mixed system of restoration in a neotropical forest in Brazil.Tree discs were sampled at breast height and analyzed radially by X-ray densitometry to obtain apparent density and basic density.Three trees each of a species from the pioneer,early and late secondary successional stages and of the dominant species in the climax community.The radial profiles indicated increasing density from the pith to bark of trunks,except for some variations due to wood defects and growth.Average density was 0.576 g cm^(-3),0.655 g cm^(-3),0.706 g cm^(-3)and 0.775 g cm^(-3),respectively,for Peltophorum dubium,Schinus terebinthifolius,Cariniana estrellensis and Hymenaea courbaril.Radial profiles indicated higher amplitudes in the apparent densities for slow-growing species.X-ray densitometry generated parameters such as minimum,average and maximum densities,and radial density variations.These parameters are important for understanding the ecological functional role of successional classes of the Atlantic Forest from the Neotropical region.

Estimating Urban Households’ Willingness-to-Pay for Upland Forest Restoration in Vietnam

Increased urbanization coupled with increased reliance of urban communities on rural areas for ecosystem service provision is a challenge faced by many nations. The ability of urban households to directly support restoration efforts in surrounding rural regions represents an underappreciated funding stream for ecological restoration. This study explored the willingness of urban households to support forest restoration in Vietnam. We surveyed 211 households (HHs) in the capital city Hanoi, Vietnam. A Maximum Likelihood Estimator (MLE) model allowed us to obtain the parameters of our model and quantify mean Willingness-to-Pay (WTP) for a program of forest restoration in addition to identifying factors influencing the decision of WTP. Generally, over forty percent of the households surveyed are willing to pay for forest restoration and the mean value of WTP is 37,830 VND ($1.73) per household per month. WTP depends on endogenous and exogenous factors including level of education, income, female-to-male ratio in the household, attitude toward payment for monthly electricity consumption, and awareness of payment for environmental service. Our results suggest that urban household’s demand for forest restoration is real, and represents an untapped source of restoration funding. Policy-makers should take actions to apply charges on water bills to turn this potential into reality for restoration projects in Vietnam if the benefits from restoration outweigh the costs based on our findings.

Effects of Forest Restoration Techniques on Community Diversity and Aboveground Biomass on Area Affected by Mining Tailings in Mariana,Southeastern Brazil

Currently there is an urgent and special attention in actions to restore tropical forests.In this study,we evaluated the effect of different restoration methods on aboveground biomass(AGB)stock,tree community diversity and structure,in areas affected by the Fundão tailings dam collapse in Mariana,Minas Gerais state,Brazil.We measured and compiled data of the AGB,community diversity and structure attributes in 36 plots distributed in six restoration treatments and six replicas:planting of native tree seedlings with fertilization and pH correction(PSf)and without fertilization and pH correction(PS);seeding of native trees with fertilization and pH correction(SDf)and without fertilization and pH correction(SD);natural regeneration with fertilization and pH correction(NRf)and without fertilization and pH correction(NR).No significant differences in substrate properties and AGB between treatments.Although biomass storage between treatments was not statistically different,there is a clear pattern showing higher values active restoration method.The Pielou index ranged from 0.520(SDf)to 0.943(NR),except for SDf all the others treatments had values higher than 0.76.This result suggests floristic heterogeneity,without ecological dominance in the plant community.Overall,active restoration had important implications for the forest restoration where natural regeneration is limited.

Aspects of forest restoration and hydrology:linking passive restoration and soil–water recovery in Brazilian Cerrado

Although the scientific knowledge about some associations between physical parameters of the soil is consolidated,studies focused on investigating the dynamics of forest restoration processes and the structure of soils remain incipient.The study area is located in Cerrado and is called as Private Natural Heritage Reserve(RPPN)Águas Perenes Forest(perennial water forest)and was selected as a“High Conservation Value Forest”,provides scope to investigate the soil physical properties recovery on passive restoration sites,with different ages but with the same land use history.So,the aim was to investigate the soil density,moisture,penetration resistance and infiltration rate with long-term from old(FA,46 year-old),medium(FB,11 year-old)and young(FC,8 year-old)passive restoration sites.It was observed differences in soil density,as well as in soil moisture,resistance to penetration and infiltration rate.Mean densities recorded for FA,FB and FC were 1.38,1.48 and 1.53 g cm^(-3),respectively.FA recorded higher mean soil moisture(14.9%)than FB(11.5%)and FC(10.0%),whereas mean soil resistance to penetration was increased from FA(0.87 MPa)to FB(0.91 MPa)and FC(1.10 MPa).The average infiltration rate was almost similar in FA and FB(FA=76.43 mm h^(-1);FB=77.48 mm h^(-1))and approximately 39%higher than FC(55.79 mm h^(-1)).Thus,although soil density and moisture were different in each passive restoration site,11 year-old passive restoration site showed similar resistance to penetration and infiltration rate to the old(46 years)passive restoration site.These features allowed seeing improvements in hydrological maintenance in the soil and revealed that passive restoration in Cerrado is able to improve the physical features on a soil presenting similar texture,climate and management history.

Linkage of microbial living communities and residues to soil organic carbon accumulation along a forest restoration gradient in southern China

Background:Forest restoration has been considered an effective method to increase soil organic carbon(SOC),whereas it remains unclear whether long-term forest restoration will continuously increase SOC.Such large uncertainties may be mainly due to the limited knowledge on how soil microorganisms will contribute to SOC accumulation over time.Methods:We simultaneously documented SOC,total phospholipid fatty acids(PLFAs),and amino sugars(AS)content across a forest restoration gradient with average stand ages of 14,49,70,and>90 years in southern China.Results:The SOC and AS continuously increased with stand age.The ratio of fungal PLFAs to bacterial PLFAs showed no change with stand age,while the ratio of fungal AS to bacterial AS significantly increased.The total microbial residue-carbon(AS-C)accounted for 0.95-1.66% in SOC across all forest restoration stages,with significantly higher in fungal residue-C(0.68-1.19%)than bacterial residue-C(0.05-0.11%).Furthermore,the contribution of total AS-C to SOC was positively correlated with clay content at 0-10 cm soil layer but negatively related to clay content at 10-20 cm soil layer.Conclusions:These findings highlight the significant contribution of AS-C to SOC accumulation along forest restoration stages,with divergent contributions from fungal residues and bacterial residues.Soil clay content with stand age significantly affects the divergent contributions of AS-C to SOC at two different soil layers.

A decision framework for identifying models to estimate forest ecosystem services gains from restoration

Restoring degraded forests and agricultural lands has become a global conservation priority. A growing number of tools can quantify ecosystem service tradeoffs associated with forest restoration. This evolving ＂tools landscape＂ presents a dilemma： more tools are available, but selecting appropriate tools has become more challenging. We present a Restoration Ecosystem Service Tool Selector （RESTS） framework that describes key characteristics of 13 ecosystem service assessment tools. Analysts enter information about their decision context, services to be analyzed, and desired outputs. Tools are filtered and presented based on five evaluative criteria： scalability, cost, time requirements, handling of uncertainty, and applicability to benefit-cost analysis. RESTS uses a spreadsheet interface but a web-based interface is planned. Given the rapid evolution of ecosystem services science, RESTS provides an adaptable framework to guide forest restoration decision makers toward tools that can help quantify ecosystem services in support of restoration.

A review of the roles of forest canopy gaps

Treefall gap, canopy opening caused by the death of one or more trees, is the dominant form of disturbance in many forest systems worldwide. Gaps play an important role in forest ecology helping to pre- serve bio- and pedo-diversity, influencing nutrient cycles, and maintain- ing the complex structure of the late-successional forests. Over the last 30 years, numerous reviews have been written describing gap dynamics. Here we synthesize current understanding on gap dynamics relating to tree regeneration with particular emphasis on gap characteristics consid- ered critical to develop ecologically sustainable forest management sys- tems and to conserve native biodiversity. Specifically, we addressed the question： how do gaps influence forest structure？ From the literature re- viewed, the size of gaps induces important changes in factors such as light intensity, soil humidity and soil biological properties that influence tree species regeneration and differ in gaps of different sizes. Shade- tolerant species can colonize small gaps; shade-intolerant species need large gaps for successful regeneration. Additionally, gap dynamics differ between temperate, boreal, and tropical forests, showing the importance of climate differences in driving forest regeneration. This review summa- rizes information of use to forest managers who design cutting regimes that mimic natural disturbances and who must consider forest structure, forest climate, and the role of natural disturbance in their designs.

Changes in leaf stomatal traits of different aged temperate forest stands

Stomata control carbon and water vapor exchange between the leaves and the atmosphere,thus infl uencing photosynthesis and transpiration.Combinations of forest patches with different stand ages are common in nature,however,information of which stomatal traits vary among these stands and how,remains limited.Here,seven different aged forest stands(6,14,25,36,45,55,and 100 years)were selected in typical temperate,mixed broadleaf-conifer forests of northeast China.Stomatal density,size and relative area of 624 species,including the same species in stands of different ages were selected.Stomatal density,size and relative area were distributed log-normally,differing across all species and plant functional groups.Stomatal density ranged from 4.2 to 1276.7 stomata mm^(–2),stomatal size ranged from 66.6 to 8315.7μm^(2),and stomatal relative area 0.1–93.3%.There was a significant negative relationship between density and size at the species and functional group levels,while the relative stomatal area was positively correlated with density and size.Stomatal traits of dominant species were relatively stable across different stand ages but were significantly different for herbs.The results suggest that stomatal traits remain relatively stable for dominant species in natural forests and therefore,spatial variation in stomatal traits across forest patches does not need to be incorporated in future ecological models.

A road for a promising future for China＇s primates： The potential for restoration

China is one of the most dynamic countries of the world and it shelters some amazing levels of biodiversity, including some very special primate species. However, primarily as a result of forest loss, most of which occurred in historical times, approximately 70% of China＇s primate species have less than 3 000 individuals. Here I evaluate one road for future conservation/development that could produce very positive gains for China＇s primates; namely forest restoration. I argue that for a large scale restoration project to be possible two conditions must be met; the right societal conditions must exist and the right knowledge must be in hand. This evaluation suggests that the restoration of native forest to support many of China＇s primates holds great potential to advance conservation goals and to promote primate population recovery.

Effects of silviculture treatments in a hurricane-damaged forest on carbon storage and emissions in central Hokkaido, Japan

Hurricanes cause abrupt carbon reduction in forests, but silviculture treatment can be an effective means of quickly regenerating and restoring hurricane-damaged sites. This study assessed how silviculture treatments affect carbon balance after hurricane damage in central Hokkaido, Japan. We examined carbon storage in trees and underground vegetation as well as carbon emissions from silviculture operations in 25-year-old stands, where scarification and plantation occurred just after hurricane damage. The amount of carbon stored varied according to silviculture treatment. Among three scarification treatments, a scarified depth of 0 cm （understory vegetation removal） led to the largest amount of carbon stored （64.7 t·ha^-1 C）. Among four plantation treatments, the largest amount of carbon was stored in a Larix hybrid （L. gmelinii var. japonica × L. kaempferi） plantation （80.3 t·ha^-1 C）. The plantation of Abies sachalinensis was not successful at accumulating carbon （40.5·ha^-1 C）. The amount of carbon emitted from silviculture operations was 0.05-0.14 t·ha^-1 C, and it marginally affected the net carbon balance of the silviculture project. Results indicate that silviculture treatments should beperformed in an appropriate way to effectively recover the ability of carbon sequestration in hurricane-damaged forests.

Neighborhood diversity structure and neighborhood species richness effects differ across life stages in a subtropical natural secondary forest

Natural secondary forest has a strong capacity to regrow naturally and recover biodiversity rapidly on abandoned lands.However,at the neighborhood scale,which can truly reflect the facilitative or competitive interactions among individual plants,the local diversity spatial structure in secondary forest and the feedback effects of neighborhood diversity on natural regeneration remain unclear,and this may be the key to properly understand the mechanisms of natural secondary forest species diversity recovery.To this end,this study established a dynamic plot in a rehabilitated secondary forest after disturbance and conducted a comprehensive survey of 68,336 individual plants with repeated measurements at 5-year interval to assess the characteristics of neighborhood diversity structure across life history stages and link the neighborhood species richness(NSR)effect translated by species interactions at species diversity structure with individual trees recruitment/mortality in secondary forest regeneration.The results showed that,compared with tropical and temperate natural forests,a higher proportion of diversity accumulators and a lower proportion of repellers in subtropical secondary forests resulted in neighborhood diversity structures characterized by heterospecific or high-diversity patches,which are beneficial to the maintenance or restoration of biodiversity.As an important supplement to the research on the relationship between diversity and productivity,our findings show a positive diversity-survival relationship in subtropical secondary forests.Importantly,we observed that the neighborhood diversity structure exhibited a trend of accumulator-dominated to neutral-dominant changes with life stage from sapling to adult,which,in turn,determined the direction and strength of NSR effects on recruitment/mortality.Specifically,diverse local neighborhoods at a later successional stage characterized with‘neutral’species-species interactions can act as a‘welfare net’by offering favorable microhabitats for the most vulnerable recruitments or saplings,i.e.,the NSR effects that promoted individual recruitment/survival in our study.These results not only enrich our understanding of the biodiversity-productivity-survival relationship but also highlight the importance of retaining latesuccessional species of native trees in intensive forest production or in situ conservation policies.

Conceptual models of forest dynamics in environmental education and management:keep it as simple as possible,but no simpler

Background：Conceptual models of forest dynamics are powerful cognitive tools,which are indispensable for communicating ecological ideas and knowledge,and in developing strategic approaches and setting targets for forest conservation,restoration and sustainable management.Forest development through time is conventionally described as a directional,or ＂linear＂,and predictable sequence of stages from ＂bare ground＂ to old forest representing the ＂climax-state＂.However,this simple view is incompatible with the current knowledge and understanding of intrinsic variability of forest dynamics.Hypothesis：Overly simple conceptual models of forest dynamics easily become transformed into biased mental models of how forests naturally develop and what kind of structures they display.To be able to communicate the essential features and diversity of forest dynamics,comprehensive conceptual models are needed.For this end,Kuuluvainen（2009） suggested a relatively simple conceptual model of forest dynamics,which separates three major modes of forest dynamics,and incorporates state changes and transitions between the forest dynamics modes depending on changes in disturbance regime.Conclusions：Conceptual models of forest dynamics should be comprehensive enough to incorporate both longterm directional change and short-term cyclic forest dynamics,as well as transitions from one dynamics mode to another depending on changes in the driving disturbance regime type.Models that capture such essential features of forest dynamics are indispensable for educational purposes,in setting reference conditions and in developing methods in forest conservation,restoration and ecosystem management.

Analysis of Land Use and Land Cover Changes and Evaluation of Natural Generation and Potential Restoration Areas in the Mexican Huasteca Region

This study aims to analyze temporal changes in land use and land cover change (LUCC) as well as identify areas for natural regeneration and potential areas for forest restoration in the Huasteca region for the period from 1976 to 2007. Changes were quantified in numbers and, additionally, cartography was used to identify and map the main affected areas. Different models based on Geographic Information Systems (GIS) demonstrated that LUCC have occurred on an area of 11718.82 km2, representing 17.84% of the region’s surface. Agriculture and the growth of pasture could be identified as the main human-induced activities that have led to landscape modification. In addition, forest cover is affected by a deforestation rate which is higher than the national average. Further important changes include a change from natural land cover to non-original land cover, affecting an area of 4911.88 km2 in the period from 1976-1993, and an area of 1892.5 km2 in the period from 1993-2002. Smaller changes could be observed for the period from 1993-2002 with an affected area of 1029.78 km2. At the same time, a natural regeneration from nonoriginal to original land cover took place from 1976-1993 on an area of 1318.68 km2, and also on an area of 974.18 km2 between 1993 an 2002. The surface that underwent a natural regeneration of forest cover made up 1932.07 km2. At the same time, an area of 5739.29 km2 for potential forest restoration was identified. Drawing on GIS methods and techniques, the development of thematic maps for land use, land use and land cover changes for the years of analysis (1976-1993-2002-2007) proved to be very adequate for the evaluation and analysis of the land cover and land use change, in particular with regard to the decrease of natural vegetation cover.

Alleviating forest degradation in the Lancang-Mekong Region requires closing management—measurement gaps

Deforestation and forest degradation are having profound negative impacts on social-ecological systems in the emerging economies across the tropics.Consequently,interest in restoring and rehabilitating degraded forests has been growing.This paper explores current issues related to addressing forest degradation in the Lancang-Mekong Region(LMR)of Southeast Asia through a review of the use of criteria and indicators for forest degradation and rehabilitation.Forest degradation must be understood in the context of its underlying drivers,which are numerous and complex.Understanding these underlying drivers of degradation requires diagnosing the entwined political,social,economic,and environmental systems that aff ect forests.Landscapes are the relevant scales to diagnose and intervene for improved forests.Interventions to restore or rehabilitate forests should be process-driven,focused on the underlying social,ecological and political processes that degrade landscapes.Interventions should also include negotiation among all actors infl uencing and competing for natural resource claims in forest landscapes.Criteria and indicators for forest landscape restoration should therefore help to improve the governance of forest landscapes.Criteria and indicators provide measures of the biophysical outcomes of degradation,in addition to processes,but these should be adapted to changing contexts and emerging challenges,and should rectify any pre-existing fl awed change-logic.Restoration activities should synthesize,integrate,and build upon the rich history of pre-existing restoration guidelines,but should be adaptable in order to be applied eff ectively in the contexts of local landscapes.This project is facilitating a dialogue around the use of criteria and indicators to help solve the degradation challenge in the LMR.We will trial the use of the criteria and indicators generated through this research in the LMR to learn what works and what doesn’t.This will provide an opportunity to build consensus around the ways in which restoration investments made by governments,civil society,and the private sector can infl uence sustainability.

Effects of submergence in water on seed germination and vigor of the Copaifera lucens(Fabaceae) seedlings

We analyzed the tolerance of Copaifera lucens seeds to sub- mersion in water to assess the use of this species for direct seeding in riparian forest restoration programs. Seeds were submerged in water for 2, 4, 8, 16 and 32 days or not submerged （control = 0 days of submer- gence）. For the control and at the end of each period of submersion, germination and seedling vigor tests were carried out. For germination tests, seeds were sown in plastic pots containing sand and kept in labora- tory conditions. The percentage of seed germination, the germination rate and the average germination time were analyzed. For seedlings, total biomass, leaf area, leaf mass per area and leaf area ratio were analyzed. Submersion time drastically affected the dissolved oxygen content and seed germination. Between 4 and 8 days of submersion there was a de- crease from 83.8% to 15.6% in the germination percentage. No seed germination occurred after 16 days of submersion. Although there was a significant decrease in the percentage of seed germination between 4 and 8 days of submersion, seedling vigor was not affected. Seeds of this species were partially tolerant to submersion in water, suggesting that C. lucens is a promising species for direct seeding in riparian forest restora- tion projects.

Shade shelters increase survival and photosynthetic performance of oak transplants at abandoned fields in semi-arid climates

Forest restorations conducted in semiarid, seasonally dry climates must deal with the intense drought stress that affects tree seedlings during the dry season. Although this water deficit is the most commonly invoked source of mortality for seedlings, several other environmental factors may also preclude survival of transplants. For instance, it has been widely reported that excessive light reduces the efficiency of the photosynthetic apparatus, hence decreasing plant survival, but most seedling transplants in deforested areas are conducted under these light conditions. This study is focused in determining whether excessive light affects the photosynthetic performance and survival of Quercus coccolobifolia, a Mexican oak species, when their seedlings are transplanted in semiarid deforested areas. Further, this study tests the possibility of using artificial shade shelters to improve the ecophysiological performance and survival of seedlings. Oak seedlings were transplanted under full sunlight conditions and beneath artificial shade shelters of two different colors： white and black. To reduce water stress, and hence isolate the effects of light treatments, a drip irrigation system was implemented at each experimental plot. Seedling survival was monitored weekly for 128 days and photosynthetic performance was assessed by measuring chlorophyll fluorescence at three opportunities during the experiment. Sun-exposed seedlings showed lower photosynthetic performance and survival rates than those beneath shelters of both colors. These results suggest that sunlight damage can reduce seedling survival when they are transplanted in exposed sites, and that shade shelters can improve the success of forest restoration programs in semiarid climates.

Impact of Robinia pseudoacacia stand conversion on soil properties and bacterial community composition in Mount Tai,China

Background:Robinia pseudoacacia is a widely planted pioneer tree species in reforestations on barren mountains in northern China.Because of its nitrogen-fixing ability,it can play a positive role in soil and forest restoration.After clearcutting of planted stands,R.pseudoacacia stands become coppice plantations.The impacts of shifting from seedling to coppice stands on soil bacterial community and soil properties have not been wel described.This study aims to quantify how soil properties and bacterial community composition vary between planted seedling versus coppice stands.Methods:Nine 20 m×20 m plots were randomly selected in seedling and coppice stands.The bulk soil and rhizosphere soil were sampled in summer 2017.Bulk soil was sampled at 10 cm from the soil surface using a soil auger.Rhizosphere soil samples were col ected using a brush.The soil samples were transported to the laboratory for chemical analysis,and bacterial community composition and diversity was obtained through DNA extraction,16 S r RNA gene amplification and high-throughput sequencing.Results:The results showed that,compared to seedling plantations,soil quality decreased significantly in coppice stands,but without affecting soil exchangeable Mg^(2+) and K^(+).Total carbon(C)and nitrogen(N)were lower in the rhizosphere than in bulk soil,whereas nutrient availability showed an opposite trend.The conversion from seedling to coppice plantations was also related to significant differences in soil bacterial community structure and to the reduction of soil bacterialα-diversity.Principal component analysis(PCA)showed that bacterial community composition was similar in both bulk and rhizosphere soils in second-generation coppice plantations.Special y,the conversion from seedling to coppice stands increased the relative abundance of Proteobacteria and Rhizobium,but reduced that of Actinobacteria,which may result in a decline of soil nutrient availability.Mantel tests revealed that C,N,soil organic matter(SOM),nitrate nitrogen(NO^(-)+(3)-N)and available phosphorus positively correlated with bacterial community composition,while a variation partition analysis(VPA)showed that NO^(-)+(3)-N explained a relatively greater proportion of bacterial distribution(15.12%),compared with C and SOM.Surprisingly,N showed no relationship with bacterial community composition,which may be related to nitrogen transportation.Conclusions:The conversion from seedling to coppice stands reduced soil quality and led to spatial-temporal homogenization of the soil bacterial community structure in both the rhizosphere and bulk soils.Such imbalance in microbial structure can accelerate the decline of R.pseudoacacia.This may affect the role of R.pseudoacacia coppice stands in soil and forest restoration of barren lands in mountain areas.

Land use and land cover change processes in China's eastern Loess Plateau

Using Landsat remote sensing images, we analyzed changes in each land use type and transitions among different land use types during land use and land cover change （LUCC） in Ningwu County, located in the eastern Loess Plateau of China, from 1990 to 2010. We found that grassland, woodland, and farmland were the main land use types in the study area, and the area of each type changed slightly from 1990 to 2010, whereas the area of water, construction land, and unused land increased greatly. For the whole area, the net change and total change were insignificant due to weak human activity intensity in most of the study area, and the LUCC was dominated by quasi-balanced two-way transitions from 1990 to 2010. The insignificant overall amount of LUCC appears to have resulted from offsetting of rapid increases in population, economic growth, and the im- plementation of a program to return farmland to woodland and grassland in 2000. This program converted more farmland into woodland and grassland from 2000 to 2010 than from 1990 to 2000, but reclamation of woodland and grassland for use as farmland continued from 2000 to 2010, and is a cause for concern to the local government.