Using the data of drilling, logging, core, experiments and production, the heterogeneity and differential hydrocarbon accumulation model of deep reservoirs in Cretaceous Qingshuihe Formation(K1q) in the western sectio...Using the data of drilling, logging, core, experiments and production, the heterogeneity and differential hydrocarbon accumulation model of deep reservoirs in Cretaceous Qingshuihe Formation(K1q) in the western section of the foreland thrust belt in southern Junggar Basin are investigated. The target reservoirs are characterized by superimposition of conglomerates, sandy conglomerates and sandstones, with high content of plastic clasts. The reservoir space is mainly composed of intergranular pores. The reservoirs are overall tight, and the sandy conglomerate has the best physical properties. The coupling of short deep burial period with low paleotemperature gradient and formation overpressure led to the relatively weak diagenetic strength of the reservoirs. Specifically, the sandy conglomerates show relatively low carbonate cementation, low compaction rate and high dissolution porosity. The special stress-strain mechanism of the anticline makes the reservoirs at the top of the anticline turning point more reformed by fractures than those at the limbs, and the formation overpressure makes the fractures in open state. Moreover, the sandy conglomerates have the highest oil saturation. Typical anticline reservoirs are developed in deep part of the thrust belt, but characterized by "big trap with small reservoir". Significantly, the sandy conglomerates at the top of anticline turning point have better quality, lower in-situ stress and higher structural position than those at the limbs,with the internal hydrocarbons most enriched, making them high-yield oil/gas layers. The exponential decline of fractures makes hydrocarbon accumulation difficult in the reservoirs at the limbs. Nonetheless, plane hydrocarbon distribution is more extensive at the gentle limb than the steep limb.展开更多
AIMTo determine whether small interfering RNA (siRNA) of PGC-1α could inhibit vascular endothelial growth factor (VEGF) expression and tube formation in human retinal vascular endothelial cells (hRVECs).ME...AIMTo determine whether small interfering RNA (siRNA) of PGC-1α could inhibit vascular endothelial growth factor (VEGF) expression and tube formation in human retinal vascular endothelial cells (hRVECs).METHODShRVECs transfected with peroxisome proliferator-activated receptor-γ coactivator-1α (PGC-1α) siRNA were incubated for 24h and then placed into a normoxic (20%, O<sub>2</sub>) or hypoxic (1%, O<sub>2</sub>) environment for another 16h. PGC-1α mRNA and protein levels were detected by real-time PCR and Western blot. VEGF mRNA and protein levels were detected by real-time PCR and ELISA. Cell proliferation was evaluated by BrdU incorporation assay. Forty-eight hours after siRNA transfection, hRVECs were planted into Matrigel-coated plates and cultured under normoxic (20%, O<sub>2</sub>) or hypoxic (1%, O<sub>2</sub>) conditions for another 48h. The tube formation of hRVECs was observed under an optical microscope and quantified by counting the number of branch points and calculating the total tube length.RESULTSPGC-1α mRNA and protein levels were significantly reduced by PGC-1α siRNA, and VEGF mRNA and protein levels also decreased significantly. The percentage of BrdU-labeled cells in siPGC-1α groups were significantly decreased compared with control siRNA groups under normoxia and hypoxia in cell proliferation assay. In the tube formation assay, PGC-1α siRNA treated cells formed significantly fewer tubes.CONCLUSIONBlocking PGC-1α expression can inhibit VEGF expression in hRVECs and inhibit their ability to form tubes under both normoxic and hypoxic conditions.展开更多
The activation of M1 macrophages can be achieved by stimulating them with lipopolysaccharide （LPS） and interferon-γ （IFN-γ）. However, M1 can be found under physiological conditions without any pathological stimu...The activation of M1 macrophages can be achieved by stimulating them with lipopolysaccharide （LPS） and interferon-γ （IFN-γ）. However, M1 can be found under physiological conditions without any pathological stimuli. This study aimed to understand the involvement of RANKL-induced M1 macrophages in bone formation compared with pathologically induced macrophages. Fischer rats were used to investigate macrophage distribution in normal and injured femoral condyles in vivo. Bone marrow-derived macrophages （BMDMs） were activated with LPS＋IFN-γ and RANKL to achieve M1 activation in vitro. Gene expression related to inflammation, osteoclastogenesis, angiogenesis, and migration was determined by reverse transcription-quantitative polymerase chain reaction （RT-qPCR） and fluorescence-activated cell sorting （FACS）. Tissue macrophages showed distinct expression patterns at different bone regions. RANKL was found in close proximity to inducible nitric oxide synthase-positive （iNOS＋） cells in vivo, suggesting an association between RANKL expression and iNOS＋ cells, especially in trabecular bone. RANKL-induced macrophages showed a different cytokine secretion profile compared with pathologically induced macrophages. Both osteoclasts and M1 macrophages peaked on day 7 during bone healing. RANKL could trigger Ml-like macrophages with properties that were different from those of LPS＋IFN-γ-induced macrophages. These RANKL-activated M1 macrophages were actively involved in bone formation.展开更多
Epoxiconazole is a triazole compound.However,the effects of epoxiconazole on crop productivity and quality were rarely reported.In this study,we investigated the effects of epoxiconazole application on yield formation...Epoxiconazole is a triazole compound.However,the effects of epoxiconazole on crop productivity and quality were rarely reported.In this study,we investigated the effects of epoxiconazole application on yield formation,grain quality attributes,and 2-acetyl-1-pyrroline(2-AP)content in fragrant rice.A three-year field experiment was carried out with a fragrant rice variety,Meixiangzhan 2.At the heading stage,0,0.02,0.04,0.08,0.16 and 0.32 g/L epoxiconazole solutions were foliar applied to fragrant rice plants,respectively.The results showed that epoxiconazole application significantly increased grain yield,seed-setting rate and 1000-grain weight.Chlorophyll content and net photosynthetic rate of fragrant rice during the grain-filling stage significantly increased due to epoxiconazole application.Foliar application of epoxiconazole at 0.08 g/L increased grain protein content and decreased both chalky rice rate and chalkiness area ratio of fragrant rice.Epoxiconazole also substantially increased grain 2-AP content by inducing the regulation in contents of related synthetic precursors,including proline,pyrroline-5-carboxylic acid,Δ1-pyrroline and methylglyoxal.Overall,foliar application of epoxiconazole could be used for the improvement in grain yield,grain quality and 2-AP content in fragrant rice production when applied concentration at 0.08-0.32 g/L.Our findings provided the new roles of epoxiconazole in crop production.展开更多
The Sifangtai and Mingshui formations were continuously cored in the SK-1 n borehole（China Cretaceous Continental Scientific Drilling-SongKe1-the north borehole）.The core is 767.96 m long,and the recovery is 94.7%.T...The Sifangtai and Mingshui formations were continuously cored in the SK-1 n borehole（China Cretaceous Continental Scientific Drilling-SongKe1-the north borehole）.The core is 767.96 m long,and the recovery is 94.7%.The ages of the formations range from middle Campanian to Danian.The sequence and process of lithology-lithofacies and cyclic stratigraphy were described in detail.Eight litho-types compose the Sifangtai Formation,and 15 litho-types compose the Mingshui Formation.Deposition was predominantly in meandering river and lacustrine environments,including 10 microfacies in the Sifangtai Formation and 15 microfacies in the Mingshui Formation.The complete sequence is composed of 535 m-scale cycles（sixth-order cycle）,152 fifth-order cycles,42 fourth-order cycles and five third-order cycles.The centimeter-scale description of the section revealed some previously unknown horizons such as a special type of mudstone,marl,volcanic ash and favorable sand reservoirs in the formations.The new-found evidence is very important for the interpretation of the evolution of the basin,conditions such as lake oxic events,the K/Pg boundary,tectonism in the late sag basin stage,and the reservoir-cap rock assemblages in the shallow stratigraphy.展开更多
Mechanical stimuli play an essential role in maintaining bone remodeling and skeletal integrity.Meanwhile,bone can respond to the changes of mechanical condition to adjust its mass and architecture.Clinical studies di...Mechanical stimuli play an essential role in maintaining bone remodeling and skeletal integrity.Meanwhile,bone can respond to the changes of mechanical condition to adjust its mass and architecture.Clinical studies discover that bedridden patients showed osteoporotic T-scores and low bone mineral density,and long-term immobilized patients presented reduced markers of bone formation.However,as bone formation mediated by osteoblast differentiation is a complex process,the underlying molecular mechanism of mechanical stimuli regulating bone formation is still unclear.Recent evidences show that microRNAs(miRNAs)are involved in mechanical stimuli regulating bone formation or osteoblast differentiation.Nevertheless,no direct evidence identifies mechanoresponsive miRNA in both human and animal bones,and clarifies its mechanoresponsive role under different mechanical conditions(e.g.mechanical unloading,reloading,loading).In the current study,we screened for differentially expressed miRNAs in bone specimens of bedridden patients with fractures,then identified that the expression of miR-138-5p,but not the other miRNAs,altered withbedridden time and was negatively correlated with the expression of the bone formation marker genes Alp(alkaline phosphatase).Moreover,miR-138-5p was up-regulated with reduced bone formation during unloading and down-regulated with increased bone formation during reloading in hind4imb unloaded mice.In addition,miR-138-5p was verified to be responsive to different mechanical unloading condition and cyclic mechanical stretch condition in primary osteogenic cells,respectively.Further in vitro data suggested that mechanoresponsive miR-138-5p directly targeted microtubule actin crosslinking factor 1(MACF1)to inhibit osteoblast differentiation.In vivo,we constructed an osteoblastic miR-138-5p transgenic mice model(TG138)with the Runx2promoter,and found that overexpression miR-138-5p supressed bone formation.Moreover,osteoblast-targeted inhibition of miR-138-5p sensitized bone anabolic response to mechanical loading in TG138 mice.Predominantly,the osteoblast-targeted inhibition of miR-138-5p could counteract bone formation reduction induced by hind limb unloading.Taken together,the mechanoresponsive miR-138-5p inhibited bone anabolic response for developing a novel bone anabolic sensitization strategy.展开更多
The formation of nerve bundles,which is partially regulated by neural cell adhesion molecule 1(NCAM1),is important for neural network organization during peripheral nerve regeneration.However,little is known about how...The formation of nerve bundles,which is partially regulated by neural cell adhesion molecule 1(NCAM1),is important for neural network organization during peripheral nerve regeneration.However,little is known about how the extracellular matrix(ECM)microenvironment affects this process.Here,we seeded dorsal root ganglion tissue blocks on different ECM substrates of peripheral nerve ECM-derived matrixgel,Matrigel,laminin 521,collagen I,and collagen IV,and observed well-aligned axon bundles growing in the peripheral nerve ECM-derived environment.We confirmed that NCAM1 is necessary but not sufficient to trigger this phenomenon.A protein interaction assay identified collagen VI as an extracellular partner of NCAM1 in the regulation of axonal fasciculation.Collagen VI interacted with NCAM1 by directly binding to the FNIII domain,thereby increasing the stability of NCAM1 at the axolemma.Our in vivo experiments on a rat sciatic nerve defect model also demonstrated orderly nerve bundle regeneration with improved projection accuracy and functional recovery after treatment with 10 mg/m L Matrigel and 20μg/m L collagen VI.These findings suggest that the collagen VI-NCAM1 pathway plays a regulatory role in nerve bundle formation.This study was approved by the Animal Ethics Committee of Guangzhou Medical University(approval No.GY2019048)on April 30,2019.展开更多
In order to evaluate the geological characteristics and gas-bearing factors of Niutitang Formation within the Lower Cambrian of northern Guizhou,the Huangping area located at the southern edge of the ancient uplift be...In order to evaluate the geological characteristics and gas-bearing factors of Niutitang Formation within the Lower Cambrian of northern Guizhou,the Huangping area located at the southern edge of the ancient uplift belt of Xuefeng Mountain was selected as the target area,and Well Huangdi 1 was drilled for the geological survey of shale gas.Through geological background analysis and well logging and laboratory analysis such as organic geochemical test,gas content analysis,isothermal adsorption,and specific surface area experiments on Well Huangdi 1,the results show that the Niutitang Formation is a deep-water shelf,trough-like folds and thrust fault.The thickness of black shale is 119.95 m,of which carbonaceous shale is 89.6 m.The average value of organic carbon content is 3.55%,kerogen vitrinite reflectance value is 2.37% and kerogen type is sapropel-type.The brittle mineral content is 51%(quartz 38%),clay mineral content is 38.3%.The value of porosity and permeability are 0.5%and 0.0014 mD,which the reservoir of the Niutitang Formation belongs to low permeability with characteristics of ultra-low porosity.The gas content is 0.09‒1.31 m^3/t with a high-value area and a second high-value area.By comparing with the geological parameters of adjacent wells in the adjacent area,the accumulation model of“sediment control zone,Ro control zone,structure controlling reservoir”in the study area is proposed.Therefore,deep-water shelf-slope facies,Ro is between high maturity-early stage of overmaturity and well-preserved zones in the Niutitang Formation in this area are favorable direction for the next step of shale gas exploration.展开更多
The inhibitory effect of parvovirus H-1 on the colonyforming ability in vitro of QGY-7703, a cultured human hepatoma cell line, and on the formation and growth of its tumors in nude mice was studied. With higher multi...The inhibitory effect of parvovirus H-1 on the colonyforming ability in vitro of QGY-7703, a cultured human hepatoma cell line, and on the formation and growth of its tumors in nude mice was studied. With higher multiplicity of infection (MOI) of H-1 given, survival of the QGY-7703 cells was found to be decreased. H-1 DNA amplification level at 30 h postinfection(p.i.) was detected to be 7.4 times higher than that at 2 h by dispersed cells assay, while the cells were delayed to enter into S phase.Plaques were formed in the indicator cells (new-born human kidney cell line, NBK) by progeny H-1 virus particles released from the infected QGY-7703 cells by infectious cell center assay. The formation of tumors in nude mice by QGY-7703 cells which were injected s c at 2 h postinfection was observed to be prevented in 2 groups with given MOI 25 and 50. The tumor growth of MOI 10 group occurred at a lower exponential rate than that of control,after a 20 d latent period. It was evident that parvovirus H-1 exhibited a direct inhibitory effect on the formation and growth of human hepatoma cells in vivo as well as in vitro.展开更多
This study aimed to investigate the effect and mechanism of valproic acid(VPA)on the neurosphere formation in rat embryonic cortical cells.We used free-floating neurosphere formation as a model system to evaluate the ...This study aimed to investigate the effect and mechanism of valproic acid(VPA)on the neurosphere formation in rat embryonic cortical cells.We used free-floating neurosphere formation as a model system to evaluate the VPA on the proliferation of neural stem cells(NSCs).We found a time-and dose-dependent increase in neurosphere formation and NSC proliferation after VPA treatment.Further RNA-seq analysis demonstrated that the upregulated TGFβ1 signaling might attribute to the effect of VPA on the neurosphere formation and NSC proliferation.Consistently,the neurosphere formation and NSC proliferation were blocked by the treatment with SB431542,an inhibitor of TGFβ1 receptor.Moreover,in a coculture system,NSCs treated with VPA significantly reduced the oxygen-glucose deprivation-induced neuronal apoptosis.Taken together,our results showed that VPA could enhance neurosphere formation and NSC proliferation by activating TGFβ1,which might be a novel therapeutic strategy for neurological disorders.展开更多
基金Supported by the National Natural Science Foundation of China (41902118)Natural Science Foundation of Xinjiang Uygur Autonomous Region (2022D01B141)+1 种基金Natural Science Foundation of Heilongjiang Province (LH2021D003)Heilongjiang Postdoctoral Fund (No.LBH-Z20045)。
文摘Using the data of drilling, logging, core, experiments and production, the heterogeneity and differential hydrocarbon accumulation model of deep reservoirs in Cretaceous Qingshuihe Formation(K1q) in the western section of the foreland thrust belt in southern Junggar Basin are investigated. The target reservoirs are characterized by superimposition of conglomerates, sandy conglomerates and sandstones, with high content of plastic clasts. The reservoir space is mainly composed of intergranular pores. The reservoirs are overall tight, and the sandy conglomerate has the best physical properties. The coupling of short deep burial period with low paleotemperature gradient and formation overpressure led to the relatively weak diagenetic strength of the reservoirs. Specifically, the sandy conglomerates show relatively low carbonate cementation, low compaction rate and high dissolution porosity. The special stress-strain mechanism of the anticline makes the reservoirs at the top of the anticline turning point more reformed by fractures than those at the limbs, and the formation overpressure makes the fractures in open state. Moreover, the sandy conglomerates have the highest oil saturation. Typical anticline reservoirs are developed in deep part of the thrust belt, but characterized by "big trap with small reservoir". Significantly, the sandy conglomerates at the top of anticline turning point have better quality, lower in-situ stress and higher structural position than those at the limbs,with the internal hydrocarbons most enriched, making them high-yield oil/gas layers. The exponential decline of fractures makes hydrocarbon accumulation difficult in the reservoirs at the limbs. Nonetheless, plane hydrocarbon distribution is more extensive at the gentle limb than the steep limb.
基金Supported by National Natural Science Fundation of China(No.81000387)
文摘AIMTo determine whether small interfering RNA (siRNA) of PGC-1α could inhibit vascular endothelial growth factor (VEGF) expression and tube formation in human retinal vascular endothelial cells (hRVECs).METHODShRVECs transfected with peroxisome proliferator-activated receptor-γ coactivator-1α (PGC-1α) siRNA were incubated for 24h and then placed into a normoxic (20%, O<sub>2</sub>) or hypoxic (1%, O<sub>2</sub>) environment for another 16h. PGC-1α mRNA and protein levels were detected by real-time PCR and Western blot. VEGF mRNA and protein levels were detected by real-time PCR and ELISA. Cell proliferation was evaluated by BrdU incorporation assay. Forty-eight hours after siRNA transfection, hRVECs were planted into Matrigel-coated plates and cultured under normoxic (20%, O<sub>2</sub>) or hypoxic (1%, O<sub>2</sub>) conditions for another 48h. The tube formation of hRVECs was observed under an optical microscope and quantified by counting the number of branch points and calculating the total tube length.RESULTSPGC-1α mRNA and protein levels were significantly reduced by PGC-1α siRNA, and VEGF mRNA and protein levels also decreased significantly. The percentage of BrdU-labeled cells in siPGC-1α groups were significantly decreased compared with control siRNA groups under normoxia and hypoxia in cell proliferation assay. In the tube formation assay, PGC-1α siRNA treated cells formed significantly fewer tubes.CONCLUSIONBlocking PGC-1α expression can inhibit VEGF expression in hRVECs and inhibit their ability to form tubes under both normoxic and hypoxic conditions.
基金supported by the CSC (China Scholarship Council)-QUT (Queensland University of Technology) PhD Scholarship awarded to Ms Rong Huangthe Institute of Health and Biomedical Innovation Early Career Researcher Scheme Funding awarded to Dr Yinghong Zhou
文摘The activation of M1 macrophages can be achieved by stimulating them with lipopolysaccharide （LPS） and interferon-γ （IFN-γ）. However, M1 can be found under physiological conditions without any pathological stimuli. This study aimed to understand the involvement of RANKL-induced M1 macrophages in bone formation compared with pathologically induced macrophages. Fischer rats were used to investigate macrophage distribution in normal and injured femoral condyles in vivo. Bone marrow-derived macrophages （BMDMs） were activated with LPS＋IFN-γ and RANKL to achieve M1 activation in vitro. Gene expression related to inflammation, osteoclastogenesis, angiogenesis, and migration was determined by reverse transcription-quantitative polymerase chain reaction （RT-qPCR） and fluorescence-activated cell sorting （FACS）. Tissue macrophages showed distinct expression patterns at different bone regions. RANKL was found in close proximity to inducible nitric oxide synthase-positive （iNOS＋） cells in vivo, suggesting an association between RANKL expression and iNOS＋ cells, especially in trabecular bone. RANKL-induced macrophages showed a different cytokine secretion profile compared with pathologically induced macrophages. Both osteoclasts and M1 macrophages peaked on day 7 during bone healing. RANKL could trigger Ml-like macrophages with properties that were different from those of LPS＋IFN-γ-induced macrophages. These RANKL-activated M1 macrophages were actively involved in bone formation.
基金This study was supported by the National Natural Science Foundation of China(Grant No.31971843)the Technology System of Modern Agricultural Industry in Guangdong(Grant No.2020KJ105)Guangzhou Science and Technology Project in China(Grant No.202103000075).
文摘Epoxiconazole is a triazole compound.However,the effects of epoxiconazole on crop productivity and quality were rarely reported.In this study,we investigated the effects of epoxiconazole application on yield formation,grain quality attributes,and 2-acetyl-1-pyrroline(2-AP)content in fragrant rice.A three-year field experiment was carried out with a fragrant rice variety,Meixiangzhan 2.At the heading stage,0,0.02,0.04,0.08,0.16 and 0.32 g/L epoxiconazole solutions were foliar applied to fragrant rice plants,respectively.The results showed that epoxiconazole application significantly increased grain yield,seed-setting rate and 1000-grain weight.Chlorophyll content and net photosynthetic rate of fragrant rice during the grain-filling stage significantly increased due to epoxiconazole application.Foliar application of epoxiconazole at 0.08 g/L increased grain protein content and decreased both chalky rice rate and chalkiness area ratio of fragrant rice.Epoxiconazole also substantially increased grain 2-AP content by inducing the regulation in contents of related synthetic precursors,including proline,pyrroline-5-carboxylic acid,Δ1-pyrroline and methylglyoxal.Overall,foliar application of epoxiconazole could be used for the improvement in grain yield,grain quality and 2-AP content in fragrant rice production when applied concentration at 0.08-0.32 g/L.Our findings provided the new roles of epoxiconazole in crop production.
基金supported by the National Basic Research Program of China(Grant No.2012CB822002)the National Natural Science Foundation of China(Grant No.41202072)the "Key Laboratory of Northeast Asia biological evolution and environment of Ministry of Education" platform base construction project
文摘The Sifangtai and Mingshui formations were continuously cored in the SK-1 n borehole（China Cretaceous Continental Scientific Drilling-SongKe1-the north borehole）.The core is 767.96 m long,and the recovery is 94.7%.The ages of the formations range from middle Campanian to Danian.The sequence and process of lithology-lithofacies and cyclic stratigraphy were described in detail.Eight litho-types compose the Sifangtai Formation,and 15 litho-types compose the Mingshui Formation.Deposition was predominantly in meandering river and lacustrine environments,including 10 microfacies in the Sifangtai Formation and 15 microfacies in the Mingshui Formation.The complete sequence is composed of 535 m-scale cycles（sixth-order cycle）,152 fifth-order cycles,42 fourth-order cycles and five third-order cycles.The centimeter-scale description of the section revealed some previously unknown horizons such as a special type of mudstone,marl,volcanic ash and favorable sand reservoirs in the formations.The new-found evidence is very important for the interpretation of the evolution of the basin,conditions such as lake oxic events,the K/Pg boundary,tectonism in the late sag basin stage,and the reservoir-cap rock assemblages in the shallow stratigraphy.
基金supported by the National Natural Science Foundation of China ( 31570940,81772017)
文摘Mechanical stimuli play an essential role in maintaining bone remodeling and skeletal integrity.Meanwhile,bone can respond to the changes of mechanical condition to adjust its mass and architecture.Clinical studies discover that bedridden patients showed osteoporotic T-scores and low bone mineral density,and long-term immobilized patients presented reduced markers of bone formation.However,as bone formation mediated by osteoblast differentiation is a complex process,the underlying molecular mechanism of mechanical stimuli regulating bone formation is still unclear.Recent evidences show that microRNAs(miRNAs)are involved in mechanical stimuli regulating bone formation or osteoblast differentiation.Nevertheless,no direct evidence identifies mechanoresponsive miRNA in both human and animal bones,and clarifies its mechanoresponsive role under different mechanical conditions(e.g.mechanical unloading,reloading,loading).In the current study,we screened for differentially expressed miRNAs in bone specimens of bedridden patients with fractures,then identified that the expression of miR-138-5p,but not the other miRNAs,altered withbedridden time and was negatively correlated with the expression of the bone formation marker genes Alp(alkaline phosphatase).Moreover,miR-138-5p was up-regulated with reduced bone formation during unloading and down-regulated with increased bone formation during reloading in hind4imb unloaded mice.In addition,miR-138-5p was verified to be responsive to different mechanical unloading condition and cyclic mechanical stretch condition in primary osteogenic cells,respectively.Further in vitro data suggested that mechanoresponsive miR-138-5p directly targeted microtubule actin crosslinking factor 1(MACF1)to inhibit osteoblast differentiation.In vivo,we constructed an osteoblastic miR-138-5p transgenic mice model(TG138)with the Runx2promoter,and found that overexpression miR-138-5p supressed bone formation.Moreover,osteoblast-targeted inhibition of miR-138-5p sensitized bone anabolic response to mechanical loading in TG138 mice.Predominantly,the osteoblast-targeted inhibition of miR-138-5p could counteract bone formation reduction induced by hind limb unloading.Taken together,the mechanoresponsive miR-138-5p inhibited bone anabolic response for developing a novel bone anabolic sensitization strategy.
基金supported by the National Natural Science Foundation of China,No.31800892(to JLZ)the Natural Science Foundation of Guangdong Province of China,No.2018A030310254(to YY)a grant from Guangzhou Medical University Start-up Project of China,No.B195002002048(to JLZ)。
文摘The formation of nerve bundles,which is partially regulated by neural cell adhesion molecule 1(NCAM1),is important for neural network organization during peripheral nerve regeneration.However,little is known about how the extracellular matrix(ECM)microenvironment affects this process.Here,we seeded dorsal root ganglion tissue blocks on different ECM substrates of peripheral nerve ECM-derived matrixgel,Matrigel,laminin 521,collagen I,and collagen IV,and observed well-aligned axon bundles growing in the peripheral nerve ECM-derived environment.We confirmed that NCAM1 is necessary but not sufficient to trigger this phenomenon.A protein interaction assay identified collagen VI as an extracellular partner of NCAM1 in the regulation of axonal fasciculation.Collagen VI interacted with NCAM1 by directly binding to the FNIII domain,thereby increasing the stability of NCAM1 at the axolemma.Our in vivo experiments on a rat sciatic nerve defect model also demonstrated orderly nerve bundle regeneration with improved projection accuracy and functional recovery after treatment with 10 mg/m L Matrigel and 20μg/m L collagen VI.These findings suggest that the collagen VI-NCAM1 pathway plays a regulatory role in nerve bundle formation.This study was approved by the Animal Ethics Committee of Guangzhou Medical University(approval No.GY2019048)on April 30,2019.
基金This research was financially supported by the National Science and Technology Major Project(2016ZX05034)project of China Gelogical Survey(DD20160181).
文摘In order to evaluate the geological characteristics and gas-bearing factors of Niutitang Formation within the Lower Cambrian of northern Guizhou,the Huangping area located at the southern edge of the ancient uplift belt of Xuefeng Mountain was selected as the target area,and Well Huangdi 1 was drilled for the geological survey of shale gas.Through geological background analysis and well logging and laboratory analysis such as organic geochemical test,gas content analysis,isothermal adsorption,and specific surface area experiments on Well Huangdi 1,the results show that the Niutitang Formation is a deep-water shelf,trough-like folds and thrust fault.The thickness of black shale is 119.95 m,of which carbonaceous shale is 89.6 m.The average value of organic carbon content is 3.55%,kerogen vitrinite reflectance value is 2.37% and kerogen type is sapropel-type.The brittle mineral content is 51%(quartz 38%),clay mineral content is 38.3%.The value of porosity and permeability are 0.5%and 0.0014 mD,which the reservoir of the Niutitang Formation belongs to low permeability with characteristics of ultra-low porosity.The gas content is 0.09‒1.31 m^3/t with a high-value area and a second high-value area.By comparing with the geological parameters of adjacent wells in the adjacent area,the accumulation model of“sediment control zone,Ro control zone,structure controlling reservoir”in the study area is proposed.Therefore,deep-water shelf-slope facies,Ro is between high maturity-early stage of overmaturity and well-preserved zones in the Niutitang Formation in this area are favorable direction for the next step of shale gas exploration.
文摘The inhibitory effect of parvovirus H-1 on the colonyforming ability in vitro of QGY-7703, a cultured human hepatoma cell line, and on the formation and growth of its tumors in nude mice was studied. With higher multiplicity of infection (MOI) of H-1 given, survival of the QGY-7703 cells was found to be decreased. H-1 DNA amplification level at 30 h postinfection(p.i.) was detected to be 7.4 times higher than that at 2 h by dispersed cells assay, while the cells were delayed to enter into S phase.Plaques were formed in the indicator cells (new-born human kidney cell line, NBK) by progeny H-1 virus particles released from the infected QGY-7703 cells by infectious cell center assay. The formation of tumors in nude mice by QGY-7703 cells which were injected s c at 2 h postinfection was observed to be prevented in 2 groups with given MOI 25 and 50. The tumor growth of MOI 10 group occurred at a lower exponential rate than that of control,after a 20 d latent period. It was evident that parvovirus H-1 exhibited a direct inhibitory effect on the formation and growth of human hepatoma cells in vivo as well as in vitro.
基金This work was supported by grants from the Key R&D Program of Jiangsu Province(Grant No.2017CX010)the National Natural Science Foundation of China(Grant No.81973308)to J.G.the Nanjing Science and Technology Development Program(Grant No.201402021)to H.L.
文摘This study aimed to investigate the effect and mechanism of valproic acid(VPA)on the neurosphere formation in rat embryonic cortical cells.We used free-floating neurosphere formation as a model system to evaluate the VPA on the proliferation of neural stem cells(NSCs).We found a time-and dose-dependent increase in neurosphere formation and NSC proliferation after VPA treatment.Further RNA-seq analysis demonstrated that the upregulated TGFβ1 signaling might attribute to the effect of VPA on the neurosphere formation and NSC proliferation.Consistently,the neurosphere formation and NSC proliferation were blocked by the treatment with SB431542,an inhibitor of TGFβ1 receptor.Moreover,in a coculture system,NSCs treated with VPA significantly reduced the oxygen-glucose deprivation-induced neuronal apoptosis.Taken together,our results showed that VPA could enhance neurosphere formation and NSC proliferation by activating TGFβ1,which might be a novel therapeutic strategy for neurological disorders.
