Objective: To reveal the mechanism of Mahuang Lianqiao Chixiaodou decoction and its disassembled formula for improving the skin barrier function in a mouse model of atopic dermatitis(AD).Methods: Sixty specific-pathog...Objective: To reveal the mechanism of Mahuang Lianqiao Chixiaodou decoction and its disassembled formula for improving the skin barrier function in a mouse model of atopic dermatitis(AD).Methods: Sixty specific-pathogen free male BALB/c mice were randomly divided into the control group,model group, whole formula group(WF), exterior-releasing formula group(ERF), interior-clearing formula group(ICF), and positive control group(PC). A mouse model of AD was established using the semiantigen 2,4-dinitrofluorobenzene induction method. The lesion scores, transepidermal water loss and p H, and skin histopathology of mice in each group were observed. The expressions of filaggrin, loricrin,and involucrin were detected by the streptavidin peroxidase immunohistochemical method and western blotting, and their mRNA expressions were detected by quantitative polymerase chain reaction.Results: Mice in the WF, ERF, ICF, and PC groups showed reduced skin lesion performance, improved histopathology, decreased skin lesion score, transepidermal water loss and pH, and upregulated expressions of proteins including filaggrin, loricrin, and involucrin, and their mRNAs. The most obvious regulatory effect was observed in the WF group, followed by the ICF, ERF, and PC groups, accordingly.Conclusions: Mahuang Lianqiao Chixiaodou decoction and its disassembled formula can improve the skin barrier function in a mouse model of AD by upregulating filaggrin, loricrin, and involucrin, and their mRNA expressions, and the most optimal effect was noted in the WF group, followed by the ICF and ERF groups, which suggests that the effect of clearing heat and resolving dampness in improving the skin barrier function of AD is more obvious and is one of the key treatments for AD.展开更多
Flow characteristics of thermally stratified shear flow in braided rivers are particularly complicated and poorly understood. In this study, a series of typical flow patterns was examined and their critical criteria w...Flow characteristics of thermally stratified shear flow in braided rivers are particularly complicated and poorly understood. In this study, a series of typical flow patterns was examined and their critical criteria were determined. Four flow patterns were identified: mixed, locally unstable, continuously stratified, and two-layer flow. Temperature distributions of the four types of flow patterns were analyzed and compared.The critical Froude numbers for unstable flow, FDcr1, and stable flow, FDcr2, were determined to be 6 and 1, respectively, and comparison of FDcr1 and FDcr2 to the peak Froude numbers, FD1 at the outer bank and FD2 at the inner bank along the anabranch, allowed the flow patterns to be assessed. Then, a discriminant based on initial Jeffreys-Keulegan stability parameters was established to distinguish the flow stages from twolayer flow to completely mixed flow. It is indicated that the three critical Jeffreys-Keulegan parameters increased with the diversion angle of braided rivers. Results also show that, compared to the stratified flow in straight and curved channels, it was more difficult for braided stratified flow to maintain as two-layer flow, and it more easily became mixed flow. Consequently, empirical expressions for stability criteria of the thermally stratified shear flow in braided rivers are presented.展开更多
基金supported by the Natural Science Foundation of Beijing Municipality Surface Project(7192114)。
文摘Objective: To reveal the mechanism of Mahuang Lianqiao Chixiaodou decoction and its disassembled formula for improving the skin barrier function in a mouse model of atopic dermatitis(AD).Methods: Sixty specific-pathogen free male BALB/c mice were randomly divided into the control group,model group, whole formula group(WF), exterior-releasing formula group(ERF), interior-clearing formula group(ICF), and positive control group(PC). A mouse model of AD was established using the semiantigen 2,4-dinitrofluorobenzene induction method. The lesion scores, transepidermal water loss and p H, and skin histopathology of mice in each group were observed. The expressions of filaggrin, loricrin,and involucrin were detected by the streptavidin peroxidase immunohistochemical method and western blotting, and their mRNA expressions were detected by quantitative polymerase chain reaction.Results: Mice in the WF, ERF, ICF, and PC groups showed reduced skin lesion performance, improved histopathology, decreased skin lesion score, transepidermal water loss and pH, and upregulated expressions of proteins including filaggrin, loricrin, and involucrin, and their mRNAs. The most obvious regulatory effect was observed in the WF group, followed by the ICF, ERF, and PC groups, accordingly.Conclusions: Mahuang Lianqiao Chixiaodou decoction and its disassembled formula can improve the skin barrier function in a mouse model of AD by upregulating filaggrin, loricrin, and involucrin, and their mRNA expressions, and the most optimal effect was noted in the WF group, followed by the ICF and ERF groups, which suggests that the effect of clearing heat and resolving dampness in improving the skin barrier function of AD is more obvious and is one of the key treatments for AD.
基金supported by the National Natural Science Foundation of China(Grants No.51379058,51379060,and 51479064)the Priority Academic Program Development of Jiangsu Higher Education Institutions(PAPD Project)the Fundamental Research Funds for the Central Universities(Grants No.2016B06714 and 2014B07814)
文摘Flow characteristics of thermally stratified shear flow in braided rivers are particularly complicated and poorly understood. In this study, a series of typical flow patterns was examined and their critical criteria were determined. Four flow patterns were identified: mixed, locally unstable, continuously stratified, and two-layer flow. Temperature distributions of the four types of flow patterns were analyzed and compared.The critical Froude numbers for unstable flow, FDcr1, and stable flow, FDcr2, were determined to be 6 and 1, respectively, and comparison of FDcr1 and FDcr2 to the peak Froude numbers, FD1 at the outer bank and FD2 at the inner bank along the anabranch, allowed the flow patterns to be assessed. Then, a discriminant based on initial Jeffreys-Keulegan stability parameters was established to distinguish the flow stages from twolayer flow to completely mixed flow. It is indicated that the three critical Jeffreys-Keulegan parameters increased with the diversion angle of braided rivers. Results also show that, compared to the stratified flow in straight and curved channels, it was more difficult for braided stratified flow to maintain as two-layer flow, and it more easily became mixed flow. Consequently, empirical expressions for stability criteria of the thermally stratified shear flow in braided rivers are presented.
