本研究根据FBJ骨肉瘤原癌基因同源物B(FosB)在动物母性行为发生过程中的重要作用,选择其作为影响母猪母性行为的候选基因,以白色杜洛克×二花脸F2资源群体为材料,分析了FosB基因在此群体中的遗传变异及其与分娩母猪母性行为的相关...本研究根据FBJ骨肉瘤原癌基因同源物B(FosB)在动物母性行为发生过程中的重要作用,选择其作为影响母猪母性行为的候选基因,以白色杜洛克×二花脸F2资源群体为材料,分析了FosB基因在此群体中的遗传变异及其与分娩母猪母性行为的相关性。采用PCR产物直接测序法搜寻了FosB基因4888、06 bp 2个片段的单核苷酸多态位点(SNPs),鉴别到FosB-EU163403-g.187A>T,FosB-EU163403-g.188A>C和FosB-EU163403-g.297C>G 3个SNPs。采用PCR-MbiI-RFLP法分析了G297C位点在资源群体中的多态及与母猪母性行为的相关性。传递不平衡检测(TDT)表明该多态位点与母猪杀婴行为、衔草做窝、哺乳、踩仔猪、压仔猪等母性行为均无显著相关性(P>0.364)。FBAT分析结果也证实了这一点(P>0.236)。考虑到母性行为属于复杂性状,可能由微效多基因控制,因此有必要在更大规模群体进行分析或搜寻其他候选基因的突变位点。展开更多
Objective To investigate the effect of M5 muscarinic receptor subtype on the locomotor sensitization induced by heroin priming, and it＇s effect on the FosB expression in the nucleus accumbens （NAc） and the hippocam...Objective To investigate the effect of M5 muscarinic receptor subtype on the locomotor sensitization induced by heroin priming, and it＇s effect on the FosB expression in the nucleus accumbens （NAc） and the hippocampus in the heroin sensitized rats. Methods Locomotor activity was measured every 10 min for 1 h after subcutaneous injection of heroin. FosB expression was assayed by immunohistochemistry, and the antisense oligonucleotides （AS-ONs） targeting M5 muscarinic receptor was transferred with the lipofectin. Results Microinjection of AS-ONs targeting M5 muscarinic receptor in the ventral tegmental area （VTA） blocked the expression of behavioral sensitization induced by heroin priming in rats. Meanwhile, the expression of FosB-positive neurons in either the NAc or the dentate gyrus （DG） of the hippocam- pus increased in heroin-induced locomotor sensitized rats. The enhancement of FosB-positive neurons in the NAc or DG could be inhibited by microinjection of M5 muscarinic receptor AS-ONs into the VTA before the heroin-induced locomotor sensitization was performed. In contrast, microinjection of M5 muscarinic receptor sense oligonucleotide （S-ONs） into the VTA did not block the expression of behavioral sensitization or the expression of FosB in the NAc or DG in the heroin sensitized rats. Conclusion Blocking M5 muscarinic receptor in the VTA inhibits the expression of heroin-induced locomotor sensitization, which is associated with the regulation of FosB expression in the NAc and hippocampus neurons. M5 muscarinic receptor may be a useful pharmacological target for the treatment of heroin addiction.展开更多
文摘本研究根据FBJ骨肉瘤原癌基因同源物B(FosB)在动物母性行为发生过程中的重要作用,选择其作为影响母猪母性行为的候选基因,以白色杜洛克×二花脸F2资源群体为材料,分析了FosB基因在此群体中的遗传变异及其与分娩母猪母性行为的相关性。采用PCR产物直接测序法搜寻了FosB基因4888、06 bp 2个片段的单核苷酸多态位点(SNPs),鉴别到FosB-EU163403-g.187A>T,FosB-EU163403-g.188A>C和FosB-EU163403-g.297C>G 3个SNPs。采用PCR-MbiI-RFLP法分析了G297C位点在资源群体中的多态及与母猪母性行为的相关性。传递不平衡检测(TDT)表明该多态位点与母猪杀婴行为、衔草做窝、哺乳、踩仔猪、压仔猪等母性行为均无显著相关性(P>0.364)。FBAT分析结果也证实了这一点(P>0.236)。考虑到母性行为属于复杂性状,可能由微效多基因控制,因此有必要在更大规模群体进行分析或搜寻其他候选基因的突变位点。
基金This work was supported by the National Nature Science Foundation of China （No.30470554）the National Basic Research Development Program of China（No.2003CB515404）.
文摘Objective To investigate the effect of M5 muscarinic receptor subtype on the locomotor sensitization induced by heroin priming, and it＇s effect on the FosB expression in the nucleus accumbens （NAc） and the hippocampus in the heroin sensitized rats. Methods Locomotor activity was measured every 10 min for 1 h after subcutaneous injection of heroin. FosB expression was assayed by immunohistochemistry, and the antisense oligonucleotides （AS-ONs） targeting M5 muscarinic receptor was transferred with the lipofectin. Results Microinjection of AS-ONs targeting M5 muscarinic receptor in the ventral tegmental area （VTA） blocked the expression of behavioral sensitization induced by heroin priming in rats. Meanwhile, the expression of FosB-positive neurons in either the NAc or the dentate gyrus （DG） of the hippocam- pus increased in heroin-induced locomotor sensitized rats. The enhancement of FosB-positive neurons in the NAc or DG could be inhibited by microinjection of M5 muscarinic receptor AS-ONs into the VTA before the heroin-induced locomotor sensitization was performed. In contrast, microinjection of M5 muscarinic receptor sense oligonucleotide （S-ONs） into the VTA did not block the expression of behavioral sensitization or the expression of FosB in the NAc or DG in the heroin sensitized rats. Conclusion Blocking M5 muscarinic receptor in the VTA inhibits the expression of heroin-induced locomotor sensitization, which is associated with the regulation of FosB expression in the NAc and hippocampus neurons. M5 muscarinic receptor may be a useful pharmacological target for the treatment of heroin addiction.
