In the present paper, the authors expound the approaches of four-gate points on treatment of diseases and the relevant theoretic evidence. The analysis and discussion were carried on in the aspects of the origins of f...In the present paper, the authors expound the approaches of four-gate points on treatment of diseases and the relevant theoretic evidence. The analysis and discussion were carried on in the aspects of the origins of four-gate points and the relationships of four-gate points with primary qi, running course of meridian, qi and blood and biaoben qijie of meridian and collateral. Being the major points in clinic, four-gate points provide extensive indications and good therapeutic effects, which are supported thoroughly by the theoretic evidence.展开更多
目的探讨骨髓增生异常综合征(MDS)患者CD34阳性(CD34+)细胞免疫表型特征。方法应用一组系列相关单克隆抗体和四色流式细胞术二次设门策略,对20例MDS患者的骨髓细胞悬液CD34+细胞进行免疫表型分析。结果随着MDS的进展[难治性贫血/难治性...目的探讨骨髓增生异常综合征(MDS)患者CD34阳性(CD34+)细胞免疫表型特征。方法应用一组系列相关单克隆抗体和四色流式细胞术二次设门策略,对20例MDS患者的骨髓细胞悬液CD34+细胞进行免疫表型分析。结果随着MDS的进展[难治性贫血/难治性贫血伴环状铁粒幼细胞→难治性贫血伴原始细胞增多→转化型原始细胞增多性难治性贫血(RA/RAS→RAEB→RAEBT)],在原始细胞群中的CD34+细胞比例逐渐增高(7.6%→40.1%→71.3%),各亚型之间有显著性差异(P<0.05)。在CD45 vs CD34散点图上二次设门取CD34+原始细胞行抗原表达的分析,异常表达HLA-DR及髓系抗原CD13、CD33和CD117、CD15比例减低。CD34在各亚型间有显著性差异(P<0.05)。结论二次设门策略能反映MDS原始细胞的免疫表型特征。展开更多
基金The study is subsidized by Specific Clinical Research on Chinese Medicine of Sciences and Technology, China Administration of Chinese Medicine(04-05LQ05)
文摘In the present paper, the authors expound the approaches of four-gate points on treatment of diseases and the relevant theoretic evidence. The analysis and discussion were carried on in the aspects of the origins of four-gate points and the relationships of four-gate points with primary qi, running course of meridian, qi and blood and biaoben qijie of meridian and collateral. Being the major points in clinic, four-gate points provide extensive indications and good therapeutic effects, which are supported thoroughly by the theoretic evidence.
文摘目的探讨骨髓增生异常综合征(MDS)患者CD34阳性(CD34+)细胞免疫表型特征。方法应用一组系列相关单克隆抗体和四色流式细胞术二次设门策略,对20例MDS患者的骨髓细胞悬液CD34+细胞进行免疫表型分析。结果随着MDS的进展[难治性贫血/难治性贫血伴环状铁粒幼细胞→难治性贫血伴原始细胞增多→转化型原始细胞增多性难治性贫血(RA/RAS→RAEB→RAEBT)],在原始细胞群中的CD34+细胞比例逐渐增高(7.6%→40.1%→71.3%),各亚型之间有显著性差异(P<0.05)。在CD45 vs CD34散点图上二次设门取CD34+原始细胞行抗原表达的分析,异常表达HLA-DR及髓系抗原CD13、CD33和CD117、CD15比例减低。CD34在各亚型间有显著性差异(P<0.05)。结论二次设门策略能反映MDS原始细胞的免疫表型特征。
