[ Objective] To establish an indirect ELISA method which can detect fowl adenovirus group I （FAVI） antibody easily and rapidly. [ Method] The expressed and purified FAVI penton recombinant protein was used to be an ...[ Objective] To establish an indirect ELISA method which can detect fowl adenovirus group I （FAVI） antibody easily and rapidly. [ Method] The expressed and purified FAVI penton recombinant protein was used to be an antigen, optimized the reaction conditions, and then estab- lished the FAVI indirect penton-ELISA antibody detection method. [ Result] The optimal coating concentration of antigen was 1.5 μg/hole, the opti- mal coating condition was 37℃ 2 h and 4 ℃ overnight; the optimal dilution of serum was 1：100; the optimal working concentration of anti-chicken IgG-HRP was 1：2 000; the positive and negative critical value of ELISA was 0.335. Detected the 100 chicken serum samples by the established penton-ELISA method, the positive rate was 41%. [ Conclusion] Through the study, ~e established penton-ELISA method has a good specificity, sensitivity and reproducibility. And it offers an effective tool for the diagnosis of FAVI, the survey of antibody and epidemiology survey.展开更多
基金Guangxi Expert Special Fund Project (2011B020)Guangxi Science and Technology Research (0815009-3-6 and 10100014-5 )Guangxi Natural Science Foundation (2010GXNSFA013090)
文摘[ Objective] To establish an indirect ELISA method which can detect fowl adenovirus group I （FAVI） antibody easily and rapidly. [ Method] The expressed and purified FAVI penton recombinant protein was used to be an antigen, optimized the reaction conditions, and then estab- lished the FAVI indirect penton-ELISA antibody detection method. [ Result] The optimal coating concentration of antigen was 1.5 μg/hole, the opti- mal coating condition was 37℃ 2 h and 4 ℃ overnight; the optimal dilution of serum was 1：100; the optimal working concentration of anti-chicken IgG-HRP was 1：2 000; the positive and negative critical value of ELISA was 0.335. Detected the 100 chicken serum samples by the established penton-ELISA method, the positive rate was 41%. [ Conclusion] Through the study, ~e established penton-ELISA method has a good specificity, sensitivity and reproducibility. And it offers an effective tool for the diagnosis of FAVI, the survey of antibody and epidemiology survey.
