Purpose:To investigate the genotyping of Foxp3-3279(A /C,rs376158) genes in patients with dust mite-induced allergic conjunctivitis from Guangdong province and to explore the association between these genes and the su...Purpose:To investigate the genotyping of Foxp3-3279(A /C,rs376158) genes in patients with dust mite-induced allergic conjunctivitis from Guangdong province and to explore the association between these genes and the susceptibility to dust mite allergic conjunctivitis.Methods:.In total,.80 patients with dust mite allergic conjunctivitis and 103 healthy Han Chinese were enrolled in the study and received genotyping of Foxp3-3279(A / C,rs376158)by PCR-SSP technique.Results: Genotype frequency of Foxp3-3279 AA,.CA,.and CC in patients with dust mite allergic conjunctivitis were 1.25%,25.00% and 73.75%,respectively. Gene frequency of C and A in patients with dust mite allergic conjunctivitis were 86.25%and 13.75% with no significant difference from healthy counterparts(both P>0.05).Conclusion:.Foxp3-3279 polymorphisms did not significantly differ between patients with dust mite allergic conjunctivitis and healthy controls, implying that this genetic locus is probably not an independent risk factor of the underlying pathogenesis of dust mite allergic conjunctivitis.展开更多
目的:探讨叉状头转录因子(FoxP3)/T细胞免疫球蛋白黏蛋白分子-3(Tim-3)信号通路中的相关基因在系统性硬化症(SSc)中的表达。方法:收集我院30例系统性硬化症患者和15例健康对照的外周血,分离得到外周血单核细胞(PBMCs),流式细胞术检测PB...目的:探讨叉状头转录因子(FoxP3)/T细胞免疫球蛋白黏蛋白分子-3(Tim-3)信号通路中的相关基因在系统性硬化症(SSc)中的表达。方法:收集我院30例系统性硬化症患者和15例健康对照的外周血,分离得到外周血单核细胞(PBMCs),流式细胞术检测PBMCs中CD4+CD25+Treg细胞和Th17辅助细胞(Th17)变化,RT-PCR检测PBMCs中FoxP3和Tim-3mRNA变化,ELISA检测细胞毒T淋巴细胞相关抗原4(cytotoxic T lymphocyte-associated antigen-4,CTLA-4)及γ干扰素(IFN-γ)含量。结果:与健康对照组相比,SSc患者PBMCs中Treg细胞和Th17细胞数目上升,但二者比值下降,FoxP3mRNA表达量下降,而Tim3mRNA表达量上升;CTLA-4及IFN-γ含量皆下降。结论:SSc患者PBMCs中T调节细胞(Treg)和Th17水平提高,但功能上存在缺陷,可能与FoxP3/Tim-3信号通路中相关基因表达变化有关。展开更多
基金Science and Technology Planning Project of Guangdong Province,China(2010B031600139)
文摘Purpose:To investigate the genotyping of Foxp3-3279(A /C,rs376158) genes in patients with dust mite-induced allergic conjunctivitis from Guangdong province and to explore the association between these genes and the susceptibility to dust mite allergic conjunctivitis.Methods:.In total,.80 patients with dust mite allergic conjunctivitis and 103 healthy Han Chinese were enrolled in the study and received genotyping of Foxp3-3279(A / C,rs376158)by PCR-SSP technique.Results: Genotype frequency of Foxp3-3279 AA,.CA,.and CC in patients with dust mite allergic conjunctivitis were 1.25%,25.00% and 73.75%,respectively. Gene frequency of C and A in patients with dust mite allergic conjunctivitis were 86.25%and 13.75% with no significant difference from healthy counterparts(both P>0.05).Conclusion:.Foxp3-3279 polymorphisms did not significantly differ between patients with dust mite allergic conjunctivitis and healthy controls, implying that this genetic locus is probably not an independent risk factor of the underlying pathogenesis of dust mite allergic conjunctivitis.
文摘目的:探讨叉状头转录因子(FoxP3)/T细胞免疫球蛋白黏蛋白分子-3(Tim-3)信号通路中的相关基因在系统性硬化症(SSc)中的表达。方法:收集我院30例系统性硬化症患者和15例健康对照的外周血,分离得到外周血单核细胞(PBMCs),流式细胞术检测PBMCs中CD4+CD25+Treg细胞和Th17辅助细胞(Th17)变化,RT-PCR检测PBMCs中FoxP3和Tim-3mRNA变化,ELISA检测细胞毒T淋巴细胞相关抗原4(cytotoxic T lymphocyte-associated antigen-4,CTLA-4)及γ干扰素(IFN-γ)含量。结果:与健康对照组相比,SSc患者PBMCs中Treg细胞和Th17细胞数目上升,但二者比值下降,FoxP3mRNA表达量下降,而Tim3mRNA表达量上升;CTLA-4及IFN-γ含量皆下降。结论:SSc患者PBMCs中T调节细胞(Treg)和Th17水平提高,但功能上存在缺陷,可能与FoxP3/Tim-3信号通路中相关基因表达变化有关。