Modified constraint-induced movement therapy(mCIMT)has shown beneficial effects on motor function improvement after brain injury,but the exact mechanism remains unclear.In this study,amplitude of low frequency fluctua...Modified constraint-induced movement therapy(mCIMT)has shown beneficial effects on motor function improvement after brain injury,but the exact mechanism remains unclear.In this study,amplitude of low frequency fluctuation(ALFF)metrics measured by resting-state functional magnetic resonance imaging was obtained to investigate the efficacy and mechanism of mCIMT in a control co rtical impact(CCI)rat model simulating traumatic brain injury.At 3 days after control co rtical impact model establishment,we found that the mean ALFF(mALFF)signals were decreased in the left motor cortex,somatosensory co rtex,insula cortex and the right motor co rtex,and were increased in the right corpus callosum.After 3 weeks of an 8-hour daily mClMT treatment,the mALFF values were significantly increased in the bilateral hemispheres compared with those at 3 days postoperatively.The mALFF signal valu es of left corpus callosum,left somatosensory cortex,right medial prefro ntal cortex,right motor co rtex,left postero dorsal hippocampus,left motor cortex,right corpus callosum,and right somatosensory cortex were increased in the mCIMT group compared with the control cortical impact group.Finally,we identified brain regions with significantly decreased mALFF valu es at 3 days postoperatively.Pearson correlation coefficients with the right forelimb sliding score indicated that the improvement in motor function of the affected upper limb was associated with an increase in mALFF values in these brain regions.Our findings suggest that functional co rtical plasticity changes after brain injury,and that mCIMT is an effective method to improve affected upper limb motor function by promoting bilateral hemispheric co rtical remodeling.mALFF values correlate with behavio ral changes and can potentially be used as biomarkers to assess dynamic cortical plasticity after traumatic brain injury.展开更多
Objective:To explore the differences in the central response after acupuncture and moxibusbution at Zusanli(足三里ST36) in treatment of functional dyspepsia(FD) based on the analysis of fractional amplitude of low fre...Objective:To explore the differences in the central response after acupuncture and moxibusbution at Zusanli(足三里ST36) in treatment of functional dyspepsia(FD) based on the analysis of fractional amplitude of low frequency fluctuation(fALFF) of resting-state functional magnetic resonance imaging(rfMRI).Methods:A total of 60 patients with FD were randomized into an acupuncture group and a moxibustion group,30 cases in each one.In the acupuncture group,acupuncture was applied to bilateral ST36.In the moxibustion group,moxibustion was exerted at bilateral ST36.The treatment was given once a day,5 times a week,totally for 4 weeks in each group,including 20 treatments with acupuncture or moxibustion.Separately,before and at the end of treatment,rfMRI scanning was conducted in two groups.Using data processing assistant for resting-state fMRI(DPARSF) software and MATLAB data platform,rfMRI data were collected for preprocessing and fALFF analysis.Results:Compared with the data before treatment,after treated with acupuncture at ST36 in FD patients,fALFF value was reduced in the right superior frontal gyrus,left superior frontal gyrus,left inferior frontal gyrus,right cuneus,left precuneus,right middle occipital gyrus,left middle occipital gyrus,etc.,and it was increased in the left parahippocampus,right parahippocampus,left cerebellum,etc(all P <0.01).After treated with moxibustion,the remarkable increase of fALFF was not discovered in brain areas,but fALFF decreased significantly in the left superior parietal gyrus(P <0.01).Compared with moxibustion at ST36,after acupuncture at ST36 in FD patients,the increase of fALFF appeared in the right putamen and the decrease of it occurred in the middle occipital gyrus,indicating the statistical significance(both P <0.05).Conclusion:The differences in central function responses are induced in treamtent with acupuncture and moxibustion,which is probably related to the resting-state default network associated with targeted regulation and ventral attention of brain areas in functional dyspepsia.展开更多
目的:采用动态低频振幅技术(dALFF)结合监督学习模型探讨无先兆偏头痛(患者睡眠障碍)的神经影像机制。方法:收集52名确诊为无先兆偏头痛的患者,根据匹兹堡睡眠质量指数将其分为偏头痛伴睡眠障碍组(MwSD)与偏头痛不伴睡眠障碍组(MwoSD),...目的:采用动态低频振幅技术(dALFF)结合监督学习模型探讨无先兆偏头痛(患者睡眠障碍)的神经影像机制。方法:收集52名确诊为无先兆偏头痛的患者,根据匹兹堡睡眠质量指数将其分为偏头痛伴睡眠障碍组(MwSD)与偏头痛不伴睡眠障碍组(MwoSD),并同期招募40名年龄与性别相匹配的健康志愿者作为对照组。使用3.0 T MRI采集其T1WI和BOLD-fMRI图像,通过随机森林(RF)、逻辑回归(LR)、支持向量机(SVM)机器学习算法对其dALFF进行分析。结果:单因素方差分析(one-way ANOVA)显示3组受试者在左侧丘脑、右侧枕中回、左侧楔前叶dALFF有差异;两独立样本t检验显示MwSD组较MwoSD组左内侧和旁扣带回、左侧额中回dALFF值升高,右侧颞上回dALFF值下降;无监督学习模型中SVM性能较好,权重绝对值排名前三的脑区右侧枕中回、左侧楔前叶、左内侧和旁扣带回,均与视觉通路相关。结论:视觉通路相关脑区dALFF值可作为预测MwSD患者的神经影像学标志。展开更多
癫痫是一种慢性神经系统疾病,其分类广泛、机制复杂,具有反复发作和不可预测的特点,对患者的生活造成了一定的影响。深度了解其病理生理机制对疾病的治疗和提高患者生活质量十分重要,静息态功能磁共振成像(resting-state functional mag...癫痫是一种慢性神经系统疾病,其分类广泛、机制复杂,具有反复发作和不可预测的特点,对患者的生活造成了一定的影响。深度了解其病理生理机制对疾病的治疗和提高患者生活质量十分重要,静息态功能磁共振成像(resting-state functional magnetic resonance imaging,rs-fMRI)现已成为探究癫痫脑功能改变的有效方法。目前基于rs-fMRI研究癫痫的数据分析方法主要有低频振幅(amplitude of low frequency fluctuation,ALFF)、局部一致性(regional homogeneity,ReHo)、功能连接(function connection,FC)和图论分析。本文将对rs-fMRI的各种分析方法在癫痫中的应用作一综述,为癫痫的病理生理机制及术前定位、治疗提供重要参考。展开更多
目的初步探究肾虚证亚健康人群认知功能改变的神经影像学机制。方法对35例肾虚型亚健康受试者和35例健康对照进行重复性成套神经心理状态测验(Repeatable Battery for the Assessment of Neuropsychological Status,RBANS)及评估,并进...目的初步探究肾虚证亚健康人群认知功能改变的神经影像学机制。方法对35例肾虚型亚健康受试者和35例健康对照进行重复性成套神经心理状态测验(Repeatable Battery for the Assessment of Neuropsychological Status,RBANS)及评估,并进行静息态功能磁共振扫描。应用皮质分析方法计算两组被试脑区的低频震荡振幅(amplitude of low frequency fluctuations,ALFF)值和分数低频振幅(fractional ALFF,fALFF)值,并分析异常功能活动脑区与认知功能及临床症状之间的相关性。结果与健康受试者相比,肾虚型亚健康受试者的即刻记忆、注意编码及RBANS测验总分显著降低;并发现其左侧初级视觉皮质、左侧背侧枕叶皮质及右侧辅助运动皮质等脑区的ALFF/fALFF值异常改变。此外,左侧背侧枕叶皮质、右侧额叶眼动区ALFF值,左侧初级视觉皮质fALFF值与肾虚证积分均成正比。右侧额叶眼动区ALFF值,右侧辅助运动皮质fALFF值与测验总分成反比。结论肾虚型亚健康受试者的记忆与注意功能受损,左侧枕叶和右侧额叶等多个脑区神经活动的改变可能是肾虚证亚健康人群认知功能改变的潜在神经机制。展开更多
目的采用低频振幅(amplitude of low frequency fluctuation,ALFF)、比率低频振幅(fractional amplitude of low frequency fluctuations,fALFF)和功能连接(functional connectivity,FC)方法探究原发性痛经(primary dysmenorrhea,PDM)...目的采用低频振幅(amplitude of low frequency fluctuation,ALFF)、比率低频振幅(fractional amplitude of low frequency fluctuations,fALFF)和功能连接(functional connectivity,FC)方法探究原发性痛经(primary dysmenorrhea,PDM)患者疼痛的中枢机制。材料与方法纳入31例PDM患者,所有受试者均接受经期与围排卵期双期静息态功能磁共振成像检查。计算ALFF、fALFF值,提取ALFF和fALFF值都有差异的脑区作为感兴趣区(region of interest,ROI),计算ROI与全脑体素的FC值。采用配对样本t检验比较PDM患者疼痛与无痛状态下ALFF、fALFF、FC值,并采用皮尔逊相关分析探索PDM患者ALFF、fALFF值与临床指标的关系。结果与非疼痛期比较,PDM患者在疼痛期显示左侧内侧额上回、右侧小脑Ⅱ区ALFF值显著升高,左侧岛叶ALFF值显著降低(双尾检验P<0.05,Alphasim校正,团块大小>176);PDM患者疼痛期的左侧背外侧额上回、左侧额下回三角部、右侧豆状核的fALFF值显著高于非疼痛期,左侧楔前叶的fALFF值显著低于非疼痛期(双尾检验P<0.05,Alphasim校正,团块大小>154)。随后,基于种子点的FC分析显示,左侧额上回为ALFF和fALFF值都有差异的脑区,相较于非疼痛期,疼痛期其与左侧小脑Ⅷ区的连接性降低(双尾检验P<0.05,Alphasim校正,团块水平>181);Pearson相关分析显示PDM患者疼痛期的左侧岛叶ALFF值与CMSS-t呈负相关(r=−0.400,P=0.026)。结论本研究揭示了PDM存在ALFF与fALFF的异常改变,并涉及多个脑网络,为帮助理解PDM的中枢机制提供新见解。展开更多
目的基于静息态功能磁共振技术探究脑安滴丸治疗偏头痛的中枢机制。方法纳入19例偏头痛患者作为观察组,16例健康受试者作为对照组。观察组给予12周的脑安滴丸治疗,对照组不予任何干预。观察组在治疗前后进行静息态功能磁共振(resting st...目的基于静息态功能磁共振技术探究脑安滴丸治疗偏头痛的中枢机制。方法纳入19例偏头痛患者作为观察组,16例健康受试者作为对照组。观察组给予12周的脑安滴丸治疗,对照组不予任何干预。观察组在治疗前后进行静息态功能磁共振(resting state function magnetic resonance imaging,rs-fMRI)扫描,对照组仅在入组当天进行1次扫描。记录观察组治疗前后、随访4周头痛发作天数、头痛发作次数、头痛持续时间、视觉模拟评分(visual analogue scale,VAS)及患者报告的结局(patients-reported outcomes,PRO)量表评分。分析治疗前两组间的低频振幅(amplitude of low frequency fluctuation,ALFF)、局部一致性(regional homogeneity,ReHo)差异脑区,以ALFF、ReHo的共同差异脑区为感兴趣脑区(region of interest,ROI),分析计算观察组治疗前后ROI与全脑体素的功能连接(functional connectivity,FC)变化,并将FC的变化值与临床数据的改善值进行Pearson相关分析。结果观察组治疗后、随访4周的头痛发作天数、头痛发作次数、头痛持续时间及PRO量表评分均显著低于治疗前(P<0.05),且观察组随访4周的VAS评分亦显著降低(P<0.05);与对照组比较,治疗前观察组左侧背外侧额上回、右侧前扣带和旁扣带脑回、右侧岛叶、双侧内侧额上回、双侧额中回和左侧楔前叶的ALFF值显著降低(P<0.05),右侧中央前回的ReHo值显著升高(P<0.05),左侧楔前叶的ReHo值显著降低(P<0.05)。以左侧楔前叶为ROI,治疗后左侧楔前叶与左侧壳核的FC值较治疗前降低(P<0.05),未发现治疗前后FC的变化值与临床数据的相关性(P>0.05)。结论偏头痛患者存在认知、情感、感觉等多个脑区的功能活动异常,而脑安滴丸可能是通过调控楔前叶—壳核的FC达到治疗偏头痛的作用。展开更多
基金supported by the National Key R&D Program of China,Nos.2020YFC2004202(to DSX),2018 YFC2001600(to XYH)the National Natural Science Foundation of China,Nos.81974358(to DSX),81802249(to XYH)and 82172554(to XYH)。
文摘Modified constraint-induced movement therapy(mCIMT)has shown beneficial effects on motor function improvement after brain injury,but the exact mechanism remains unclear.In this study,amplitude of low frequency fluctuation(ALFF)metrics measured by resting-state functional magnetic resonance imaging was obtained to investigate the efficacy and mechanism of mCIMT in a control co rtical impact(CCI)rat model simulating traumatic brain injury.At 3 days after control co rtical impact model establishment,we found that the mean ALFF(mALFF)signals were decreased in the left motor cortex,somatosensory co rtex,insula cortex and the right motor co rtex,and were increased in the right corpus callosum.After 3 weeks of an 8-hour daily mClMT treatment,the mALFF values were significantly increased in the bilateral hemispheres compared with those at 3 days postoperatively.The mALFF signal valu es of left corpus callosum,left somatosensory cortex,right medial prefro ntal cortex,right motor co rtex,left postero dorsal hippocampus,left motor cortex,right corpus callosum,and right somatosensory cortex were increased in the mCIMT group compared with the control cortical impact group.Finally,we identified brain regions with significantly decreased mALFF valu es at 3 days postoperatively.Pearson correlation coefficients with the right forelimb sliding score indicated that the improvement in motor function of the affected upper limb was associated with an increase in mALFF values in these brain regions.Our findings suggest that functional co rtical plasticity changes after brain injury,and that mCIMT is an effective method to improve affected upper limb motor function by promoting bilateral hemispheric co rtical remodeling.mALFF values correlate with behavio ral changes and can potentially be used as biomarkers to assess dynamic cortical plasticity after traumatic brain injury.
基金Supported by Changsha Outstanding Innovation Youth Training Project:kq1905036Open Fund Project of First-Class Discipline in Traditional Chinese Medicine of Hunan University of Chinese Medicine:2018ZYX04。
文摘Objective:To explore the differences in the central response after acupuncture and moxibusbution at Zusanli(足三里ST36) in treatment of functional dyspepsia(FD) based on the analysis of fractional amplitude of low frequency fluctuation(fALFF) of resting-state functional magnetic resonance imaging(rfMRI).Methods:A total of 60 patients with FD were randomized into an acupuncture group and a moxibustion group,30 cases in each one.In the acupuncture group,acupuncture was applied to bilateral ST36.In the moxibustion group,moxibustion was exerted at bilateral ST36.The treatment was given once a day,5 times a week,totally for 4 weeks in each group,including 20 treatments with acupuncture or moxibustion.Separately,before and at the end of treatment,rfMRI scanning was conducted in two groups.Using data processing assistant for resting-state fMRI(DPARSF) software and MATLAB data platform,rfMRI data were collected for preprocessing and fALFF analysis.Results:Compared with the data before treatment,after treated with acupuncture at ST36 in FD patients,fALFF value was reduced in the right superior frontal gyrus,left superior frontal gyrus,left inferior frontal gyrus,right cuneus,left precuneus,right middle occipital gyrus,left middle occipital gyrus,etc.,and it was increased in the left parahippocampus,right parahippocampus,left cerebellum,etc(all P <0.01).After treated with moxibustion,the remarkable increase of fALFF was not discovered in brain areas,but fALFF decreased significantly in the left superior parietal gyrus(P <0.01).Compared with moxibustion at ST36,after acupuncture at ST36 in FD patients,the increase of fALFF appeared in the right putamen and the decrease of it occurred in the middle occipital gyrus,indicating the statistical significance(both P <0.05).Conclusion:The differences in central function responses are induced in treamtent with acupuncture and moxibustion,which is probably related to the resting-state default network associated with targeted regulation and ventral attention of brain areas in functional dyspepsia.
文摘目的:采用动态低频振幅技术(dALFF)结合监督学习模型探讨无先兆偏头痛(患者睡眠障碍)的神经影像机制。方法:收集52名确诊为无先兆偏头痛的患者,根据匹兹堡睡眠质量指数将其分为偏头痛伴睡眠障碍组(MwSD)与偏头痛不伴睡眠障碍组(MwoSD),并同期招募40名年龄与性别相匹配的健康志愿者作为对照组。使用3.0 T MRI采集其T1WI和BOLD-fMRI图像,通过随机森林(RF)、逻辑回归(LR)、支持向量机(SVM)机器学习算法对其dALFF进行分析。结果:单因素方差分析(one-way ANOVA)显示3组受试者在左侧丘脑、右侧枕中回、左侧楔前叶dALFF有差异;两独立样本t检验显示MwSD组较MwoSD组左内侧和旁扣带回、左侧额中回dALFF值升高,右侧颞上回dALFF值下降;无监督学习模型中SVM性能较好,权重绝对值排名前三的脑区右侧枕中回、左侧楔前叶、左内侧和旁扣带回,均与视觉通路相关。结论:视觉通路相关脑区dALFF值可作为预测MwSD患者的神经影像学标志。
文摘癫痫是一种慢性神经系统疾病,其分类广泛、机制复杂,具有反复发作和不可预测的特点,对患者的生活造成了一定的影响。深度了解其病理生理机制对疾病的治疗和提高患者生活质量十分重要,静息态功能磁共振成像(resting-state functional magnetic resonance imaging,rs-fMRI)现已成为探究癫痫脑功能改变的有效方法。目前基于rs-fMRI研究癫痫的数据分析方法主要有低频振幅(amplitude of low frequency fluctuation,ALFF)、局部一致性(regional homogeneity,ReHo)、功能连接(function connection,FC)和图论分析。本文将对rs-fMRI的各种分析方法在癫痫中的应用作一综述,为癫痫的病理生理机制及术前定位、治疗提供重要参考。
文摘目的初步探究肾虚证亚健康人群认知功能改变的神经影像学机制。方法对35例肾虚型亚健康受试者和35例健康对照进行重复性成套神经心理状态测验(Repeatable Battery for the Assessment of Neuropsychological Status,RBANS)及评估,并进行静息态功能磁共振扫描。应用皮质分析方法计算两组被试脑区的低频震荡振幅(amplitude of low frequency fluctuations,ALFF)值和分数低频振幅(fractional ALFF,fALFF)值,并分析异常功能活动脑区与认知功能及临床症状之间的相关性。结果与健康受试者相比,肾虚型亚健康受试者的即刻记忆、注意编码及RBANS测验总分显著降低;并发现其左侧初级视觉皮质、左侧背侧枕叶皮质及右侧辅助运动皮质等脑区的ALFF/fALFF值异常改变。此外,左侧背侧枕叶皮质、右侧额叶眼动区ALFF值,左侧初级视觉皮质fALFF值与肾虚证积分均成正比。右侧额叶眼动区ALFF值,右侧辅助运动皮质fALFF值与测验总分成反比。结论肾虚型亚健康受试者的记忆与注意功能受损,左侧枕叶和右侧额叶等多个脑区神经活动的改变可能是肾虚证亚健康人群认知功能改变的潜在神经机制。
文摘目的快速眼动睡眠行为障碍(rapid eye movement sleep behavior disorder,RBD)是帕金森病(Parkinson's disease,PD)常见的非运动症状且是重要预后因素。本研究通过静息态功能磁共振成像,利用度中心度(degreecentrality,DC)和低频振幅(amplitudes of low-frequency fluctuation,ALFF)分析PD伴RBD和不伴有RBD患者组以及健康对照组三组间DC值和ALFF值,探索PD伴RBD患者脑功能活动特征及RBD特异性脑区,探究RBD发生的病理机制。材料与方法招募20例伴有RBD的PD患者(PD-RBD组)、40例无RBD的PD患者(PD-nonRBD组)和44例年龄性别匹配的健康对照(健康对照组),三组被试均接受磁共振扫描。利用静息态数据计算DC值和ALFF值,探测脑功能特征。结果方差分析结果显示三组间DC值主效应脑区为右侧中央前回、颞上回、小脑、额中回(P<0.05,FDR校正);ALFF值主效应脑区为左侧海马旁回、楔叶、舌回(P<0.05,FDR校正)。进一步分析发现相比于PD-nonRBD组,PD-RBD患者表现为右侧额中回DC值升高(t=4.02;P=0.007,FDR校正);左侧楔前叶DC值降低(t=5.30;P=0.009,FDR校正)。相比于健康对照组,PD-RBD患者表现为左侧额上回、小脑、右侧颞上回、左侧颞中回、额中回的DC值升高(P<0.05,FDR校正);左侧中央前回、颞上回和颞中回的DC值降低(P<0.05,FDR校正);右侧楔叶ALFF值降低(P<0.05,FDR校正)。结论PD-RBD在DC和ALFF上有独特的影像学特征,特别是右侧额中回、左侧楔前叶的功能异常可能与PD患者RBD的发生密切相关。
文摘目的采用低频振幅(amplitude of low frequency fluctuation,ALFF)、比率低频振幅(fractional amplitude of low frequency fluctuations,fALFF)和功能连接(functional connectivity,FC)方法探究原发性痛经(primary dysmenorrhea,PDM)患者疼痛的中枢机制。材料与方法纳入31例PDM患者,所有受试者均接受经期与围排卵期双期静息态功能磁共振成像检查。计算ALFF、fALFF值,提取ALFF和fALFF值都有差异的脑区作为感兴趣区(region of interest,ROI),计算ROI与全脑体素的FC值。采用配对样本t检验比较PDM患者疼痛与无痛状态下ALFF、fALFF、FC值,并采用皮尔逊相关分析探索PDM患者ALFF、fALFF值与临床指标的关系。结果与非疼痛期比较,PDM患者在疼痛期显示左侧内侧额上回、右侧小脑Ⅱ区ALFF值显著升高,左侧岛叶ALFF值显著降低(双尾检验P<0.05,Alphasim校正,团块大小>176);PDM患者疼痛期的左侧背外侧额上回、左侧额下回三角部、右侧豆状核的fALFF值显著高于非疼痛期,左侧楔前叶的fALFF值显著低于非疼痛期(双尾检验P<0.05,Alphasim校正,团块大小>154)。随后,基于种子点的FC分析显示,左侧额上回为ALFF和fALFF值都有差异的脑区,相较于非疼痛期,疼痛期其与左侧小脑Ⅷ区的连接性降低(双尾检验P<0.05,Alphasim校正,团块水平>181);Pearson相关分析显示PDM患者疼痛期的左侧岛叶ALFF值与CMSS-t呈负相关(r=−0.400,P=0.026)。结论本研究揭示了PDM存在ALFF与fALFF的异常改变,并涉及多个脑网络,为帮助理解PDM的中枢机制提供新见解。
文摘目的基于静息态功能磁共振技术探究脑安滴丸治疗偏头痛的中枢机制。方法纳入19例偏头痛患者作为观察组,16例健康受试者作为对照组。观察组给予12周的脑安滴丸治疗,对照组不予任何干预。观察组在治疗前后进行静息态功能磁共振(resting state function magnetic resonance imaging,rs-fMRI)扫描,对照组仅在入组当天进行1次扫描。记录观察组治疗前后、随访4周头痛发作天数、头痛发作次数、头痛持续时间、视觉模拟评分(visual analogue scale,VAS)及患者报告的结局(patients-reported outcomes,PRO)量表评分。分析治疗前两组间的低频振幅(amplitude of low frequency fluctuation,ALFF)、局部一致性(regional homogeneity,ReHo)差异脑区,以ALFF、ReHo的共同差异脑区为感兴趣脑区(region of interest,ROI),分析计算观察组治疗前后ROI与全脑体素的功能连接(functional connectivity,FC)变化,并将FC的变化值与临床数据的改善值进行Pearson相关分析。结果观察组治疗后、随访4周的头痛发作天数、头痛发作次数、头痛持续时间及PRO量表评分均显著低于治疗前(P<0.05),且观察组随访4周的VAS评分亦显著降低(P<0.05);与对照组比较,治疗前观察组左侧背外侧额上回、右侧前扣带和旁扣带脑回、右侧岛叶、双侧内侧额上回、双侧额中回和左侧楔前叶的ALFF值显著降低(P<0.05),右侧中央前回的ReHo值显著升高(P<0.05),左侧楔前叶的ReHo值显著降低(P<0.05)。以左侧楔前叶为ROI,治疗后左侧楔前叶与左侧壳核的FC值较治疗前降低(P<0.05),未发现治疗前后FC的变化值与临床数据的相关性(P>0.05)。结论偏头痛患者存在认知、情感、感觉等多个脑区的功能活动异常,而脑安滴丸可能是通过调控楔前叶—壳核的FC达到治疗偏头痛的作用。
