The VEGF production by dedifferentiated chondrocytes under synovial fluid stimulation from coxarthrosis and femoral neck fracture patients

Objective To investigate the vascular endothelial growth factor(VEGF)expression level by chondrocytes isolated from patients with osteoarthritis (OA) in hip or femoral neck fracture (FNF) and explore the effect of synovial fluid from OA

Shale oil development techniques and application based on ternary-element storage and flow concept in Jiyang Depression,Bohai Bay Basin,East China

The ternary-element storage and flow concept for shale oil reservoirs in Jiyang Depression of Bohai Bay Basin,East China,was proposed based on the data of more than 10000 m cores and the production of more than 60 horizontal wells.The synergy of three elements(storage,fracture and pressure)contributes to the enrichment and high production of shale oil in Jiyang Depression.The storage element controls the enrichment of shale oil;specifically,the presence of inorganic pores and fractures,as well as laminae of lime-mud rocks,in the saline lake basin,is conducive to the storage of shale oil,and the high hydrocarbon generating capacity and free hydrocarbon content are the material basis for high production.The fracture element controls the shale oil flow;specifically,natural fractures act as flow channels for shale oil to migrate and accumulate,and induced fractures communicate natural fractures to form complex fracture network,which is fundamental to high production.The pressure element controls the high and stable production of shale oil;specifically,the high formation pressure provides the drive force for the migration and accumulation of hydrocarbons,and fracturing stimulation significantly increases the elastic energy of rock and fluid,improves the imbibition replacement of oil in the pores/fractures,and reduces the stress sensitivity,guaranteeing the stable production of shale oil for a long time.Based on the ternary-element storage and flow concept,a 3D development technology was formed,with the core techniques of 3D well pattern optimization,3D balanced fracturing,and full-cycle optimization of adjustment and control.This technology effectively guides the production and provides a support to the large-scale beneficial development of shale oil in Jiyang Depression.

A novel dandelion-based bionic proppant and its transportation mechanism in different types of fractures

Low-permeability reservoirs are generally characterized by low porosity and low permeability.Obtaining high production using the traditional method is technologically challenging because it yields a low reservoir recovery factor.In recent years,hydraulic fracturing technology is widely applied for efficiently exploiting and developing low-permeability reservoirs using a low-viscosity fluid as a fracturing fluid.However,the transportation of the proppant is inefficient in the low-viscosity fluid,and the proppant has a low piling-up height in fracture channels.These key challenges restrict the fluid(natural gas or oil)flow in fracture channels and their functional flow areas,reducing the profits of hydrocarbon exploitation.This study aimed to explore and develop a novel dandelion-bionic proppant by modifying the surface of the proppant and the fiber.Its structure was similar to that of dandelion seeds,and it had high transport and stacking efficiency in low-viscosity liquids compared with the traditional proppant.Moreover,the transportation efficiency of this newly developed proppant was investigated experimentally using six different types of fracture models(tortuous fracture model,rough fracture model,narrow fracture model,complex fracture model,large-scale single fracture model,and small-scale single fracture model).Experimental results indicated that,compared with the traditional proppant,the transportation efficiency and the packing area of the dandelion-based bionic proppant significantly improved in tap water or low-viscosity fluid.Compared with the traditional proppant,the dandelionbased bionic proppant had 0.1-4 times longer transportation length,0.3-5 times higher piling-up height,and 2-10 times larger placement area.The newly developed proppant also had some other extraordinary features.The tortuosity of the fracture did not influence the transportation of the novel proppant.This proppant could easily enter the branch fracture and narrow fracture with a high packing area in rough surface fractures.Based on the aforementioned characteristics,this novel proppant technique could improve the proppant transportation efficiency in the low-viscosity fracturing fluid and increase the ability of the proppant to enter the secondary fracture.This study might provide a new solution for effectively exploiting low-permeability hydrocarbon reservoirs.

Proppant transport in rough fractures of unconventional oil and gas reservoirs

A method to generate fractures with rough surfaces was proposed according to the fractal interpolation theory.Considering the particle-particle,particle-wall and particle-fluid interactions,a proppant-fracturing fluid two-phase flow model based on computational fluid dynamics(CFD)-discrete element method(DEM)coupling was established.The simulation results were verified with relevant experimental data.It was proved that the model can match transport and accumulation of proppants in rough fractures well.Several cases of numerical simulations were carried out.Compared with proppant transport in smooth flat fractures,bulge on the rough fracture wall affects transport and settlement of proppants significantly in proppant transportation in rough fractures.The higher the roughness of fracture,the faster the settlement of proppant particles near the fracture inlet,the shorter the horizontal transport distance,and the more likely to accumulate near the fracture inlet to form a sand plugging in a short time.Fracture wall roughness could control the migration path of fracturing fluid to a certain degree and change the path of proppant filling in the fracture.On the one hand,the rough wall bulge raises the proppant transport path and the proppants flow out of the fracture,reducing the proppant sweep area.On the other hand,the sand-carrying fluid is prone to change flow direction near the contact point of bulge,thus expanding the proppant sweep area.