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A 220 GHz Frequency-Division Multiplexing Wireless Link with High Data Rate
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作者 ZHANG Bo WANG Yihui +2 位作者 FENG Yinian YANG Yonghui PENG Lin 《ZTE Communications》 2023年第3期63-69,共7页
With the development of wireless communication,the 6G mobile communication technology has received wide attention.As one of the key technologies of 6G,terahertz(THz)communication technology has the characteristics of ... With the development of wireless communication,the 6G mobile communication technology has received wide attention.As one of the key technologies of 6G,terahertz(THz)communication technology has the characteristics of ultra-high bandwidth,high security and low environmental noise.In this paper,a THz duplexer with a half-wavelength coupling structure and a sub-harmonic mixer operating at 216 GHz and 204 GHz are designed and measured.Based on these key devices,a 220 GHz frequency-division multiplexing communication system is proposed,with a real-time data rate of 10.4 Gbit/s for one channel and a transmission distance of 15 m.The measured constellation diagram of two receivers is clearly visible,the signal-to-noise ratio(SNR)is higher than 22 dB,and the bit error ratio(BER)is less than 10^(−8).Furthermore,the high definition(HD)4K video can also be transmitted in real time without stutter. 展开更多
关键词 frequency-division multiplexing sub-harmonic mixer terahertz communication terahertz duplexer
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Joint nonlinearity and chromatic dispersion pre-compensation for coherent optical orthogonal frequency-division multiplexing systems
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作者 乔耀军 刘学君 纪越峰 《Chinese Physics B》 SCIE EI CAS CSCD 2011年第11期296-300,共5页
This paper introduces a joint nonlinearity and chromatic dispersion pre-compensation method for coherent optical orthogonal frequency-division multiplexing systems. The research results show that this method can reduc... This paper introduces a joint nonlinearity and chromatic dispersion pre-compensation method for coherent optical orthogonal frequency-division multiplexing systems. The research results show that this method can reduce the walk- off effect and can therefore equalize the nonlinear impairments effectively. Compared with the only other existing nonlinearity pre-compensation method, the joint nonlinearity and chromatic dispersion pre-compensation method is not only suitable for low-dispersion optical orthogonal frequency-division multiplexing system, but also effective for high- dispersion optical orthogonal frequency-division multiplexing transmission system with higher input power but without optical dispersion compensation. The suggested solution does not increase computation complexity compared with only nonlinearity pre-compensation method. For 40 Gbit/s coherent optical orthogonal frequency-division multiplexing 20 × 80 km standard single-mode fibre system, the suggested method can improve the nonlinear threshold (for Q 〉 10 dB) about 2.7, 1.2 and 1.0 dB, and the maximum Q factor about 1.2, 0.4 and 0.3 dB, for 2, 8 and 16 ps/(nm.km) dispersion coefficients. 展开更多
关键词 fibre optics NONLINEARITY chromatic dispersion coherent optical orthogonal frequency-division multiplexing
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Distributed Spectrum Sharing Scheme for Multiuser Orthogonal Frequency-Division Multiplexing Cognitive Radio Networks
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作者 YANG Long KUO Yonghong CHEN Jian ZHANG Hailin 《China Communications》 SCIE CSCD 2014年第1期79-91,共13页
This paper presents a novel spec- trum sharing design aiming at optimising the performance of a Multiuser Orthogonal Freq- uency-Division Multiplexing (MU-OFDM) Co- gnitive Radio Network (CRN) that consists of mul... This paper presents a novel spec- trum sharing design aiming at optimising the performance of a Multiuser Orthogonal Freq- uency-Division Multiplexing (MU-OFDM) Co- gnitive Radio Network (CRN) that consists of multiple secondary Transmitter-Receiver (Tx-Rx) pairs. For most MU-OFDM systems, the Exc- lusive Subchannel Assignment (ESA) is an efficient resource allocation method. Noneth- eless, it is inappropriate for the network consi- dered in this paper, because subchannels shar- ing among secondary Tx-Rx pairs can further improve the system performance. We investi- gate the Weighted Sum Rate (WSR) maximi- zation problem under the Shared Subchannel Assignment (SSA), where each subchannel is shared by multiple secondary Tx-Rx pairs. With Lagrangian duality technique, we decompose the original resource allocation problem into sev- eral sub-problems on each subchannel and pro- pose a duality-based suhchannel sharing ap- proach. For practical realisation in the cogni- tive systems without central control entity, a distributed duality-based WSR maximization scheme is presented. Simulation results mani- fest that the proposed scheme achieves sig- nificantly better performance than ESA duality scheme. 展开更多
关键词 cognitive radio networks Lagr- angian duality multiuser orthogonal frequen- cy-division multiplexing spectrum sharing weight sum rate maximization
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Optical Frequency Comb Frequency-division Multiplexing Dispersive Interference Multichannel Distance Measurement 被引量:1
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作者 Xu Liang Tengfei Wu +2 位作者 Jiarui Lin Linghui Yang Jigui Zhu 《Nanomanufacturing and Metrology》 EI 2023年第1期61-68,共8页
An optical frequency comb(OFC)frequency-division multiplexing dispersive interference multichannel distance measurement method is proposed.Based on the OFC dispersive interference,the wide OFC spectrum is divided into... An optical frequency comb(OFC)frequency-division multiplexing dispersive interference multichannel distance measurement method is proposed.Based on the OFC dispersive interference,the wide OFC spectrum is divided into multiple channels using a wavelength-division multiplexer.Under the existing light source and spectrometer,a single interference system can realize six channels of the high-precision parallel absolute distance measurement.The influence of the spectrum width and shape on the performance of the distance measurement channel is analyzed.The ranging accuracy of six channels is higher than±4μm under the optimization of a nonuniform discrete Fourier transform and Hanning window. 展开更多
关键词 frequency-division multiplexing Dispersive interference Absolute distance measurement Fourier transform
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Multi-dimensional multiplexing optical secret sharing framework with cascaded liquid crystal holograms 被引量:1
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作者 Keyao Li Yiming Wang +6 位作者 Dapu Pi Baoli Li Haitao Luan Xinyuan Fang Peng Chen Yanqing Lu Min Gu 《Opto-Electronic Advances》 SCIE EI CAS CSCD 2024年第1期28-35,共8页
Secret sharing is a promising technology for information encryption by splitting the secret information into different shares.However,the traditional scheme suffers from information leakage in decryption process since... Secret sharing is a promising technology for information encryption by splitting the secret information into different shares.However,the traditional scheme suffers from information leakage in decryption process since the amount of available information channels is limited.Herein,we propose and demonstrate an optical secret sharing framework based on the multi-dimensional multiplexing liquid crystal(LC)holograms.The LC holograms are used as spatially separated shares to carry secret images.The polarization of the incident light and the distance between different shares are served as secret keys,which can significantly improve the information security and capacity.Besides,the decryption condition is also restricted by the applied external voltage due to the variant diffraction efficiency,which further increases the information security.In implementation,an artificial neural network(ANN)model is developed to carefully design the phase distribution of each LC hologram.With the advantage of high security,high capacity and simple configuration,our optical secret sharing framework has great potentials in optical encryption and dynamic holographic display. 展开更多
关键词 holographic encryption optical secret sharing cascaded liquid crystal hologram multi-dimensional multiplexing
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Five-channel frequency-division multiplexing using low-loss epsilon-near-zero metamaterial waveguide
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作者 Binbin Hong Lei Sun +6 位作者 Wanlin Wang Yanbing Qiu Naixing Feng Dong Su Nutapong Somjit Ian Robertson Guo Ping Wang 《Science China(Physics,Mechanics & Astronomy)》 SCIE EI CAS CSCD 2022年第7期129-137,共9页
The rapidly growing global data usage has demanded more efficient ways to utilize the scarce electromagnetic spectrum resource. Recent research has focused on the development of efficient multiplexing techniques in th... The rapidly growing global data usage has demanded more efficient ways to utilize the scarce electromagnetic spectrum resource. Recent research has focused on the development of efficient multiplexing techniques in the millimeter-wave band(1-10 mm, or 30-300 GHz) due to the promise of large available bandwidth for future wireless networks. Frequency-division multiplexing is still one of the most commonly-used techniques to maximize the transmission capacity of a wireless network.Based on the frequency-selective tunnelling effect of the low-loss epsilon-near-zero metamaterial waveguide, we numerically and experimentally demonstrate five-channel frequency-division multiplexing and demultiplexing in the millimeter-wave range.We show that this device architecture offers great flexibility to manipulate the filter Q-factors and the transmission spectra of different channels, by changing of the epsilon-near-zero metamaterial waveguide topology and by adding a standard waveguide between two epsilon-near-zero channels. This strategy of frequency-division multiplexing may pave a way for efficiently allocating the spectrum for future communication networks. 展开更多
关键词 frequency-division multiplexing artificial effective medium epsilon-near-zero metamaterial integrated photonics
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Combining Polarization-Division Multiplexing and Ferromagnetic Nonreciprocity to Achieve In-Band Ultra-High Isolation for Full-Duplex Wireless Systems
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作者 Amir Afshani Ke Wu 《Engineering》 SCIE EI CAS CSCD 2024年第9期179-187,共9页
The in-band full-duplex(IBFD)wireless system is a promising candidate for 6G and beyond,as it can double data throughput and enormously lower transmission latency by supporting simultaneous in-band transmission and re... The in-band full-duplex(IBFD)wireless system is a promising candidate for 6G and beyond,as it can double data throughput and enormously lower transmission latency by supporting simultaneous in-band transmission and reception of signals.Enabling IBFD systems requires a substantial mitigation of a transmitter(Tx)’s strong self-interference(SI)signal into the receiver(Rx)channel.However,current state-ofthe-art approaches to tackle this challenge are inefficient in terms of performance,cost,and complexity,hindering the commercialization of IBFD techniques.In this work,we devise and demonstrate an innovative approach to realize IBFD systems that exhibit superior performance with a low-cost and lesscomplex architecture in an all-passive module.Our scheme is based on meticulously combining polarization-division multiplexing(PDM)with ferromagnetic nonreciprocity to achieve ultra-high isolation between Tx and Rx channels.Such an unprecedented conception has become feasible thanks to a concurrent dual-mode circulator—a new component introduced for the first time—as a key feature of our module,and a dual-mode waveguide that transforms two orthogonally polarized waves into two orthogonal waveguide modes.In addition,we propose a unique passive tunable secondary SI cancellation(SIC)mechanism,which is embedded within the proposed module and boosts the isolation over a relatively broad bandwidth.We report,solely in the analog domain,experimental isolation levels of 50,70,and 80 dB over 340,101,and 33 MHz bandwidth at the center frequency of interest,respectively,with excellent tuning capability.Furthermore,the module is tested in two real IBFD scenarios to assess its performance in connection with Tx-to-Rx leakage and modulation error in the presence of a Tx’s strong interference signal. 展开更多
关键词 In-band full-duplex transceiver 6G Polarization-division multiplexing Dual-mode nonreciprocity
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Particle-Based Iterative Blind Receiver for Orthogonal Frequency-Division Multiplexing with Frequency Offset 被引量:2
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作者 苏泳涛 张贤达 +1 位作者 高秋彬 丁子哲 《Tsinghua Science and Technology》 SCIE EI CAS 2007年第6期678-683,共6页
Orthogonal frequency-division multiplexing (OFDM) systems are sensitive to carrier frequency offset (CFO) which introduces intercarder interference and significantly degrades system performance. This paper describ... Orthogonal frequency-division multiplexing (OFDM) systems are sensitive to carrier frequency offset (CFO) which introduces intercarder interference and significantly degrades system performance. This paper describes an iterative blind receiver consisting of a sequential Monte Carlo detector, a CFO estimator, and a compensator to reduce intercarrier interference. The framework is of low complexity due to the separation of tasks in a joint detection problem. In addition, the CFO estimator utilizes soft output of the sequential Monte Carlo detector, which reduces the information loss caused by hard decisions and can obtain the CFO estimate in only one OFDM symbol. Simulation results demonstrate the effectiveness of the algorithm. 展开更多
关键词 sequential Monte Carlo (SMC) blind receiver orthogonal frequency division multiplexing (OFDM) carrier frequency offset (CFO)
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1.2 Gbit/s visible light transmission based on orthogonal frequency-division multiplexing using a phosphorescent white light-emitting diode and a pre-equalization circuit 被引量:4
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作者 黄星星 陈思源 +2 位作者 王智鑫 王一光 迟楠 《Chinese Optics Letters》 SCIE EI CAS CSCD 2015年第10期26-29,共4页
We propose a two-cascaded, constant-resistance, symmetrical bridged-T amplitude equalizer for a high-speed visible light communication(VLC) system. With the pre-equalization circuit, the-3 d B bandwidth of the VLC s... We propose a two-cascaded, constant-resistance, symmetrical bridged-T amplitude equalizer for a high-speed visible light communication(VLC) system. With the pre-equalization circuit, the-3 d B bandwidth of the VLC system can be extended from 12 to 235 MHz using a commercially available phosphorescent white light-emitting diode(LED), a blue filter, and a low-cost PIN photodiode. The data rate is 1.20 Gbit/s, exploiting 16-quadrature amplitude modulation-orthogonal frequency-division multiplexing with a 300 MHz modulation bandwidth over50 cm of free-space transmission under the pre-forward error correction limit of 3.8 × 10^-3. To our knowledge,this is the highest-3 d B bandwidth and the highest data rate ever achieved by using a pre-equalization circuit and white LED in a VLC system. 展开更多
关键词 equalization multiplexing visible bandwidth symmetrical quadrature cascaded correction illumination commercially
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Flat multifunctional liquid crystal elements through multi-dimensional information multiplexing 被引量:10
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作者 Dongliang Tang Zhenglong Shao +4 位作者 Xin Xie Yingjie Zhou Xiaohu Zhang Fan Fan Shuangchun Wen 《Opto-Electronic Advances》 SCIE EI CAS CSCD 2023年第4期39-47,共9页
Flat optical elements have attracted enormous attentions and act as promising candidates for the next generation of optical components.As one of the most outstanding representatives,liquid crystal(LC)has been widely a... Flat optical elements have attracted enormous attentions and act as promising candidates for the next generation of optical components.As one of the most outstanding representatives,liquid crystal(LC)has been widely applied in flat panel display industries and inspires the wavefront modulation with the development of LC alignment techniques.However,most LC elements perform only one type of optical manipulation and are difficult to realize the multifunctionality and light integration.Here,flat multifunctional liquid crystal elements(FMLCEs),merely composed of anisotropic LC molecules with space-variant orientations,are presented for multichannel information manipulation by means of polarization,space and wavelength multiplexing.Specifically,benefiting from the unique light response with the change of the incident polarization,observation plane,and working wavelength,a series of FMLCEs are demonstrated to achieve distinct near-and far-field display functions.The proposed strategy takes full advantage of basic optical parameters as the decrypted keys to improve the information capacity and security,and we expect it to find potential applications in information encryption,optical anti-counterfeiting,virtual/augmented reality,etc. 展开更多
关键词 MULTIFUNCTIONAL liquid crystal HOLOGRAPHY information multiplexing
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Time-sequential color code division multiplexing holographic display with metasurface 被引量:9
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作者 Xin Li Qinmiao Chen +4 位作者 Xue Zhang Ruizhe Zhao Shumin Xiao Yongtian Wang Lingling Huang 《Opto-Electronic Advances》 SCIE EI CAS CSCD 2023年第8期8-16,共9页
Color metasurface holograms are powerful and versatile platforms for modulating the amplitude,phase,polarization,and other properties of light at multiple operating wavelengths.However,the current color metasurface ho... Color metasurface holograms are powerful and versatile platforms for modulating the amplitude,phase,polarization,and other properties of light at multiple operating wavelengths.However,the current color metasurface holography can only realize static manipulation.In this study,we propose and demonstrate a multiplexing metasurface technique combined with multiwavelength code-division multiplexing(CDM)to realize dynamic manipulation.Multicolor code references are utilized to record information within a single metasurface and increase the information capacity and security for anticracks.A total of 48 monochrome images consisting of pure color characters and multilevel color video frames were reconstructed in dual polarization channels of the birefringent metasurface to exhibit high information density,and a video was displayed via sequential illumination of the corresponding code patterns to verify the ability of dynamic manipulation.Our approach demonstrates significant application potential in optical data storage,optical encryption,multiwavelengthversatile diffractive optical elements,and stimulated emission depletion microscopy. 展开更多
关键词 metasurface color holography dynamic display code division multiplexing
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Planar peristrophic multiplexing metasurfaces 被引量:4
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作者 Jia Chen Dapeng Wang +3 位作者 Guangyuan Si Siew Lang Teo Qian Wang Jiao Lin 《Opto-Electronic Advances》 SCIE EI CAS CSCD 2023年第8期40-48,共9页
As a promising counterpart of two-dimensional metamaterials,metasurfaces enable to arbitrarily control the wavefront of light at subwavelength scale and hold promise for planar holography and applicable multiplexing d... As a promising counterpart of two-dimensional metamaterials,metasurfaces enable to arbitrarily control the wavefront of light at subwavelength scale and hold promise for planar holography and applicable multiplexing devices.Nevertheless,the degrees of freedom(DoF)to orthogonally multiplex data have been almost exhausted.Compared with state-of-theart methods that extensively employ the orthogonal basis such as wavelength,polarization or orbital angular momentum,we propose an unprecedented method of peristrophic multiplexing by combining the spatial frequency orthogonality with the subwavelength detour phase principle.The orthogonal relationship between the spatial frequency of incident light and the locally shifted building blocks of metasurfaces can be regarded as an additional DoF.We experimentally demonstrate the viability of the multiplexed holograms.Moreover,this newly-explored orthogonality is compatible with conventional DoFs.Our findings will contribute to the development of multiplexing metasurfaces and provide a novel solution to nanophotonics,such as large-capacity chip-scale devices and highly integrated communication. 展开更多
关键词 peristrophic multiplexing metasurface HOLOGRAPHY
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Performance of phase-matching quantum key distribution based on wavelength division multiplexing technology 被引量:1
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作者 马海强 韩雁鑫 +1 位作者 窦天琦 李鹏云 《Chinese Physics B》 SCIE EI CAS CSCD 2023年第2期103-107,共5页
Quantum key distribution(QKD) generates information-theoretical secure keys between two parties based on the physical laws of quantum mechanics. The phase-matching(PM) QKD protocol allows the key rate to break the qua... Quantum key distribution(QKD) generates information-theoretical secure keys between two parties based on the physical laws of quantum mechanics. The phase-matching(PM) QKD protocol allows the key rate to break the quantum channel secret key capacity limit without quantum repeaters, and the security of the protocol is demonstrated by using equivalent entanglement. In this paper, the wavelength division multiplexing(WDM) technique is applied to the PM-QKD protocol considering the effect of crosstalk noise on the secret key rate. The performance of PM-QKD protocol based on WDM with the influence of adjacent classical channels and Raman scattering is analyzed by numerical simulations to maximize the total secret key rate of the QKD, providing a reference for future implementations of QKD based on WDM techniques. 展开更多
关键词 quantum key distribution wavelength division multiplexing secret key rate
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Research on Weighted Directed Dynamic Multiplexing Network of World Grain Trade Based on Improved MLP Framework
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作者 Shanyan Zhu Shicai Gong 《Journal of Computer and Communications》 2023年第7期191-207,共17页
As the main food source for humans, the global movement of the three major grains significantly impacts human survival and development. To investigate the evolution of the world cereal trade network and its developmen... As the main food source for humans, the global movement of the three major grains significantly impacts human survival and development. To investigate the evolution of the world cereal trade network and its development trend, a weighted directed dynamic multiplexed network was established using historical data on cereal trade, cereal import dependency ratio, and arable land per capita. Inspired by the MLP framework, we redefined the weight determination method for computing layer weights and edge weights of the target layer, modified the CN, RA, AA, and PA indicators, and proposed the node similarity indicator for weighted directed networks. The AUC metric, which measures the accuracy of the algorithm, has also been improved in order to finally obtain the link prediction results for the grain trading network. The prediction results were processed, such as web-based presentation and community partition. It was found that the number of generalized trade agreements does not have a decisive impact on inter-country cereal trade. The former large grain exporters continue to play an important role in this trade network. In the future, the world trade in cereals will develop in the direction of more frequent intercontinental trade and gradually weaken the intracontinental cereal trade. 展开更多
关键词 MLP Framework Food Security Dynamic multiplexed Networks Trade Network Link Forecasting
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Real-Time 4-Mode MDM Transmission Using Commercial 400G OTN Transceivers and All-Fiber Mode Multiplexers 被引量:1
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作者 REN Fang LI Yidan +2 位作者 YE Bing LIU Jianguo CHEN Weizhang 《ZTE Communications》 2024年第1期106-110,共5页
Weakly-coupled mode division multiplexing(MDM)technique is considered a promising candidate to enhance the capacity of an optical transmission system,in which mode multiplexers/demultiplexers(MMUX/MDEMUX)with low inse... Weakly-coupled mode division multiplexing(MDM)technique is considered a promising candidate to enhance the capacity of an optical transmission system,in which mode multiplexers/demultiplexers(MMUX/MDEMUX)with low insertion loss and modal crosstalk are the key components.In this paper,a low-modal-crosstalk 4-mode MMUX/MDEMUX for the weakly-coupled triple-ring-core few-mode fiber(TRC-FMF)is designed and fabricated with side-polishing processing.The measurement results show that a pair of MMUX/MDEMUX and 25 km weakly-coupled TRC-FMF MDM link achieve low modal crosstalk of lower than−17.5 dB and insertion loss of lower than 11.56 dB for all the four modes.Based on the TRC-FMF and all-fiber MMUX/MDEMUX,an experiment for 25 km real-time 4-mode 3-λwavelength division multiplexing(WDM)-MDM transmission is conducted using commercial 400G optical transport network(OTN)transceivers.The experimental results prove weakly-coupled MDM techniques facilitate a smooth upgrade of the optical transmission system. 展开更多
关键词 optical fiber couplers mode(de)multiplexers mode division multiplexing transmission
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Frequency-modulated continuous-wave multiplexed gas sensingbased on optical frequency comb calibration
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作者 Linhua Jia Xinghua Qu Fumin Zhang 《Chinese Physics B》 SCIE EI CAS CSCD 2024年第9期152-163,共12页
Laser absorption spectroscopy has proven to be an effective approach for gas sensing, which plays an important rolein the fields of military, industry, medicine and basic research. This paper presents a multiplexed ga... Laser absorption spectroscopy has proven to be an effective approach for gas sensing, which plays an important rolein the fields of military, industry, medicine and basic research. This paper presents a multiplexed gas sensing system basedon optical frequency comb (OFC) calibrated frequency-modulated continuous-wave (FMCW) tuning nonlinearity. Thesystem can be used for multi-parameter synchronous measurement of gas absorption spectrum and multiplexed opticalpath. Multi-channel parallel detection is realized by combining wavelength division multiplexing (WDM) and frequencydivision multiplexing (FDM) techniques. By introducing nonlinear optical crystals, broadband spectrum detection is simultaneouslyachieved over a bandwidth of hundreds of nanometers. An OFC with ultra-high frequency stability is used asthe frequency calibration source, which guarantees the measurement accuracy. The test samples involve H13C14N, C_(2)H_(2)and Rb vapor cells of varying densities and 5 parallel measurement experiments are designed. The results show that themeasurement accuracies of spectral absorption line and the optical path are 150 MHz and 20 m, respectively. The schemeoffers the advantages of multiplexed, multi-parameter, wide spectrum and high resolution detection, which can realize theidentification of multi-gas components and the high-precision inversion of absorption lines under different environments.The proposed sensor demonstrates great potential in the field of high-resolution absorption spectrum measurement for gassensing applications. 展开更多
关键词 frequency-modulated CONTINUOUS-WAVE (FMCW) technology optical FREQUENCY COMB multiplexing absorption spectroscopy
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A Study of Radiation-Induced Telomere Instability Using Multiplex Ligation-Dependent Probe Amplification (MLPA)
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作者 Alan Chant Ahmad Chaudary Christina M. Kraemer-Chant 《Advances in Biological Chemistry》 CAS 2024年第3期73-85,共13页
The integrity of the chromosomes for two WIL2-derived lymphoblastoid cell lines (TK6 and WTK1) in the presence and absence of ionizing radiation was analyzed by Multiplex Ligation-Dependent Probe Amplification (MLPA).... The integrity of the chromosomes for two WIL2-derived lymphoblastoid cell lines (TK6 and WTK1) in the presence and absence of ionizing radiation was analyzed by Multiplex Ligation-Dependent Probe Amplification (MLPA). The TK6 cell line has the native p53 tumor-suppressor gene, whereas WTK1 cells contain a p53 mutation. Each cell line was isolated pre- and post-irradiation (2 and 3 Gy) and analyzed by MLPA. The impact of irradiation on these two cell lines was investigated using probes that target specific regions on chromosomes associated with subtelomeric regions. Results indicate that WTK1 and TK6 are impacted differently after irradiation, and that each cell line presents its own unique MLPA profile. The most notable differences are the appearance of a number of probes in the post-irradiated MLPA profile that are not present in the controls, and two unique probe signals only seen in WTK1 cells. These results build on our previous studies that indicate how different human cell lines can be affected by radiation in significantly different ways depending on the presence or absence of wild type p53. 展开更多
关键词 Ionizing Radiation multiplex Ligation-Dependent Probe Amplification (MLPA) p53 Tumor Suppressor Telomere Instability
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Study on Distribution of Four Pseudomonas Species in Living Environment Using Multiplex PCR
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作者 Sachiyo Hayashi Koji Umezawa +12 位作者 Osamu Tsuzukibashi Akira Fukatsu Mana Fuchigami Chiaki Komine Hiroshi Yamamoto Mio Hagiwara-Hamano Yukiko Iizuka Satoshi Uchibori Masanobu Wakami Hiroshi Murakami Taira Kobayashi Masahiko Fukumoto Takato Nomoto 《Open Journal of Stomatology》 2024年第2期77-86,共10页
Purpose: The genus Pseudomonas is a ubiquitous microorganism frequently detected from immunocompromised patients. The inherent resistance to numerous antimicrobial agents contributes to the opportunistic character of ... Purpose: The genus Pseudomonas is a ubiquitous microorganism frequently detected from immunocompromised patients. The inherent resistance to numerous antimicrobial agents contributes to the opportunistic character of this pathogen exhaustive monitoring of this pathogen is considered of critical importance to public health organizations. The reliable identification method able to distinguish genetic close Pseudomonas species is needed, because these organisms are difficult to differentiate by phenotypic or biochemical methods. The purpose of the present study was to design species-specific primers in order to identify and detect four Pseudomonas species which are frequently detected from the human oral cavities, and to investigate the distribution of these organisms in the living environment using a multiplex PCR. Methods: Polymerase chain reaction (PCR) primers were designed based on partial sequences of the rpoD gene of four Pseudomonas species. Swab samples were collected from fifty washstands, and the distribution of Pseudomonas species was investigated using a conventional PCR at genus level and a multiplex PCR at species level. Results: Multiplex PCR method developed in this study was able to distinguish four Pseudomonas species clearly. The genus Pseudomonas was detected from all samples (100%), whereas P. putida, P, aeruginosa, P. stutzeri and P. fluorescens were detected at 44%, 8%, 4% and 2% in fifty swab samples, respectively. Conclusion: Our developed one-step multiplex PCR method is accurate, specific, cost-effective, time-saving, and works without requiring DNA extraction. It was indicated that washstands were the uninhabitable environment for P. putida, P, aeruginosa, P. stutzeri and P. fluorescens. 展开更多
关键词 Genus Pseudomonas multiplex PCR Living Environment
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A simple and efficient CRISPR/Cas9 system permits ultra-multiplex genome editing in plants
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作者 Suting Wu Htin Kyaw +11 位作者 Zhijun Tong Yirong Yang Zhiwei Wang Liying Zhang Lihua Deng Zhiguo Zhang Bingguang Xiao William Paul Quick Tiegang Lu Guoying Xiao Guannan Qin Xue'an Cui 《The Crop Journal》 SCIE CSCD 2024年第2期569-582,共14页
The development and maturation of the CRISPR/Cas genome editing system provides a valuable tool for plant functional genomics and genetic improvement.Currently available genome-editing tools have a limited number of t... The development and maturation of the CRISPR/Cas genome editing system provides a valuable tool for plant functional genomics and genetic improvement.Currently available genome-editing tools have a limited number of targets,restricting their application in genetic research.In this study,we developed a novel CRISPR/Cas9 plant ultra-multiplex genome editing system consisting of two template vectors,eight donor vectors,four destination vectors,and one primer-design software package.By combining the advantages of Golden Gate cloning to assemble multiple repetitive fragments and Gateway recombination to assemble large fragments and by changing the structure of the amplicons used to assemble sg RNA expression cassettes,the plant ultra-multiplex genome editing system can assemble a single binary vector targeting more than 40 genomic loci.A rice knockout vector containing 49 sg RNA expression cassettes was assembled and a high co-editing efficiency was observed.This plant ultra-multiplex genome editing system advances synthetic biology and plant genetic engineering. 展开更多
关键词 CRISPR/Cas9 multiplex genome editing Assembly system PLANT
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Establishment and performance analysis of a new multiplex detection method for influenza an and B virus antigen
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作者 Cheng-Jing Xia Bao-Hua Li +3 位作者 Yan-Ni Guo Xiao-He Zhou Run-Ling Zhang Ying-No Niu 《World Journal of Clinical Cases》 SCIE 2024年第23期5338-5345,共8页
BACKGROUND Influenza A and B virus detection is pivotal in epidemiological surveillance and disease management.Rapid and accurate diagnostic techniques are crucial for timely clinical intervention and outbreak prevent... BACKGROUND Influenza A and B virus detection is pivotal in epidemiological surveillance and disease management.Rapid and accurate diagnostic techniques are crucial for timely clinical intervention and outbreak prevention.Quantum dot-encoded microspheres have been widely used in immunodetection.The integration of quantum dot-encoded microspheres with flow cytometry is a well-established technique that enables rapid analysis.Thus,establishing a multiplex detection method for influenza A and B virus antigens based on flow cytometry quantum dot microspheres will help in disease diagnosis.AIM To establish a codetection method of influenza A and B virus antigens based on flow cytometry quantum dot-encoded microsphere technology,which forms the foundation for the assays of multiple respiratory virus biomarkers.METHODS Different quantum dot-encoded microspheres were used to couple the monoclonal antibodies against influenza A and B.The known influenza A and B antigens were detected both separately and simultaneously on a flow cytometer,and the detection conditions were optimized to establish the influenza A and B antigen codetection method,which was utilized for their detection in clinical samples.The results were compared with the fluorescence quantitative polymerase chain reaction(PCR)method to validate the clinical performance of this method.RESULTS The limits of detection of this method were 26.1 and 10.7 pg/mL for influenza A and B antigens,respectively,which both ranged from 15.6 to 250000 pg/mL.In the clinical sample evaluation,the proposed method well correlated with the fluorescent quantitative PCR method,with positive,negative,and overall compliance rates of 57.4%,100%,and 71.6%,respectively.CONCLUSION A multiplex assay for quantitative detection of influenza A and B virus antigens has been established,which is characterized by high sensitivity,good specificity,and a wide detection range and is promising for clinical applications. 展开更多
关键词 Influenza A Influenza B Quantum dot microspheres Antigen detection multiplex detection
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