This paper aims to explore comprehensive utilization way of discarded fresh tobacco leaf. Based on modern tobacco demands on green,environmental protection and sustainable development,as well as actual situation of bi...This paper aims to explore comprehensive utilization way of discarded fresh tobacco leaf. Based on modern tobacco demands on green,environmental protection and sustainable development,as well as actual situation of biogas production facility in some villages of local tobacco area,this paper selects Longwan Village of Fengshiyan Town as the experimental site and explores the rational way of discarded fresh tobacco leaf biogas fermentation. Results show that cutting and fermentation of discarded fresh tobacco leaf before adding into biogas digester is conductive to improving p H in fermentation process,reducing crust amount,and effectively improving biogas production amount and rate. The study can provide reference for effective processing and rational utilization of discarded fresh tobacco leaf.展开更多
The importance of controlled temperature during the four-days co-cultivation period was evaluated under the most physiologically relevant conditions for Agrobacterium tumefaciens-mediated transformation of tobacco (Ni...The importance of controlled temperature during the four-days co-cultivation period was evaluated under the most physiologically relevant conditions for Agrobacterium tumefaciens-mediated transformation of tobacco (Nicotiana tabacum L. cv. Xanthi (nn, Smith)) leaf disks. We compared the effect of temperatures ranging from 15°C, 18°C, 20°C, 22°C to 25°C on the stable expression of β-glucuronidase (GUS) activity of 14 days old hygromycin-selected leaf disks, and on the increase in the fresh weight yield of 28 days old kanamycin-selected calli. The highest average of GUS activity was obtained at 20°C among the five temperatures tested although the difference between the 18°C and 20°C treatment was not statistically significant. The GUS activity at 15°C was statistically lower than those at 18°C and 20°C. The GUS activity in 22°C treatment was an intermediate between the highest (18/20°C) and second highest averages (15°C), and was not statistically significantly different. The lowest average of GUS activity was observed at 25°C. The highest increase in the plate average of fresh weight yield was obtained at 20°C among the five temperature tested. The 20°C treatment was statistically significantly better than the 15°C and 18°C treatments. The 20°C co-cultivation treatment resulted in the higher FW yield than 22°C and 25°C even though the differences were not statistically significant. In conclusion, low co-cultivation temperature at 20°C resulted in the reproducible maximum increase in both the fresh weight yield and stable expression of GUS activity after transformation of tobacco leaf disks.展开更多
为探索地域及部位对烤烟成熟鲜烟叶化学成分的影响,采用红花大金元品种在昆明烟区海拔1920和1650 m 2个试点对3个部位成熟鲜烟叶的10种化学成分进行同步研究。结果表明,①总糖、还原糖含量在叶片中自下而上的增减趋势与海拔关系密切,高...为探索地域及部位对烤烟成熟鲜烟叶化学成分的影响,采用红花大金元品种在昆明烟区海拔1920和1650 m 2个试点对3个部位成熟鲜烟叶的10种化学成分进行同步研究。结果表明,①总糖、还原糖含量在叶片中自下而上的增减趋势与海拔关系密切,高海拔点呈递减趋势,低海拔点呈递增趋势,而其他8种成分的变化趋势2个点基本一致,主要受叶片生长部位控制。②成熟鲜烟叶蛋白质、总多酚和总氮含量在叶片中自下而上呈递增趋势,淀粉含量呈"抛物线"变化,下部叶和中部叶两地含量差异较小,仅上部叶含量差异较大。③成熟鲜烟叶石油醚提取物、烟碱、挥发碱和挥发酸含量均随部位的升高而递增,两地间的含量差异是上部叶最大,中部叶次之,下部叶最小。展开更多
文摘This paper aims to explore comprehensive utilization way of discarded fresh tobacco leaf. Based on modern tobacco demands on green,environmental protection and sustainable development,as well as actual situation of biogas production facility in some villages of local tobacco area,this paper selects Longwan Village of Fengshiyan Town as the experimental site and explores the rational way of discarded fresh tobacco leaf biogas fermentation. Results show that cutting and fermentation of discarded fresh tobacco leaf before adding into biogas digester is conductive to improving p H in fermentation process,reducing crust amount,and effectively improving biogas production amount and rate. The study can provide reference for effective processing and rational utilization of discarded fresh tobacco leaf.
文摘The importance of controlled temperature during the four-days co-cultivation period was evaluated under the most physiologically relevant conditions for Agrobacterium tumefaciens-mediated transformation of tobacco (Nicotiana tabacum L. cv. Xanthi (nn, Smith)) leaf disks. We compared the effect of temperatures ranging from 15°C, 18°C, 20°C, 22°C to 25°C on the stable expression of β-glucuronidase (GUS) activity of 14 days old hygromycin-selected leaf disks, and on the increase in the fresh weight yield of 28 days old kanamycin-selected calli. The highest average of GUS activity was obtained at 20°C among the five temperatures tested although the difference between the 18°C and 20°C treatment was not statistically significant. The GUS activity at 15°C was statistically lower than those at 18°C and 20°C. The GUS activity in 22°C treatment was an intermediate between the highest (18/20°C) and second highest averages (15°C), and was not statistically significantly different. The lowest average of GUS activity was observed at 25°C. The highest increase in the plate average of fresh weight yield was obtained at 20°C among the five temperature tested. The 20°C treatment was statistically significantly better than the 15°C and 18°C treatments. The 20°C co-cultivation treatment resulted in the higher FW yield than 22°C and 25°C even though the differences were not statistically significant. In conclusion, low co-cultivation temperature at 20°C resulted in the reproducible maximum increase in both the fresh weight yield and stable expression of GUS activity after transformation of tobacco leaf disks.
文摘为探索地域及部位对烤烟成熟鲜烟叶化学成分的影响,采用红花大金元品种在昆明烟区海拔1920和1650 m 2个试点对3个部位成熟鲜烟叶的10种化学成分进行同步研究。结果表明,①总糖、还原糖含量在叶片中自下而上的增减趋势与海拔关系密切,高海拔点呈递减趋势,低海拔点呈递增趋势,而其他8种成分的变化趋势2个点基本一致,主要受叶片生长部位控制。②成熟鲜烟叶蛋白质、总多酚和总氮含量在叶片中自下而上呈递增趋势,淀粉含量呈"抛物线"变化,下部叶和中部叶两地含量差异较小,仅上部叶含量差异较大。③成熟鲜烟叶石油醚提取物、烟碱、挥发碱和挥发酸含量均随部位的升高而递增,两地间的含量差异是上部叶最大,中部叶次之,下部叶最小。
