Effects of Inoculation with Phosphate Solubilizing Bacteria on the Physiology,Biochemistry,and Expression of Genes Related to the Protective Enzyme System of Fritillaria taipaiensis P.Y.Li

Fritillaria taipaiensis P.Y.Li is a widely used medicinal herb in treating pulmonary diseases.In recent years,its wild resources have become scarce,and the demand for efficient artificial cultivation has significantly increased.This article is the first to apply phosphate solubilizing bacteria isolated from the rhizosphere soil of F.taipaiensis P.Y.Li to the cultivation process of F.taipaiensis P.Y.Li.The aim is to identify suitable reference strains for the artificial cultivation and industrial development of F.taipaiensis P.Y.Li by examining the effects of various phosphate solubilizing bacteria and their combinations on photosynthesis,physiological and biochemical properties,and gene expression related to the protective enzyme system in F.taipaiensis P.Y.Li.The experiment,conducted in pots at room temperature,included a control group(CK)and groups inoculated with inorganic phosphorussolubilizing bacteria:W1(Bacillus cereus),W2(Serratia plymuthica),W12(Bacillus cereus and Serratia plymuthica),and groups inoculated with organophosphorus-solubilizing bacteria:Y1(Bacillus cereus),Y2(Bacillus cereus),Y12(Bacillus cereus and Bacillus cereus),totaling seven groups.Compared to CK,most growth indices in the bacterial addition groups showed significant differences,with W12 achieving the highest values in all indices except the leaf area index.The content of photosynthetic pigments,photosynthetic parameters,and osmoregulatory substances increased variably in each bacterial treatment group.W12 exhibited the highest content of chlorophyll a and soluble protein,while W1 had the highest free proline content.The activities of peroxidase(POD),superoxide dismutase(SOD),and catalase(CAT)in all inoculated groups were higher than in CK,with significant changes in SOD and CAT activities.The malondialdehyde(MDA)content in all inoculated groups was lower than in CK,with Y12 being the lowest,at approximately 30%of CK.Gene expression corresponding to these three enzymes also increased variably,with POD expression in Y2 being the highest at 2.73 times that of CK.SOD and CAT expression in Y12 were the highest,at 1.84 and 4.39 times that of CK,respectively.These results indicate that inoculating phosphate solubilizing bacteria can enhance the growth of F.taipaiensis P.Y.Li,with the mixed inoculation groups W12 and Y12 demonstrating superior effects.This lays a theoretical foundation for selecting bacterial fertilizers in the cultivation process of F.taipaiensis P.Y.Li.

Analysis of the bioactive components from different growth stages of Fritillaria taipaiensis P. Y. Li

High-performance liquid chromatography(HPLC)coupled with an evaporative light scattering detector(ELSD)or a diode array detector(DAD)were utilized for the quantitative analysis of 4 alkaloids(peimisine,sipeimine,peimine and peiminine)and 9 nucleosides and nucleobases(uracil,uridine,adenosine,adenine,inosine,thymine,cytidine,guanosine and thymidine)from Fritillaria taipaiensis P.Y.Li that had been cultivated in the same field for 2–6 years.The content of peimisine,sipeimine,peimine,peiminine,uracil,thymine,adenine and inosine in plants cultivated for 2–4 years was significantly higher than that of plants cultivated for 5–6 years,while the content of cytidine,uridine,guanosine,thymidine and adenosine did not change over this period.This is the first evaluation of variation in the bioactive compounds in F.taipaiensis over its life cycle.

太白贝母生物碱的HPLC指纹图谱研究

目的:建立太白贝母的HPLC指纹图谱,为评价其质量提供有效的参考方法。方法:采用HPLC-ELSD检测法,高效液相色谱柱为Agilent Extend-C18(4.0 mm×250 mm,5μm);以0.03%二乙胺水-甲醇为流动相梯度洗脱,柱温30℃;流速1 m L·min-1;进样量10μL。蒸发光散射检测器漂移管温度96℃;载气流速2.7 L·min-1。结果:14批太白贝母样品HPLC指纹图谱相似度都在82%以上,精密度、稳定性、重复性实验中各共有峰相对保留时间和相对峰面积的RSD均<4.5%,结果能较全面地反映太白贝母药材中主要成分的分布特征。结论:本研究可为太白贝母的质量控制研究提供参考。

太白贝母提取物对人结肠癌SW480细胞增殖抑制作用及其机制的研究

目的研究太白贝母总生物碱提取物对人结肠癌SW480细胞的增殖抑制作用及机制。方法太白贝母总生物碱提取物作用于人结肠癌SW480细胞,分为空白细胞组、0.25%DMSO溶剂处理组、太白贝母总生物碱提取物处理组(含总生物碱浓度分别为36.9 mg·L^(-1)、49.2 mg·L^(-1)、61.5 mg·L^(-1))。采用MTT试验、流式细胞术检测细胞周期,倒置光镜及透射电镜等观察各组细胞的增殖抑制情况、细胞周期变化、细胞显微和亚显微结构变化。结果与0.25%DMSO溶剂处理组比较,61.5 mg·L^(-1)总生物碱提取物处理细胞24 h、48 h、72 h后,细胞生长抑制呈现较为明显的时间依赖性;含太白贝母总生物碱36.9 mg·L^(-1)、49.2 mg·L^(-1)、61.5 mg·L^(-1)的提取物分别处理细胞48 h,细胞生长抑制呈现显著的剂量依赖性;流式细胞术PI单染结果显示,36.9 mg·L^(-1)、49.2 mg·L^(-1)太白贝母总生物碱提取物可将细胞阻滞于G2和S期,61.5 mg·L^(-1)太白贝母总生物碱提取物可将细胞阻滞于G2和G1期;61.5 mg·L^(-1)太白贝母总生物碱提取物处理48 h,细胞显微和亚显微结构发生显著坏死样变化。结论碱化热回流法提取的太白贝母总生物碱提取物具有明显的抗结肠癌SW480细胞活性,阻滞细胞周期进程为其机制之一。

川贝母新资源太白贝母的研究进展

对太白贝母的药用历史、分布与生境、栽培与组织培养、化学成分、药理作用等方面的研究进行了综述,提出太白贝母的研究方向。

太白贝母生产数值区划研究

本文在太白贝母的应用历史及现状调查的基础上,对其生态习性进行了文献调研和实地考察,以太白贝母分布区55个样点为分析基点,选取≥10℃积温、年平均气温、1月平均气温、1月最低气温、7月平均气温、7月最高气温、相对湿度、年均日照时间、年降水量、土壤类型等影响太白贝母生长发育的关键生态因子,再应用《中药材产地适宜性分析地理信息系统》(TCMGIS-Ⅱ)对太白贝母(Fritillaria taipaiensis P.Y.Li)在全国的生态适宜区进行分析。研究结果表明,适宜产地主要分布于陕西、湖北、甘肃、重庆、四川、云南等省区。研究结果有助于太白贝母GAP规范化种植基地选择及川贝母资源的可持续利用。

五峰尖贝原植物形态及有效成分研究

通过对湖北民间常用中药——五峰尖贝的产地调查,系统地对其原植物及药用器官(鳞茎)的形态及有效成分进行了研究。确定五峰尖贝为百合科(Liliaceae)植物太白贝母(Fritilliaria taipaiensis P.V.Li)。