Yinzhou District of Ningbo City is the home and main production area of Fritillaria thunbergii Miq. Based on the analysis of Fritillaria thunbergii Miq. production in Yinzhou District in 2015,this paper firstly introd...Yinzhou District of Ningbo City is the home and main production area of Fritillaria thunbergii Miq. Based on the analysis of Fritillaria thunbergii Miq. production in Yinzhou District in 2015,this paper firstly introduced current situation of Fritillaria thunbergii Miq. planting. The competition of Fritillaria thunbergii Miq. products was in disorder,the market price in 2015 dropped to half in 2014,and many Fritillaria thunbergii Miq. growers suffered losses. To keep sustainable development of traditional Fritillaria thunbergii Miq. industry in old production areas and maintain brand and name of genuine materials of " Zhejiang 8 Famous Kinds Herbal Medicines",it is required to actively reduce the inventory,make up short board,strengthen advantages,implement the reform of supply font,keep up with the times and market,and succeed through improving the quality.展开更多
Background:The dry bulb of Fritillaria thunbergii Miq.is a traditional Chinese medicine,and is the leading product of the geo-authentic crude drugs‘Zhebawei’in Zhejiang Province.Fritillaria thunbergii requires more ...Background:The dry bulb of Fritillaria thunbergii Miq.is a traditional Chinese medicine,and is the leading product of the geo-authentic crude drugs‘Zhebawei’in Zhejiang Province.Fritillaria thunbergii requires more potassium(K)than nitrogen and phosphorus,while the response of F.thunbergii to K has rarely been studied.Objective and Methods:To evaluate the K nutritional status of F.thunbergii,a 2-year field experiment was conducted under six K application rates(0,40,80,120,160,and 200 kg K_(2)O ha−1)with two F.thunbergii cultivars(ZB1,narrow-leaf;ZB2,broad-leaf).Results:The bulbus Fritillariae Thunbergii(BFT)yield increased to a plateau with more than~120 kg K_(2)O ha-1.The BFT quality met the standard of Pharmacopoeia of P.R.China with more than 40.0 kg K_(2)O ha^(-1).Leaf K concentration was significantly correlated with BFT yield,and was more sensitive to K application rate as compared to those of stems and flowers.Potassium application extended the duration of rapid leaf-biomass accumulation and increased the accumulation rate.Two cultivars had similar response patterns to K application,but ZB2 had higher K partial productivity,leaf-biomass,and leaf K concentration than ZB1.Conclusion:108.4-128.0 kg K_(2)O ha^(-1) was the optimal K application range for BFT yield.The potassium nutrition index(KNI)model established based on leaf K concentration has biological significance and can be used for dynamic diagnosis of K nutritional status of F.thunbergii with both accuracy and simplicity.展开更多
The bulbs of Fritillaria spp.(Liliaceae)are used in Chinese medicine as antipyretic,anti-tussive,expectorant,etc.In order to search for the bioactive constituents from this genus,thebulbs of four species of medicinal ...The bulbs of Fritillaria spp.(Liliaceae)are used in Chinese medicine as antipyretic,anti-tussive,expectorant,etc.In order to search for the bioactive constituents from this genus,thebulbs of four species of medicinal Fritillaria plants have been investigated.Thirteen compoundswere isolated from Fritillaria thunbergii Miq.,three of them were new compounds.Fourteencompounds from Fritillaria thunbergii var.chekiangensis Hsiao et K.C.Hsia collected inDongyang of Zhejiang Province,four of them are new compounds and another one was first iso-展开更多
为区分浙贝母外观品质等级,本研究利用数字电子眼系统及图像标注工具构建浙贝母数据集,选择若干统计学习算法和目标检测算法在该数据集上进行训练与测试。结果表明:目标检测算法YOLO(you only look once)系列YOLO-X所得模型的效果最佳...为区分浙贝母外观品质等级,本研究利用数字电子眼系统及图像标注工具构建浙贝母数据集,选择若干统计学习算法和目标检测算法在该数据集上进行训练与测试。结果表明:目标检测算法YOLO(you only look once)系列YOLO-X所得模型的效果最佳。为优化YOLO-X,根据浙贝母数据集的特点,针对性地向YOLO-X的主干特征提取网络末端嵌入空洞卷积结构,以加强模型对尺度特征的敏感度。改进后模型(空洞率为4)的平均精确率均值为99.01%,对于特级、一级、二级、虫蛀、霉变、破碎浙贝母的平均精确率分别为99.97%、98.33%、98.47%、98.71%、99.73%、98.85%,精确率和召回率的加权调和平均数(F1)分别为0.99、0.92、0.94、0.97、0.99、0.97。本研究在不增加参数量、计算量或者对算法进行大规模改动的情况下,改善了模型的检测效果,为后续浙贝母检测平台的搭建提供了科学依据。展开更多
目的:基于网络药理学探讨浙贝母-夏枯草治疗甲状腺结节(thyroid nodules)的作用机制。方法:在中药系统药理数据库和分析平台(traditional Chinese medicine systems pharmacology database and analysis platform, TCMSP)检索浙贝母、...目的:基于网络药理学探讨浙贝母-夏枯草治疗甲状腺结节(thyroid nodules)的作用机制。方法:在中药系统药理数据库和分析平台(traditional Chinese medicine systems pharmacology database and analysis platform, TCMSP)检索浙贝母、夏枯草的化学成分,利用PharmMapper数据库获取化学成分靶点,并采用Cytoscape 3.7.1软件构建“药物-活性成分-靶点”网络。基于Genecards数据库、在线人类孟德尔遗传数据库、治疗靶点数据库获得甲状腺结节相关靶点。将有效成分相关靶点与疾病靶点导入韦恩图网站进行在线分析,获得交集靶点,并利用STRING数据库构建蛋白互作网络(protein-protein interaction networks, PPI)模型,利用Cytoscape 3.7.1软件构建PPI网络并筛选核心靶点。将药物及其活性成分、交集靶点及疾病导入Cytoscape 3.7.1软件绘制成“药物-活性成分-交集靶点-疾病”网络图。利用Metascape数据库对交集靶点进行基因本体(gene ontology, GO)功能分析和京都基因与基因组百科全书(kyoto encyclopedia of genes and genomes, KEGG)信号通路富集分析。采用AutoDock软件对关键靶点与活性成分进行分子对接。结果:浙贝母-夏枯草药对共15个活性成分,171个相关靶点。甲状腺结节疾病靶点1 563个。经韦恩图网站在线分析后得到浙贝母-夏枯草治疗甲状腺结节相关的靶点108个。交集靶点PPI网络分析得到AKT1、肿瘤蛋白P53(tumor protein P53,TP53)、丝裂原活化蛋白激酶1(mitogen-activated protein kinase 1,MAPK1)为核心靶点。GO富集分析得到生物过程1 705条、分子功能136条、细胞组分115条。KEGG富集分析获得202个信号通路。分子对接结果表明,浙贝母-夏枯草主要有效活性成分与靶点受体蛋白可以较好的结合。结论:浙贝母-夏枯草药对可通过多成分、多靶点、多通路治疗甲状腺结节。展开更多
文摘Yinzhou District of Ningbo City is the home and main production area of Fritillaria thunbergii Miq. Based on the analysis of Fritillaria thunbergii Miq. production in Yinzhou District in 2015,this paper firstly introduced current situation of Fritillaria thunbergii Miq. planting. The competition of Fritillaria thunbergii Miq. products was in disorder,the market price in 2015 dropped to half in 2014,and many Fritillaria thunbergii Miq. growers suffered losses. To keep sustainable development of traditional Fritillaria thunbergii Miq. industry in old production areas and maintain brand and name of genuine materials of " Zhejiang 8 Famous Kinds Herbal Medicines",it is required to actively reduce the inventory,make up short board,strengthen advantages,implement the reform of supply font,keep up with the times and market,and succeed through improving the quality.
文摘Background:The dry bulb of Fritillaria thunbergii Miq.is a traditional Chinese medicine,and is the leading product of the geo-authentic crude drugs‘Zhebawei’in Zhejiang Province.Fritillaria thunbergii requires more potassium(K)than nitrogen and phosphorus,while the response of F.thunbergii to K has rarely been studied.Objective and Methods:To evaluate the K nutritional status of F.thunbergii,a 2-year field experiment was conducted under six K application rates(0,40,80,120,160,and 200 kg K_(2)O ha−1)with two F.thunbergii cultivars(ZB1,narrow-leaf;ZB2,broad-leaf).Results:The bulbus Fritillariae Thunbergii(BFT)yield increased to a plateau with more than~120 kg K_(2)O ha-1.The BFT quality met the standard of Pharmacopoeia of P.R.China with more than 40.0 kg K_(2)O ha^(-1).Leaf K concentration was significantly correlated with BFT yield,and was more sensitive to K application rate as compared to those of stems and flowers.Potassium application extended the duration of rapid leaf-biomass accumulation and increased the accumulation rate.Two cultivars had similar response patterns to K application,but ZB2 had higher K partial productivity,leaf-biomass,and leaf K concentration than ZB1.Conclusion:108.4-128.0 kg K_(2)O ha^(-1) was the optimal K application range for BFT yield.The potassium nutrition index(KNI)model established based on leaf K concentration has biological significance and can be used for dynamic diagnosis of K nutritional status of F.thunbergii with both accuracy and simplicity.
文摘The bulbs of Fritillaria spp.(Liliaceae)are used in Chinese medicine as antipyretic,anti-tussive,expectorant,etc.In order to search for the bioactive constituents from this genus,thebulbs of four species of medicinal Fritillaria plants have been investigated.Thirteen compoundswere isolated from Fritillaria thunbergii Miq.,three of them were new compounds.Fourteencompounds from Fritillaria thunbergii var.chekiangensis Hsiao et K.C.Hsia collected inDongyang of Zhejiang Province,four of them are new compounds and another one was first iso-
文摘为区分浙贝母外观品质等级,本研究利用数字电子眼系统及图像标注工具构建浙贝母数据集,选择若干统计学习算法和目标检测算法在该数据集上进行训练与测试。结果表明:目标检测算法YOLO(you only look once)系列YOLO-X所得模型的效果最佳。为优化YOLO-X,根据浙贝母数据集的特点,针对性地向YOLO-X的主干特征提取网络末端嵌入空洞卷积结构,以加强模型对尺度特征的敏感度。改进后模型(空洞率为4)的平均精确率均值为99.01%,对于特级、一级、二级、虫蛀、霉变、破碎浙贝母的平均精确率分别为99.97%、98.33%、98.47%、98.71%、99.73%、98.85%,精确率和召回率的加权调和平均数(F1)分别为0.99、0.92、0.94、0.97、0.99、0.97。本研究在不增加参数量、计算量或者对算法进行大规模改动的情况下,改善了模型的检测效果,为后续浙贝母检测平台的搭建提供了科学依据。
文摘目的:基于网络药理学探讨浙贝母-夏枯草治疗甲状腺结节(thyroid nodules)的作用机制。方法:在中药系统药理数据库和分析平台(traditional Chinese medicine systems pharmacology database and analysis platform, TCMSP)检索浙贝母、夏枯草的化学成分,利用PharmMapper数据库获取化学成分靶点,并采用Cytoscape 3.7.1软件构建“药物-活性成分-靶点”网络。基于Genecards数据库、在线人类孟德尔遗传数据库、治疗靶点数据库获得甲状腺结节相关靶点。将有效成分相关靶点与疾病靶点导入韦恩图网站进行在线分析,获得交集靶点,并利用STRING数据库构建蛋白互作网络(protein-protein interaction networks, PPI)模型,利用Cytoscape 3.7.1软件构建PPI网络并筛选核心靶点。将药物及其活性成分、交集靶点及疾病导入Cytoscape 3.7.1软件绘制成“药物-活性成分-交集靶点-疾病”网络图。利用Metascape数据库对交集靶点进行基因本体(gene ontology, GO)功能分析和京都基因与基因组百科全书(kyoto encyclopedia of genes and genomes, KEGG)信号通路富集分析。采用AutoDock软件对关键靶点与活性成分进行分子对接。结果:浙贝母-夏枯草药对共15个活性成分,171个相关靶点。甲状腺结节疾病靶点1 563个。经韦恩图网站在线分析后得到浙贝母-夏枯草治疗甲状腺结节相关的靶点108个。交集靶点PPI网络分析得到AKT1、肿瘤蛋白P53(tumor protein P53,TP53)、丝裂原活化蛋白激酶1(mitogen-activated protein kinase 1,MAPK1)为核心靶点。GO富集分析得到生物过程1 705条、分子功能136条、细胞组分115条。KEGG富集分析获得202个信号通路。分子对接结果表明,浙贝母-夏枯草主要有效活性成分与靶点受体蛋白可以较好的结合。结论:浙贝母-夏枯草药对可通过多成分、多靶点、多通路治疗甲状腺结节。
