A new compound, subglain B, was isolated from the stems of Uvaria tonkinensis var. subglabra and its structure was identified as 1S, 2R, 3S, 6R-1-benzoyloxymethylene-1,2- dihydroxy-3-benzoyloxy-6-chlorocyclohex-4-ene ...A new compound, subglain B, was isolated from the stems of Uvaria tonkinensis var. subglabra and its structure was identified as 1S, 2R, 3S, 6R-1-benzoyloxymethylene-1,2- dihydroxy-3-benzoyloxy-6-chlorocyclohex-4-ene (1), by spectral evidences.展开更多
Objective:To investigate the antitumor effect of maesopsin 4-O-β-glucoside(TAT2) isolated from the leaves of Artocarpus tonkinensis(A.tonkinensis) A.Chev.ex Gagnep.Methods:The antitumor activity of TAT2 was evaluated...Objective:To investigate the antitumor effect of maesopsin 4-O-β-glucoside(TAT2) isolated from the leaves of Artocarpus tonkinensis(A.tonkinensis) A.Chev.ex Gagnep.Methods:The antitumor activity of TAT2 was evaluated in Lewis lung carcinoma(LLC) tumor-bearing mice.BALB/c mice had tumors induced by implantation with 2× 10~6 LLC cells into the subcutaneous right posterior Hank.Tumor-bearing mice were treated orally with a range of doses of TAT2 and a standard drug,doxorubicin.Animals were observed for tumor growth and mortality rate.Blood was collected to determine hematological and biochemical parameters.Results TAT2 was isolated from an ethanolic extract of 1.tonkinensis leaves.Its structure was determined by MS and NMR spectroscopy,and identified as TAT2.The compound did not show acute toxicity at the highest dose tested(2 OIK) mg/kg body weight).TAT2 exhibited antitumor activity by decreasing tumor growth,increasing the survival rale,and ameliorating some hematological and biochemical parameters at doses of 100 and 200 mg/kg body weight(P<0.05).Conclusions:These results indicate that TAT2 possesses clear antitumor activity.Due to its bioavailability and low toxicity,and the fact that it could be isolated in a large scale from A.tonkinensis leaves,the compound shows promise as a potential anticancer drug.展开更多
[Objectives]To explore the response characteristics of chlorophyll and photosynthesis in Styrax tonkinensis under different concentrations of copper and cadmium stress and analyze the internal mechanism of the effects...[Objectives]To explore the response characteristics of chlorophyll and photosynthesis in Styrax tonkinensis under different concentrations of copper and cadmium stress and analyze the internal mechanism of the effects of copper and cadmium pollution stress on photosynthesis.[Methods]Taking seedlings of S.tonkinensis as the research object,the response characteristics of chlorophyll and photosynthesis of S.tonkinensis were compared under different concentrations of copper and cadmium.[Results]With the increase of copper and cadmium treatment concentrations,the chlorophyll content first rose and then fell.With the increase of cadmium concentration,the net photosynthetic rate of S.tonkinensis plants increased from decrease to increase,and the stomatal conductance,transpiration rate and intercellular CO_(2) concentration all increased.[Conclusions]Under the stress of different concentrations of cadmium,the photosynthetic characteristics of S.tonkinensis were not obviously inhibited.Copper stress has inhibitory effect on photosynthesis of S.tonkinensis.展开更多
目的基于网络药理学、动物实验及荧光定量PCR技术阐明山豆根改善急性咽炎的分子机制。方法利用中药系统药理学分析平台(Traditional Chinese Medicine systems pharmacology database and analysis platform,TCMSP)数据库收集山豆根的...目的基于网络药理学、动物实验及荧光定量PCR技术阐明山豆根改善急性咽炎的分子机制。方法利用中药系统药理学分析平台(Traditional Chinese Medicine systems pharmacology database and analysis platform,TCMSP)数据库收集山豆根的活性成分和作用靶点。通过GeneCards、OMIM、DrugBank和Disgenet数据库获取急性咽炎相关的靶点。筛选获得两者的共同靶标后,利用STRING数据库进行构建蛋白互作网络,采用Metascape平台进行通路分析。同时应用Cytoscape软件构建“中药-疾病-成分-靶点”网络和“中药-疾病-成分-靶点-通路”网络。建立大鼠急性咽炎模型,研究山豆根水提取物对大鼠急性咽炎的影响。利用荧光定量PCR技术研究山豆根对大鼠急性咽炎模型咽部组织关键通路上的关键基因靶点的影响。结果该实验获取山豆根活性成分21个的相关作用靶点509个,获取急性咽炎相关的靶点2167个,山豆根与急性咽炎的共同靶点194个。KEGG通路分析筛选了344条相关信号通路,其中显示IL-17信号通路、NF-kappa B信号通路和白细胞跨内皮迁移通路可能在山豆根改善急性咽炎的过程中起关键作用。动物实验表明,山豆根水提取物低剂量组对急性咽炎治疗效果较好。荧光定量PCR实验结果表明,山豆根低剂量组明显下调了白细胞跨内皮迁移通路ITGB2、PIK3CA、PIK3CD和PTPN11基因的表达水平(P<0.05)。结论山豆根改善急性咽炎具有多成分、多靶点、多通路协同作用的特点。展开更多
基金the National Natural Science Foundation of China(No.39970084)the Chinese Doctoral Grants of the Ministry of Science and Technology of China(No.96-901-96-54)for financial support
文摘A new compound, subglain B, was isolated from the stems of Uvaria tonkinensis var. subglabra and its structure was identified as 1S, 2R, 3S, 6R-1-benzoyloxymethylene-1,2- dihydroxy-3-benzoyloxy-6-chlorocyclohex-4-ene (1), by spectral evidences.
文摘Objective:To investigate the antitumor effect of maesopsin 4-O-β-glucoside(TAT2) isolated from the leaves of Artocarpus tonkinensis(A.tonkinensis) A.Chev.ex Gagnep.Methods:The antitumor activity of TAT2 was evaluated in Lewis lung carcinoma(LLC) tumor-bearing mice.BALB/c mice had tumors induced by implantation with 2× 10~6 LLC cells into the subcutaneous right posterior Hank.Tumor-bearing mice were treated orally with a range of doses of TAT2 and a standard drug,doxorubicin.Animals were observed for tumor growth and mortality rate.Blood was collected to determine hematological and biochemical parameters.Results TAT2 was isolated from an ethanolic extract of 1.tonkinensis leaves.Its structure was determined by MS and NMR spectroscopy,and identified as TAT2.The compound did not show acute toxicity at the highest dose tested(2 OIK) mg/kg body weight).TAT2 exhibited antitumor activity by decreasing tumor growth,increasing the survival rale,and ameliorating some hematological and biochemical parameters at doses of 100 and 200 mg/kg body weight(P<0.05).Conclusions:These results indicate that TAT2 possesses clear antitumor activity.Due to its bioavailability and low toxicity,and the fact that it could be isolated in a large scale from A.tonkinensis leaves,the compound shows promise as a potential anticancer drug.
基金Young Talents Training Project of Jiangxi Academy of Forestry(2015520801).
文摘[Objectives]To explore the response characteristics of chlorophyll and photosynthesis in Styrax tonkinensis under different concentrations of copper and cadmium stress and analyze the internal mechanism of the effects of copper and cadmium pollution stress on photosynthesis.[Methods]Taking seedlings of S.tonkinensis as the research object,the response characteristics of chlorophyll and photosynthesis of S.tonkinensis were compared under different concentrations of copper and cadmium.[Results]With the increase of copper and cadmium treatment concentrations,the chlorophyll content first rose and then fell.With the increase of cadmium concentration,the net photosynthetic rate of S.tonkinensis plants increased from decrease to increase,and the stomatal conductance,transpiration rate and intercellular CO_(2) concentration all increased.[Conclusions]Under the stress of different concentrations of cadmium,the photosynthetic characteristics of S.tonkinensis were not obviously inhibited.Copper stress has inhibitory effect on photosynthesis of S.tonkinensis.
文摘目的基于网络药理学、动物实验及荧光定量PCR技术阐明山豆根改善急性咽炎的分子机制。方法利用中药系统药理学分析平台(Traditional Chinese Medicine systems pharmacology database and analysis platform,TCMSP)数据库收集山豆根的活性成分和作用靶点。通过GeneCards、OMIM、DrugBank和Disgenet数据库获取急性咽炎相关的靶点。筛选获得两者的共同靶标后,利用STRING数据库进行构建蛋白互作网络,采用Metascape平台进行通路分析。同时应用Cytoscape软件构建“中药-疾病-成分-靶点”网络和“中药-疾病-成分-靶点-通路”网络。建立大鼠急性咽炎模型,研究山豆根水提取物对大鼠急性咽炎的影响。利用荧光定量PCR技术研究山豆根对大鼠急性咽炎模型咽部组织关键通路上的关键基因靶点的影响。结果该实验获取山豆根活性成分21个的相关作用靶点509个,获取急性咽炎相关的靶点2167个,山豆根与急性咽炎的共同靶点194个。KEGG通路分析筛选了344条相关信号通路,其中显示IL-17信号通路、NF-kappa B信号通路和白细胞跨内皮迁移通路可能在山豆根改善急性咽炎的过程中起关键作用。动物实验表明,山豆根水提取物低剂量组对急性咽炎治疗效果较好。荧光定量PCR实验结果表明,山豆根低剂量组明显下调了白细胞跨内皮迁移通路ITGB2、PIK3CA、PIK3CD和PTPN11基因的表达水平(P<0.05)。结论山豆根改善急性咽炎具有多成分、多靶点、多通路协同作用的特点。
