Mathematical models have been widely employed for the simulation of growth dynamics of annual crops,thereby performing yield prediction,but not for fruit tree species such as jujube tree(Zizyphus jujuba).The objective...Mathematical models have been widely employed for the simulation of growth dynamics of annual crops,thereby performing yield prediction,but not for fruit tree species such as jujube tree(Zizyphus jujuba).The objectives of this study were to investigate the potential use of a modified WOFOST model for predicting jujube yield by introducing tree age as a key parameter.The model was established using data collected from dedicated field experiments performed in 2016-2018.Simulated growth dynamics of dry weights of leaves,stems,fruits,total biomass and leaf area index(LAI) agreed well with measured values,showing root mean square error(RMSE) values of 0.143,0.333,0.366,0.624 t ha^-1 and 0.19,and R2 values of 0.947,0.976,0.985,0.986 and 0.95,respectively.Simulated phenological development stages for emergence,anthesis and maturity were 2,3 and 3 days earlier than the observed values,respectively.In addition,in order to predict the yields of trees with different ages,the weight of new organs(initial buds and roots) in each growing season was introduced as the initial total dry weight(TDWI),which was calculated as averaged,fitted and optimized values of trees with the same age.The results showed the evolution of the simulated LAI and yields profiled in response to the changes in TDWI.The modelling performance was significantly improved when it considered TDWI integrated with tree age,showing good global(R2≥0.856,RMSE≤0.68 t ha^-1) and local accuracies(mean R2≥0.43,RMSE≤0.70 t ha^-1).Furthermore,the optimized TDWI exhibited the highest precision,with globally validated R2 of 0.891 and RMSE of 0.591 t ha^-1,and local mean R2 of 0.57 and RMSE of 0.66 t ha^-1,respectively.The proposed model was not only verified with the confidence to accurately predict yields of jujube,but it can also provide a fundamental strategy for simulating the growth of other fruit trees.展开更多
Mineral nutrition is the material basis for growth and development,yield and quality of fruit trees. It exerts a great effect on the growth and development,fruit quality,yield,and resistance of fruit trees. On the bas...Mineral nutrition is the material basis for growth and development,yield and quality of fruit trees. It exerts a great effect on the growth and development,fruit quality,yield,and resistance of fruit trees. On the basis of reading a large number of references both at home and abroad,this paper elaborated the relationship between mineral nutrient elements and growth and fruit yield and quality of fruit trees,so as to make proper fertilizer mixing,balance the fertilizer application,promote growth and development of fruit trees,and increase the yield of fruit trees and improve the fruit quality.展开更多
The genus Citrus L. has a long controversial taxonomy history, and a well-resolved molecular phylogeny of the "true citrus fruit trees" group in the future will provide new information for advancing breeding techniq...The genus Citrus L. has a long controversial taxonomy history, and a well-resolved molecular phylogeny of the "true citrus fruit trees" group in the future will provide new information for advancing breeding techniques and developing better conservation strategies. In the present study, three cpDNA fragments (TrnL-TrnF, PsbH-PetB, and TrnS-TrnG) of 30 genotypes chosen from the six genera of the "true citrus fruit trees" group were analyzed. A molecular phylogenetic tree of the "true citrus fruit trees" group "~as reconstructed based on plastid DNA sequences. The results confirmed that the "true citrus fruit trees" group was monophyletic, and thereby the group was divided into genera as previously suggested based on morphological characters. The cpDNA data also suggested that Poncirus might be the first genus separated from the other five genera in the group. The genus Fortunella were of hybrid origin and Citrus might be as its putative paternal parent. The genera Microcitrus, Eremocitrus, and Clymenia were possibly monophyletic and their common ancestor might branch out from Citrus. Furthermore, the phylogenetic relationships within the Citrus genus were discussed.展开更多
Magnesium is an essential nutrient element for the growth and development of fruit trees.It not only participates in photosynthesis of plants,but also promotes physiological and biochemical reactions such as carbohydr...Magnesium is an essential nutrient element for the growth and development of fruit trees.It not only participates in photosynthesis of plants,but also promotes physiological and biochemical reactions such as carbohydrate and protein synthesis,enzyme activation,and reactive oxygen species(ROS)metabolism.Magnesium deficiency in fruit trees will directly influence the growth and development of trees and the improvement of fruit quality.This study mainly reviews the nutritional and physiological mechanism of magnesium in plants and the nutritional diagnosis of magnesium deficiency in fruit trees,in order to provide a theoretical basis for further research on magnesium nutrition in fruit trees,and accordingly provide a certain reference for the application of magnesium fertilizer.展开更多
This research is to control the pests inside fruits and buds by injecting pesticides into the tree with the high-pressure injector. The soluable pesticides injected into the trunk can transported insides to every part...This research is to control the pests inside fruits and buds by injecting pesticides into the tree with the high-pressure injector. The soluable pesticides injected into the trunk can transported insides to every part of the tree to kill pests. The change of pesticides inside the tree was observed. The result showed that injecting pesticides once a year could kill above 80% of the pests which happened once annually. The effect of injection lasts longer than that of applying the pesticide outside. The pesticides injected into the tree are not affected by environment and do not kill natural enemies directly,and the residual amount of pesticides is below the international standard level.展开更多
Second bloom of fruit trees refers to the flowering and fruit-bearing of fruit trees after autumn harvest, which seriously influences the yield and economic benefits of fruit trees. By analyzing the causes of second b...Second bloom of fruit trees refers to the flowering and fruit-bearing of fruit trees after autumn harvest, which seriously influences the yield and economic benefits of fruit trees. By analyzing the causes of second bloom, remedies are given to make scientific preventive measures and realize high-quality and high-yield cultivation of fruit trees.展开更多
Tropical fruit trees constitute important biological resources in the global agrobiodiversity context. Unlike the tropical fruit trees of American and Asian origin, indigenous fruit trees (IFT) of tropical Africa have...Tropical fruit trees constitute important biological resources in the global agrobiodiversity context. Unlike the tropical fruit trees of American and Asian origin, indigenous fruit trees (IFT) of tropical Africa have scarcely achieved the status of international recognition in commodity markets and research arena outside Africa. This paper presented a critical review of the status of IFT in the Tropical African sub-regions (of West Africa, Central Africa, East Africa, Southern Africa and the Indian Ocean Islands) in relation to the introduced naturalised fruit trees from tropical America and Asia, threats to the diversity and sustainable use of IFT, analysis of the opportunities and challenges of developing IFT, as well as targets for crop improvement of the rich IFT of Tropical Africa. Domestication programme via relevant vegetative propagation techniques for priority IFT of the sub-regions was examined and advocated, in addition to the adoption of complementary conservation strategies, including Field GeneBanks in the management of the continent’s IFT diversity.展开更多
Optimum growth and production of fruit crops is strongly linked to managing irrigation water. Various method of estimating tree water requirements have been utilized such as direct and indirect soil, water, and climat...Optimum growth and production of fruit crops is strongly linked to managing irrigation water. Various method of estimating tree water requirements have been utilized such as direct and indirect soil, water, and climatic measurements. Due to differences in fruit tree anatomical and morphological structures and their adaptation to excess and deficit soil water content, such estimates of irrigation water requirements may be more suitable for herbaceous plants but not as accurate for trees. Studies on temperate and tropical fruit trees, using apple (Malus domestica) and star-fruit (Averrhoa carambola), respectively, showed that tree water potential is highly correlated to soil water status. Irrigation based on climate data (ET) and monitoring of soil water resulted in no significant differences in soil or tree water status of orchard-grown fruit trees under temperate and subtropical climatic conditions. The results indicated the need for better understanding and utilization of tree physiological parameters for management of irrigation water of fruit crops. This will ultimately lead to achieving optimum yield and fruit quality while conserving water resources.展开更多
The most recent research findings on the tolerance of fruit trees to cold stress are reviewed from a molecular perspective,including the perception and transduction of low temperature calcium signaling,CBF-dependent m...The most recent research findings on the tolerance of fruit trees to cold stress are reviewed from a molecular perspective,including the perception and transduction of low temperature calcium signaling,CBF-dependent molecular regulatory mechanisms,non-CBF-dependent molecular regulatory mechanisms,and so forth.The objective is to provide a reference basis for further improving the cold resistance of fruit trees and cultivating new varieties of hardy plants.展开更多
A laboratory bioassay was designed to determine the aileiopathic potential of root exudates of three fruit tree species on apple germination. The results showed that root exudates of apple (Malus pumila L.) and pea...A laboratory bioassay was designed to determine the aileiopathic potential of root exudates of three fruit tree species on apple germination. The results showed that root exudates of apple (Malus pumila L.) and peach (Prunus persica L.), each at concentrations of 0.02 and 0.2 mg/L, inhibited germination and radicle growth of apple seeds by 56.7%, 60.7%, 51.5%, and 59.3%, respectively. The corresponding shoot growth inhibition rate was 49.5%, 46.7%, 36. 4%, and 44%, respectively. Root exudates of jujube (Ziziphus jujuba Mill.) had no significant effect on apple seeds. Qualitative determination of root exudates of apple, peach, and jujube tree was developed with gas chromatography-mass spectrometry. The root exudates of apple seedlings mainly contain organic acids, glycol, esters, and benzenphenol derivatives. Peach root exudates contained phenolic acids and benzenphenoi derivatives in addition to two unidentified compounds. The root exudates of jujube did not contain any phenolic acids.展开更多
Tree skeleton could be useful to agronomy researchers because the skeleton describes the shape and topological structure of a tree.The phenomenon of organs’mutual occlusion in fruit tree canopy is usually very seriou...Tree skeleton could be useful to agronomy researchers because the skeleton describes the shape and topological structure of a tree.The phenomenon of organs’mutual occlusion in fruit tree canopy is usually very serious,this should result in a large amount of data missing in directed laser scanning 3D point clouds from a fruit tree.However,traditional approaches can be ineffective and problematic in extracting the tree skeleton correctly when the tree point clouds contain occlusions and missing points.To overcome this limitation,we present a method for accurate and fast extracting the skeleton of fruit tree from laser scanner measured 3D point clouds.The proposed method selects the start point and endpoint of a branch from the point clouds by user’s manual interaction,then a backward searching is used to find a path from the 3D point cloud with a radius parameter as a restriction.The experimental results in several kinds of fruit trees demonstrate that our method can extract the skeleton of a leafy fruit tree with highly accuracy.展开更多
In order to optimize and transform closed mature apple orchards with standard rootstocks and improve the quality of fruit,taking a closed Red Fuji apple orchard as the test object,the effects of different density-redu...In order to optimize and transform closed mature apple orchards with standard rootstocks and improve the quality of fruit,taking a closed Red Fuji apple orchard as the test object,the effects of different density-reducing methods(deinterlacing,removing every other plant in each row,removing every other plant every other row)on the canopy microenvironment,tree structure,leaf photosynthesis and fruit quality were studied.The results showed that different density-reducing methods significantly reduced the orchard coverage and increased the crown transmittance.Among them,the deinterlacing treatment was the best in improving the population structure of the closed orchard,as it reduced the orchard coverage rate by 55.68 percentage points and the canopy transmittance by 82.38 percentage points,compared with the control(CK).Different density-reducing methods all could significantly reduce the branch amount in the closed orchard and optimized the branch composition.The three density-reducing methods decreased the number of branches per plant by 18.96%,12.41%and 19.58%,respectively,compared with the CK.And compared with the CK,the proportion of short branches and leafy branches to the total branches was increased by 17.13%,14.27%and 7.37%,respectively,and the proportion of long branches and developmental branches to the total branches was decreased by 24.47%,18.04%and 10.79%,respectively.The effects of the different density-reducing methods on the temperature,relative light intensity,SPAD and leaf photosynthetic rate in canopies all followed an order of deinterlacing>removing every other plant in each row>removing every other plant every other row>CK,while those on the relative humidity showed an order of deinterlacing>removing every other plant in each row>removing every other plant every other row>CK,while those on the relative humidity showed an order of deinterlacing<removing every other plant in each row<removing every other plant every other row<CK.The average single fruit weight(238.3 g),coloring index(89.2),smoothness index(83.2),soluble solid content(15.1%)and high quality fruit rate(82.4%)of the deinterlacing treatment were higher than those of other treatments,and the values were 18.2%,11.4%,5.85%,26.9%and 25.2%higher than the CK,respectively.The use of dein ̄terlacing to reduce density is the best for improving the microenvironment of closed apple orchards and improving the photosynthetic efficiency and fruit quality.展开更多
Citrus is the typical mycorrhizal fruit tree species establishing symbiosis with arbuscular mycorrhizal (AM) fungi. However, arbuscule development and senescence in colonized citrus roots, especially in response to dr...Citrus is the typical mycorrhizal fruit tree species establishing symbiosis with arbuscular mycorrhizal (AM) fungi. However, arbuscule development and senescence in colonized citrus roots, especially in response to drought stress, remain unclear, which is mainly due to the difficulty in clearing and staining lignified roots with the conventional method. Here, we improved the observation of colonized roots of citrus plants with the sectioning method, which enabled the clear observation of AM fungal structures. Furthermore, we investigated the effects of one week of drought stress on arbuscule development and senescence with the sectioning method. Microscopy observations indicated that drought stress significantly decreased mycorrhizal colonization (F%and M%) although it did not affect plant growth performance. Fluorescence probes (WGA 488 and/or Nile red) revealed that drought stress inhibited arbuscule development by increasing the percentage of arbuscules at the early stage and decreasing the percentages of arbuscules at the midterm and mature stages. Meanwhile, drought stress accelerated arbuscule senescence, which was characterized by the increased accumulation of neutral lipids. Overall, the sectioning method developed in this study enables the in-depth investigation of arbuscule status, and drought stress can inhibit arbuscule development but accelerate arbuscule senescence in the colonized roots of citrus plants. This study paves the way to elaborately dissecting the arbuscule dynamics in the roots of fruit tree species in response to diverse abiotic stresses.展开更多
基金supported by the National Natural Science Foundation of China(41561088 and 61501314)the Science&Technology Nova Program of Xinjiang Production and Construction Corps,China(2018CB020)
文摘Mathematical models have been widely employed for the simulation of growth dynamics of annual crops,thereby performing yield prediction,but not for fruit tree species such as jujube tree(Zizyphus jujuba).The objectives of this study were to investigate the potential use of a modified WOFOST model for predicting jujube yield by introducing tree age as a key parameter.The model was established using data collected from dedicated field experiments performed in 2016-2018.Simulated growth dynamics of dry weights of leaves,stems,fruits,total biomass and leaf area index(LAI) agreed well with measured values,showing root mean square error(RMSE) values of 0.143,0.333,0.366,0.624 t ha^-1 and 0.19,and R2 values of 0.947,0.976,0.985,0.986 and 0.95,respectively.Simulated phenological development stages for emergence,anthesis and maturity were 2,3 and 3 days earlier than the observed values,respectively.In addition,in order to predict the yields of trees with different ages,the weight of new organs(initial buds and roots) in each growing season was introduced as the initial total dry weight(TDWI),which was calculated as averaged,fitted and optimized values of trees with the same age.The results showed the evolution of the simulated LAI and yields profiled in response to the changes in TDWI.The modelling performance was significantly improved when it considered TDWI integrated with tree age,showing good global(R2≥0.856,RMSE≤0.68 t ha^-1) and local accuracies(mean R2≥0.43,RMSE≤0.70 t ha^-1).Furthermore,the optimized TDWI exhibited the highest precision,with globally validated R2 of 0.891 and RMSE of 0.591 t ha^-1,and local mean R2 of 0.57 and RMSE of 0.66 t ha^-1,respectively.The proposed model was not only verified with the confidence to accurately predict yields of jujube,but it can also provide a fundamental strategy for simulating the growth of other fruit trees.
基金Supported by Basic Breeding Project of Shanxi Academy of Agricultural Sciences"Collection,Storage,Evaluation,and Utilization of Crisp Peaches"(YYZJC1420)
文摘Mineral nutrition is the material basis for growth and development,yield and quality of fruit trees. It exerts a great effect on the growth and development,fruit quality,yield,and resistance of fruit trees. On the basis of reading a large number of references both at home and abroad,this paper elaborated the relationship between mineral nutrient elements and growth and fruit yield and quality of fruit trees,so as to make proper fertilizer mixing,balance the fertilizer application,promote growth and development of fruit trees,and increase the yield of fruit trees and improve the fruit quality.
基金supported by the National Natural Science Foundation of China (NSFC, 30671450)
文摘The genus Citrus L. has a long controversial taxonomy history, and a well-resolved molecular phylogeny of the "true citrus fruit trees" group in the future will provide new information for advancing breeding techniques and developing better conservation strategies. In the present study, three cpDNA fragments (TrnL-TrnF, PsbH-PetB, and TrnS-TrnG) of 30 genotypes chosen from the six genera of the "true citrus fruit trees" group were analyzed. A molecular phylogenetic tree of the "true citrus fruit trees" group "~as reconstructed based on plastid DNA sequences. The results confirmed that the "true citrus fruit trees" group was monophyletic, and thereby the group was divided into genera as previously suggested based on morphological characters. The cpDNA data also suggested that Poncirus might be the first genus separated from the other five genera in the group. The genus Fortunella were of hybrid origin and Citrus might be as its putative paternal parent. The genera Microcitrus, Eremocitrus, and Clymenia were possibly monophyletic and their common ancestor might branch out from Citrus. Furthermore, the phylogenetic relationships within the Citrus genus were discussed.
文摘Magnesium is an essential nutrient element for the growth and development of fruit trees.It not only participates in photosynthesis of plants,but also promotes physiological and biochemical reactions such as carbohydrate and protein synthesis,enzyme activation,and reactive oxygen species(ROS)metabolism.Magnesium deficiency in fruit trees will directly influence the growth and development of trees and the improvement of fruit quality.This study mainly reviews the nutritional and physiological mechanism of magnesium in plants and the nutritional diagnosis of magnesium deficiency in fruit trees,in order to provide a theoretical basis for further research on magnesium nutrition in fruit trees,and accordingly provide a certain reference for the application of magnesium fertilizer.
文摘This research is to control the pests inside fruits and buds by injecting pesticides into the tree with the high-pressure injector. The soluable pesticides injected into the trunk can transported insides to every part of the tree to kill pests. The change of pesticides inside the tree was observed. The result showed that injecting pesticides once a year could kill above 80% of the pests which happened once annually. The effect of injection lasts longer than that of applying the pesticide outside. The pesticides injected into the tree are not affected by environment and do not kill natural enemies directly,and the residual amount of pesticides is below the international standard level.
文摘Second bloom of fruit trees refers to the flowering and fruit-bearing of fruit trees after autumn harvest, which seriously influences the yield and economic benefits of fruit trees. By analyzing the causes of second bloom, remedies are given to make scientific preventive measures and realize high-quality and high-yield cultivation of fruit trees.
文摘Tropical fruit trees constitute important biological resources in the global agrobiodiversity context. Unlike the tropical fruit trees of American and Asian origin, indigenous fruit trees (IFT) of tropical Africa have scarcely achieved the status of international recognition in commodity markets and research arena outside Africa. This paper presented a critical review of the status of IFT in the Tropical African sub-regions (of West Africa, Central Africa, East Africa, Southern Africa and the Indian Ocean Islands) in relation to the introduced naturalised fruit trees from tropical America and Asia, threats to the diversity and sustainable use of IFT, analysis of the opportunities and challenges of developing IFT, as well as targets for crop improvement of the rich IFT of Tropical Africa. Domestication programme via relevant vegetative propagation techniques for priority IFT of the sub-regions was examined and advocated, in addition to the adoption of complementary conservation strategies, including Field GeneBanks in the management of the continent’s IFT diversity.
文摘Optimum growth and production of fruit crops is strongly linked to managing irrigation water. Various method of estimating tree water requirements have been utilized such as direct and indirect soil, water, and climatic measurements. Due to differences in fruit tree anatomical and morphological structures and their adaptation to excess and deficit soil water content, such estimates of irrigation water requirements may be more suitable for herbaceous plants but not as accurate for trees. Studies on temperate and tropical fruit trees, using apple (Malus domestica) and star-fruit (Averrhoa carambola), respectively, showed that tree water potential is highly correlated to soil water status. Irrigation based on climate data (ET) and monitoring of soil water resulted in no significant differences in soil or tree water status of orchard-grown fruit trees under temperate and subtropical climatic conditions. The results indicated the need for better understanding and utilization of tree physiological parameters for management of irrigation water of fruit crops. This will ultimately lead to achieving optimum yield and fruit quality while conserving water resources.
基金Supported by Basic Research Fund of Hebei Academy of Agriculture and Forestry Sciences(2024020202)"Three-Three-Three"Talent Project of Hebei Province(C20231157)+2 种基金Science and Technology Innovation Project of Hebei Academy of Agriculture and Forestry Sciences(2022KJCXZX-CGS-7)Hebei Agricultural Industry Research System(HBCT2024170406)Key Research and Development Program of Hebei Province(21326308D-1-2).
文摘The most recent research findings on the tolerance of fruit trees to cold stress are reviewed from a molecular perspective,including the perception and transduction of low temperature calcium signaling,CBF-dependent molecular regulatory mechanisms,non-CBF-dependent molecular regulatory mechanisms,and so forth.The objective is to provide a reference basis for further improving the cold resistance of fruit trees and cultivating new varieties of hardy plants.
文摘A laboratory bioassay was designed to determine the aileiopathic potential of root exudates of three fruit tree species on apple germination. The results showed that root exudates of apple (Malus pumila L.) and peach (Prunus persica L.), each at concentrations of 0.02 and 0.2 mg/L, inhibited germination and radicle growth of apple seeds by 56.7%, 60.7%, 51.5%, and 59.3%, respectively. The corresponding shoot growth inhibition rate was 49.5%, 46.7%, 36. 4%, and 44%, respectively. Root exudates of jujube (Ziziphus jujuba Mill.) had no significant effect on apple seeds. Qualitative determination of root exudates of apple, peach, and jujube tree was developed with gas chromatography-mass spectrometry. The root exudates of apple seedlings mainly contain organic acids, glycol, esters, and benzenphenol derivatives. Peach root exudates contained phenolic acids and benzenphenoi derivatives in addition to two unidentified compounds. The root exudates of jujube did not contain any phenolic acids.
基金This work is supported through grants from the National Natural Science Foundation of China(No.61762013)basic ability improvement project for young and middle-aged teachers in universities of Guangxi province(No.2018KY0078)+1 种基金Science and technology program of Guangxi(No.2018AD19339)Research Fund of Guangxi Key Lab of Multi-Source Information Mining and Security(No.20-A-02-02).
文摘Tree skeleton could be useful to agronomy researchers because the skeleton describes the shape and topological structure of a tree.The phenomenon of organs’mutual occlusion in fruit tree canopy is usually very serious,this should result in a large amount of data missing in directed laser scanning 3D point clouds from a fruit tree.However,traditional approaches can be ineffective and problematic in extracting the tree skeleton correctly when the tree point clouds contain occlusions and missing points.To overcome this limitation,we present a method for accurate and fast extracting the skeleton of fruit tree from laser scanner measured 3D point clouds.The proposed method selects the start point and endpoint of a branch from the point clouds by user’s manual interaction,then a backward searching is used to find a path from the 3D point cloud with a radius parameter as a restriction.The experimental results in several kinds of fruit trees demonstrate that our method can extract the skeleton of a leafy fruit tree with highly accuracy.
基金Supported by Key Research and Development Program of Shandong Province(2017CXGC0210)Agricultural Science and Technology Innovation Project of Shandong Academy of Agricultural Sciences(CXGC2017D01)+3 种基金National Key R&D Program of China(2016YFD0201100)National Natural Science Foundation of China(31600021)Modern Agricultural Industry Technology System of China for Apple(CARS-27)Dongying Science and Technology Program(2015GG0104)
文摘In order to optimize and transform closed mature apple orchards with standard rootstocks and improve the quality of fruit,taking a closed Red Fuji apple orchard as the test object,the effects of different density-reducing methods(deinterlacing,removing every other plant in each row,removing every other plant every other row)on the canopy microenvironment,tree structure,leaf photosynthesis and fruit quality were studied.The results showed that different density-reducing methods significantly reduced the orchard coverage and increased the crown transmittance.Among them,the deinterlacing treatment was the best in improving the population structure of the closed orchard,as it reduced the orchard coverage rate by 55.68 percentage points and the canopy transmittance by 82.38 percentage points,compared with the control(CK).Different density-reducing methods all could significantly reduce the branch amount in the closed orchard and optimized the branch composition.The three density-reducing methods decreased the number of branches per plant by 18.96%,12.41%and 19.58%,respectively,compared with the CK.And compared with the CK,the proportion of short branches and leafy branches to the total branches was increased by 17.13%,14.27%and 7.37%,respectively,and the proportion of long branches and developmental branches to the total branches was decreased by 24.47%,18.04%and 10.79%,respectively.The effects of the different density-reducing methods on the temperature,relative light intensity,SPAD and leaf photosynthetic rate in canopies all followed an order of deinterlacing>removing every other plant in each row>removing every other plant every other row>CK,while those on the relative humidity showed an order of deinterlacing>removing every other plant in each row>removing every other plant every other row>CK,while those on the relative humidity showed an order of deinterlacing<removing every other plant in each row<removing every other plant every other row<CK.The average single fruit weight(238.3 g),coloring index(89.2),smoothness index(83.2),soluble solid content(15.1%)and high quality fruit rate(82.4%)of the deinterlacing treatment were higher than those of other treatments,and the values were 18.2%,11.4%,5.85%,26.9%and 25.2%higher than the CK,respectively.The use of dein ̄terlacing to reduce density is the best for improving the microenvironment of closed apple orchards and improving the photosynthetic efficiency and fruit quality.
基金supported by grants from the Natural Science Foundation of China (Grant No.42077040)the open competition program of top ten critical priorities of Agricultural Science and Technology Innovation for the 14th Five-Year Plan of Guangdong Province (Grant Nos.2022SDZG09,2023SDZG09)+1 种基金the Natural Science Foundation of Guangdong (Grant No.2021B1515010868)the GDAS Project of Science and Technology Development(2021GDASYL-20210103023)。
文摘Citrus is the typical mycorrhizal fruit tree species establishing symbiosis with arbuscular mycorrhizal (AM) fungi. However, arbuscule development and senescence in colonized citrus roots, especially in response to drought stress, remain unclear, which is mainly due to the difficulty in clearing and staining lignified roots with the conventional method. Here, we improved the observation of colonized roots of citrus plants with the sectioning method, which enabled the clear observation of AM fungal structures. Furthermore, we investigated the effects of one week of drought stress on arbuscule development and senescence with the sectioning method. Microscopy observations indicated that drought stress significantly decreased mycorrhizal colonization (F%and M%) although it did not affect plant growth performance. Fluorescence probes (WGA 488 and/or Nile red) revealed that drought stress inhibited arbuscule development by increasing the percentage of arbuscules at the early stage and decreasing the percentages of arbuscules at the midterm and mature stages. Meanwhile, drought stress accelerated arbuscule senescence, which was characterized by the increased accumulation of neutral lipids. Overall, the sectioning method developed in this study enables the in-depth investigation of arbuscule status, and drought stress can inhibit arbuscule development but accelerate arbuscule senescence in the colonized roots of citrus plants. This study paves the way to elaborately dissecting the arbuscule dynamics in the roots of fruit tree species in response to diverse abiotic stresses.