Application of Multi-agent Models to Urban Expansion in Medium and Small Cities: A Case Study in Fuyang City,Zhejiang Province,China

In this study, three-phase satellite images were used to define rules for the allocation of time and space in construction land resources based on a complex adaptive system and game theory. The decision behavior and rules of government agent, enterprise agent and resident agent in construction land growth were explored. A distinctive and dynamic simulation model of construction land growth was built, which integrated multi-agent, GIS technology and RS data and described the interaction among influencing agents, Taking Fuyang City in the Changjiang River Delta as an example, an assessment process for the remote sensing data in construction land and scenario planning was constructed. Repast and ArcGIS were used as simulation platforms. A simulation of the spatial pattern in land-use planning and the setting of scenario planning were conducted by using the incomplete active game, which was based on different natural, social and economic levels. Through this model, a simulation of urban planning space and decision-making for Fuyang City was created. Relevant non-structured problems arising from urban planning management could be identified, and the process and logic of urban planning spatial decision-making could thus be improved. Cell-by-cell comparison showed that the simulation accuracy was over 72%. This model has great potential for use by government and town planners in decision support and technique support in the policy-making process.

The Fuzzy Synthesizing Evaluation of City's Environmental Quality Based on GIOWA Operator:A Case of 13 Cities of Jiangsu Province

In this paper, the generally induced ordered weighted averaging (GIOWA) operator is used to construct the fuzzy evaluating system of the quality of a city’s environment including air, water and noise. Based on the part data of air, water and noise of the 13 cities under the jurisdiction of Jiangsu provincial government in 2002, a case study is given. The evaluating results show that the evaluating system of the quality of a city’s environment based on the GIOWA operator can reflect the condition of pollution controlled and environmental quality of a city in a period of time.

Balance of Datum Land Prices Among Cities Based on the City Gravitation Model and Stochastic Diffusion Equation

A balance of urban datum land prices is achieved to harmonize regional land prices and make the prices truly reflect different economic development levels and land prices among cities. The current piecewise linear interpolation balance method widely used has two drawbacks that always lead to an unsatisfactory balance among some cities. When the excess of land price in the central city to the surrounding zone reaches a certain degree, land price in the circumjacent city is not only consistent with the local land grade and land use level, but also influenced by the diffusion of land price in the central city. Thus, a new balanced scheme of datum land prices based on the city gravitation model and stochastic diffusion equation is brought forward. Finally, the new method is examined in the practice of datum land price balance in Hubei Province, China.

Spatiotemporal Evolution of Urban Heat Islands and Optimization of Spatial Network Construction in the Central Urban Area of Fuzhou City, China

Although accelerated urbanization has led to economic prosperity,it has also resulted in urban heat island effects.Therefore,identifying methods of using limited urban spaces to alleviate heat islands has become an urgent issue.In this study,we assessed the spatiotemporal evolution of urban heat islands within the central urban area of Fuzhou City,China from 2010 to 2019.This assessment was based on a morphological spatial pattern analysis(MSPA)model and an urban thermal environment spatial network constructed us-ing the minimum cumulative resistance(MCR)model.Optimization measures for the spatial network were proposed to provide a theor-etical basis for alleviating urban heat islands.The results show that the heat island area within the study area gradually increased while that of urban cold island area gradually decreased.The core area was the largest of the urban heat island patch landscape elements with a significant impact on other landscape elements,and represented an important factor underlying urban heat island network stability.The thermal environment network revealed a total of 197 thermal environment corridors and 93 heat island sources.These locations were then optimized according to the current land use,which maximized the potential of 1599.83 ha.Optimization based on current land use led to an increase in climate resilience,with effective measures showing reduction in thermal environment spatial network structure and function,contributing to the mitigation of urban heat island.These findings support the use of current land use patterns during urban heat island mitigation measure planning,thus providing an important reference basis for alleviating urban heat island effects.

Function Evolution and Landscape Planning Strategy of Inland Rivers in Beilun Port City of Ningbo

In the history, the main roles of inland rivers in Beilun Port City of Ningbo were desalination,blocking tides, shipping, and flood control. Nowadays, with the continuous spread and deepening ofurbanization, the ecological environment of river courses has been destroyed. In the past, remediationmeasures based on engineering and technology played a certain role, but can not “cure the root cause”. Itshould respect the historical evolution process of river courses, and highlight the ecological service functionand leisure tourism value of river courses from the coordination perspective of urban and rural ecologicalenvironment, economic industries, society and culture in the planning ideas of ecology, production, andlife integration. Four aspects of the measures are as below: protecting and repairing the ecological matrixof river courses;building green space system and maintaining flood control functions through the waternetwork;protecting cultural heritage along the rivers;developing waterfront leisure tourism scenic area.

Impact of coupling coordination development of port and city environments on urban economic competitiveness:Evidence from the coastal port cities in eastern China

Promoting the coupling coordination development of port and its hinterland city environments is an important way to improve urban economic competitiveness.Based on relevant data of 13 coastal port cities in eastern China from 2000 to 2018,this study explores the coupling coordination development of port and city environments and its impact on urban economic competitiveness by constructing the coupling coordination degree model and the panel threshold model.The research results show that:(1)In terms of the coupling coordination development of port and city environments,most coastal ports and their hinterland cities are in a state of moderate or serious disorder.Overall,the degree of coupling coordination of port and city environments needs to be further improved;(2)The coupling coordination degree of port and city environments has a significant impact on urban economic competitiveness,and this effect gradually increases with the development of the ports and the urban economy.Among the variables that impact the urban economic competitiveness,fixed assets investment and foreign trade are significant factors that can enhance urban economic competitiveness.(3)At present,there is a“U-shaped”relationship between the coupling coordination degree of port-city environments and the urban economic competitiveness.This relationship lies on the right side of the inflection point of the“U-shaped”curve.Therefore,following the concept of assigning priority to ecological development,expanding fixed assets investment and actively developing foreign trade can further enhance the urban economic competitiveness.

Role of glutathione transferase in phase separation of FUS and TAF15 in neurons

Amyotrophic lateral sclerosis(ALS)is a progressive neurodegenerative disorder characterized by the degeneration of motor neurons in the brain and spinal cord,leading to muscle weakness,paralysis,and ultimately death(Cleveland and Rothstein,2001).Frontotemporal lobar degeneration(FTLD)is a neurodegenerative disease affecting the frontal and temporal lobes of the brain,leading to changes in behavior,personality,and language(Van Langenhove et al.,2012).Both ALS and FTLD are classified as proteinopathies in which abnormal protein aggregation and accumulation in neurons contribute to the disease pathogenesis.Fused in sarcoma(FUS)is a DNA/RNA-binding protein involved in various cellular processes,including transcriptional regulation,RNA splicing,and DNA repair.Mutations in the FUS gene have been linked to familial ALS,highlighting the importance of FUS in the disease pathogenesis(Vance et al.,2009).In ALS and FTLD,aberrant post-translational modifications(PTMs)of FUS,such as phosphorylation,acetylation,and methylation,have been implicated in the promotion of FUS aggregation and neurotoxicity(Choi et al.,2023).Therefore,understanding the regulatory mechanisms of FUS PTMs is crucial for developing targeted therapies against these diseases.

Research of Future Directions and Considerations for Implementation of the Forest City Strategy

A “Forest City” (FC) is an urban area that has a significant amount of forest cover. It is now a green urban development strategy that is supported by numerous nations. This essay compares the many FC implementation strategies used in developed and developing countries and explores potential future paths for this tactic. The variations between FC in terms of measurement targets, air purification, street trees, and forestry development are thoroughly compared in this research. This essay goes on to explore FC’s potential in the future regarding policy changes and the environment based on this comparison. Therefore, this essay focuses on the necessity of considering industrial innovation, encouraging biodiversity, lowering greenhouse gas emissions, paying attention to forest restructuring, and being more responsive to the issues provided by urbanization in the future global implementation of FC.

Application Strategies of Spatial Refinement Design in Ancient Cities Supported by Digital Te-Zhaoyu Ancient City in Qixian County as an Example

Digital technology provides a method of quantitative investigation and data analysis for contemporary landscape spatial analysis,and related research is moving from image recognition to digital algorithmic analysis,providing a more scientific and macroscopic way of research.The key to refinement design is to refine the spatial design process and the spatial improvement strategy system.Taking the ancient city of Zhaoyu in Qixian County,Shanxi Province as an example,(1)based on obtaining the integrated data of the ancient city through the drone tilt photography,the style and landscape of the ancient city are modeled;(2)the point cloud data with spatial information is imported into the point cloud analysis platform and the data analysis is carried out from the overall macroscopic style of the ancient city to the refinement level,which results in the formation of a more intuitive landscape design scheme,thus improving the precision and practicability of the landscape design;(3)Based on spatial big data,it starts from the spatial aggregation level,spatial distribution characteristics and other evaluation index system to achieve the refinement analysis of the site.Digital technology and methods are used throughout the process to explore the refined design path.

Spatio-temporal Evolution Characteristics and Driving Forces of Winter Urban Heat Island:A Case Study of Rapid Urbanization Area of Fuzhou City,China

Under the influence of anthropogenic and climate change,the problems caused by urban heat island(UHI)has become increasingly prominent.In order to promote urban sustainable development and improve the quality of human settlements,it is significant for exploring the evolution characteristics of urban thermal environment and analyzing its driving forces.Taking the Landsat series images as the basic data sources,the winter land surface temperature(LST)of the rapid urbanization area of Fuzhou City in China was quantitatively retrieved from 2001 to 2021.Combing comprehensively the standard deviation ellipse model,profile analysis and GeoDetector model,the spatio-temporal evolution characteristics and influencing factors of the winter urban thermal environment were systematically analyzed.The results showed that the winter LST presented an increasing trend in the study area during 2001–2021,and the winter LST of the central urban regions was significantly higher than the suburbs.There was a strong UHI effect from 2001 to 2021with an expansion trend from the central urban regions to the suburbs and coastal areas in space scale.The LST of green lands and wetlands are significantly lower than croplands,artificial surface and unvegetated lands.Vegetation and water bodies had a significant mitigation effect on UHI,especially in the micro-scale.The winter UHI had been jointly driven by the underlying surface and socio-economic factors in a nonlinear or two-factor interactive enhancement mode,and socio-economic factors had played a leading role.This research could provide data support and decision-making references for rationally planning urban layout and promoting sustainable urban development.

Reflection on the Integrated Development of Marine Fisheries and Tourism Based on the Transformation of Fishery Facilities: A Case Study of Ningde City of Fujian Province, China

Based on the analysis on the current situation of marine fishery resources and development in Ningde City,this paper plans a spatial pattern for the integration of marine fishery and tourism of the“one belt,three zones,and multiple points”,designs key projects,analyzes the existing problems in the integrated development of fishery and tourism,and proposes relevant countermeasures and suggestions.

Transitional Road of Resources-exhausted Cities to Low-carbon Economy:Tourism Resources Development——A Case Study of Gejiu City

Tourism is of vital practical significance to low-carbon economic transition of resources-exhausted cities.By taking Gejiu City of Yunnan Province for example,the paper discussed that Gejiu City as the resources-exhausted city should positively develop tourism,particularly industrial heritage tourism,treating tourism development as the breakthrough and motive power of low-carbon economic transition of resources-exhausted cities,so as to walk out a unique road of tourism promoting low-carbon economic development.

Research on Budding "Villages in Cities" during Urbanization Process in Lanzhou City of China——A Case Study of Chaijiahe Village,China

By introducing the theory of suburb urbanization,it was considered that budding villages in cities emerged with the close relation between city and suburb during rapid urbanization process.The paper had briefly analyzed development of villages in cities in China.By taking villages in Lanzhou City for example,it had studied geological location,appearance,spatial structure,economy and demographic feature of budding villages in cities.Furthermore,with Chaijiahe Village of Xiguoyuan Town as case study,empirical study had been conducted and development thought of Chaijiahe Village as budding village in city had been discussed.It emphasized that it should control the development scale of villages,economically and intensively utilize lands;develop industrialized high-efficiency agriculture and leisure agriculture,develop lily economy and explore the potential of characteristic industries;realize integration of urban and rural development;strive to develop education,improve villages' quality and cultivate villagers of new generation who possessed pioneering spirit and modern agriculture technology.On this basis,it summarized approaches to realize sustainable development of budding villages in cities.

Research on Landscape Ecology Mode of New Resources-based Cities—A Case Study of Yulin City of Shaanxi Province in China

Based on the overall understanding of new cities developing based on resources,by taking Yulin City of Shaanxi Province for example and combining with relevant statistical data,main problems existing in resources development have been pointed out,covering simple resources industrial structure and serious wastes in resources development;insufficient intensive processing of products and low level of resources integrated utilization;and deteriorative regional eco-environment.On this basis,a new mode of landscape ecology of resource-based cities have been proposed,emphasizing constructing new green energy industrial development mode from the perspective of microscopic view;ecological industrial park of circular economy from the perspective of mesoscopic view;and the overall ecological recovery mode of the mining area from the perspective of macroscopic view.It hopes to give a vital inspiration to the sustainable development of new resources-based cities.

Research on Ecological Livable Urban Construction in Small and Medium Cities of China——A Case Study of Huaihua City

The paper has analyzed the concept of ecological livable city,considered that small and medium cities as the major pattern of China's urbanization are more likely to become ecological livable cities compared with large cities.Based on the actual situation of Huaihua City,it has pointed out its advantages in transport,ecology,foundation and national characteristic,and analyzed existing problems in the construction of ecological livable city from the perspective of urban eco-environment.It considers that its urban ecological planning is to be perfect,urban environment pollution is still serious and people's consciousness for environmental protection is to be improved.On this basis,relevant suggestions have been proposed for ecological livable urban construction in Huaihua City,emphasizing it should perfect urban ecological planning,enhance environmental pollution treatment,intensify environmental protection education,and improve people's consciousness for environmental protection.

Investigation on the Characteristics of the Senior Citizens' Activities in the Parks and Greenbelts——A Case Study of Nanjing City

From the perspective of senior citizens,questionnaire survey and field investigation were carried on and analyzed in three parks in Nanjing City.The results showed that outdoor activities are the principal ways for the senior citizens to spend their time,the parks near home is their prior choice,and the radiation range of the park and greenbelt mostly is no more than 2 km;most of the senior citizens come to parks on foot and the parks are always within 30 minutes walking distance;the senior citizens always have fixed partners and groups for their exercise-dominant activities.

Application of Fussy Decision-making in Selecting the Optimal Variety from City Flower Candidates

Fuzzy similar priority ratio is adopted to select the optimal variety from 6 city flower candidates in a certain city,i.e.Nelumbo nucifera x1,Prunus persica Batsch.var.duplex Rehd.x2,Rosa chinensis Jacq.x3,Dendranthema morifolium x4,Jassminum nudiflorum Lindl.x5 and Prunus mume x6.The results show that the priority sequence of 6 candidates was x1,x6,x5,x3,x4 and x2.

Study on the Functional Division of Agricultural Sightseeing in Handan City

Agricultural sightseeing in a new tourism pattern integrates ecological tourism functions into ecological agriculture and rural cultures. Handan City is located in southern Henan Province with excellent geographical conditions. In 2006-2008, agricultural sightseeing has achieved primary development in Handan City, and gradually become an indispensable component of the tourism market in this city. Promoting agricultural sightseeing in Handan City as well as the upgrading of its agriculture will be enhanced, which can be transformed from traditional and unitary function into the comprehensive functions of production, ecology, tourism, culture and education, thus is favorable for promoting the further development of tourism industry, enriching tourism products, realizing the sustainable development of agricultural tourism. Besides, surplus rural labors will be offered more opportunities, and industrial structure in rural areas will also be adjusted properly. Through dividing agricultural sightseeing in Handan City into the eco-agricultural sightseeing zone in the west, metropolis agricultural sightseeing zone in the middle, and economic agricultural sightseeing in the east, the authors clearly defined their ranges, orientation of typical functions, dominant development modes as well as projects, so as to figure out the reasonable development pattern for agricultural sightseeing in Handan City, specify its development context, and provide theoretical basis for the future development of agricultural sightseeing in this city.

薏苡仁油联合5-FU对结肠癌HCT-116细胞株增殖、凋亡的影响

目的:分析薏苡仁油联合5-氟尿嘧啶(5-fluorouracil,5-FU)对结肠癌HCT-116细胞株增殖、凋亡的影响及其作用机制。方法:采用四甲基偶氮唑盐(methyl thiazolyl tetrazdium,MTT)法测定不同浓度薏苡仁油、5-FU以及薏苡仁油+5-FU对人结肠癌HCT-116细胞株的生长抑制效应;采用流式细胞术检测细胞凋亡情况;采用逆转录聚合酶链反应(RT-PCR)检测生存蛋白(Survivin)mRNA表达水平。结果:薏苡仁油、5-FU、5-FU+薏苡仁油均对人结肠癌HCT-116细胞增殖产生一定抑制作用,且薏苡仁油浓度越高,作用时间越长,细胞增殖抑制率越高,3组比较差异有统计学意义(P<0.05);与薏苡仁油组及5-FU组相比,5-FU+薏苡仁油组细胞增殖抑制率更高,差异有统计学意义(P<0.05)。薏苡仁油组、5-FU组、5-FU+薏苡仁油组的细胞凋亡率均高于空白对照组,差异有统计学意义(P<0.05);薏苡仁油浓度越高,细胞凋亡率也越高,差异有统计学意义(P<0.05);5-FU+薏苡仁油组细胞凋亡率高于5-FU组及薏苡仁油组,差异有统计学意义(P<0.05)。与空白对照组相比,不同浓度薏苡仁油组Survivin mRNA表达降低,差异有统计学意义(P<0.05);5-FU组Survivin mRNA表达升高,差异有统计学意义(P<0.05);5-FU+薏苡仁油组Survivin mRNA表达较5-FU组降低,差异有统计学意义(P<0.05)。结论:薏苡仁油能抑制结肠癌HCT-116细胞增殖,诱导细胞凋亡,与5-FU联合应用能发挥药物协同作用,其作用机制可能与调控Survivin mRNA表达有关。

SLC6A9对结直肠癌细胞生长和对5-FU药物敏感性的影响

目的:研究溶质载体家族6成员9(solute carrier family 6 member 9,SLC6A9)表达对结直肠癌细胞增殖、迁移和5-氟尿嘧啶(5-fluorouracil,5-FU)药物敏感性的影响。方法:TCGA数据库分析、实时荧光定量PCR和Western blot分析检测SLC6A9在结肠癌组织、正常结肠细胞系(NCM460)和结直肠癌细胞系(SW620、HCT116、HT29、Lovo和SW480)中的表达。将SCL6A9过表达质粒及阴性对照(SLC6A9 OE、Vector)转染HT29细胞,将SCL6A9小干扰RNA及阴性对照(SLC6A9 siRNA1#、siRNA2#和Scramble)转染SW620细胞。划痕愈合实验和Transwell实验检测各组细胞的迁移、侵袭能力。Western blot和细胞免疫荧光检测EMT相关蛋白E-cadherin、Vimentin的表达水平。利用CCK-8法和构建裸鼠移植瘤模型检测SLC6A9过表达对结直肠癌细胞5-FU药物敏感性的影响。结果:与正常结肠组织和NCM460细胞相比,SLC6A9在结肠癌组织和结直肠癌细胞系中低表达(均P<0.05)。SLC6A9过表达引起E-cadherin蛋白表达增加,Vimentin蛋白水平降低,抑制结直肠癌细胞的迁移、侵袭(P<0.05)。SLC6A9低表达引起E-cadherin蛋白表达降低,Vimentin蛋白水平增加,促进结直肠癌细胞的迁移、侵袭能力(P<0.05)。SLC6A9过表达提高了5-FU的药物敏感性,并使肿瘤生长缓慢,质量减轻(P<0.05)。而SLC6A9低表达降低了5-FU的药物敏感性(P<0.05)。结论:SLC6A9过表达能够抑制结直肠癌细胞的迁移、侵袭和EMT进程,并增强5-FU对结直肠癌细胞的药物敏感性。