Objective:To investigate the effect of Wei’s triple nine needling therapy on the N2-P2 wave of the flash visual evoked potential(FVEP)in rats of the of the transverse directional pulling model.Methods:Thirty-six Wist...Objective:To investigate the effect of Wei’s triple nine needling therapy on the N2-P2 wave of the flash visual evoked potential(FVEP)in rats of the of the transverse directional pulling model.Methods:Thirty-six Wistar rats were randomly grouped,nine were in normal control group without any treatment,and the remaining 27 were surgically modelled in the right eyes.Eighteen of these rats were randomly divided into a Wei’s triple nine needling therapy group and a model group of nine rats each after the TON model was made using the transverse quantitative retraction method.The other 9 rats were sham-operated,and only the optic nerve was exposed without retraction.On the next day of modelling,the Wei’s triple nine needling therapy group was treated with Wei’s triple nine needling therapy for 20 minutes/1 time/1 day for 14 days.The model group,sham-operated group and normal control group were not intervened.Wei's triple nine acupoints were represented as follows:1st link:"Jingming"(BL1)and"Chengqi"(ST1);2nd link:"Sizhukong"(SJ23)penetrating"Taiyang"(EX-HN5);and the third:"Fengchi"(GB 20)and"Taichong"(LV3).The FVEP of each group was observed on 1d,7d and 14d.The FVEP of each group was observed on 1d,7d and 14d.Results:Compared with the model group,the N2 wave latency and P2 wave latency were shortened in the Wei’s triple nine needling therapy on 1d(P<0.05,P<0.01);on 7d,their N2 wave latency was shortened(P<0.01)and the N2-P2 wave amplitude was increased compared with the model group(P<0.05);on 14d,their P2 wave latency was shortened(P<0.05)and the N2-P2 wave amplitude was increased.There was no statistical difference in N2 wave latency,P2 wave latency and N2-P2 wave amplitude in the sham-operated group on 1d,7d and 14d(P>0.05).The delayed N2 and P2 wave latencies in the model group did not improve from 1d to 14d(P>0.05)and the amplitude decreased throughout,showing a significant difference on 14d compared to 1d(P<0.05).In contrast,the N2 wave latency in Wei’s triple nine needling therapy group was not significantly different until 7d to 14d(P<0.05);its P2 wave latency,although significantly delayed from 1d to 7d(P<0.05),recovered on 14d and reached a level that was not statistically different from 1d and 7d(P>0.05).There was also no significant decrease in N2-P2 wave amplitude between 1d and 14d in Wei’s triple nine needling therapy group(P>0.05).Conclusion:In this experiment,the TON rat model was successfully established by the transverse quantitative retraction method,and the treatment of TON rats with Wei’s triple nine needling therapy reduced the P2 wave delay of the FVEP electrophysiological signal and increased the N2-P2 amplitude,which had a certain positive effect on the repair of optic nerve injury,probably related to its effect of improving the conduction function of the optic nerve and protecting the retinal ganglion cells that had not been degenerated and necrosed.展开更多
Buffon’s needle experiment was originally devised to get the value of π. With the advent of computers, Buffon’s needle algorithm has been used pedagogically as an example of Monte Carlo methods in introduction clas...Buffon’s needle experiment was originally devised to get the value of π. With the advent of computers, Buffon’s needle algorithm has been used pedagogically as an example of Monte Carlo methods in introduction classes, and there are many Buffon’s needle algorithm implementations available on the internet. However, for the calculation of π, the exact value of π is used in the programs for Buffon’s needle angle sampling, and hence the example is not demonstrated correctly. This brief note presents a random angle sampling algorithm for the Buffon’s needle. We then compare the Buffon’s needle and Hit-and-Miss integration algorithms using Monte Carlo laboriousness comparison, and find that the Hit-and-Miss algorithm is superior.展开更多
Objective This study examined the research status and development process of FU Qingzhu’s Obstetrics and Gynecology(Fu Qing Zhu Nv Ke,《傅青主女科》,FQZNK)in the past 40 years with bibliometrics and visual analysis.M...Objective This study examined the research status and development process of FU Qingzhu’s Obstetrics and Gynecology(Fu Qing Zhu Nv Ke,《傅青主女科》,FQZNK)in the past 40 years with bibliometrics and visual analysis.Methods Retrieved all related literature in the research field of FQZNK from the domestic and foreign databases:China National Knowledge Infrastructure(CNKI),China Science and Technology Journal Database(VIP),Wanfang Database,and Web of Science(WOS)core database,including Science Citation Index Expanded(SCIE),Social Sciences Citation Index(SSCI),and Arts&Humanities Citation Index(A&HCI).The search range was from January 1,1980 to March 10,2021.In addition,bibliometrics and CiteSpace 5.7.R2 software were used to analyze literature types,published journals,cited literature,the number of author publica-tions,co-author networks,co-institution networks,keyword co-occurrence networks,keyword clusters,and keyword bursts.Results A total of 678 valid records were included in the final dataset.Literature types,high publication journals,highly cited literature,high-yield institutions,high-yield research teams,and high-productivity scholars in this research field were found through bibliometrics.Liter-ature types can be divided into four categories,among which 451 are theoretical studies on academic thoughts of FQZNK,accounting for 66.5%of the included journals.The Journal of Shanxi Traditional Chinese Medicine had the largest volume of published articles(61),ac-counting for 9.0%of the total number of the included journals.The most cited literature was ZHOU Mingxin’s article“Using the quantitative method to discuss author’s authenticity and formula characteristics of FU Qingzhu’s Obstetrics and Gynecology”,which was cited 94 times.Hunan University of Chinese Medicine,the institution with the most publications,published 45 articles,and YOU Zhaoling,the most published author,published 33 articles.Moreover,it was found that most high-yield researchers came from high-yield institutions and that Hun-an University of Chinese Medicine had the most research on FQZNK.Keyword co-occur-rence analysis revealed that the keyword“FQZNK”had the highest frequency(597 times)and the highest centrality(1.00).Keyword cluster analysis used the Log-Likelihood Ratio(LLR)al-gorithm to form eleven important clusters:#0 treatment aiming at its root causes,#1 gynecopathy,#2 Siwu Decoction(四物汤),#3 FU Qingzhu,#4 post-partum,#5 infertility,#6 dysmenorrhea,#7 sterility,#8 coordinate the heart and kidney,#9 Danggui Buxue Decoction(当归补血汤),and#10 treatment.It was found that the prescriptions of FQZNK were studied mainly before 2000,the theoretical studies were mainly conducted before 2010,and its clinic-al application was mainly explored from 2010 until now.Diseases such as dysmenorrhea,morbid vaginal discharge,infertility,metrorrhagia,and polycystic ovarian syndrome(PCOS)have recently become popular topics in this field.Conclusion The current study provides more scientific,accurate,and comprehensive sci-entific support for further research and development of traditional Chinese medicine(TCM)in FQZNK.With this foundation,people can use burst detection to ascertain the current hot-spots in research,get their development trends,and forecast future research directions.In ad-dition,infertility,morbid vaginal discharge,flooding,and PCOS treatments based on TCM syndrome differentiation are currently popular research topics for FQZNK.展开更多
In this study,six kinetics models of indomethacin hydrophilic gel patch transdermal in vitro release was established,including zero-level,first-order,Higuchi-level,Ritger-Peppas,Weibull and Hixcon-Crowell dynamic equa...In this study,six kinetics models of indomethacin hydrophilic gel patch transdermal in vitro release was established,including zero-level,first-order,Higuchi-level,Ritger-Peppas,Weibull and Hixcon-Crowell dynamic equations.The chemical permeation enhancers,including 3%and 5%Azone,and iontophoresis were used as the control.Transdermal diffusion tests were performed in vitro and indomethacin was quantified by high performance liquid chromatography system.The transdermal parameter of the Higuchi and Weibull dynamic equations,indicated that Fu’s cupping therapy(FCT)could significantly improve Higuchi and Weibull kinetic parameters in vitro transdermal,increased transdermal rate and permeability coefficient,reduced lagging time.Additionally,statistical analysis speculated the skin barrier function could be restored after 46 h treatment.Hence,as a new physical transdermal drug delivery technology,transdermal permeation effects produced by FCT are obvious,which has the characteristics of traditional Chinese medicine and has important clinical application value.展开更多
The great Chinese poet Du Fu＇s poetry in the Dang Dynasty has important significance in the development of the world culture. Confucianism is the main theme of his poetry, which penetrates connotation of benevolence,...The great Chinese poet Du Fu＇s poetry in the Dang Dynasty has important significance in the development of the world culture. Confucianism is the main theme of his poetry, which penetrates connotation of benevolence, love of all life and self-cultivation. The famous poet Sun Dayu strived to highlight ideological content and the essence of Confucianism in Du Fu＇s poetry through translation. On the basis of deep understanding of the source text and careful choice of words, Sun Dayu mainly used literal translation, free translation and explanation in order to make the target readers know the spirit of Confucianism. His translation activities showed solicitude for national destiny and historical responsibility.展开更多
In this paper, the background, evolution, basic meaning, clinical application and the detail operating procedures of the differential insertion depth in filiform needle acupuncture were discussed based on the classica...In this paper, the background, evolution, basic meaning, clinical application and the detail operating procedures of the differential insertion depth in filiform needle acupuncture were discussed based on the classical expositions of the Yellow Emperor’s Canon of Medicine. It is believed that the differential insertion depth reflects the basic idea of expelling the evil Qi from the body in the application of traditional acupuncture. Since the site of evil invasion has different shades, the position of evil Qi and correct differentiation has become the operation key points of needle insertion. Apart from this, the Yellow Emperor’s Canon of Medicine has further associated the clinical application of filiform needle insertion depth with the seasonal change of Yin and Yang, the body built of the patients, the nature of the diseases, the heat or cold pathogenic factors of the illness, the excess and deficiency of the patient, and the reinforcing and reducing function of acupuncture. These elaborations have greatly enriched the basic content of acupuncture and laid a systematic theoretical foundation of filiform needle operation. The differential insertion depth in acupuncture has its specific meaning, the emphasis of insertion depth of filiform needle with its differentiated clinical implication exemplifies the perceptual thinking features of traditional acupuncture and typical reveals the uniqueness of Chinese civilization.展开更多
Introduction: Triple immunohistochemical (IHC) stains including antibodies specific for alpha-methylacyl-CoA-racemase and basal cell markers have been a valuable aid in accurate identification of prostate carcinoma. H...Introduction: Triple immunohistochemical (IHC) stains including antibodies specific for alpha-methylacyl-CoA-racemase and basal cell markers have been a valuable aid in accurate identification of prostate carcinoma. However, accurate quantification of minuscule areas of prostate carcinoma in biopsy specimens can often be a challenge. Here we assessed the diagnostic value and quantitative use of automated digital image analysis on triple IHC stained prostate needle biopsies. Methods: Twelve cases of prostate needle biopsy material including 75 needle cores were stained with triple-antibody cocktail (P504S + 34βE12 + p63). Slides were digitally scanned with the APERIO digital image analyzer and evaluated with the GENIE pattern and color recognition digital image analysis that we developed. A slide with known areas of adenocarcinoma, high grade prostatic intraepithelial neoplasia (PIN), benign glands and stroma was used as a training set for the automated digital image analysis platform. Results: Among 75 needle biopsy cores, 19 (25.33%) contained adenocarcinoma by histology. Digital image analysis recognized adenocarcinoma in 95% of these needle biopsies. The average area of the needle biopsy was 7.63 mm2 and overall the average area of tumor was 0.196 mm2. The smallest area of tumor recognized by the program was 0.0022 mm2 (0.0363% of the core) and the largest was 0.62 mm2 (8.17% of the core) among needle core biopsies. False positives resulted from areas of high grade PIN with patchy basal cells. The false negative was caused by uneven AMACR staining in one area of adenocarcinoma. Digital recognition of areas of interest was improved by three successive image analysis training which increased the sensitivity and specificity from 83% and 89% to 90% and 93%, respectively. Conclusions: Digital image analysis in concert with IHC triple staining may be useful for accurate detection and quantitative analysis of small foci of prostatic adenocarcinoma. Defining methods to increase the sensitivity and specificity of quantitative automated digital image analysis will likely evolve as an area of investigation. Future automated digital scanning and innovative pattern and color recognition technologies may open avenues for classifying a variety of prostate lesions.展开更多
Objective: To study the correlation of S100A13 and FOXA1 expression with cell cycle and cell invasion in fine needle aspiration thyroid carcinoma tissue. Methods: Patients who received ultrasound-guided thyroid nodule...Objective: To study the correlation of S100A13 and FOXA1 expression with cell cycle and cell invasion in fine needle aspiration thyroid carcinoma tissue. Methods: Patients who received ultrasound-guided thyroid nodule fine needle aspiration in Haiyang People's Hospital between April 2015 and February 2017 were selected, and the tissues were divided into malignant thyroid tissue and benign thyroid nodules according to the pathological results after biopsy. The expression of S100A13, FOXA1, cell cycle molecules and cell invasion molecules were measured. Results: S100A13, FOXA1, CDK2, CyclinD1, MCM2, MCM7, SKP2, CLOCK, STAT3, STAT5, N-cadherin, MT1-MMP and ADAM17 mRNA expression in thyroid carcinoma tissue were significantly higher than those in benign thyroid nodule;CDK2, CyclinD1, MCM2, MCM7, SKP2 and CLOCK mRNA expression in thyroid carcinoma tissue with high FOXA1 expression were significantly higher than those in thyroid carcinoma tissue with low FOXA1 expression;STAT3, STAT5, N-cadherin, MT1-MMP and ADAM17 mRNA expression in thyroid carcinoma tissue with high S100A13 expression were significantly higher than those in thyroid cancer tissue with low S100A13 expression. Conclusions: High expression of S100A13 and FOXA1 in thyroid carcinoma can promote cell invasion and cell cycle progression.展开更多
目的观察浮针疗法治疗早中期膝骨关节炎对关节软骨厚度的影响。方法将60例早中期膝骨关节炎患者随机分为治疗组和对照组,每组30例。治疗组采用浮针治疗,对照组采用电针治疗。观察两组治疗前后疼痛视觉模拟量表(visual analog scale,VAS...目的观察浮针疗法治疗早中期膝骨关节炎对关节软骨厚度的影响。方法将60例早中期膝骨关节炎患者随机分为治疗组和对照组,每组30例。治疗组采用浮针治疗,对照组采用电针治疗。观察两组治疗前后疼痛视觉模拟量表(visual analog scale,VAS)评分、疼痛开始缓解时间、Lysholm膝关节功能评分、平衡功能及股骨内侧和外侧髁软骨厚度的变化。结果治疗组疼痛开始缓解时间短于对照组(P<0.05)。治疗后,两组VAS评分以及平衡功能的横向标准差和前后标准差均较治疗前降低(P<0.05),Lysholm膝关节功能评分、平衡功能的稳定极限均较治疗前升高(P<0.05)。治疗后3个月,两组VAS评分较治疗前降低(P<0.05),Lysholm膝关节功能评分较治疗前升高(P<0.05),膝关节内外侧髁软骨厚度均较治疗前增加(P<0.05)。治疗后和治疗后3个月,治疗组VAS评分均低于对照组(P<0.05),Lysholm膝关节功能评分均高于对照组(P<0.05)。治疗后,治疗组横向标准差低于对照组(P<0.05),稳定极限高于对照组(P<0.05)。结论浮针治疗早中期膝骨关节炎起效速度优于电针治疗,能有效缓解疼痛症状及提高膝关节功能,可改善软骨损伤。展开更多
基金National Natural Science Foundation of China(No.81904258)。
文摘Objective:To investigate the effect of Wei’s triple nine needling therapy on the N2-P2 wave of the flash visual evoked potential(FVEP)in rats of the of the transverse directional pulling model.Methods:Thirty-six Wistar rats were randomly grouped,nine were in normal control group without any treatment,and the remaining 27 were surgically modelled in the right eyes.Eighteen of these rats were randomly divided into a Wei’s triple nine needling therapy group and a model group of nine rats each after the TON model was made using the transverse quantitative retraction method.The other 9 rats were sham-operated,and only the optic nerve was exposed without retraction.On the next day of modelling,the Wei’s triple nine needling therapy group was treated with Wei’s triple nine needling therapy for 20 minutes/1 time/1 day for 14 days.The model group,sham-operated group and normal control group were not intervened.Wei's triple nine acupoints were represented as follows:1st link:"Jingming"(BL1)and"Chengqi"(ST1);2nd link:"Sizhukong"(SJ23)penetrating"Taiyang"(EX-HN5);and the third:"Fengchi"(GB 20)and"Taichong"(LV3).The FVEP of each group was observed on 1d,7d and 14d.The FVEP of each group was observed on 1d,7d and 14d.Results:Compared with the model group,the N2 wave latency and P2 wave latency were shortened in the Wei’s triple nine needling therapy on 1d(P<0.05,P<0.01);on 7d,their N2 wave latency was shortened(P<0.01)and the N2-P2 wave amplitude was increased compared with the model group(P<0.05);on 14d,their P2 wave latency was shortened(P<0.05)and the N2-P2 wave amplitude was increased.There was no statistical difference in N2 wave latency,P2 wave latency and N2-P2 wave amplitude in the sham-operated group on 1d,7d and 14d(P>0.05).The delayed N2 and P2 wave latencies in the model group did not improve from 1d to 14d(P>0.05)and the amplitude decreased throughout,showing a significant difference on 14d compared to 1d(P<0.05).In contrast,the N2 wave latency in Wei’s triple nine needling therapy group was not significantly different until 7d to 14d(P<0.05);its P2 wave latency,although significantly delayed from 1d to 7d(P<0.05),recovered on 14d and reached a level that was not statistically different from 1d and 7d(P>0.05).There was also no significant decrease in N2-P2 wave amplitude between 1d and 14d in Wei’s triple nine needling therapy group(P>0.05).Conclusion:In this experiment,the TON rat model was successfully established by the transverse quantitative retraction method,and the treatment of TON rats with Wei’s triple nine needling therapy reduced the P2 wave delay of the FVEP electrophysiological signal and increased the N2-P2 amplitude,which had a certain positive effect on the repair of optic nerve injury,probably related to its effect of improving the conduction function of the optic nerve and protecting the retinal ganglion cells that had not been degenerated and necrosed.
文摘Buffon’s needle experiment was originally devised to get the value of π. With the advent of computers, Buffon’s needle algorithm has been used pedagogically as an example of Monte Carlo methods in introduction classes, and there are many Buffon’s needle algorithm implementations available on the internet. However, for the calculation of π, the exact value of π is used in the programs for Buffon’s needle angle sampling, and hence the example is not demonstrated correctly. This brief note presents a random angle sampling algorithm for the Buffon’s needle. We then compare the Buffon’s needle and Hit-and-Miss integration algorithms using Monte Carlo laboriousness comparison, and find that the Hit-and-Miss algorithm is superior.
基金National Key R&D Program of China-Science and Technology Innovation 2030-"New Generation of Artificial Intelligence"Major Project(2018AAA0102100)Postgraduate Research Innovation Project of Hunan Province(CX2018B465)2011 Digital Chinese Medicine Innovation Research Platform of Hunan Digital Chinese Medicine Collaborative Innovation Center。
文摘Objective This study examined the research status and development process of FU Qingzhu’s Obstetrics and Gynecology(Fu Qing Zhu Nv Ke,《傅青主女科》,FQZNK)in the past 40 years with bibliometrics and visual analysis.Methods Retrieved all related literature in the research field of FQZNK from the domestic and foreign databases:China National Knowledge Infrastructure(CNKI),China Science and Technology Journal Database(VIP),Wanfang Database,and Web of Science(WOS)core database,including Science Citation Index Expanded(SCIE),Social Sciences Citation Index(SSCI),and Arts&Humanities Citation Index(A&HCI).The search range was from January 1,1980 to March 10,2021.In addition,bibliometrics and CiteSpace 5.7.R2 software were used to analyze literature types,published journals,cited literature,the number of author publica-tions,co-author networks,co-institution networks,keyword co-occurrence networks,keyword clusters,and keyword bursts.Results A total of 678 valid records were included in the final dataset.Literature types,high publication journals,highly cited literature,high-yield institutions,high-yield research teams,and high-productivity scholars in this research field were found through bibliometrics.Liter-ature types can be divided into four categories,among which 451 are theoretical studies on academic thoughts of FQZNK,accounting for 66.5%of the included journals.The Journal of Shanxi Traditional Chinese Medicine had the largest volume of published articles(61),ac-counting for 9.0%of the total number of the included journals.The most cited literature was ZHOU Mingxin’s article“Using the quantitative method to discuss author’s authenticity and formula characteristics of FU Qingzhu’s Obstetrics and Gynecology”,which was cited 94 times.Hunan University of Chinese Medicine,the institution with the most publications,published 45 articles,and YOU Zhaoling,the most published author,published 33 articles.Moreover,it was found that most high-yield researchers came from high-yield institutions and that Hun-an University of Chinese Medicine had the most research on FQZNK.Keyword co-occur-rence analysis revealed that the keyword“FQZNK”had the highest frequency(597 times)and the highest centrality(1.00).Keyword cluster analysis used the Log-Likelihood Ratio(LLR)al-gorithm to form eleven important clusters:#0 treatment aiming at its root causes,#1 gynecopathy,#2 Siwu Decoction(四物汤),#3 FU Qingzhu,#4 post-partum,#5 infertility,#6 dysmenorrhea,#7 sterility,#8 coordinate the heart and kidney,#9 Danggui Buxue Decoction(当归补血汤),and#10 treatment.It was found that the prescriptions of FQZNK were studied mainly before 2000,the theoretical studies were mainly conducted before 2010,and its clinic-al application was mainly explored from 2010 until now.Diseases such as dysmenorrhea,morbid vaginal discharge,infertility,metrorrhagia,and polycystic ovarian syndrome(PCOS)have recently become popular topics in this field.Conclusion The current study provides more scientific,accurate,and comprehensive sci-entific support for further research and development of traditional Chinese medicine(TCM)in FQZNK.With this foundation,people can use burst detection to ascertain the current hot-spots in research,get their development trends,and forecast future research directions.In ad-dition,infertility,morbid vaginal discharge,flooding,and PCOS treatments based on TCM syndrome differentiation are currently popular research topics for FQZNK.
基金This work was supported by the Projects[NO.20154030 and NO.(2017)5655]from the Science and Technology Department of Guizhou Province and the National Natural Science Foundation of China(No.81873020).
文摘In this study,six kinetics models of indomethacin hydrophilic gel patch transdermal in vitro release was established,including zero-level,first-order,Higuchi-level,Ritger-Peppas,Weibull and Hixcon-Crowell dynamic equations.The chemical permeation enhancers,including 3%and 5%Azone,and iontophoresis were used as the control.Transdermal diffusion tests were performed in vitro and indomethacin was quantified by high performance liquid chromatography system.The transdermal parameter of the Higuchi and Weibull dynamic equations,indicated that Fu’s cupping therapy(FCT)could significantly improve Higuchi and Weibull kinetic parameters in vitro transdermal,increased transdermal rate and permeability coefficient,reduced lagging time.Additionally,statistical analysis speculated the skin barrier function could be restored after 46 h treatment.Hence,as a new physical transdermal drug delivery technology,transdermal permeation effects produced by FCT are obvious,which has the characteristics of traditional Chinese medicine and has important clinical application value.
文摘The great Chinese poet Du Fu＇s poetry in the Dang Dynasty has important significance in the development of the world culture. Confucianism is the main theme of his poetry, which penetrates connotation of benevolence, love of all life and self-cultivation. The famous poet Sun Dayu strived to highlight ideological content and the essence of Confucianism in Du Fu＇s poetry through translation. On the basis of deep understanding of the source text and careful choice of words, Sun Dayu mainly used literal translation, free translation and explanation in order to make the target readers know the spirit of Confucianism. His translation activities showed solicitude for national destiny and historical responsibility.
文摘In this paper, the background, evolution, basic meaning, clinical application and the detail operating procedures of the differential insertion depth in filiform needle acupuncture were discussed based on the classical expositions of the Yellow Emperor’s Canon of Medicine. It is believed that the differential insertion depth reflects the basic idea of expelling the evil Qi from the body in the application of traditional acupuncture. Since the site of evil invasion has different shades, the position of evil Qi and correct differentiation has become the operation key points of needle insertion. Apart from this, the Yellow Emperor’s Canon of Medicine has further associated the clinical application of filiform needle insertion depth with the seasonal change of Yin and Yang, the body built of the patients, the nature of the diseases, the heat or cold pathogenic factors of the illness, the excess and deficiency of the patient, and the reinforcing and reducing function of acupuncture. These elaborations have greatly enriched the basic content of acupuncture and laid a systematic theoretical foundation of filiform needle operation. The differential insertion depth in acupuncture has its specific meaning, the emphasis of insertion depth of filiform needle with its differentiated clinical implication exemplifies the perceptual thinking features of traditional acupuncture and typical reveals the uniqueness of Chinese civilization.
文摘Introduction: Triple immunohistochemical (IHC) stains including antibodies specific for alpha-methylacyl-CoA-racemase and basal cell markers have been a valuable aid in accurate identification of prostate carcinoma. However, accurate quantification of minuscule areas of prostate carcinoma in biopsy specimens can often be a challenge. Here we assessed the diagnostic value and quantitative use of automated digital image analysis on triple IHC stained prostate needle biopsies. Methods: Twelve cases of prostate needle biopsy material including 75 needle cores were stained with triple-antibody cocktail (P504S + 34βE12 + p63). Slides were digitally scanned with the APERIO digital image analyzer and evaluated with the GENIE pattern and color recognition digital image analysis that we developed. A slide with known areas of adenocarcinoma, high grade prostatic intraepithelial neoplasia (PIN), benign glands and stroma was used as a training set for the automated digital image analysis platform. Results: Among 75 needle biopsy cores, 19 (25.33%) contained adenocarcinoma by histology. Digital image analysis recognized adenocarcinoma in 95% of these needle biopsies. The average area of the needle biopsy was 7.63 mm2 and overall the average area of tumor was 0.196 mm2. The smallest area of tumor recognized by the program was 0.0022 mm2 (0.0363% of the core) and the largest was 0.62 mm2 (8.17% of the core) among needle core biopsies. False positives resulted from areas of high grade PIN with patchy basal cells. The false negative was caused by uneven AMACR staining in one area of adenocarcinoma. Digital recognition of areas of interest was improved by three successive image analysis training which increased the sensitivity and specificity from 83% and 89% to 90% and 93%, respectively. Conclusions: Digital image analysis in concert with IHC triple staining may be useful for accurate detection and quantitative analysis of small foci of prostatic adenocarcinoma. Defining methods to increase the sensitivity and specificity of quantitative automated digital image analysis will likely evolve as an area of investigation. Future automated digital scanning and innovative pattern and color recognition technologies may open avenues for classifying a variety of prostate lesions.
文摘Objective: To study the correlation of S100A13 and FOXA1 expression with cell cycle and cell invasion in fine needle aspiration thyroid carcinoma tissue. Methods: Patients who received ultrasound-guided thyroid nodule fine needle aspiration in Haiyang People's Hospital between April 2015 and February 2017 were selected, and the tissues were divided into malignant thyroid tissue and benign thyroid nodules according to the pathological results after biopsy. The expression of S100A13, FOXA1, cell cycle molecules and cell invasion molecules were measured. Results: S100A13, FOXA1, CDK2, CyclinD1, MCM2, MCM7, SKP2, CLOCK, STAT3, STAT5, N-cadherin, MT1-MMP and ADAM17 mRNA expression in thyroid carcinoma tissue were significantly higher than those in benign thyroid nodule;CDK2, CyclinD1, MCM2, MCM7, SKP2 and CLOCK mRNA expression in thyroid carcinoma tissue with high FOXA1 expression were significantly higher than those in thyroid carcinoma tissue with low FOXA1 expression;STAT3, STAT5, N-cadherin, MT1-MMP and ADAM17 mRNA expression in thyroid carcinoma tissue with high S100A13 expression were significantly higher than those in thyroid cancer tissue with low S100A13 expression. Conclusions: High expression of S100A13 and FOXA1 in thyroid carcinoma can promote cell invasion and cell cycle progression.
文摘目的观察浮针疗法治疗早中期膝骨关节炎对关节软骨厚度的影响。方法将60例早中期膝骨关节炎患者随机分为治疗组和对照组,每组30例。治疗组采用浮针治疗,对照组采用电针治疗。观察两组治疗前后疼痛视觉模拟量表(visual analog scale,VAS)评分、疼痛开始缓解时间、Lysholm膝关节功能评分、平衡功能及股骨内侧和外侧髁软骨厚度的变化。结果治疗组疼痛开始缓解时间短于对照组(P<0.05)。治疗后,两组VAS评分以及平衡功能的横向标准差和前后标准差均较治疗前降低(P<0.05),Lysholm膝关节功能评分、平衡功能的稳定极限均较治疗前升高(P<0.05)。治疗后3个月,两组VAS评分较治疗前降低(P<0.05),Lysholm膝关节功能评分较治疗前升高(P<0.05),膝关节内外侧髁软骨厚度均较治疗前增加(P<0.05)。治疗后和治疗后3个月,治疗组VAS评分均低于对照组(P<0.05),Lysholm膝关节功能评分均高于对照组(P<0.05)。治疗后,治疗组横向标准差低于对照组(P<0.05),稳定极限高于对照组(P<0.05)。结论浮针治疗早中期膝骨关节炎起效速度优于电针治疗,能有效缓解疼痛症状及提高膝关节功能,可改善软骨损伤。
