Objective:To investigate the effect of Fufei Gushen Decoction on airway inflammation and glucocorticoid receptor in rats with chronic obstructive pulmonary disease.Methods:Fifty Wistar male rats were randomly divided ...Objective:To investigate the effect of Fufei Gushen Decoction on airway inflammation and glucocorticoid receptor in rats with chronic obstructive pulmonary disease.Methods:Fifty Wistar male rats were randomly divided into 5 groups:blank control group,COPD model group,Fufei Gushen Yin high,medium and low dose groups,10 rats in each group,except the blank control group,the remaining 4 groups were Smoked combined with lipopolysaccharide(LPS),cold air stimulation to create CODP rat model.After successful modeling,the blank control group and COPD model group were fed with distilled water 3ml/only,Fufei Gushen Yin high,medium and low dose groups were given 1.02,0.51,0.26g Chinese medicine granules/100g/day,respectively.2 times a day for 28 consecutive days.Samples were collected,hematoxylin-eosin(HE)staining was used to observe the pathological changes of lung tissue,and enzyme-linked immunosorbent assay(ELISA)was used to detect the tumor necrosis factor alpha(TNF-α)in the serum and right alveolar lavage fluid(BALF)of rats in each group.),the content of transforming growth factorβ1(TGF-β1),interleukin-17(IL-17A)and matrix metalloproteinase(MMP-9)and tissue inhibitor of metalloproteinase-1(TIMP-1)in the left lung tissue The expression level of real-time fluorescence quantitative polymerase chain reaction(Real-time PCR)rat left lung tissue GRmRNA,immunohistochemistry(IHC)to determine the expression level of left lung tissue glucocorticoid receptor(GR).Results:The content of TNF-α,TGF-β1 and IL-17A in the serum of COPD rats in Fufei Gushen Yin high,medium and low dose groups and BALF were significantly reduced compared with the COPD model group(P<0.05);The expressions of TIMP-1 and MMP-9 in tissues were lower than those in COPD model group(P<0.05);the expressions of GRmRNA and GR in lung tissues were higher than those in COPD model group(P<0.05),and were higher in Fufei Gushen Yin Among the middle-and low-dose groups,the middle-dose group has the most significant effect.Conclusion:Fufei Gushen Decoction can inhibit the release of inflammatory factors in lung tissue of COPD rats,improve airway inflammation and remodeling,and increase hormone sensitivity.展开更多
目的探讨复肺方辅助TP方案(紫杉醇+顺铂)在中晚期非小细胞肺癌(Non-small-cell lung cancer,NSCLC)中的治疗价值。方法选取2017年1月—2020年8月期间张家港市中医医院收治的中晚期NSCLC患者90例,采用随机数字表法分对照组和试验组,每组...目的探讨复肺方辅助TP方案(紫杉醇+顺铂)在中晚期非小细胞肺癌(Non-small-cell lung cancer,NSCLC)中的治疗价值。方法选取2017年1月—2020年8月期间张家港市中医医院收治的中晚期NSCLC患者90例,采用随机数字表法分对照组和试验组,每组各45例。两组患者均给予TP化疗方案(完成4个化疗周期,历时12周),在此基础上,试验组自拟复肺方辅助治疗12周。治疗12周后,观察比较两组患者肿瘤疗效、中医证候疗效、治疗前后血清肿瘤标志物[细胞角蛋白19可溶性片段(Cytokeratin 19 soluble fragment,CYFRA21-1)、糖蛋白抗原125(Glycoprotein antigen 125,CA125)、癌胚抗原(Carcinoembryonic antigen,CEA)]水平及生活质量(European Organization for Research and Treatment of Cancer quality of life questionnaire core 30,EORTC QLQ-C30)评分变化、不良反应发生率,随访12个月,统计两组患者生存情况。结果(1)肿瘤疗效:治疗后试验组疾病控制率91.11%(41/45)高于对照组75.56%(34/45),差异有统计学意义(P<0.05);试验组治疗总有效率55.56%(25/45)高于对照组42.22%(19/45),差异有统计学意义(P<0.05);(2)证候疗效:治疗后试验组中医证候疗效总有效率88.89%(40/45)高于对照组57.78%(26/45),差异有统计学意义(P<0.05);(3)肿瘤标志物:治疗后两组患者肿瘤标志物均低于治疗前,差异有统计学意义(P<0.05);且试验组低于对照组,差异有统计学意义(P<0.05);(4)生活质量:治疗后两组患者总体健康状况领域、功能领域评分较治疗前升高,症状领域评分较治疗前降低,差异有统计学意义(P<0.05);且试验组总体健康状况领域、功能领域评分高于对照组,症状领域评分低于对照组,差异有统计学意义(P<0.05);(5)毒副作用:试验组血小板减少、白细胞减少、血清肌酐升高、丙氨酸氨基转移酶升高、便秘、口腔炎、周围神经异常发生率均低于对照组,差异有统计学意义(P<0.05);(6)生存情况:试验组中位生存时间较对照组长,且12个月生存率较对照组高,差异有统计学意义(P<0.05)。结论复肺方辅助TP方案治疗中晚期NSCLC效果明显,有利于提高疾病控制率,下调血清肿瘤标志物表达,改善患者生活质量,延长生命期限,且能够降低化疗不良反应发生率。展开更多
基金Construction Project for Famous Doctors of Traditional Chinese Medicine of Ruikang Hospital Affiliated to Guangxi University of Traditional Chinese Medicine(No.[2019]18)。
文摘Objective:To investigate the effect of Fufei Gushen Decoction on airway inflammation and glucocorticoid receptor in rats with chronic obstructive pulmonary disease.Methods:Fifty Wistar male rats were randomly divided into 5 groups:blank control group,COPD model group,Fufei Gushen Yin high,medium and low dose groups,10 rats in each group,except the blank control group,the remaining 4 groups were Smoked combined with lipopolysaccharide(LPS),cold air stimulation to create CODP rat model.After successful modeling,the blank control group and COPD model group were fed with distilled water 3ml/only,Fufei Gushen Yin high,medium and low dose groups were given 1.02,0.51,0.26g Chinese medicine granules/100g/day,respectively.2 times a day for 28 consecutive days.Samples were collected,hematoxylin-eosin(HE)staining was used to observe the pathological changes of lung tissue,and enzyme-linked immunosorbent assay(ELISA)was used to detect the tumor necrosis factor alpha(TNF-α)in the serum and right alveolar lavage fluid(BALF)of rats in each group.),the content of transforming growth factorβ1(TGF-β1),interleukin-17(IL-17A)and matrix metalloproteinase(MMP-9)and tissue inhibitor of metalloproteinase-1(TIMP-1)in the left lung tissue The expression level of real-time fluorescence quantitative polymerase chain reaction(Real-time PCR)rat left lung tissue GRmRNA,immunohistochemistry(IHC)to determine the expression level of left lung tissue glucocorticoid receptor(GR).Results:The content of TNF-α,TGF-β1 and IL-17A in the serum of COPD rats in Fufei Gushen Yin high,medium and low dose groups and BALF were significantly reduced compared with the COPD model group(P<0.05);The expressions of TIMP-1 and MMP-9 in tissues were lower than those in COPD model group(P<0.05);the expressions of GRmRNA and GR in lung tissues were higher than those in COPD model group(P<0.05),and were higher in Fufei Gushen Yin Among the middle-and low-dose groups,the middle-dose group has the most significant effect.Conclusion:Fufei Gushen Decoction can inhibit the release of inflammatory factors in lung tissue of COPD rats,improve airway inflammation and remodeling,and increase hormone sensitivity.
文摘目的探讨复肺方辅助TP方案(紫杉醇+顺铂)在中晚期非小细胞肺癌(Non-small-cell lung cancer,NSCLC)中的治疗价值。方法选取2017年1月—2020年8月期间张家港市中医医院收治的中晚期NSCLC患者90例,采用随机数字表法分对照组和试验组,每组各45例。两组患者均给予TP化疗方案(完成4个化疗周期,历时12周),在此基础上,试验组自拟复肺方辅助治疗12周。治疗12周后,观察比较两组患者肿瘤疗效、中医证候疗效、治疗前后血清肿瘤标志物[细胞角蛋白19可溶性片段(Cytokeratin 19 soluble fragment,CYFRA21-1)、糖蛋白抗原125(Glycoprotein antigen 125,CA125)、癌胚抗原(Carcinoembryonic antigen,CEA)]水平及生活质量(European Organization for Research and Treatment of Cancer quality of life questionnaire core 30,EORTC QLQ-C30)评分变化、不良反应发生率,随访12个月,统计两组患者生存情况。结果(1)肿瘤疗效:治疗后试验组疾病控制率91.11%(41/45)高于对照组75.56%(34/45),差异有统计学意义(P<0.05);试验组治疗总有效率55.56%(25/45)高于对照组42.22%(19/45),差异有统计学意义(P<0.05);(2)证候疗效:治疗后试验组中医证候疗效总有效率88.89%(40/45)高于对照组57.78%(26/45),差异有统计学意义(P<0.05);(3)肿瘤标志物:治疗后两组患者肿瘤标志物均低于治疗前,差异有统计学意义(P<0.05);且试验组低于对照组,差异有统计学意义(P<0.05);(4)生活质量:治疗后两组患者总体健康状况领域、功能领域评分较治疗前升高,症状领域评分较治疗前降低,差异有统计学意义(P<0.05);且试验组总体健康状况领域、功能领域评分高于对照组,症状领域评分低于对照组,差异有统计学意义(P<0.05);(5)毒副作用:试验组血小板减少、白细胞减少、血清肌酐升高、丙氨酸氨基转移酶升高、便秘、口腔炎、周围神经异常发生率均低于对照组,差异有统计学意义(P<0.05);(6)生存情况:试验组中位生存时间较对照组长,且12个月生存率较对照组高,差异有统计学意义(P<0.05)。结论复肺方辅助TP方案治疗中晚期NSCLC效果明显,有利于提高疾病控制率,下调血清肿瘤标志物表达,改善患者生活质量,延长生命期限,且能够降低化疗不良反应发生率。
