Crude plant extracts of ringworm cassia, Cassia alata L. and turmeric, Curcuma longa L. were prepared by either hot water or organic solvents such as ethanol and ether. Various concentrations of the crude extract were...Crude plant extracts of ringworm cassia, Cassia alata L. and turmeric, Curcuma longa L. were prepared by either hot water or organic solvents such as ethanol and ether. Various concentrations of the crude extract were then subjected to an in vitro test for their effectiveness on mycelia growth inhibition against some important plant pathogenic fungi such as Alternaria alternata, Colletotrichum gloeosporioides, Fusarium oxysporum fsp. lycopersici, Sclerotium rolfsii, Phytophthora infestans and Pythium sp. in comparison to commercial fungicides such as copper oxychloride and mancozeb. Reduction of the fungal growth was significantly obtained with C. longa extracts and the best median effective inhibitory concentration (IC50) value of 6.07, 6.50 and 7.13 mg/ml was from the ethanol extract for S. rolfsii, C. gloeosporioides and F. oxysporum fsp. lycopersici respectively. While all extracts from C. alata were almost the least effective against these fungi. The efficacy of C. longa extracts therefore, provided an alternative regime for the control of the fungal diseases and a promising appreciable choice for a replacement of chemical fungicides.展开更多
Pathogenic fungi cause dying off of apple tree and giving negative impact to farmers. The objective of this study was to identify tree pathogenic fungi isolates from apple tree in the Batu City and to know phylogeneti...Pathogenic fungi cause dying off of apple tree and giving negative impact to farmers. The objective of this study was to identify tree pathogenic fungi isolates from apple tree in the Batu City and to know phylogenetic relationship of the isolates based on the DNA sequences. The method in this study included subculture of three pathogenic fungi isolates (M1, M4, and MB1) using V8 Juice Agar medium for one to three days, continued with DNA isolation, amplification using ITS5/ITS4 primer, purification, sequencing, and sequence homology analysis of DNA amplicons of each isolates with reference isolates. The results showed similarity value of DNA sequence of the three isolates M1, M4, and MB1 of more than 99%. The three isolates M1, M4, and MB1 have a similarity value of DNA sequence with species of Pythium splendens of 99.84%, 99.67% and 99.83%, respectively. On the other hand similarity value between those isolates with Phytophthora was less than 76%. It was concluded that the three isolates are Py. splendens.展开更多
目的:外生菌根真菌松茸Tricholoma matsutake(S.Ito et Imai)Sing.又名松口蘑,是一种极其珍稀昂贵的野生食用蘑菇资源。为了保护利用松茸资源的生物多样性,需要研究发展适用松茸的基因组DNA的提取方法。方法:对传统的液氮冷冻研磨的细...目的:外生菌根真菌松茸Tricholoma matsutake(S.Ito et Imai)Sing.又名松口蘑,是一种极其珍稀昂贵的野生食用蘑菇资源。为了保护利用松茸资源的生物多样性,需要研究发展适用松茸的基因组DNA的提取方法。方法:对传统的液氮冷冻研磨的细胞破壁方法加以改进,采用液氮冷冻与室温融化交替处理松茸菌丝体与其它供试真菌细胞,进而萃取、浓缩、沉淀DNA。结果:利用本文研究的方法分离纯化出了满足分子生物学实验要求的高质量细胞总DNA。结论:该法相对简便、安全,可行、可靠,对供试真菌培养物需求量较少,既适用于担子真菌松茸,也适用于供试的其它真菌类群如酵母菌、丝状霉菌,应用前景广阔。展开更多
本研究旨在比较CTAB法、改良CTAB法、SDS法、氯化苄法和Chelex-100法提取真菌DNA作为PCR模板的效果,以生长速率、菌落形态差异较大的11个煤污病菌属真菌为研究对象,以核糖体RNA(r RNA)基因中的内转录间隔区(ITS)序列和28S r RNA基因部...本研究旨在比较CTAB法、改良CTAB法、SDS法、氯化苄法和Chelex-100法提取真菌DNA作为PCR模板的效果,以生长速率、菌落形态差异较大的11个煤污病菌属真菌为研究对象,以核糖体RNA(r RNA)基因中的内转录间隔区(ITS)序列和28S r RNA基因部分序列的扩增率为指标,采用SPSS软件对结果进行方差分析。结果表明,CTAB法和改良CTAB法适用真菌范围最广,分别有7个和9个属真菌的扩增率都大于70%且无显著性差异;氯化苄法和Chelex-100法适用范围居中,分别有6个和8个属真菌的扩增率较高且无显著性差异,扩增率分别大于70%和50%;SDS法适用范围最窄,有6个属真菌的扩增率高于50%且无显著性差异。大多数煤污病菌至少有2种及以上的DNA提取方法能够取得较好的效果,但链丝孢属真菌只有用氯化苄法提取DNA效果最好。采用改良CTAB法结合氯化苄法,能够满足所有供试煤污病菌类群真菌提取基因组DNA用作PCR反应模板的需要。展开更多
文摘Crude plant extracts of ringworm cassia, Cassia alata L. and turmeric, Curcuma longa L. were prepared by either hot water or organic solvents such as ethanol and ether. Various concentrations of the crude extract were then subjected to an in vitro test for their effectiveness on mycelia growth inhibition against some important plant pathogenic fungi such as Alternaria alternata, Colletotrichum gloeosporioides, Fusarium oxysporum fsp. lycopersici, Sclerotium rolfsii, Phytophthora infestans and Pythium sp. in comparison to commercial fungicides such as copper oxychloride and mancozeb. Reduction of the fungal growth was significantly obtained with C. longa extracts and the best median effective inhibitory concentration (IC50) value of 6.07, 6.50 and 7.13 mg/ml was from the ethanol extract for S. rolfsii, C. gloeosporioides and F. oxysporum fsp. lycopersici respectively. While all extracts from C. alata were almost the least effective against these fungi. The efficacy of C. longa extracts therefore, provided an alternative regime for the control of the fungal diseases and a promising appreciable choice for a replacement of chemical fungicides.
文摘Pathogenic fungi cause dying off of apple tree and giving negative impact to farmers. The objective of this study was to identify tree pathogenic fungi isolates from apple tree in the Batu City and to know phylogenetic relationship of the isolates based on the DNA sequences. The method in this study included subculture of three pathogenic fungi isolates (M1, M4, and MB1) using V8 Juice Agar medium for one to three days, continued with DNA isolation, amplification using ITS5/ITS4 primer, purification, sequencing, and sequence homology analysis of DNA amplicons of each isolates with reference isolates. The results showed similarity value of DNA sequence of the three isolates M1, M4, and MB1 of more than 99%. The three isolates M1, M4, and MB1 have a similarity value of DNA sequence with species of Pythium splendens of 99.84%, 99.67% and 99.83%, respectively. On the other hand similarity value between those isolates with Phytophthora was less than 76%. It was concluded that the three isolates are Py. splendens.
文摘目的:外生菌根真菌松茸Tricholoma matsutake(S.Ito et Imai)Sing.又名松口蘑,是一种极其珍稀昂贵的野生食用蘑菇资源。为了保护利用松茸资源的生物多样性,需要研究发展适用松茸的基因组DNA的提取方法。方法:对传统的液氮冷冻研磨的细胞破壁方法加以改进,采用液氮冷冻与室温融化交替处理松茸菌丝体与其它供试真菌细胞,进而萃取、浓缩、沉淀DNA。结果:利用本文研究的方法分离纯化出了满足分子生物学实验要求的高质量细胞总DNA。结论:该法相对简便、安全,可行、可靠,对供试真菌培养物需求量较少,既适用于担子真菌松茸,也适用于供试的其它真菌类群如酵母菌、丝状霉菌,应用前景广阔。
文摘本研究旨在比较CTAB法、改良CTAB法、SDS法、氯化苄法和Chelex-100法提取真菌DNA作为PCR模板的效果,以生长速率、菌落形态差异较大的11个煤污病菌属真菌为研究对象,以核糖体RNA(r RNA)基因中的内转录间隔区(ITS)序列和28S r RNA基因部分序列的扩增率为指标,采用SPSS软件对结果进行方差分析。结果表明,CTAB法和改良CTAB法适用真菌范围最广,分别有7个和9个属真菌的扩增率都大于70%且无显著性差异;氯化苄法和Chelex-100法适用范围居中,分别有6个和8个属真菌的扩增率较高且无显著性差异,扩增率分别大于70%和50%;SDS法适用范围最窄,有6个属真菌的扩增率高于50%且无显著性差异。大多数煤污病菌至少有2种及以上的DNA提取方法能够取得较好的效果,但链丝孢属真菌只有用氯化苄法提取DNA效果最好。采用改良CTAB法结合氯化苄法,能够满足所有供试煤污病菌类群真菌提取基因组DNA用作PCR反应模板的需要。
