The essay studies the influence of the different intensities of fishing stress on body weight （7.30 ± 1.48） g of red crucian carp juvenile body length, weight, fatness, feeding rate, specific growth rate, food ...The essay studies the influence of the different intensities of fishing stress on body weight （7.30 ± 1.48） g of red crucian carp juvenile body length, weight, fatness, feeding rate, specific growth rate, food conversion rate, intestinal protease, amylase and immunoglobulin. The result showed that body length, weight, fatness, feeding rate, specific growth rate of red crucian carp juvenile were significantly inhibited from fishing stress compared with the control group, and the magnitude of the inhibitory effect increased with the time going and increased. Fishing stress could increase the food conversion rate of red crucian carp juvenile, and the different intensity stress had no significant different influence. The intestinal protease activity decreased in the beginning, then increased, and was consistent with the level of the control group ultimately. High-stress group accelerated the decline of intestinal amylase activity of the juvenile. The stress increased the number of immunoglobulin of juvenile red crucian carp significantly, but the increase disappeard with the stress time extended.展开更多
In this study, we conducted a cross of white crucian carp （♀）xred crucian carp （♂） （WR）, and characterized the morphology, reproduction and genetics of the progeny. Different from parents, WR with the gray col...In this study, we conducted a cross of white crucian carp （♀）xred crucian carp （♂） （WR）, and characterized the morphology, reproduction and genetics of the progeny. Different from parents, WR with the gray color showed the hybrid morphological traits of both parents. WR possessed normal gonads producing mature eggs or sperm, and exhibited high fertilization rate （90.2%） and high hatchery rate （80.5%）, which contributed to produce and enlarge the population. WR with the same DNA content as parents was a diploid fish with 100 chromosomes （2n=100）. Amplified ITS of 45S rDNA, in WR the sequences consisting of 884 bp bases of the entire ITS-1 region, 5.8 S region, and entire ITS-2 region. The sequences showed high similarity between WR and its parents and leaned towards male inheritance. In WR, NTS of 5S rDNA consisted of three length types with total 654 bp bases. From sequence analysis of NTS, WR shared 94.2% and 95.1% similarities with their female and male parent, respectively. Sequence analysis of ITS and NTS revealed that there existed recombination and variation in the hybrid progeny, which was the genetic base for adaptation and speciation. In conclusion, we obtained WR from hybridization and it exhibited hybrid traits in morphology and variation in genetic composition showing essential difference with its parents. The obtainment of WR has important significance in fish genetic breeding.展开更多
Triploid carp(100%)with 150(3n=150)chromosomes were obtained by crossing the females of improved tetraploid hybrids(♀, 4n=200)of red crucian carp(♀)×common carp(♂)with the males of diploid yellow river carp(♂...Triploid carp(100%)with 150(3n=150)chromosomes were obtained by crossing the females of improved tetraploid hybrids(♀, 4n=200)of red crucian carp(♀)×common carp(♂)with the males of diploid yellow river carp(♂,2n=100).The crosses yielded transgenic triploid carp(positive triploid fish,44.2%of the progeny)and non-transgenic triploid carp(negative triploid fish). Histological examination of the gonads of 24-month-old positive triploid fish suggested they were sterile and the fish were not able to produce mature gametes during the breeding season.Morphologically,both the positive and negative triploid fish were similar.They had a spindle-shaped,laterally compressed,steel grey body with two pairs of barbells.Most of the quantifiable traits of the triploid carp were intermediate between those of the two parents.The positive and negative triploid fish were raised in the same pond for 2 years.The mean body weight of the positive triploid fish was 2.3 times higher than the negative triploid fish.The weight of the largest positive triploid fish was 2.91 times higher than that of the largest negative triploid fish.Thus,we produced fast-growing transgenic triploid carp that have a reduced ecological risk because of their inability to mate and produce progeny.展开更多
采用 DNA 杂交的方法,对异育银鲫及其人工杂合种进行了外源 DNA 的检测分析,发现两个雌核发育系的异育银鲫及其人工杂合种的 DNA 与红鲤 DNA 片段间的分子杂交均有阳性斑点出现,这表明,在异育银鲫及其人工杂合种产生过程中,父本(红鲤)的...采用 DNA 杂交的方法,对异育银鲫及其人工杂合种进行了外源 DNA 的检测分析,发现两个雌核发育系的异育银鲫及其人工杂合种的 DNA 与红鲤 DNA 片段间的分子杂交均有阳性斑点出现,这表明,在异育银鲫及其人工杂合种产生过程中,父本(红鲤)的 DNA 片段的确可以随机地掺入到母本细胞的 DNA 中,从而产生了异精效应,使其子代生长加快,并出现父本性状。展开更多
文摘The essay studies the influence of the different intensities of fishing stress on body weight （7.30 ± 1.48） g of red crucian carp juvenile body length, weight, fatness, feeding rate, specific growth rate, food conversion rate, intestinal protease, amylase and immunoglobulin. The result showed that body length, weight, fatness, feeding rate, specific growth rate of red crucian carp juvenile were significantly inhibited from fishing stress compared with the control group, and the magnitude of the inhibitory effect increased with the time going and increased. Fishing stress could increase the food conversion rate of red crucian carp juvenile, and the different intensity stress had no significant different influence. The intestinal protease activity decreased in the beginning, then increased, and was consistent with the level of the control group ultimately. High-stress group accelerated the decline of intestinal amylase activity of the juvenile. The stress increased the number of immunoglobulin of juvenile red crucian carp significantly, but the increase disappeard with the stress time extended.
基金supported by Major International Cooperation Projects of the National Natural Science Foundation of China(31210103918)the National Natural Science Foundation of China(31201980,31430088 and 31272651)+5 种基金the National High Technology Research and Development Program of China(2011AA100403)the Natural Science Foundation of Hunan Province,China(14JJ3072)Science-Technology Foundation of Hunan Province,China(2014FJ3084)Research Foundation of Education Bureau of Hunan Province,China(13C523)the Cooperative Innovation Center of Engineering and New Products for Developmental Biology of Hunan Province(20134486)the Construct Program of the Key Discipline in Hunan Province,and Construct Program of the National Key Discipline
文摘In this study, we conducted a cross of white crucian carp （♀）xred crucian carp （♂） （WR）, and characterized the morphology, reproduction and genetics of the progeny. Different from parents, WR with the gray color showed the hybrid morphological traits of both parents. WR possessed normal gonads producing mature eggs or sperm, and exhibited high fertilization rate （90.2%） and high hatchery rate （80.5%）, which contributed to produce and enlarge the population. WR with the same DNA content as parents was a diploid fish with 100 chromosomes （2n=100）. Amplified ITS of 45S rDNA, in WR the sequences consisting of 884 bp bases of the entire ITS-1 region, 5.8 S region, and entire ITS-2 region. The sequences showed high similarity between WR and its parents and leaned towards male inheritance. In WR, NTS of 5S rDNA consisted of three length types with total 654 bp bases. From sequence analysis of NTS, WR shared 94.2% and 95.1% similarities with their female and male parent, respectively. Sequence analysis of ITS and NTS revealed that there existed recombination and variation in the hybrid progeny, which was the genetic base for adaptation and speciation. In conclusion, we obtained WR from hybridization and it exhibited hybrid traits in morphology and variation in genetic composition showing essential difference with its parents. The obtainment of WR has important significance in fish genetic breeding.
基金supported by the National Key Basic Research Program of China (2007CB109206)the National Special Fund for Research in Public Welfare Sector (200903046-08)
文摘Triploid carp(100%)with 150(3n=150)chromosomes were obtained by crossing the females of improved tetraploid hybrids(♀, 4n=200)of red crucian carp(♀)×common carp(♂)with the males of diploid yellow river carp(♂,2n=100).The crosses yielded transgenic triploid carp(positive triploid fish,44.2%of the progeny)and non-transgenic triploid carp(negative triploid fish). Histological examination of the gonads of 24-month-old positive triploid fish suggested they were sterile and the fish were not able to produce mature gametes during the breeding season.Morphologically,both the positive and negative triploid fish were similar.They had a spindle-shaped,laterally compressed,steel grey body with two pairs of barbells.Most of the quantifiable traits of the triploid carp were intermediate between those of the two parents.The positive and negative triploid fish were raised in the same pond for 2 years.The mean body weight of the positive triploid fish was 2.3 times higher than the negative triploid fish.The weight of the largest positive triploid fish was 2.91 times higher than that of the largest negative triploid fish.Thus,we produced fast-growing transgenic triploid carp that have a reduced ecological risk because of their inability to mate and produce progeny.
文摘采用 DNA 杂交的方法,对异育银鲫及其人工杂合种进行了外源 DNA 的检测分析,发现两个雌核发育系的异育银鲫及其人工杂合种的 DNA 与红鲤 DNA 片段间的分子杂交均有阳性斑点出现,这表明,在异育银鲫及其人工杂合种产生过程中,父本(红鲤)的 DNA 片段的确可以随机地掺入到母本细胞的 DNA 中,从而产生了异精效应,使其子代生长加快,并出现父本性状。
