A New Furostanol Glycoside from Polygonatum odoratum

A new furostanol component glycosylated only at C-26 was isolated from the rhizomes of Polygonatum odoratum (Mill.) Druce, and its structure was characterized as 22-hydroxy-25(R and S) furost-5-en-12-on-3b, 22, 26-triol 26-O-b-D-glucopyranoside on the basis of spectroscopic techniques and chemical methods.

Two New Furostanol Glycosides from Asparagus cochinchinensis

Two new furostanol oligoglycosides named as aspacochioside A (1) and B (2) were isolated from the roots of Asparagus cochinchinensis (Lour.) Merr.. Their structures were elucidated to be 3-O-[{a-L-rhamnopyranosyl-(14)}{b-D-glucopyranosyl}]-26-O-[b-D-glucopy- ranosyl]-(25S)-5b-furostane-3b,22a,26-triol 1 and 3-O-[{a-L-rhamnopyranosyl-(14)}{b-D-glu- copyranosyl}]-26-O-[b-D-glucopyranosyl]-22a-methoxy-(25S)-5b-furostane-3b,26-diol 2 on the basis of spectroscopic techniques and chemical methods.

Furostanol saponins with inhibitory action against COX-2 production from Tupistra chinensis rhizomes

Two furostanol saponins were obtained from the n-butanol fraction of methanol extract from Tupistra chinensis rhizomes, a folk medicine of Shennongjia Forest District of Hubei Province. Their structures were determined as （25S）-26-O-（β-D-glucopyranosyl）- furost-1β, 3β, 22α, 26-tetrol-3-O-β-D-glucopyranosyl-（1 → 4）-β-D-glucopyranosyl-（1 → 2）-β-D-glucopyranoside （1） and （25R）- 26-O-（β-D-glucopyranosyl）-furost-1β, 3β 22a, 26-tetrol 3-O-β-D-glucopyranosyl-（1 → 4）-β-D-glucopyranosyl-（1 → 2）-β-D-glu- copyranoside （2）, on basis of chemical and spectroscopic evidences. 1 and 2 displayed marked inhibitory action towards COX-2 production in macrophages of the rat abdomen induced by LPS at 20 μg/mL.

A Furostanol Saponin from the Cytotoxic Fraction of Tupistra chinensis Rhizomes

A furostanol saponin was obtained from the n-butanol fraction of methanol extract from Tupistra chinensis rhizomes, a folk medicine of Shennongjia Forest District of Hubei Province. Its structure was determined as 3-O-β-D-glucopyranosyl-（25S）-22-O-methyl-5β-furost-1β, 3β, 5β, 22α; 26-pentaol-26-O-β-D-glucopyranoside （1） on the basis of chemical and spectroscopic evidences. The n-butanol fraction displayed marked inhibitory activity in vitro towards HeLa and HL-60 human tumor cell lines by MTT method.

A New Furostanol Saponin from Dioscorea futshauensis

A new furostanol saponin presenting moderate bioactivity of inducing morphological deformation of Pyricularia oryzae mycelia was isolated from Dioscorea futshauensis R.Kunth by bioactivity-guided fractionation. The structure was established as 26-O-b-D-glucopyranosyl-3b, 26-diol-23(S)-methoxyl-(25R)-furost-5,20 (22)-diene-3-O-[a-L-rhamnopyranosyl (12)-b-D- glucopyranosyl (13)]-b-D-glucopyranoside on the basis of chemical evidencesand spectral analysis, especially by 2D-NMR techniques.

Two New Furostanol Saponins from Tribulus Terrestris L.

Two new furostanol glucosides, named tribufurosides G and H, were isolated from Tribulus terrestris L. The structures of the two new furostanol glucosides were elucidated as 26-O-β-D-glucopyranosyl-（25R）-5α-furost12-one-3β,22α,26-tetraol-3-O-β-D-glucopyranosyl-（ 1→2 ）-β-D-glucopyranosyl-（ 1→4 ）-β-D-galactopyranoside（ 1 ） and 26-O-β-D-glucopyranosyl-（25S）-5 α- 12-one-2 α,3,8,22 α,26-tetraol-3 -O-β-D-glucopyranosyl-（ 1 → 2 ）-β-D-glucopyranosyl-（1→4）-β-D-galactopyranoside（2） by 1 D, 2D NMR techniques, ESI-MS analysis as well as chemical methods.

STUDIES ON TWO NEW FUROSTANOL GLYCOSIDES FROM ALLIUM MACROSTEMON BUNGE

Two new furostanol saponins,named macrostemonoside E(1)and F(2),were isolated from the bulbs of Allium macrostemon Bunge (Liliaceae),their structures were established oy spectroscopic analysis and chemical evidences.Both possessed strong inhibitory activity on human platelet aggregation.

Two new furostanol saponins from the seeds of Allium cepa L.

从瞀或蒜之类 cepa L 的种子的 n-BuOH 摘录的 Phytochemical 分析。导致了四 furostanol saponins，其二是新混合物，命名 ceparoside A (1a ) 和 ceparoside B (2a ) 的隔离。新混合物的化学结构通过 NMR 的联合被阐明， MS 光谱数据和化学分析。

New steroidal alkaloid and furostanol glycosides isolated from Solanum lyratum with cytotoxicity

Two previously undescribed steroidal compounds, 16, 23-epoxy-22, 26-epimino-cholest-22(N), 23, 25(26)-trien-3β-ol-3-O-β-D-glucopyranosyl-(1→2)-β-D-glucopyranosyl-(1→4)-β-D-galactopyranoside(1) and 26-O-β-D-glucopyranosyl-(25 R)-5α-furost-20(22)-en-3β, 26-diol(2), together with 7 known ones including 26-O-β-D-glucopyranosyl-(25 R)-5, 20(22)-dien-furost-3β, 26-diol(3),(25 R)-5-en-spirost-3β-ol-O-β-D-glucopyranosyl-(1→4)-[α-L-rhmanopyranosyl-(1→2)]-β-D-galactopyranoside(4), funkioside D(5), aspidistrin(6), tigogenin-3-O-β-D-lucotrioside(7), desglucolanatigonin II(8), and degalactotigonin(9), were isolated from Solanum lyratum Thunb. Their cytotoxic activities were tested in two cancer cell lines by MTT method. One of the steroidal glycosides(6) showed significant cytotoxic activity against gastric cancer SGC7901 and liver cancer BEL-7402 cells.

Five new polyhydroxylated furostanol saponins from the rhizomes of Tupistra chinensis

Five new polyhydroxylated furostanol saponins were isolated from the roots and rhizomes of Tupistra chinensis,and their structures were determined as tupistrosides J–N(1–5),together with four known furostanol saponins(6–9),on the basis of physico-chemical properties and spectral analysis.Among them,compounds 3 and 5 showed cytotoxicity against human cancer cell lines SW620 with IC50 values of 72.5±2.4 and 77.3±2.5μmol·L^–1,respectively.Compound 4 showed cytotoxicity against human cancer cell line HepG2 with IC50 value of 88.6±2.1μmol·L^–1.

响应面法优选蒺藜炮制工艺

响应面法优选蒺藜最佳炮制工艺。采用紫外可见分光光度法,以呋甾皂苷含量为考察指标,选取影响总呋甾皂苷含量的主要因素烘制温度、烘制时间,进行2因素5水平的中心组合试验设计,优选蒺藜最佳炮制工艺。通过Design-Expert 12.0.3.0软件分析预测得出蒺藜最佳炮制工艺为烘制温度190.1℃、烘制时间10.51 min,在此条件下得到的呋甾皂苷含量为1.85%。优选的蒺藜炮制工艺稳定可行。

知母中呋甾皂甙的研究

自知母ＡｎｅｍａｒｒｈｅｎａａｓｐｈｏｄｅｌｏｉｄｅｓＢｇｅ．根茎的乙醇提取物中，经硅胶柱层析和制备型ＨＰＬＣ分得四种呋甾皂甙。用化学反应和波谱（ＩＲ，ＦＡＢ－ＭＳ，ＥＩ－ＭＳ，１ＨＮＭＲ，１３ＣＮＭＲ，ＤＥＰＴ，一维多重接力ＣｏＳＹ，二维接力ＨＯＨＡＨＡ，１Ｈ－１ＨＣＯＳＹ，１Ｈ－１３ＣＣＯＳＹ和ＮＯＥ差谱）解析，确定其结构为知母皂贰Ｂ（ａｎｅｍａｒｓａｐｏｎｉｎＢ，Ｉ），（２５Ｓ）－２６－Ｏ－β－Ｄ－吡喃葡萄糖基－５β－呋甾－２０（２２）－烯－３β，２６－二醇－３－Ｏ－β－Ｄ－吡喃葡萄糖基（１→２）－β－Ｄ－吡喃哺葡萄糖甙（Ⅱ），（２５Ｓ）－２６－Ｏ－β－Ｄ－吡喃葡萄糖基－２２－羟基－５β－呋甾－３β，２６－二醉－３－Ｏ－β－Ｄ－吡喃葡萄糖基（１→２）－β－Ｄ－吡喃半乳糖甙（Ⅲ），（２５Ｓ）－２６－Ｏ－β－Ｄ－吡喃葡萄糖基－２２－甲氧基－５β－呋甾－３β，２６－二醇－３－Ｏ－β－Ｄ－吡喃葡萄糖基（１→２）－β－Ｄ－吡喃半乳糖甙（Ⅳ）。Ⅱ为一新的呋甾皂甙，命名为知母皂甙Ｃ；化合物Ⅳ为首次从知母中分离得到，命名为知母皂甙Ｅ。初步的药理实验显示，化合物１～Ⅳ均有一定的清除羟自由基的作用。

薤白皂苷化合物对CD40L表达及血小板中性粒细胞黏附的影响

目的探讨薤白皂苷化合物对血小板表面CD40L表达及血小板中性粒细胞黏附的影响。方法制备富含血小板血浆和中性粒细胞,用荧光实时定量PCR和玫瑰花结计数的方法检测经不同浓度薤白化合物单体预处理后血小板表面CD40L的表达及其与中性粒细胞之间的黏附性。结果在6个薤白皂苷单体化合物中,化合物1、4、6呈剂量依赖性抑制血小板CD40L的表达,并明显抑制ADP诱导的血小板与中性粒细胞之间的黏附。结论薤白皂苷化合物可能具有抗血小板相关炎症的作用。

知母中的两种新呋甾皂苷

目的研究知母根茎的化学成分。方法采用水煎提取、大孔吸附树脂SP825柱色谱、反相C18柱色谱等进行分离,并通过化学手段和光谱分析(FAB-MS,1HNMR,13CNMR,1H-1HCOSY)鉴定其化学结构。结果从知母根茎中分离得到6种甾体皂苷,分别鉴定为:(25S)-26-O-β-D-吡喃葡糖基-22-羟基-5β-呋甾-2β,3β,26-三醇-3-O-β-D-吡喃葡糖基-(1→2)-β-D-吡喃半乳糖苷(知母皂苷N,1),知母皂苷E1(2),(25S)-26-O-β-D-吡喃葡糖基-22-甲氧基-5β-呋甾-2β,3β,26-三醇-3-O-β-D-吡喃葡糖基-(1→2)-β-D-吡喃半乳糖苷(知母皂苷O,3),知母皂苷E2(4),(25R)-26-O-β-D-吡喃葡糖基-22-羟基-5α-呋甾-2α,3β,26-三醇-3-O-β-D-吡喃葡糖基-(1→2)-[β-D-吡喃木糖基-(1→3)]-β-D-吡喃葡糖基-(1→4)-β-D-吡喃半乳糖苷(purpureagitosid,5),marcogenin-3-O-β-D-glucopyranosyl-(1→2)-β-D-galactopyranoside(6)。结论化合物1和3为新化合物,命名为知母皂苷N和知母皂苷O,化合物5为首次从知母中分离得到。

薤白中呋甾皂苷的含量测定

目的 :建立了测定薤白中呋甾皂苷含量的E试剂比色法。方法 :用对二甲氨基苯甲醛 HCl乙醇液的发色体系 ,测定波长 5 15nm ,利用均匀设计原理确定最佳实验条件。结果 :标准曲线在 0 .1975～ 0 .9880mg·ml-1线性关系良好 ,平均回收率为 97.7% (RSD =3.6 5 % )。结论 :此法可作为评价薤白的质量标准。

呋喃甾烷型皂甙的研究进展

综述了呋喃甾烷型皂甙(双糖链甾体皂甙)的结构特点、化学性质、生理活性和生源合成。列举了1966～1999年发现的双糖链甾体皂甙,共214个。

薤白中两种新呋甾皂甙的结构

自中药薤白(Allium macrostemon Bunge)鳞茎中分得三种甾体皂甙。经过化学降解和光谱(UV、IR,MS,~1H-NMR,^(13)C-NMR和FAB-MS)分析,鉴定其结构分别为(25R)-26-O-β-D-吡喃葡萄糖-5α-呋甾-20(22)-烯-3β,26-二醇-3-O-β-D-吡喃葡萄糖基(1→2)[β-D-吡喃葡萄糖基(1→3)]-β-D-吡喃葡萄精基(1→4)-β-D-吡喃半乳糖甙(Ⅰ),(25R)-26-O-β-D-吡喃葡萄糖基-5β-呋甾-20(22)-烯-3β,26-二醇-3-P-β-D-吡喃葡萄糖基(1→2)-β-D-吡喃半乳糖甙(Ⅱ)和异菝契皂甙元-3-O-D-吡喃葡萄糖基(1→2)-β-D-吡喃半乳糖甙(Ⅵ)。Ⅰ和Ⅱ为新的呋甾皂甙,分别命名为薤白甙戊和莲白甙己,初步药理实验测试二青均有较强的抑制ADP诱导的人血小板聚集作用。

天冬化学成分的研究(Ⅱ)

目的：研究天冬的化学成分。方法：经大孔树脂、反复硅胶柱色谱、Sephadex LH-20凝胶柱色谱和C-18反相硅胶柱色谱等各种色谱方法分离天冬中的化学成分，通过理化性质和现代波谱技术（MS、^1HNMR,^13CNMR）确定所分离化合物的结构。结果：从其乙醇提取物中分离得到5个化合物，经鉴定分别为薯蓣皂苷元-3-O-β-D-吡喃葡萄糖苷（Ⅰ），异菝葜皂苷元（Ⅱ），26-O-β-D-吡喃葡萄糖基-呋甾-3β，22，26-三醇-3-O-β—D-吡喃葡萄糖基（1→2）-O-β—D吡喃葡萄糖苷（Ⅲ），26-O-β-D-吡喃葡萄糖基-呋甾-5-烯-3β，2α，26-三醇-3-O-[α—α-吡喃鼠李糖基（1→2）]-[α-α-吡喃鼠李糖基-（1→4）]-β—D-吡喃葡萄糖苷（Ⅳ），26-O-β-D-吡喃葡萄糖基-呋甾-3β，26-二醇-22-甲氧基-3-O-α-L-吡喃鼠李糖基（1—4）-O-β-D-吡喃葡萄糖苷（Ⅴ）。结论：化合物Ⅰ～Ⅴ均为首次从天冬中得到。