In our previous studies, we identified 3 Trichoderma strains with anti-Fusarium oxysporum activity, including T. asperellum 525, T. harzianum 610, and T. pseudokoningii 886. Here, we evaluated the effects of these 3 T...In our previous studies, we identified 3 Trichoderma strains with anti-Fusarium oxysporum activity, including T. asperellum 525, T. harzianum 610, and T. pseudokoningii 886. Here, we evaluated the effects of these 3 Trichoderma strains on preventing cucumber fusarium wilt through pot culture and greenhouse culture experiments. All 3 Trichoderma strains demonstrated higher control effects toward cucumber fusarium wilt than previous studies, with efficacies over 78%. Additionally, inoculation with the 3 Trichoderma strains significantly promoted the quality and yield of cucumbers. Among the 3 strains, Trichoderma 866 was the most effective, with disease control efficacy of 78.64% and a cucumber yield increase of 33%. Furthermore, seedlings inoculated with Trichoderma exhibited significantly increased measures of plant height, stem diameter, leaf area, aboveground fresh weight, underground fresh weight, chlorophyll content, and nitric nitrogen content, as well as the activities of several stress-resistance enzymes, including superoxide dismutase(SOD), peroxidase(POD), catalase(CAT), polyphenol oxidase(PPO), and ascorbate oxidase(AAO). In addition, the plants inoculated with Trichoderma showed decreased cell membrane permeability and malondialdehyde(MDA) content in the leaves. Together, our results suggest that T. asperellum 525, T. harzianum 610, and T. pseudokoningii 886 inoculations inhibit F. oxysporum infection, stimulate the metabolism in cucumbers, and enhance the activities of stress-resistance enzymes, which consequently promote the growth of cucumber plants, prevent cucumber fusarium wilt, and improve the yield and quality of cucumbers. T. harzianum is a commonly used biocontrol fungus, while few studies have focused on T. asperellum or T. koningense. In this study, strains of T. asperellum and T. pseudokoningii showed excellent plant disease prevention and growth promoting effects on cucumber, indicating that they also have great potential as biocontrol fungi.展开更多
The amount of phenol compounds in the soil increased after adding organic material into the soil. It was found that p-hydroxybenzoic acid, p-coumaric acid and frulic acid alleviated Fusarium wilt of cucumber, the alle...The amount of phenol compounds in the soil increased after adding organic material into the soil. It was found that p-hydroxybenzoic acid, p-coumaric acid and frulic acid alleviated Fusarium wilt of cucumber, the alleviating effect of p-hydroxybenzoic acid was the best, followed by p-coumaric acid and frulic acid. The total amount of bacterial, actinomyces and fungus in high phenol compounds treatment decreased than that of control treatment, while the microorganisms' amount in low phenol compounds treatment increased. Phenol compounds inhibit the growth of pathogen.展开更多
Fusarium wilt caused by Fusarium oxysporum f.sp. cucumerinum (Owen) is one of the most devastating diseases in cucumber production worldwide. Recent progresses in the mechanism of resistance to Fusarium wilt in cucumb...Fusarium wilt caused by Fusarium oxysporum f.sp. cucumerinum (Owen) is one of the most devastating diseases in cucumber production worldwide. Recent progresses in the mechanism of resistance to Fusarium wilt in cucumber were reviewed in this paper, including pathogenic mechanism of Fusarium oxysporum, the resistance mechanism of cucumber, the heredity of resistance, and the location of resistance genes. Following works should be the location and cloning of resistance genes with molecular biologic methods.展开更多
With conventional fertilization as the control,the control effects of biological fertilizer and general organic fertilizer on watermelon Fusarium wilt were studied in the paper. The results showed that applying biolog...With conventional fertilization as the control,the control effects of biological fertilizer and general organic fertilizer on watermelon Fusarium wilt were studied in the paper. The results showed that applying biological fertilizer could effectively reduce the incidence rate of watermelon Fusarium wilt and significantly improve the quality of watermelon,while application of general organic fertilizer had no obvious control effect on watermelon Fusarium wilt.展开更多
Sugar Baby susceptible to Fusarium oxysporum f. sp. niveum was crossed with high resistant male parents D3 - 1 and D3 - 2, respectively. F1 hybrids showed high resistance. The segregation ratios of resistance to susce...Sugar Baby susceptible to Fusarium oxysporum f. sp. niveum was crossed with high resistant male parents D3 - 1 and D3 - 2, respectively. F1 hybrids showed high resistance. The segregation ratios of resistance to susceptibility of F2 and of BC1 hybrid population from Sugar Baby tallied with 3: 1 and 1: 1, respectively. The results indicated that the resistance to Fusarium wilt was a kind of dominant inheritance controlled by mono- gene or mono- segment DNA. Furthermore, 5 hybrid combinations with fine character were bred. Among them, 3 were high resistant to Fusarium wilt,and 2 were medium res展开更多
基金support from the National Key R&D Program of China (2018YFD0201202)the National Science and Technology Basic Work, China (2014FY120900)the 948 Program of China (2011-G4)
文摘In our previous studies, we identified 3 Trichoderma strains with anti-Fusarium oxysporum activity, including T. asperellum 525, T. harzianum 610, and T. pseudokoningii 886. Here, we evaluated the effects of these 3 Trichoderma strains on preventing cucumber fusarium wilt through pot culture and greenhouse culture experiments. All 3 Trichoderma strains demonstrated higher control effects toward cucumber fusarium wilt than previous studies, with efficacies over 78%. Additionally, inoculation with the 3 Trichoderma strains significantly promoted the quality and yield of cucumbers. Among the 3 strains, Trichoderma 866 was the most effective, with disease control efficacy of 78.64% and a cucumber yield increase of 33%. Furthermore, seedlings inoculated with Trichoderma exhibited significantly increased measures of plant height, stem diameter, leaf area, aboveground fresh weight, underground fresh weight, chlorophyll content, and nitric nitrogen content, as well as the activities of several stress-resistance enzymes, including superoxide dismutase(SOD), peroxidase(POD), catalase(CAT), polyphenol oxidase(PPO), and ascorbate oxidase(AAO). In addition, the plants inoculated with Trichoderma showed decreased cell membrane permeability and malondialdehyde(MDA) content in the leaves. Together, our results suggest that T. asperellum 525, T. harzianum 610, and T. pseudokoningii 886 inoculations inhibit F. oxysporum infection, stimulate the metabolism in cucumbers, and enhance the activities of stress-resistance enzymes, which consequently promote the growth of cucumber plants, prevent cucumber fusarium wilt, and improve the yield and quality of cucumbers. T. harzianum is a commonly used biocontrol fungus, while few studies have focused on T. asperellum or T. koningense. In this study, strains of T. asperellum and T. pseudokoningii showed excellent plant disease prevention and growth promoting effects on cucumber, indicating that they also have great potential as biocontrol fungi.
基金supported by the National Natural Science Foundation of China(39830220)
文摘The amount of phenol compounds in the soil increased after adding organic material into the soil. It was found that p-hydroxybenzoic acid, p-coumaric acid and frulic acid alleviated Fusarium wilt of cucumber, the alleviating effect of p-hydroxybenzoic acid was the best, followed by p-coumaric acid and frulic acid. The total amount of bacterial, actinomyces and fungus in high phenol compounds treatment decreased than that of control treatment, while the microorganisms' amount in low phenol compounds treatment increased. Phenol compounds inhibit the growth of pathogen.
文摘Fusarium wilt caused by Fusarium oxysporum f.sp. cucumerinum (Owen) is one of the most devastating diseases in cucumber production worldwide. Recent progresses in the mechanism of resistance to Fusarium wilt in cucumber were reviewed in this paper, including pathogenic mechanism of Fusarium oxysporum, the resistance mechanism of cucumber, the heredity of resistance, and the location of resistance genes. Following works should be the location and cloning of resistance genes with molecular biologic methods.
基金Supported by Special Fund for Nonprofit Industry of Ministry of Agriculture(2011R20A24B02)Support Project of Science and Technology Ministry(2012BAC17B02-2-1)+1 种基金Nonprofit Agricultural Research Projects of Zhejiang Province(2010C32016)Provincial Major Science and Technology Projects(priority topics)for Agricultural Program(2008C12045-1)
文摘With conventional fertilization as the control,the control effects of biological fertilizer and general organic fertilizer on watermelon Fusarium wilt were studied in the paper. The results showed that applying biological fertilizer could effectively reduce the incidence rate of watermelon Fusarium wilt and significantly improve the quality of watermelon,while application of general organic fertilizer had no obvious control effect on watermelon Fusarium wilt.
文摘Sugar Baby susceptible to Fusarium oxysporum f. sp. niveum was crossed with high resistant male parents D3 - 1 and D3 - 2, respectively. F1 hybrids showed high resistance. The segregation ratios of resistance to susceptibility of F2 and of BC1 hybrid population from Sugar Baby tallied with 3: 1 and 1: 1, respectively. The results indicated that the resistance to Fusarium wilt was a kind of dominant inheritance controlled by mono- gene or mono- segment DNA. Furthermore, 5 hybrid combinations with fine character were bred. Among them, 3 were high resistant to Fusarium wilt,and 2 were medium res
