[Objectives] To examine the mechanisms underlying the anti-inflammatory, anti-pruritic, and anti-allergic effects of the Fuyanjie Chinese herbal formula, which comprises Radix Sophorae Flavescentis, Stemonae Radix, Fr...[Objectives] To examine the mechanisms underlying the anti-inflammatory, anti-pruritic, and anti-allergic effects of the Fuyanjie Chinese herbal formula, which comprises Radix Sophorae Flavescentis, Stemonae Radix, Fructus Cnidii, and Phellodendri Chinensis Cortex, on sensitive skin using network pharmacology.[Methods] The TCMSP database was employed to identify and extract the active ingredients and corresponding targets of the Fuyanjie formula. The collective targets were then intersected with the disease targets "pruritus", "dermatitis", and "skin allergy", which were obtained from the GeneCards database, in order to identify the core targets. The String database, Cytoscape software, and the cytoHubba plug-in were utilized to construct and analyze the protein-protein interaction (PPI) network, and the key hub genes were subsequently identified and quantified. A GO function analysis and KEGG enrichment analysis of core action targets were conducted utilizing the DAVID database.[Results] A total of 87 active ingredients were identified from the Fuyanjie formula through a screening process, which corresponded to a total of 254 targets of action. Through intersection analysis, 41 core targets of the Fuyanjie Chinese herbal formula were identified, which contribute to its anti-inflammatory, anti-pruritic, and anti-allergic effects. The findings indicated that quercetin, beta-sitosterol, stigmasterol, formononetin, luteolin, and other bioactive compounds present in the Fuyanjie Chinese herbal formula may interact with the targets IL-6, MMP-9, TNF, and IL-1β. These compounds were suggested to exert anti-inflammatory, anti-pruritic, and anti-allergic effects through pathways associated with the inflammatory response, including the IL-17 signaling pathway, TNF signaling pathway, NF-κB signaling pathway, etc.[Conclusions] Fuyanjie Chinese herbal formula may modulate skin conditions through a multifaceted mechanism of action that involves multiple components, targets, and pathways.展开更多
基金Supported by Shanghai Putuo District Science and Technology R&D Platform Project(2024QX04).
文摘[Objectives] To examine the mechanisms underlying the anti-inflammatory, anti-pruritic, and anti-allergic effects of the Fuyanjie Chinese herbal formula, which comprises Radix Sophorae Flavescentis, Stemonae Radix, Fructus Cnidii, and Phellodendri Chinensis Cortex, on sensitive skin using network pharmacology.[Methods] The TCMSP database was employed to identify and extract the active ingredients and corresponding targets of the Fuyanjie formula. The collective targets were then intersected with the disease targets "pruritus", "dermatitis", and "skin allergy", which were obtained from the GeneCards database, in order to identify the core targets. The String database, Cytoscape software, and the cytoHubba plug-in were utilized to construct and analyze the protein-protein interaction (PPI) network, and the key hub genes were subsequently identified and quantified. A GO function analysis and KEGG enrichment analysis of core action targets were conducted utilizing the DAVID database.[Results] A total of 87 active ingredients were identified from the Fuyanjie formula through a screening process, which corresponded to a total of 254 targets of action. Through intersection analysis, 41 core targets of the Fuyanjie Chinese herbal formula were identified, which contribute to its anti-inflammatory, anti-pruritic, and anti-allergic effects. The findings indicated that quercetin, beta-sitosterol, stigmasterol, formononetin, luteolin, and other bioactive compounds present in the Fuyanjie Chinese herbal formula may interact with the targets IL-6, MMP-9, TNF, and IL-1β. These compounds were suggested to exert anti-inflammatory, anti-pruritic, and anti-allergic effects through pathways associated with the inflammatory response, including the IL-17 signaling pathway, TNF signaling pathway, NF-κB signaling pathway, etc.[Conclusions] Fuyanjie Chinese herbal formula may modulate skin conditions through a multifaceted mechanism of action that involves multiple components, targets, and pathways.
