Chromosome G-banding in SituHybridization of RFLP Marker umc58 Linked with the Gene hm1 Dictating Helminthosporium carbonum Susceptibility1 in Maize

The technique of simultaneous G banding and in situ hybridization has been developed in plants for the first time.Using this technique.RFLP marker umc58 closely linked with the hm1 gene dictating Helminthosporium carbonum susceptibility1 was localized onto 1L3(chromosome 1,long arm,the third band from the centromere to the end of the arm),5L5 and 9L5.Theresults demonstrated that umc58 was a tripli cated sequence.It was deduced that umc58 probably was in a duplicated region that includes a part of Helminthosporium carbonum susceptibility genes(hm1 and hm2),as the hybridization sites of umc58 in chro mosomes 1 and 9 were those at which the genes localize.The techniques of simultaneous G banding and ISH in plants are discussed.

R-Band of Northeastern Sika Deer (Cervus nippon hortulorum) Chromosomes

Objeclive The aim of this study was to investigate R-band of Cervus nippon hortulorum chromosomes and to provide references for genetic variation and gene location of Cervus nippon hortulorum. [Metbod] Cell division was synchronized by the pepripheral blood lymphocyte culture and the excessive dosage of thymine deoxyribonucleoside, and R-band of Cervus nippon hortulorum chromosomes was also analyzed by RBG-banding technique. Result The number of haploid chromosome banding increased to 400. The R-band of No. 1, No. 2, No. 3, No. 4, chromosome X and Y were almost just opposite to the high-resolution G band of them. The terminal of chromosomes except No. 21, No. 24 and No. 28 were all pos- itive deeply stained. E Conclusion] R-band of Cervus nippon hortulorum chromosomes can be manifested by RBG-binding technique.

药用植物丹参的核型分析和C-banding研究

采用传统的核型分析技术，分析了栽培丹参有丝分裂中期染色体的核型和C—banding。结果表明：丹参中期染色体长度为1．30-2．39μm，核型公式是2n=16=4m＋2sm＋2st，其核型为2A型；单套染色体组显示出19条清晰稳定的C—bands，其中包括13条末端带，1条着丝粒带和5条中间带。

胎儿末端染色体非平衡易位遗传方式的CNV-seq联合G显带核型分析

目的:通过拷贝数变异检测(CNV-seq)联合G显带核型分析对产前诊断和流产的胎儿末端染色体非平衡易位发生频率以及遗传方式进行分析。方法:选取2018年6月至2021年12月在郑州大学第一附属医院经CNV-Seq判定为末端染色体非平衡易位的病例,采用外周血G显带核型分析或FISH检测对胎儿父母进行溯源分析。结果:17248例产前诊断和流产病例中,88例检出末端染色体非平衡易位,检出率为0.51%。其中59例行父母G显带核型分析或FISH检测,32例(54.24%)是由于父母为平衡易位导致,27例(45.76%)为新发变异。结论:诊断为末端染色体非平衡易位的病例,父母行G显带核型分析或FISH检测可提高染色体平衡易位携带者的检出率。

奶牛小肠上皮细胞系细胞染色体核型与G-带分析

为了鉴定奶牛小肠上皮细胞系(BIECs-21)的生物学遗传稳定性,体外贴壁培养BIECs-21细胞,制备染色体标本和G-带标本,分析BIECs-21细胞的染色体核型和G-带。结果表明:BIECs-21细胞染色体数为2n=60,包括常染色体29对和性染色体(X和Y染色体)1对。其中,29对常染色体均为端部着丝点染色体;性染色体中,X染色体是较大的中着丝粒染色体,Y染色体是较小的中着丝粒染色体。除X、Y性染色体外,BIECs-21细胞常染色体的G-带带纹的数量和位置无显著差异。BIECs-21细胞与荷斯坦牛的正常染色体核型相比,染色体数目及形态结构均没有明显变化,证明该细胞系遗传特性稳定,可以用于牛肠道相关疾病分子机制的研究。

5G广播电视新动向及启示

3GPP 5G广播电视标准颁布以来,引发了全世界的关注,诸多国家进行了基于5G广播电视技术的试验和探索。2023年以来,国际5G广播电视领域新动向不断:第一,9月份3GPP为5G广播电视定义了“Band 108”等专用频段和“SDO”工作模式;第二,6月份ETSI在其新版5G广播电视标准中增加了紧急广播系统的章节;第三,2023年7月份美国FCC批准了在波士顿地区开展5G广播电视试验的申请,2024年1月又收到了二期试验申请。本文逐一介绍了这些新动向的背景和概况,并分析了其对我国广电行业的启示。

G波段二次谐波准光回旋行波管研究

为开展适用于太赫兹频段的大功率回旋放大器研究。对基于准光共焦波导的二次谐波准光回旋行波管进行了研究,理论与仿真结果表明,准光波导具有良好的模型选择性,利用器件的二次谐波工作,其基波与二次谐波的返波振荡可以得到很好的抑制。在理论与仿真研究基础上,设计了一只G波段的二次谐波准光回旋行波管,理论计算结果表明:在65 kV电子束电压、10 A电流、速度横纵比为1、磁场强度为4.19 T时,最大输出功率为90 kW,效率为13.8%,增益为34.8 dB。当输入功率10 W时,-3 dB带宽为2 GHz。

Structural Changes of 2V Chromosome of Haynaldia villosa Induced by Gametocidal Chromosome 3C of Aegilops triuncialis

Haynaldia villosa （2n=2X= 14, VV）, a relative of wheat, plays important roles in wheat improvement mainly owing to its disease resistance. Powdery mildew resistance gene Pm21 has been successfully transferred into wheat by Cytogenetic Institute, Nanjing Agricultural University, China, and is widely used in the current wheat breeding programs. In this research, our objective is to further transfer and utilize the beneficial genes such as eye-spot resistance, yellow rust resistance, and gene of the tufted bristles on the glume ridge （a remarkable morphology） mapped on 2V of Haynaldia villosa. A disomic addition line with gametocidal chromosome 3C ofAegilops triuncialis added in Norin-26 was crossed to the wheat-H, villosa disomic substitution 2V（2D） and the hybrid F1 was then self-crossed. Chromosome C-banding, genomic in situ hybridization （GISH）, and meiotic analysis in combination with molecular markers were applied to detect the chromosome variations derived from hybrids Fz and F3. To date, four translocations including one small segmental translocation T6BS·6BL-2VS, two whole arm translocations （preliminarily designed as T3DS·2VL and T2VS.7DL） and one intercalary translocation T2VS·2VL-W-2VL, one deletion Del. 2VS·2VL-, one monotelosomic Mt2VS, and one isochromosome 2VS·2VS line have been developed and characterized. One wheat SSR marker Xwmc25.120 tagging 2VS and one wheat STS marker NAU/STSBCD135-1 （2BL） tagging 2VL were successfully used to confirm the alien chromosome segments involved in the seven lines. The tufted bristles on the glume ridge appeared in lines T2VS-7DL, Mt2VS, 2VS-2VS as well as the parent DS2V（2D）, whereas in T3DS·2VL, this trait did not appear. The gene controlling the tufted bristles was located on 2VS. Gametocidal chromosome 3C ofAegilops triuncialis could successfully induce chromosome 2V structural changes.

Intragastric band erosion:Experiences with gastrointestinal endoscopic removal

AIM:To remove the migrated bands using a gastrointestinal endoscopic approach.Little is published on complications that can occur.METHODS:From June 2006 to June 2010,eight patients developed intragastric band migration.Two patients had received their AGB in a different hospital,theremaining six were operated by the same surgeon.In all patients gastrointestinal endoscopic removal of the band was attempted by two individual gastroenterologists.Clinical signs of band migration were:persisted nausea,abdominal pain,weight gain,recurrent infection of the port and tubing system and hematemesis.RESULTS:In four patients removal was performed without complications.In two patients extracting the cleaved gastric band into the stomach appeared impossible.The two remaining patients presented with acute hematemesis and melena.One of these patients was readmitted with hematemesis.The other patient started bleeding during the gastroscopy and was converted to a laparoscopy in which one of the branches of the left gastric artery was oversewn.CONCLUSION:Band migration after gastric banding can be life threatening.Gastrointestinal endoscopic removal is a feasible technique that holds the promise of fast reconvalescence.

Molecular Profile of Human Serine Palmitoyltransferase-1 Proximate of Chromosome 9 Disease Susceptibility Gene Cluster in Inflammatory Cancer Cell Lines

Background: Over 1100 genes have been annotated for human chromosome 9, including disease genes implicated in inflammation, atherosclerosis, cancer and neurodegeneration. The serine palmitoyltransferase-1, SPTLC1, gene is at the 9q22.2 cytogenetic band, a high G+C content region with common genetic alterations sufficient to modify cellular behavior. The sequence is highly conserved among diverse species from bacteria to humans, including a recently discovered 126 nucleotide alternate open reading frame, AltORF. The protein encoded by the reading frames has domains of biological interest and considerable overlapping molecular functions associated with cellular behavior and cancer progression. Methods: Here we examined molecular features of SPTLC1 in a group of inflammation associated cancer cell lines SKN-SH, MDA-PCa, Glioma LN18, PC3 and 647V. Subcellular localization of SPTLC1 was assessed by immunofluorescence microscopy and recombinant green fluorescent protein expression. In addition, PCR, DNA sequencing and bioinformatics analysis were used for molecular profiling of the SPTLC1 genomic and reverse transcribed cDNA fragments. Results: SPTLC1 is detected in all cell lines examined, with intense peri-nuclear staining, consistent with localization in the cytoplasm. Genomic DNA sample, but not the cD NA of SKN cells could be amplified with an AltORF primer set. The PC3 and MDA-PCa cancer cell lines which are both of prostate origin, show differences in SPTLC1 PCR amplification. Similar levels of SPTLC1 AltORF transcripts were detected by quantitative RT-PCR in all cell lines, except the PC3 cell line with low transcript level whose cDNA did not generate nucleotide base sequence information. Conclusions: This is the first reported transcriptional expression of the SPTLC1 AltORF for the inflammation associated human cancer cell lines. Interestingly, it is proximate of oncogenic cancer susceptibility genes and distal of tumor suppressor genes, the high content of short nucleotide repeats in the SPTLC1 AltORF sequence suggesting the region may be genetically unstable. This nominal functional genomics report on the human SPTLC1 AltORF will contribute to compiling a more detailed SPTLC1 gene ontology and is expected to help shed more insight into unique molecular attributes of SPTLC1 in the context of cancer cell behavior, malignant progression and the design of treatment for inflammation associated cancers.

Eighteen-Element Antenna for Metal-Rimmed Smartphone Sub-6 GHz LTE42 Band Applications

Due to the limited space and large mutual coupling levels,the design of sub-6 GHz massive Multi-Input Multi-Output(m-MIMO)smartphone antenna system attracts antennas’designers and engineers worldwide.Therefore,this paper presents 18-element m-MIMO antenna system that covers the long-term evolution 42(LTE42)frequency band(3.4–3.6 GHz)for the fifth generation(5G)applications in metallic frame smartphones.The proposed array system is etched on the long sides of a metal rim of the mobile chassis symmetrically,which is electrically connected to the system ground plane with zero ground clearance.A low-profile frame of height 7 mm(λ/12.3)is symmetrically placed below&above the ground system level.The two frame parts(above/below the ground level)are utilized separately for the implantation of the antenna elements to achieve good space exploitation and perfect pattern diversity between elements.Orthogonal feeds are used to further increase the level of the isolation where each antenna element above the ground level is fed by a 50-ohm microstrip line,and each antenna element below the ground level is fed by a 50-ohm coplanar waveguide(CPW).The proposed antenna structure is a capacitive coupled-fed open-slot antenna with a small footprint area of 12×3.5 mm2(λ/7.2×λ/24.5,whereλrepresents the free space wavelength at 3.5 GHz).A small coupling L-shaped strip provides a capacitive coupling for the proposed 5G antenna structure.To establish the contribution of the proposed 18-element m-MIMO,the prototype of the proposed system was manufactured and successfully measured.Both measured and simulated results are shown to be in good agreement.This proves that the proposed antenna provides coverage for the LTE42 band(3.4–3.6 GHz)with acceptable isolation and efficiency.Moreover,the performance of the proposed m-MIMO were further verified via channel capacity calculation and the envelope correlation coefficient(ECC)measurement.Based on that,the proposed m-MIMO is shown to provide a desirable performance for all mMIMO parameters while it owns the largest MIMO order in mobile terminals in the open literature.

Dual Band Switched Beam Textile Antenna for 5G Wireless Communications

This paper presents the single element dual band switched beam textile antenna.The antenna can operate at frequencies of 0.7 and 2.6 GHz using for 5G wireless communication applications.Textile fabric is considered to be used for substrate layer at the parts of a microstrip antenna for wireless body area network.The beam pattern of antenna can be switched into two directions by changing the position of shorted-circuit points at each edge of antenna.The main beam direction is 45°/225°when corner A is shorted while it steers at 135°/315°when corner B is shorted circuit.The advantage of the proposed antenna is the decrease of the problems like interference,light weight,flexibility and ability to switch beam easily.In addition,the results of the fabricated antenna are compared with the simulated ones.Moreover,the antenna is bent with curvature radius of 6 mm in forward direction.The effects of the bent antenna are studied.The results can confirm that radiation patterns of the bending antenna can be pointed into two directions when changing the positions of shorted circuit.Therefore,the proposed antenna can switch beam patterns,it is flexible,and it can operate at dual-band frequency on textile.

The Interacting Boson Model for Anomalous Rotational Bands

The interacting boson model for anomalous rotational bands is proposed. In the rotational SU(3) limit,an asymptotic limit is discussed. Within the framework of the model several analytic relations for energies and electro-magnetic transition rates are derived.

Genetic analysis of transgenome structure and size of chromosome-mediated gene transfer lines

The TK-selected chromosome-mediate gene transferlines were analysed using DNA dot blot method,G-11banding and in situ hybridization.The results showedthat CMGT can provide a wide variety of intermediatesize of the transgenome from greater than 80,000kb toless than 2,000kb.Some of transfectants are intergratedinto mouse chromosome which can be detected by G-11banding and in situ

EXPLICIT SOLUTION FOR G-BAND MODE FREQUENCY OF SINGLE-WALLED CARBON NANOTUBES

G-band mode is one of the most important Raman modes of single-walled carbon nanotubes （SWCNTs）. The vibrational frequency of the mode can be used to characterize SWC- NTs. However, analytical expression that can link the frequency to the geometrical parameters of a SWCNT is to date not reported. Based on a molecular mechanics model, the analytical solution is obtained for G-band mode frequency of SWCNTs. The result calculated from the present solutions is in good agreement with the existing experimental and numerical data.

The system of L-band 2 × 10 Gb/s WDM transmission over conventional single mode fibre with 600 km by chirped fibre Bragg gratings dispersion compensation

A chirped fibre Bragg grating according to ITU-T suggested L-band （2nd channel λ1 = 1570.83 nm; 80th channel λ2 = 1603.57 nm） with more than 1800 ps/nm single channel dispersion compensation is presented in this paper, of which the cladding mode loss, the delay curve ripple and the power fluctuation of the reflected spectrum are less than 0.5 dB, 50 ps and 0.25 dB, respectively. With this new FBG as dispersion compensation device, a 2 × 10 Gb/s wavelength division multiplexing （WDM） L-band transmission of 600 km based on conventional single mode fibre （G.652 fibre） is performed without forward error correction. The bit error rate （BER） is less than 10-12 and the power penalties of the 2nd and 80th channel of L-band are 1.8 dB and 2.0 dB, respectively.

STUDY OF RADIO DEVICES ON 2.4GHZ ISM BAND IN PHYSICAL PHASE

In the unlicensed 2.4GHz ISM band,there is not only IEEE 802.11 wireless systems being used,but also some other devices,such as residential microwave oven,Bluetooth devices,and cordless phone.All these devices that are not used for the data communica- tions cause unintentional interference that will degrade the WI-FI system.In this paper,the transmission powers for common RF devices are measured and the SIR(signal-to-interference ratio)of different interferers to Wi-Fi is also studied.With this information, the effect of different interference on WI-FI signal can hence be quantified.Furthermore,the ability of the interference avoidance protocols,which is built into the AP,to address interference problems caused by that device is determined.

血清sHLAG、PGⅠ、PGⅡ与早期胃癌的关系及与窄带成像技术的联合诊断价值

目的 探讨血清可溶性人白细胞抗原-G(sHLAG)、胃蛋白酶原Ⅰ(PGⅠ)、胃蛋白酶原Ⅱ(PGⅡ)与早期胃癌的关系及与窄带成像技术(NBI)的联合诊断价值。方法 选取2019年3月-2023年4月于沧州市人民医院诊治的182例胃黏膜病变患者作为研究对象,经组织病理检查确诊为胃癌80例,胃良性病变102例。在病理确诊前行血清sHLAG、PGⅠ、PGⅡ和NBI检查。结果 胃癌组血清sHLAG、PGⅡ高于胃良性病变组,而PGⅠ低于胃良性病变组(P<0.05)。不同TNM、分化程度、肿瘤直径、浸润程度胃癌患者的sHLAG、PGⅠ、PGⅡ比较,差异有统计学意义(P<0.05)。Kendall′s tau-b相关性分析显示,sHLAG、PGⅡ与TNM、分化程度、肿瘤直径、浸润程度呈正相关(P<0.05);PGⅠ与TNM、分化程度、肿瘤直径、浸润程度呈负相关(P<0.05)。4项联合诊断准确率、敏感度分别高于sHLAG、PGⅠ、PGⅡ、NBI单独诊断(P<0.05)。结论 血清sHLAG、PGⅠ、PGⅡ在早期胃癌患者中呈异常表达,且与NBI联合有利于提高早期胃癌的诊断效能。

非染色体结构维护凝集素Ⅰ复合物亚基G通过Akt信号通路促进卵巢癌细胞的增殖、迁移和侵袭

目的探讨非染色体结构维护凝集素Ⅰ复合物亚基G(NCAPG)调控卵巢癌细胞的增殖、迁移和侵袭的机制。方法生物信息学GEPIA数据库分析NCAPG在卵巢癌组织中的差异表达。Western blotting检测正常卵巢上皮细胞IOSE80、卵巢癌A2780和SKOV3细胞中NCAPG的蛋白表达。NCAPG siRNA沉默实验分为空白组、对照组、siNCAPG-1组和siNCAPG-2组。NCAPG过表达实验分为空白组、对照组、NCAPG组、NCAPG+MK2206组和MK2206组。MTT实验检测细胞增殖活性;细胞划痕实验和Transwell实验评估细胞的迁移和侵袭能力;Western blotting检测细胞的磷酸化Akt(p-Akt)、总Akt(t-Akt)、增殖细胞核抗原(PCNA)、基质金属蛋白酶9(MMP-9)、波形蛋白(vimentin)、N-钙黏蛋白(N-cadherin)和E-钙黏蛋白(E-cadherin)的表达水平。结果NCAPG在卵巢癌组织和卵巢癌细胞中高表达。沉默NCAPG可明显抑制卵巢癌SKOV3细胞的增殖、迁移和侵袭,p-Akt、PCNA、MMP-9、vimentin和N-cadherin表达减少,E-cadherin表达增多。过表达NCAPG质粒可促进卵巢癌A2780细胞增殖、迁移和侵袭,p-Akt、PCNA、MMP-9、vimentin和N-cadherin表达增多,E-cadherin表达降低。Akt抑制剂MK2206可明显抑制NCAPG的上述作用。结论NCAPG激活Akt信号通路,调控PCNA、MMP-9和上皮细胞-间充质转化(EMT)相关蛋白的表达,促进卵巢癌细胞的增殖、迁移和侵袭。

遥感卫星地面站12米口径天线Ka频段G/T值测试方法综述

Ka频段G/T值是遥感卫星地面站12米口径天线最重要的指标之一,其测试方法与遥感站目前常用的X频段测试方法相比,适用条件更加严苛,因此如何选取最佳的测试方法成为本文研究的主要内容。首先介绍了Ka频段12米口径天线特点;然后分别论述了分段测试法、信标法、比较法和射电源法的原理及其优缺点,并提出了Ka频段对月测试的这一最方便简洁的测试方法及其修正系数的研究方向;最后通过实测结果进行比较,给出Ka频段G/T值测试方法选取建议,为工程师现场测试提供了科学的指导和参考。