目的通过观察地高辛(Digoxin)干预血管紧张素Ⅱ(AngⅡ)诱导的ApoE-/-小鼠高血压性心肌肥厚模型后,对G蛋白调节因子2(regulator of G protein signaling 2,RGS2)的影响,探讨地高辛治疗高血压性心肌肥厚的作用与可能的机制。方法 30只雄性...目的通过观察地高辛(Digoxin)干预血管紧张素Ⅱ(AngⅡ)诱导的ApoE-/-小鼠高血压性心肌肥厚模型后,对G蛋白调节因子2(regulator of G protein signaling 2,RGS2)的影响,探讨地高辛治疗高血压性心肌肥厚的作用与可能的机制。方法 30只雄性ApoE-/-小鼠随机分为对照组、AngⅡ模型组和AngⅡ+Digoxin治疗组。所有小鼠从术前1d至术后处死前均接受0.5%二甲基亚砜或地高辛溶液腹腔注射治疗,并且在术后28d获取心脏组织,通过组织学检查、苏木精-伊红(HE)染色切片分析技术评定心肌组织形态学变化、RGS2的mRNA及蛋白表达水平来评价地高辛的作用。结果与AngⅡ模型组相比,AngⅡ+Digoxin组全心重量、左心室壁厚度、心肌细胞直径均明显减少(P<0.05或P<0.01),RGS2mRNA变化不明显,而蛋白表达增高(P<0.01),心肌肥厚明显减轻。同时检测小鼠在术前3d,手术当天,术后3、7、14、28d的血压,发现AngⅡ+Digoxin组与AngⅡ模型组比较,术后7d和14d血压下降(均P<0.05),术后28d则差异无统计学意义。结论地高辛可能通过上调ApoE-/-小鼠高血压心肌肥厚模型RGS2的表达,有效减轻心肌肥厚,这说明地高辛可能在抑制心肌细胞肥大中起重要作用。展开更多
Large-scale electric vehicles(EVs) connected to the micro grid would cause many problems. In this paper, with the consideration of vehicle to grid(V2 G), two charging and discharging load modes of EVs were constructed...Large-scale electric vehicles(EVs) connected to the micro grid would cause many problems. In this paper, with the consideration of vehicle to grid(V2 G), two charging and discharging load modes of EVs were constructed. One was the disorderly charging and discharging mode based on travel habits, and the other was the orderly charging and discharging mode based on time-of-use(TOU) price;Monte Carlo method was used to verify the case. The scheme of the capacity optimization of photovoltaic charging station under two different charging and discharging modes with V2 G was proposed. The mathematical models of the objective function with the maximization of energy efficiency, the minimization of the investment and the operation cost of the charging system were established. The range of decision variables, constraints of the requirements of the power balance and the strategy of energy exchange were given. NSGA-Ⅱ and NSGA-SA algorithm were used to verify the cases, respectively. In both algorithms, by comparing with the simulation results of the two different modes, it shows that the orderly charging and discharging mode with V2 G is obviously better than the disorderly charging and discharging mode in the aspects of alleviating the pressure of power grid, reducing system investment and improving energy efficiency.展开更多
文摘目的通过观察地高辛(Digoxin)干预血管紧张素Ⅱ(AngⅡ)诱导的ApoE-/-小鼠高血压性心肌肥厚模型后,对G蛋白调节因子2(regulator of G protein signaling 2,RGS2)的影响,探讨地高辛治疗高血压性心肌肥厚的作用与可能的机制。方法 30只雄性ApoE-/-小鼠随机分为对照组、AngⅡ模型组和AngⅡ+Digoxin治疗组。所有小鼠从术前1d至术后处死前均接受0.5%二甲基亚砜或地高辛溶液腹腔注射治疗,并且在术后28d获取心脏组织,通过组织学检查、苏木精-伊红(HE)染色切片分析技术评定心肌组织形态学变化、RGS2的mRNA及蛋白表达水平来评价地高辛的作用。结果与AngⅡ模型组相比,AngⅡ+Digoxin组全心重量、左心室壁厚度、心肌细胞直径均明显减少(P<0.05或P<0.01),RGS2mRNA变化不明显,而蛋白表达增高(P<0.01),心肌肥厚明显减轻。同时检测小鼠在术前3d,手术当天,术后3、7、14、28d的血压,发现AngⅡ+Digoxin组与AngⅡ模型组比较,术后7d和14d血压下降(均P<0.05),术后28d则差异无统计学意义。结论地高辛可能通过上调ApoE-/-小鼠高血压心肌肥厚模型RGS2的表达,有效减轻心肌肥厚,这说明地高辛可能在抑制心肌细胞肥大中起重要作用。
基金Project(3502Z20179026)supported by Xiamen Science and Technology Project,China。
文摘Large-scale electric vehicles(EVs) connected to the micro grid would cause many problems. In this paper, with the consideration of vehicle to grid(V2 G), two charging and discharging load modes of EVs were constructed. One was the disorderly charging and discharging mode based on travel habits, and the other was the orderly charging and discharging mode based on time-of-use(TOU) price;Monte Carlo method was used to verify the case. The scheme of the capacity optimization of photovoltaic charging station under two different charging and discharging modes with V2 G was proposed. The mathematical models of the objective function with the maximization of energy efficiency, the minimization of the investment and the operation cost of the charging system were established. The range of decision variables, constraints of the requirements of the power balance and the strategy of energy exchange were given. NSGA-Ⅱ and NSGA-SA algorithm were used to verify the cases, respectively. In both algorithms, by comparing with the simulation results of the two different modes, it shows that the orderly charging and discharging mode with V2 G is obviously better than the disorderly charging and discharging mode in the aspects of alleviating the pressure of power grid, reducing system investment and improving energy efficiency.
