In this report, we present a method for the detection of Pb2+ based on the different adsorption capacity on the surface of gold nanoparticles (AuNPs) between ssDNA (single-stranded DNA) and G-quartet. In the absence o...In this report, we present a method for the detection of Pb2+ based on the different adsorption capacity on the surface of gold nanoparticles (AuNPs) between ssDNA (single-stranded DNA) and G-quartet. In the absence of Pb2+, the DNA oligonucleotides probe, which is guanine-rich ssDNA, can be adsorbed on the surface of AuNPs protecting them from aggregation. After adding Pb2+, the DNA oligonucleotides probe can specifically form compact G-quartet, which can induce the aggregation of unmodified AuNPs, especially after adding NaCl aqueous solution. Consequently, the color turns from red to blue. Pb2+ can be detected by colorimetric response of AuNPs;its detection limit can reach 5 μM only observed by naked eyes. Most metal ions have no interferences, and the interference of Cu2+ can be effectively eliminated by adding cysteine. It provides a simple and effective colorimetric sensor for on-site and real time detection of Pb2+.展开更多
While 8-oxo-7,8-dihydro-2′-deoxyguanosine(dOG)on DNA G-quadruplex(G4)has been studied,the influence of 8-oxo-7,8-dihydro-guanosine(rOG)lesions on telomeric repeat-containing RNA(TERRA)G4 deserves an in-depth study.Th...While 8-oxo-7,8-dihydro-2′-deoxyguanosine(dOG)on DNA G-quadruplex(G4)has been studied,the influence of 8-oxo-7,8-dihydro-guanosine(rOG)lesions on telomeric repeat-containing RNA(TERRA)G4 deserves an in-depth study.The single-strand and guanine-rich characters of TERRA make it vulnerable to form rOG lesions.Our current study demonstrated that rOG located in the internal layer and external layer of TERRA impacted the G4 stability in different ways and perturbed RNA replication,as well as base-pair strength and stability.展开更多
文摘In this report, we present a method for the detection of Pb2+ based on the different adsorption capacity on the surface of gold nanoparticles (AuNPs) between ssDNA (single-stranded DNA) and G-quartet. In the absence of Pb2+, the DNA oligonucleotides probe, which is guanine-rich ssDNA, can be adsorbed on the surface of AuNPs protecting them from aggregation. After adding Pb2+, the DNA oligonucleotides probe can specifically form compact G-quartet, which can induce the aggregation of unmodified AuNPs, especially after adding NaCl aqueous solution. Consequently, the color turns from red to blue. Pb2+ can be detected by colorimetric response of AuNPs;its detection limit can reach 5 μM only observed by naked eyes. Most metal ions have no interferences, and the interference of Cu2+ can be effectively eliminated by adding cysteine. It provides a simple and effective colorimetric sensor for on-site and real time detection of Pb2+.
基金supported by the National Natural Science Foundation of China(nos.21722803,91853119,21572169,21721005,91753201,21877086 and 21672165)the Hubei Natural Science Foundation for Distinguished Young Scholars(2019CFA064)+2 种基金the National Major Scientific and Technological Special Project for“Significant New Drugs Development”(2017ZX09303013)the Fundamental Research Funds for the Central Universities(2042019kf0189)the Natural Science Innovation Foundation of Wuhan University.
文摘While 8-oxo-7,8-dihydro-2′-deoxyguanosine(dOG)on DNA G-quadruplex(G4)has been studied,the influence of 8-oxo-7,8-dihydro-guanosine(rOG)lesions on telomeric repeat-containing RNA(TERRA)G4 deserves an in-depth study.The single-strand and guanine-rich characters of TERRA make it vulnerable to form rOG lesions.Our current study demonstrated that rOG located in the internal layer and external layer of TERRA impacted the G4 stability in different ways and perturbed RNA replication,as well as base-pair strength and stability.
