At present,there are unharmonious factors existing in Chinese family education,which particular manifest in the means of family education.The Three Character Primer,as an epitome of the essence of Chinese traditional ...At present,there are unharmonious factors existing in Chinese family education,which particular manifest in the means of family education.The Three Character Primer,as an epitome of the essence of Chinese traditional culture,can provide enlightenment on building a democratic and harmonious family education means.展开更多
Objective Using homology primers for clone of β-chemokine receptor supergene-family from lower level RNA expression. Methods: Through similarity alignment of the conservative transmembrane domainof human MCP-l recept...Objective Using homology primers for clone of β-chemokine receptor supergene-family from lower level RNA expression. Methods: Through similarity alignment of the conservative transmembrane domainof human MCP-l receptor, mouse MIP-lα receptor and rat IL-8 receptor. the degenerate oligonucleotide homology primers were designed and a cDNA fragment was amplified by RT--PCR. After a new gene sequenceof chemokine receptor supergene-family in GenBank was identified, the new gene full sequence was cloned bya Marathon PCR of the specific primers. Results: Mouse CCR5 full-length cDNA with 2888 bp was cloned,including open reading frame 1065 oligonucleotides and coding 355 amino acids. This gene was a novel member of the β-chemokine receptor supergene-family confirmed by sequence analysis, ligand binding assay andinforming from GenBank. Conclusion: Using homology primers, the novel member of the supergene-familycan be captured. This method is a new cloning strategy and has extensive significance in the molecular cloningof a new gene.展开更多
文摘At present,there are unharmonious factors existing in Chinese family education,which particular manifest in the means of family education.The Three Character Primer,as an epitome of the essence of Chinese traditional culture,can provide enlightenment on building a democratic and harmonious family education means.
文摘Objective Using homology primers for clone of β-chemokine receptor supergene-family from lower level RNA expression. Methods: Through similarity alignment of the conservative transmembrane domainof human MCP-l receptor, mouse MIP-lα receptor and rat IL-8 receptor. the degenerate oligonucleotide homology primers were designed and a cDNA fragment was amplified by RT--PCR. After a new gene sequenceof chemokine receptor supergene-family in GenBank was identified, the new gene full sequence was cloned bya Marathon PCR of the specific primers. Results: Mouse CCR5 full-length cDNA with 2888 bp was cloned,including open reading frame 1065 oligonucleotides and coding 355 amino acids. This gene was a novel member of the β-chemokine receptor supergene-family confirmed by sequence analysis, ligand binding assay andinforming from GenBank. Conclusion: Using homology primers, the novel member of the supergene-familycan be captured. This method is a new cloning strategy and has extensive significance in the molecular cloningof a new gene.
